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Team:Heidelberg/Notebook/Sensing Group/Notebook/6thweek
From 2008.igem.org
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* Digestion of the Miniprep products with xbaI (Buffer2 + BSA, 1 ul enzyme @ 37 °C about 40min) | * Digestion of the Miniprep products with xbaI (Buffer2 + BSA, 1 ul enzyme @ 37 °C about 40min) | ||
[[Image:HD 080908-LuxQ Colonies-Digestion xbaI.png|center|thumb|500px|Digestion of LuxQ with xbaI yielded expected bands (4736 + 1204 bp) for all samples, except for no. 4]] | [[Image:HD 080908-LuxQ Colonies-Digestion xbaI.png|center|thumb|500px|Digestion of LuxQ with xbaI yielded expected bands (4736 + 1204 bp) for all samples, except for no. 4]] | ||
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| + | Sequencing @ GATC yielded correct sequence for LuxQ no. 1 | ||
| + | * PCR for LuxQ and LuxP with Phusion Mastermix | ||
| + | [[Image:HD 080908-LuxQ P PCR.png|center|thumb|400px|PCR for LuxQ and LuxP. LuxQ PCR did not work, but first PCR for LuxP was positive.]] | ||
== Tuesdsay, 09/09/2008 == | == Tuesdsay, 09/09/2008 == | ||
Revision as of 16:54, 26 October 2008
Contents |
Monday, 09/08/2008
- Liquid Culture of 5 colonies from LuxQ Transformation and 1 from F1 transformation
- Miniprep of Liquid Culture from previous Transformation (done by Chenchen). Incubated for two days @ RT and about 6 h @ 37 °C
- Digestion of the Miniprep products with xbaI (Buffer2 + BSA, 1 ul enzyme @ 37 °C about 40min)
Sequencing @ GATC yielded correct sequence for LuxQ no. 1
- PCR for LuxQ and LuxP with Phusion Mastermix
