Team:UNIPV-Pavia/Notebook/Week14

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==Week 11: 08/21/08 - 08/22/08==
==Week 11: 08/21/08 - 08/22/08==
-
'''08/4/08'''
+
'''08/21/08'''
<br>
<br>
*Sequencing results:
*Sequencing results:
-
** 27 - wrong sequence
+
**J23100-B0030-C0012-B1006-'''R0010''' - completely wrong sequence
-
**BBa_C0062 - OK
+
**C0062 - OK
-
**12 - OK
+
**Lig.12 - OK
-
**22 - point mutation
+
**Lig.22 - point mutation C->T
-
**30 - point mutation
+
**Lig.30 - point mutation C->T
-
*We decided to re-perform 34 and 35 assembly from 12, whose sequence was correct.
+
*We decided to restart from Lig.12, whose sequence was correct, to perform ligations Lig.22, Lig.30, Lig.34 and Lig.35.
 +
 
 +
*We prepared 0.5 l of LB + Amp for plates.
 +
 
 +
*We infected 9 ml LB + Amp with 30 µl of B0030-C0061-B1006-'''R0062'''-B0030-E0040-B1006 and Lig.12 glycerol stocks.
 +
 
 +
'''08/22/08'''
 +
<br>
 +
*Glycerol stocks/miniprep for B0030-C0061-B1006-'''R0062'''-B0030-E0040-B1006 and Lig.12.
 +
 
 +
*We planned experiments for qualitative and quantitative tests.

Latest revision as of 21:27, 26 October 2008


Home.jpg Home Unipv logo.jpg The Team And.jpg The Project Safety.jpg Biological Safety Dna.png Parts Submitted to the Registry
Laptop.jpg Dry Lab Pipette.jpg Wet Lab Math.gif Modeling Note.jpg Protocols Notebook.gif Activity Notebook



Notebook



Week 1 Week 2 Week 3 Week 4 Week 5 Week 6 Week 7
Week 8 Week 9 Week 10 Week 11 Week 12 Week 13 Week 14
Week 15 Week 16 Week 17 Week 18 Week 19 Week 20 Week 21
Week 22 Week 23 Week 24



Week 11: 08/21/08 - 08/22/08

08/21/08

  • Sequencing results:
    • J23100-B0030-C0012-B1006-R0010 - completely wrong sequence
    • C0062 - OK
    • Lig.12 - OK
    • Lig.22 - point mutation C->T
    • Lig.30 - point mutation C->T
  • We decided to restart from Lig.12, whose sequence was correct, to perform ligations Lig.22, Lig.30, Lig.34 and Lig.35.
  • We prepared 0.5 l of LB + Amp for plates.
  • We infected 9 ml LB + Amp with 30 µl of B0030-C0061-B1006-R0062-B0030-E0040-B1006 and Lig.12 glycerol stocks.

08/22/08

  • Glycerol stocks/miniprep for B0030-C0061-B1006-R0062-B0030-E0040-B1006 and Lig.12.
  • We planned experiments for qualitative and quantitative tests.
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