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Team:Heidelberg/Notebook/Sensing Group/Notebook/6thweek
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* Miniprep of Liquid Culture from previous Transformation (done by Chenchen). Incubated for two days @ RT and about 6 h @ 37 °C | * Miniprep of Liquid Culture from previous Transformation (done by Chenchen). Incubated for two days @ RT and about 6 h @ 37 °C | ||
* Digestion of the Miniprep products with xbaI (Buffer2 + BSA, 1 ul enzyme @ 37 °C about 40min) | * Digestion of the Miniprep products with xbaI (Buffer2 + BSA, 1 ul enzyme @ 37 °C about 40min) | ||
| - | + | [[Image:HD 080908-LuxQ Colonies-Digestion xbaI.png|center|thumb|500px|Digestion of LuxQ with xbaI yielded expected bands (4736 + 1204 bp) for all samples, except for no. 4]] | |
| - | [[Image:HD 080908-LuxQ Colonies-Digestion xbaI.png|center|thumb|500px|Digestion of LuxQ with xbaI yielded expected bands for all samples, except for no. 4]] | + | |
== Tuesdsay, 09/09/2008 == | == Tuesdsay, 09/09/2008 == | ||
Revision as of 16:51, 26 October 2008
Contents |
Monday, 09/08/2008
- Liquid Culture of 5 colonies from LuxQ Transformation and 1 from F1 transformation
- Miniprep of Liquid Culture from previous Transformation (done by Chenchen). Incubated for two days @ RT and about 6 h @ 37 °C
- Digestion of the Miniprep products with xbaI (Buffer2 + BSA, 1 ul enzyme @ 37 °C about 40min)
