Team:Paris/Notebook/Freezer

From 2008.igem.org

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{{Paris/Menu}}
{{Paris/Menu}}
-
== Last number ==
+
= Last number used=
-
{|border="2"
+
{|border="2" style="text-align: center"
-
|Last Ligation number used
+
|'''Miniprep'''
 +
|'''Strain'''
 +
|'''Ligation'''
 +
|'''Digestion'''
 +
|'''PCR'''
|-
|-
-
|185
+
|216
 +
|216
 +
|234
 +
|202
 +
|154
|}
|}
-
{|border="2"
+
''(last checking on 19th october)''
-
|Last PCR number used
+
-
|-
+
-
|
+
-
|}
+
-
 
+
-
{|border="2"
+
-
|Last Digestion number used
+
-
|-
+
-
|
+
-
|}
+
-
 
+
-
{|border="2"
+
-
|Last Miniprep number used
+
-
|-
+
-
|
+
-
|}
+
-
 
+
-
== Last digestion number ==
+
-
{|border="2"
+
-
|Last digestion number used
+
-
|-
+
-
|
+
-
|}
+
 +
=Freezer=
== PCR products ==
== PCR products ==
{|
{|
Line 82: Line 68:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | PCR127
| style="background: #D4E2EF;" | PCR127
-
|align="center"| flhDC
+
|align="center"| FlhDC
|align="center"|O132
|align="center"|O132
|align="center"|O131
|align="center"|O131
Line 126: Line 112:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | PCR131
| style="background: #D4E2EF;" | PCR131
-
|align="center"| flhD rbs-
+
|align="center"| FlhD rbs-
|align="center"|O132
|align="center"|O132
|align="center"|O133
|align="center"|O133
Line 148: Line 134:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | PCR133
| style="background: #D4E2EF;" | PCR133
-
|align="center"| flhDC + prom
+
|align="center"| FlhDC + prom
|align="center"|O110   
|align="center"|O110   
|align="center"|O131
|align="center"|O131
Line 159: Line 145:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | PCR134
| style="background: #D4E2EF;" | PCR134
-
|align="center"| flhDC + prom
+
|align="center"| FlhDC + prom
|align="center"|O111  
|align="center"|O111  
|align="center"|O131
|align="center"|O131
Line 170: Line 156:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | PCR135
| style="background: #D4E2EF;" | PCR135
-
|align="center"| pfliL
+
|align="center"| pFliL
|align="center"|O124  
|align="center"|O124  
|align="center"|O125
|align="center"|O125
Line 214: Line 200:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | PCR139
| style="background: #D4E2EF;" | PCR139
-
|align="center"| pflhDC (URI)
+
|align="center"| pFlhDC (URI)
|align="center"| O 110
|align="center"| O 110
|align="center"| O 113
|align="center"| O 113
Line 225: Line 211:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | PCR140
| style="background: #D4E2EF;" | PCR140
-
|align="center"| flhDC (RBS -)
+
|align="center"| FlhDC (RBS -)
|align="center"| O 132
|align="center"| O 132
|align="center"| O 131
|align="center"| O 131
Line 280: Line 266:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | PCR145
| style="background: #D4E2EF;" | PCR145
-
|align="center"| PCRFliA1
+
|align="center"| FliA1
|align="center"| O 148
|align="center"| O 148
|align="center"| O 152
|align="center"| O 152
Line 291: Line 277:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | PCR146
| style="background: #D4E2EF;" | PCR146
-
|align="center"| PCRFliA2
+
|align="center"| FliA2
|align="center"| O 153
|align="center"| O 153
|align="center"| O 150
|align="center"| O 150
Line 321: Line 307:
|align="center"| yes
|align="center"| yes
|align="center"| 101
|align="center"| 101
 +
|align="center"|[[Team:Paris/August 26|August 26]]
 +
|- style="background: #dddddd;" 
 +
| style="background: #D4E2EF;" | PCR 149
 +
|align="center"| pFlhDC (Soutourina +RBS)
 +
|align="center"| O 154
 +
|align="center"| O 113
 +
|align="center"| MG1655
 +
|align="center"| 450 bp
 +
|align="center"|
 +
|align="center"| yes
 +
|align="center"|
|align="center"|[[Team:Paris/August 26|August 26]]
|align="center"|[[Team:Paris/August 26|August 26]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 368: Line 365:
|}
|}
-
== Plasmids: MiniPrep Products ==
+
== Parts ==
-
 
+
{|
{|
|- style="background: #649CD7;"  
|- style="background: #649CD7;"  
-
! colspan="8" style="background: #649CD7;" | Plasmids: MiniPrep Products 2008
+
! colspan="8" style="background: #649CD7;" | Ligations: Constructions realised
|- style="background: #649CD7; text-align: center;"
|- style="background: #649CD7; text-align: center;"
-
|width=5%| Number
+
|width=8%| Name/N°
-
|width=7.5%| Name
+
|width=8%| Plasmid
-
|width=7.5%| Plamsid
+
|width=7%| Strain
-
|width=25%| Biobricks
+
|width=7%| Miniprep
-
|width=25%| Description
+
|width=32%|Description
-
|width=10%| Products in use/comments
+
|width=20%| Digestion
-
|width=5%| Conc. ug/mL
+
|width=8%| comment
-
|width=15%| Date
+
|width=10%| Date
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP100
+
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_I0500 I0500]
-
|align="center"|[http://partsregistry.org/Part:BBa_B0034 B0034 ]
+
|align="center"|[http://partsregistry.org/Part:pSB2K3 pSB2K3]
-
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]  
+
|align="center"|S103
-
|align="center"|[[Image:Part_icon_rbs.png]]
+
|align="center"|MP119
-
|align="center"|Strongest RBS (Efficiency = 1)
+
|align="center"|[[Image:Part_icon_regulatory.png]]<br>araC/pBad
|
|
|
|
-
|align="center"|[[Team:Paris/July 26|July 26]]
+
|align="center"|[[Team:Paris/July 24|July 24]], [[Team:Paris/July 26|July 26]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP101.1
+
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_E0840 E0840]
-
|rowspan="3" align="center" |[http://partsregistry.org/Part:BBa_J23101 J23101]
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-
|rowspan="3" align="center" |[http://partsregistry.org/Part:BBa_J61002 pJ61002 ]
+
|align="center"|S106
-
|rowspan="3" align="center"|[[Image:Part_icon_regulatory.png]]
+
|align="center"|MP122
-
|rowspan="3" align="center"|Strongest Constitutive promoter
+
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>GFP generator; strong rbs
-
|rowspan="3" |July 26
+
-
|align="center"|333
+
-
|rowspan="3" align="center"|[[Team:Paris/July 22|July 22]], [[Team:Paris/July 23|July 23]], [[Team:Paris/July 26|July 26]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #FF9999;" |MP101.2
+
-
|style="background: #FF9999;"|finished
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |MP101.4
+
-
|align="center"|200
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |MP102
+
-
|align="center"|[http://partsregistry.org/Part:BBa_J23109 J23109]  
+
-
|align="center"|[http://partsregistry.org/Part:BBa_J61002 pJ61002]  
+
-
|align="center"|[[Image:Part_icon_regulatory.png]]
+
-
|align="center"|Weak Constitutive promoter
+
-
|July 26
+
|
|
-
|align="center"|[[Team:Paris/July 22|July 22]], [[Team:Paris/July 26|July 26]]
+
|align="center"|
 +
|align="center"|[[Team:Paris/July 26|July 26]]
 +
|- style="background: #dddddd;"
 +
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_B0034 B0034 ]
 +
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
 +
|align="center"|S107
 +
|align="center"|MP100
 +
|align="center"|[[Image:Part_icon_rbs.png]]<br>Strongest RBS (Efficiency = 1)
 +
|
 +
|
 +
|align="center"|[[Team:Paris/July 26|July 26]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP103
+
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_R0079 R0079]
-
|align="center"|[http://partsregistry.org/Part:BBa_R0079 R0079]
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-
|align="center"|[[Image:Part_icon_regulatory.png]]
+
|align="center"|S108
-
|align="center"|promoter pLas (lasr & PAI regulated)
+
|align="center"|MP103
-
|July 26
+
|align="center"|[[Image:Part_icon_regulatory.png]]<br>pLas (lasr & PAI regulated)
 +
|
|
|
|align="center"|[[Team:Paris/July 23|July 23]], [[Team:Paris/July 26|July 26]]
|align="center"|[[Team:Paris/July 23|July 23]], [[Team:Paris/July 26|July 26]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP104.1
+
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_J23101 J23101]
-
|rowspan="3" align="center"|[http://partsregistry.org/Part:BBa_R0040 R0040]
+
|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002 ]
-
|rowspan="3" align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
|align="center"|S109
-
|rowspan="3" align="center"|[[Image:Part_icon_regulatory.png]]
+
|align="center"|MP101
-
|rowspan="3" align="center"|tetR repressible promoter
+
|align="center"|[[Image:Part_icon_regulatory.png]]<br>Strongest Constitutive promoter
-
|rowspan="3" |July 26
+
|
-
|align="center"|145
+
|
-
|rowspan="3" align="center"|[[Team:Paris/July 23|July 23]], [[Team:Paris/July 26|July 26]]
+
|align="center"|[[Team:Paris/July 22|July 22]], [[Team:Paris/July 23|July 23]], [[Team:Paris/July 26|July 26]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP104.3
+
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_J23109 J23109]
-
|align="center"|147
+
|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002]
 +
|align="center"|S110
 +
|align="center"|MP102
 +
|align="center"|[[Image:Part_icon_regulatory.png]]<br>Weak Constitutive promoter
 +
|
 +
|
 +
|align="center"|[[Team:Paris/July 22|July 22]], [[Team:Paris/July 26|July 26]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP104.4
+
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_R0040 R0040]
-
|align="center"|51
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-
|- style="background: #dddddd;" 
+
|align="center"|S111
-
| style="background: #D4E2EF;" |MP105.1
+
|align="center"|MP104
-
|rowspan="2" align="center"|[http://partsregistry.org/Part:BBa_S03154 S03154]
+
|align="center"|[[Image:Part_icon_regulatory.png]]<br>pTetR repressible
-
|rowspan="2" align="center" |[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
-
|rowspan="2" align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]
+
-
|rowspan="2" align="center"|B0034(rbs) ->LasI
+
|
|
-
|align="center"|34
 
-
|rowspan="2" align="center"|[[Team:Paris/July 26|July 26]]
 
-
|- style="background: #dddddd;"
 
-
|style="background: #D4E2EF;" |MP105.2
 
|
|
-
|align="center"|31
+
|align="center"|[[Team:Paris/July 23|July 23]], [[Team:Paris/July 26|July 26]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP106.1
+
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_S03154 S03154]
-
|rowspan="2" align="center"|[http://partsregistry.org/Part:BBa_S03879 S03879]
+
|align="center" |[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-
|rowspan="2" align="center" |[http://partsregistry.org/Part:pSB1A2 pSB1A2]  
+
|align="center"|S112
-
|rowspan="2" align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]
+
|align="center"|MP105
-
|rowspan="2" align="center"|B0034(rbs) -> TetR
+
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>B0034(rbs) ->LasI
|
|
-
|align="center"|80
+
|  
-
|rowspan="2" align="center"|[[Team:Paris/August 24|August 24]]
+
|align="center"|[[Team:Paris/July 26|July 26]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP106.3
+
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_S03879 S03879]
 +
|align="center" |[http://partsregistry.org/Part:pSB1A2 pSB1A2]
 +
|align="center"|S113
 +
|align="center"|MP106
 +
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>B0034(rbs) -> TetR
|
|
-
|align="center"|40
+
|
 +
|align="center"|[[Team:Paris/August 24|August 24]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP107
+
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_C0079 C0079]
-
|align="center"|[http://partsregistry.org/Part:BBa_C0079 C0079]
+
|align="center"|[http://partsregistry.org/Part:pSB2K3 pSB2K3]  
|align="center"|[http://partsregistry.org/Part:pSB2K3 pSB2K3]  
-
|align="center"|[[Image:Icon_coding.png]]
+
|align="center"|S114
-
|align="center"|lasR activator with LVA Tag
+
|align="center"|MP107
-
|
+
|align="center"|[[Image:Icon_coding.png]]<br>lasR activator with LVA Tag
|
|
 +
|
|align="center"|[[Team:Paris/July 23|July 23]]
|align="center"|[[Team:Paris/July 23|July 23]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP108
+
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_C0179 C0179]
-
|align="center"|[http://partsregistry.org/Part:BBa_C0179 C0179]
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]  
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]  
-
|align="center"|[[Image:Icon_coding.png]]
+
|align="center"|S115
-
|align="center"|lasR activator
+
|align="center"|MP108
-
| July 26
+
|align="center"|[[Image:Icon_coding.png]]<br>lasR activator
|
|
 +
|
|align="center"|[[Team:Paris/July 22|July 22]], [[Team:Paris/July 23|July 23]], [[Team:Paris/July 26|July 26]]
|align="center"|[[Team:Paris/July 22|July 22]], [[Team:Paris/July 23|July 23]], [[Team:Paris/July 26|July 26]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP109
+
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_E0030 E0030]
-
|align="center"|[http://partsregistry.org/Part:BBa_E0030 E0030]
+
|align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]  
|align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]  
-
|align="center"|[[Image:Part_icon_reporter.png]]
+
|align="center"|S116
-
|align="center"|YFP
+
|align="center"|MP109
-
|
+
|align="center"|[[Image:Part_icon_reporter.png]]<br>YFP
|
|
 +
|align="center"|
|align="center"|[[Team:Paris/July 26|July 26]]
|align="center"|[[Team:Paris/July 26|July 26]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP110
+
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_E0040 E0040]
-
|align="center"|[http://partsregistry.org/Part:BBa_E0040 E0040]
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-
|align="center"|[[Image:Part_icon_reporter.png]]
+
|align="center"|S117
-
|align="center"|GFP
+
|align="center"|MP110
-
|July 26
+
|align="center"|[[Image:Part_icon_reporter.png]]<br>GFP
|
|
 +
|align="center"|
|align="center"|[[Team:Paris/July 23|July 23]], [[Team:Paris/July 26|July 26]]
|align="center"|[[Team:Paris/July 23|July 23]], [[Team:Paris/July 26|July 26]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP111
+
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_E1010 E1010 ]
-
|align="center"|[http://partsregistry.org/Part:BBa_E1010 E1010 ]
+
|align="center"|[http://partsregistry.org/Part:pSB2K3 pSB2K3]
|align="center"|[http://partsregistry.org/Part:pSB2K3 pSB2K3]
-
|align="center"|[[Image:Part_icon_reporter.png]]
+
|align="center"|S118
-
|align="center"|mRFP
+
|align="center"|MP111
-
|July 26
+
|align="center"|[[Image:Part_icon_reporter.png]]<br>mRFP
|
|
 +
|align="center"|
|align="center"|[[Team:Paris/July 23|July 23]], [[Team:Paris/July 26|July 26]]
|align="center"|[[Team:Paris/July 23|July 23]], [[Team:Paris/July 26|July 26]]
 +
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP112
+
| style="background: #D4E2EF;" |OmpR* M57I
-
|align="center"|OmpR M57I
+
|align="center"|Strain
-
|
+
|align="center"|S119
-
|align="center"|[[Image:Icon_coding.png]]
+
|align="center"|
|align="center"|
 +
|align="center"|Strain MG1655<br>[[Image:Icon_coding.png]]<br>OmpR*
|
|
-
|
+
|align="center"|Really mutated?
|align="center"|[[Team:Paris/July 23|July 23]]
|align="center"|[[Team:Paris/July 23|July 23]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP113
+
| style="background: #D4E2EF;" |EnvZ* I281S
-
|align="center"|EnvZ I281S
+
|align="center"|Strain
-
|
+
|align="center"|S120
-
|align="center"|[[Image:Icon_coding.png]]
+
|align="center"|
|align="center"|
 +
|align="center"|Strain MG1655<br>[[Image:Icon_coding.png]]<br>EnvZ*
|
|
|
|
|align="center"|[[Team:Paris/July 23|July 23]]
|align="center"|[[Team:Paris/July 23|July 23]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP114.1
+
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_C0040 C0040]
-
|rowspan="2" align="center"|[http://partsregistry.org/Part:BBa_C0040 C0040]
+
|align="center" |[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-
|rowspan="2" align="center" |[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
|align="center"|S121
-
|rowspan="2" align="center"|[[Image:Icon_coding.png]]
+
|align="center"|MP114
-
|rowspan="2" align="center"|tetR repressor  
+
|align="center"|[[Image:Icon_coding.png]]<br>tetR repressor  
|
|
-
|align="center"|173
+
|  
-
|rowspan="2" align="center"|[[Team:Paris/July 23|July 23]]
+
|align="center"|[[Team:Paris/July 23|July 23]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP114.2
+
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:pSB1A3 pSB1A3]
-
|
+
-
|align="center"|263
+
-
|- style="background: #dddddd;"  
+
-
| style="background: #D4E2EF;" |MP115
+
|align="center"|[http://partsregistry.org/Part:pSB1A3  pSB1A3]  
|align="center"|[http://partsregistry.org/Part:pSB1A3  pSB1A3]  
-
|
+
|align="center"|S122
-
|align="center"|[[Image:Icon_plasmid.png]]
+
|align="center"|MP115
-
|align="center"|
+
|align="center"|[[Image:Icon_plasmid.png]]<br>plasmid
|
|
|
|
|align="center"|[[Team:Paris/July 23|July 23]]
|align="center"|[[Team:Paris/July 23|July 23]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP116
+
|style="background: #D4E2EF;" |L102
-
|align="center"|[http://partsregistry.org/Part:BBa_J23100 pJ23100]
+
-
|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002]
+
-
|align="center"|[[Image:Part_icon_regulatory.png]]
+
-
|align="center"|Strong constitutive promoter in J61002
+
-
|
+
-
|
+
-
|align="center"|[[Team:Paris/July 26|July 26]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |MP117
+
-
|align="center"|[http://partsregistry.org/Part:BBa_J23107 pJ23107]
+
-
|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002]
+
-
|align="center"|[[Image:Part_icon_regulatory.png]]
+
-
|align="center"|Medium constitutive promoter in J61002
+
-
|July 26
+
-
|
+
-
|align="center"|[[Team:Paris/July 24|July 24]], [[Team:Paris/July 26|July 26]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |MP118.1
+
-
|rowspan="2" align="center"|[http://partsregistry.org/Part:BBa_B0015 B0015]
+
-
|rowspan="2" align="center" |[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
+
-
|rowspan="2" align="center"|[[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
-
|rowspan="2" align="center"|Double terminator
+
-
|rowspan="2" |July 26
+
-
|align="center"|220
+
-
|rowspan="2" align="center"|[[Team:Paris/July 24|July 24]], [[Team:Paris/July 26|July 26]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |MP118.3
+
-
|align="center"|187
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |MP119.1
+
-
|rowspan="3" align="center"|[http://partsregistry.org/Part:BBa_I0500 I0500]
+
-
|rowspan="3" align="center"|[http://partsregistry.org/Part:pSB2K3 pSB2K3]
+
-
|rowspan="3" align="center"|[[Image:Part_icon_regulatory.png]]
+
-
|rowspan="3" align="center"|AraC pBAD
+
-
|rowspan="3" |July 26
+
-
|align="center"|42
+
-
|rowspan="3" align="center"|[[Team:Paris/July 24|July 24]], [[Team:Paris/July 26|July 26]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |MP119.2
+
-
|align="center"|26
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |MP119.3
+
-
|align="center"|39
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |MP120
+
-
|align="center"|[http://partsregistry.org/Part:BBa_B0030 B0030]
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-
|align="center"|[[Image:Part_icon_rbs.png]]
+
|align="center"|S123
-
|align="center"|Strong RBS (Efficiency = 0,6)
+
|align="center"|MP124
-
|
+
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]<br>Strong rbs - YFP
-
|
+
|align="center"|D129 (BV) -  D118 (BI)
-
|align="center"|[[Team:Paris/July 26|July 26]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |MP121
+
-
|align="center"|[http://partsregistry.org/Part:BBa_E0422 E0422]
+
-
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
-
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
-
|align="center"|ECFP (RBS+LVA+Double Term)
+
-
|July 26
+
-
|
+
-
|align="center"|[[Team:Paris/July 24|July 24]], [[Team:Paris/July 26|July 26]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |MP122
+
-
|align="center"|[http://partsregistry.org/Part:BBa_E0840 E0840]
+
-
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
-
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
-
|align="center"|gfp tri-part; strong rbs
+
-
|
+
-
|
+
-
|align="center"|[[Team:Paris/July 26|July 26]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |MP123
+
-
|
+
-
|align="center"|[http://partsregistry.org/Part:J61002  J61002]
+
-
|align="center"|[[Image:Icon_plasmid.png]]
+
-
|align="center"|
+
-
|
+
-
|
+
-
|align="center"|[[Team:Paris/August 5| August 5 ]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |MP124
+
-
|align="center"|L102
+
-
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
-
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]
+
-
|align="center"|Strong rbs - YFP<br>D129 (BV) -  D118 (BI)
+
-
|
+
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
 +
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP125
+
| style="background: #D4E2EF;" |L103
-
|align="center"|L103
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]
+
|align="center"|S124
-
|align="center"|Strong rbs - mRFP<br>D129 (BV) -  D122 (BI)
+
|align="center"|MP125
-
|
+
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]<br>Strong rbs - mRFP
 +
|align="center"|D129 (BV) -  D122 (BI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP126
+
| style="background: #D4E2EF;" |L104
-
|align="center"|L104
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]
+
|align="center"|S125
-
|align="center"|Strong rbs - lasR activator<br>D129 (BV) -  D114 (BI)
+
|align="center"|MP126
-
|
+
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>Strong rbs - lasR activator
 +
|align="center"|D129 (BV) -  D114 (BI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP127
+
| style="background: #D4E2EF;" |L105
-
|align="center"|L105
+
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
-
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
|align="center"|S126
-
|align="center"|Strong promoter - ECFP<br>D123 (BV) - D130 (BI)
+
|align="center"|MP127
-
|
+
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Strong promoter - ECFP
 +
|align="center"|D123 (BV) - D130 (BI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
 +
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP128
+
| style="background: #D4E2EF;" |L106
-
|align="center"|L106
+
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
-
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
|align="center"|S127
-
|align="center"|Strong promoter - gfp Tripart<br>D123 (BV) - D131 (BI)
+
|align="center"|MP128
-
|
+
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Strong promoter - GFP Tripart
 +
|align="center"|D123 (BV) - D131 (BI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP129
+
| style="background: #D4E2EF;" |L107
-
|align="center"|L107
+
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
-
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
|align="center"|S128
-
|align="center"|Strong promoter - ECFP<br>D103 (BV) - D130 (BI)
+
|align="center"|MP129
-
|
+
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Strongest promoter - ECFP
 +
|align="center"|D103 (BV) - D130 (BI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
 +
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP130
+
| style="background: #D4E2EF;" |L108
-
|align="center"|L108
+
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
-
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
|align="center"|S129
-
|align="center"|Strong promoter -  gfp Tripart<br>D103 (BV) - D131 (BI)
+
|align="center"|MP130
-
|
+
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Strongest promoter -  GFP Tripart
 +
|align="center"|D103 (BV) - D131 (BI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP131
+
| style="background: #D4E2EF;" |L110
-
|align="center"|L110
+
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
-
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
|align="center"|S130
-
|align="center"|Medium promoter -  gfp Tripart<br>D124 (BV) - D131 (BI)
+
|align="center"|MP131
-
|
+
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Medium promoter -  GFP Tripart
 +
|align="center"|D124 (BV) - D131 (BI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP132
+
| style="background: #D4E2EF;" |L111
-
|align="center"|L111
+
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
-
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
|align="center"|S131
-
|align="center"|Weak promoter - ECFP<br>D104 (BV) - D130 (BI)
+
|align="center"|MP132
-
|
+
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Weak promoter - ECFP
 +
|align="center"|D104 (BV) - D130 (BI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP133
+
| style="background: #D4E2EF;" |L112
-
|align="center"|L112
+
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
-
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
|align="center"|S132
-
|align="center"|Weak promoter - GFP tripart<br>D104 (BV) - D131 (BI)
+
|align="center"|MP133
-
|
+
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Weak promoter - GFP tripart
 +
|align="center"|D104 (BV) - D131 (BI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP134
+
| style="background: #D4E2EF;" |L115
-
|align="center"|L115
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
|align="center"|S133
-
|align="center"|pLas - ECFP<br>D105 (BV) - D130 (BI)
+
|align="center"|MP134
-
|
+
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - ECFP
 +
|align="center"|D105 (BV) - D130 (BI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP135
+
| style="background: #D4E2EF;" |L116
-
|align="center"|L116
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
|align="center"|S134
-
|align="center"|pLas - gfp Tripart<br>D105 (BV) - D131 (BI)
+
|align="center"|MP135
-
|
+
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - gfp Tripart
 +
|align="center"|D105 (BV) - D131 (BI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP136
+
| style="background: #D4E2EF;" |L117
-
|align="center"|L117
+
|align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
|align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
-
|align="center"|[[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
|align="center"|S135
-
|align="center"|yfp - Double Terminator<br>D125 (FV) - D117 (FI)
+
|align="center"|MP136
-
|
+
|align="center"|[[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>YFP - Double Terminator
 +
|align="center"|D125 (FV) - D117 (FI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP137
+
| style="background: #D4E2EF;" |L118
-
|align="center"|L118
+
|align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
|align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
-
|align="center"|[[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
|align="center"|S136
-
|align="center"|rfp - Double Terminator<br>D125 (FV) - D121 (FI)
+
|align="center"|MP137
-
|
+
|align="center"|[[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>mRFP - Double Terminator
 +
|align="center"|D125 (FV) - D121 (FI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP138
+
| style="background: #D4E2EF;" |L119
-
|align="center"|L119
+
|align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
|align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
-
|align="center"|[[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
|align="center"|S137
-
|align="center"|lasR activator + LVA - Double Terminator<br>D125 (FV) - D113 (FI)
+
|align="center"|MP138
-
|
+
|align="center"|[[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>lasR activator + LVA - Double Terminator
 +
|align="center"|D125 (FV) - D113 (FI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP139
+
| style="background: #D4E2EF;" |L121
-
|align="center"|L121
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
|align="center"|S138
-
|align="center"|pTet - gfp tripart<br>D106 (BV) - D131 (BI)
+
|align="center"|MP139
-
|
+
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pTet - GFP  tripart
 +
|align="center"|D106 (BV) - D131 (BI)<br>In Top10 strain
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP140
+
| style="background: #D4E2EF;" |L124
-
|align="center"|L124
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]
+
|align="center"|S139
-
|align="center"|Strongest RBS - rfp<br>D102 (BV) - D122 (BI)
+
|align="center"|MP140
-
|
+
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]<br>Strongest RBS - mRFP
 +
|align="center"|D102 (BV) - D122 (BI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP141
+
| style="background: #D4E2EF;" |L125
-
|align="center"|L125
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]
+
|align="center"|S140
-
|align="center"|Strongest RBS - yfp<br>D102 (BV) - D118 (BI)
+
|align="center"|MP141
-
|
+
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]<br>Strongest RBS - YFP
 +
|align="center"|D102 (BV) - D118 (BI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
 +
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP142
+
| style="background: #D4E2EF;" |MG1655
-
|
+
|align="center"|Strain
-
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
+
|align="center"|S141
-
|align="center"|[[Image:icon_plasmid.png]]
+
|align="center"|
|align="center"|
 +
|align="center"|Strain
 +
|align="center" |
|
|
 +
|align="center" |[[Team:Paris/August 7|Aug 7]]
 +
|- style="background: #dddddd;" 
 +
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_J61002 J61002]
 +
|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002]
 +
|align="center"|S142
 +
|align="center"|MP123
 +
|align="center"|[[Image:Icon_plasmid.png]]<br>plasmid
|
|
-
|align="center"|[[Team:Paris/August 8|August 8]]
+
|
 +
|align="center"|[[Team:Paris/August 5| August 5 ]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP143.1
+
| style="background: #D4E2EF;" |L128
-
|rowspan="2" align="center"|[http://partsregistry.org/Part:BBa_E0240 E0240]
+
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
-
|rowspan="2" align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
|align="center"|S143
-
|rowspan="2" align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
|align="center"|MP144
-
|rowspan="2" align="center"|gfp generator
+
|align="center"|[[Image:Part_icon_regulatory.png]]<br>pFlgA
 +
|align="center"|D132 (FI) -  D136 (FV)
 +
|align="center"|OK sequencing
 +
|align="center"|[[Team:Paris/August 12|August 12]]
 +
|- style="background: #dddddd;" 
 +
| style="background: #D4E2EF;" |L100
 +
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
 +
|align="center"|S146
 +
|align="center"|MP147
 +
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>rbs TetR - ECFP
 +
|align="center"|D110 (BV) -  D130 (BI)
|
|
-
|align="center"|138
+
|align="center"|[[Team:Paris/August 13|August 13]]
-
|rowspan="2" align="center"|[[Team:Paris/August 8|August 8]]
+
 
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP143.2
+
| style="background: #D4E2EF;" |L114
 +
|align="center" |[http://partsregistry.org/Part:pSB2K3 pSB2K3]
 +
|align="center"|S148
 +
|align="center"|MP149
 +
|align="center"| [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>araC/pBad - GFP tripart
 +
|align="center"|D126 (BV) - D131 (BI)
|
|
-
|align="center"|150
+
|align="center"|[[Team:Paris/August 13|August 13]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP144.1
+
| style="background: #D4E2EF;" |L120
-
|rowspan="4" align="center"|L128
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-
|rowspan="4" align="center"|[http://partsregistry.org/Part:J61002 J61002]
+
|align="center"|S149
-
|rowspan="4" align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
+
|align="center"|MP150
-
|rowspan="4" align="center"|pFlgA-RFP<br>D132 (FI) - D136 (FV)
+
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pTet - ECFP
-
|rowspan="4" align="center"|MP144.3 ; MP144.4
+
|align="center"|D106 (BV) - D130 (BI)
-
|align="center"|127
+
|
-
|rowspan="4" align="center"|[[Team:Paris/August 12|August 12]]
+
|align="center"|[[Team:Paris/August 13|August 13]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP144.2
+
| style="background: #D4E2EF;" |L122
-
|align="center"|166
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
 +
|align="center"|S150
 +
|align="center"|MP151
 +
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS-lasI - ECFP
 +
|align="center"|D107 (BV) - D130 (BI)
 +
|
 +
|align="center"|[[Team:Paris/August 13|August 13]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP144.3
+
| style="background: #D4E2EF;" |L123
-
|align="center"|163
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
 +
|align="center"|S151
 +
|align="center"|MP152
 +
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS lasI - GFP tripart
 +
|align="center"|D107 (BV) - D131 (BI)
 +
|
 +
|align="center"|[[Team:Paris/August 13|August 13]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP144.4
+
| style="background: #D4E2EF;" |L126
-
|align="center"|154
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
 +
|align="center"|S152
 +
|align="center"|MP153
 +
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>Strongest RBS (1)- LasR activator (+LVA)
 +
|align="center"|D102 (BV) - D114 (BI)
 +
|
 +
|align="center"|[[Team:Paris/August 13|August 13]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP145.1
+
| style="background: #D4E2EF;" |L132
-
|rowspan ="4" align="center"|L129
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-
|rowspan="4" align="center"|[http://partsregistry.org/Part:J61002 J61002]
+
|align="center"|S153
-
|rowspan ="4" align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
+
|align="center"|MP154
-
|rowspan ="4" align="center"|pFlgB-RFP<br>D133 (FI) - D136 (FV)
+
|align="center"|[[Image:Icon_coding.png]]<br>flhDC
-
|rowspan ="4" align="center"|MP145.6 ; MP145.7
+
|align="center"|D142(FV) - D139(FI)  
-
|align="center"|136
+
|
-
|rowspan ="4" align="center"|[[Team:Paris/August 12|August 12]]
+
|align="center"|[[Team:Paris/August 13|August 13]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP145.2
+
| style="background: #D4E2EF;" |L133
-
|align="center"|151
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
 +
|align="center"|S154
 +
|align="center"|MP155
 +
|align="center"|[[Image:Icon_coding.png]]<BR>OmpR*
 +
|align="center"|D142(FV) - D140(FI)
 +
|align="center"|WT sequence
 +
|align="center"| [[Team:Paris/August 19|August 19]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP145.6
+
| style="background: #D4E2EF;" |L134
-
|align="center"|163
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
 +
|align="center"|S155
 +
|align="center"|MP156
 +
|align="center"|[[Image:Icon_coding.png]]<BR> EnvZ*
 +
|align="center"|D142(FV) - D141(FI)
 +
|align="center"|lacks about 300 bp in EnvZ*
 +
|align="center"|[[Team:Paris/August 19| August 19]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP145.7
+
| style="background: #D4E2EF;" |L138
-
|align="center"|188
+
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
 +
|align="center"|S156
 +
|align="center"|MP157
 +
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>GFP generator (E0240)
 +
|align="center"| D137(FV) - D138(FI)
 +
|
 +
|align="center"|[[Team:Paris/August 13|August 13]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP146.1
+
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_E0240 E0240]
-
|rowspan ="4" align="center"|L130
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-
|rowspan="4" align="center"|[http://partsregistry.org/Part:J61002 J61002]
+
|align="center"|S157
-
|rowspan ="4" align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
+
|align="center"|MP143
-
|rowspan ="4" align="center"|pFlhB-RFP<br>D134 (FI) -  D136 (FV)
+
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>GFP generator
-
|rowspan ="4" align="center"|MP146.1 ; MP146.2
+
|align="center"|
-
|align="center"|298
+
|align="center"|
-
|rowspan="4" align="center"|[[Team:Paris/August 12|August 12]]
+
|align="center"|[[Team:Paris/August 15|August 15]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP146.2
+
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:pSB3K3 pSB3K3]
-
|align="center"|142
+
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
 +
|align="center"|S158
 +
|align="center"|MP142
 +
|align="center"|[[Image:Icon_plasmid.png]]<br>plasmid
 +
|
 +
|align="center"|low to medium copy plasmid with p15A ORI
 +
|align="center"|[[Team:Paris/August 15|August 15]]
 +
 
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP146.7
+
| style="background: #D4E2EF;" |L141
-
|align="center"|170
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
 +
|align="center"|S160
 +
|align="center"|MP159
 +
|align="center"|[[Image:Icon_coding.png]]<br>flhC
 +
|align="center"|D142 (ES) - D145 (ES)
 +
|align="center"|has not been sequenced
 +
|align="center"|[[Team:Paris/August 15|August 15]]
 +
 
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP146.8
+
| style="background: #D4E2EF;" |L151
-
|align="center"|143
+
|align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
 +
|align="center"|S162
 +
|align="center"|MP162
 +
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>rbs-lasR + dbl ter
 +
|align="center"| D147 (FI) - D125 (FV)
 +
|
 +
|align="center"|[[Team:Paris/August 19|August 19]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP147
+
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_B0032 B0032]
-
|align="center"|L100
+
| align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
|align="center"|S163
-
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
|align="center"|MP163
-
|align="center"|rbs TetR - ECFP<br>D110 (BV) -  D130 (BI)
+
|align="center"|[[Image:Icon_rbs.png]]<br> medium rbs (efficiency = 0,3)
-
|
+
|
|
-
|align="center"|[[Team:Paris/August 13|August 13]]
+
|
 +
|align="center"|[[Team:Paris/August 20|August 20]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #FF0033;" |MP148.1
+
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_E0422 E0422]
-
|rowspan="3" align="center"|L101
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-
|rowspan="3" align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
|align="center"|S164
-
|rowspan="3" align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
|align="center"|MP121<br>MP164
-
|rowspan="3" align="center"|rbs TetR - GFP tripart<br>D110 (BV) -  D131 (BI) DO NOT WORK !!!!
+
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS+ ECFP+ LVA+ term
-
|rowspan="3" align="center"|MP148.2 ; MP148.3
+
|
-
|align="center"|109
+
|align="center"|
-
|rowspan="3" align="center"|[[Team:Paris/August 13|August 13]]
+
|align="center"|[[Team:Paris/July 24|July 24]], [[Team:Paris/July 26|July 26]]<br>[[Team:Paris/August 20|August 20]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #FF0033;" |MP148.2
+
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_E0430 E0430]
-
|align="center"|123
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
 +
|align="center"|S165
 +
|align="center"|MP165
 +
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS+ YFP+ LVA- term
 +
|
 +
|align="center"|
 +
|align="center"|[[Team:Paris/August 20|August 20]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #FF0033;" |MP148.3
+
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_E0432 E0432]
-
|align="center"|123
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
 +
|align="center"|S166
 +
|align="center"|MP166
 +
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS+ YFP LVA+ term 
 +
|align="center"|
 +
|align="center"|BUT be careful , it doesn't work. See registry
 +
|align="center"|[[Team:Paris/August 20|August 20]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP149
+
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_E0420 E0420]
-
|align="center"|L114
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-
|align="center" |[http://partsregistry.org/Part:pSB2K3 pSB2K3]
+
|align="center"|S167
-
|align="center"| [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
|align="center"|MP167
-
|align="center"|AracpBAD - gfp tripart<br>D126 (BV) - D131 (BI)
+
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS+ ECFP LVA- term
 +
|
 +
|align="center"|
 +
|align="center"|[[Team:Paris/August 20|August 20]]
 +
|- style="background: #dddddd;" 
 +
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_I732078 I732078]
 +
|align="center"|[http://partsregistry.org/Part:pSB1A3 pSB1A3]
 +
|align="center"|S168
 +
|align="center"|MP168
 +
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS+ mRFP LVA+ term
 +
|
 +
|align="center"|
 +
|align="center"|[[Team:Paris/August 20|August 20]]
 +
|- style="background: #dddddd;" 
 +
| style="background: #D4E2EF;" |L153
 +
|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002]
 +
|align="center"|S169
 +
|align="center"|MP169
 +
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> Strong promoter-rbs-lasR-dble ter
 +
|align="center"|D123 (BV) - D165 (BI)
|
|
 +
|align="center"|[[Team:Paris/August 20|August 20]]
 +
|- style="background: #dddddd;" 
 +
| style="background: #D4E2EF;" |L154
 +
|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002]
 +
|align="center"|S170
 +
|align="center"|MP170
 +
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> strongest promoter-rbs-lasR-dble ter
 +
|align="center"|D103 (BV) - D165 (BI)
|
|
-
|align="center"|[[Team:Paris/August 13|August 13]]
+
|align="center"|[[Team:Paris/August 20|August 20]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP150
+
| style="background: #D4E2EF;" |L121
-
|align="center"|L120
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
|align="center"|S171
-
|align="center"|tetR repressible promoter - ECFP<br>D106 (BV) - D130 (BI)
+
|align="center"|not done
-
|
+
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pTet - gfp Tripart
-
|
+
|align="center"|D106 (BV) - D131 (BI)
-
|align="center"|[[Team:Paris/August 13|August 13]]
+
|align="center"|Same as S138 but in chemically labmade competent MG1655 bacteria. For study of doubling time of  population.
 +
|align="center" |[[Team:Paris/August 22|Aug 22]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP151
+
| style="background: #D4E2EF;" |L167
-
|align="center"|L122
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
|align="center"|S172
-
|align="center"|RBS-lasI - ECFP <br>D107 (BV) - D130 (BI)
+
|align="center"|MP171
 +
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_regulatory.png]]<br>gfp generator(E0240) - pTet
|
|
-
|
+
|  
-
|align="center"|[[Team:Paris/August 13|August 13]]
+
|align="center"|[[Team:Paris/August 25|August 25]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP152
+
| style="background: #D4E2EF;" |L166
-
|align="center"|L123
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-
|align="center"| [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
|align="center"|S173
-
|align="center"|RBS lasI - gfp tripart<br>D107 (BV) - D131 (BI)
+
|align="center"|MP172
 +
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>RBS(B0032) - tetR
|
|
 +
|
 +
|align="center"|[[Team:Paris/August 25|August 25]]
 +
|- style="background: #dddddd;" 
 +
| style="background: #D4E2EF;" |L160
 +
|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002]
 +
|align="center"|S174
 +
|align="center"|MP173
 +
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)
 +
|align="center"|D166 (FV) - D132 (FI)
 +
|align="center"|Fluorescence OK
 +
|align="center"|[[Team:Paris/August 22|August 22]]
 +
|- style="background: #dddddd;" 
 +
| style="background: #D4E2EF;" |L161
 +
|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002]
 +
|align="center"|S175
 +
|align="center"|MP174
 +
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)
 +
|align="center"|D167 (FV) - D132 (FI)
|
|
-
|align="center"|[[Team:Paris/August 13|August 13]]
+
|align="center"|[[Team:Paris/August 22|August 22]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP153.1
+
| style="background: #D4E2EF;" |pFlhB
-
|rowspan="3" align="center"|L126
+
|align="center"|pCR4Blunt-TOPO
-
|rowspan="3" align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
|align="center"|S178
-
|rowspan="3" align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]
+
|align="center"|MP175
-
|rowspan="3" align="center"|Strongest RBS (1)- LasR activator (+LVA)<br>D102 (BV) - D114 (BI)
+
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlhB-RBS (WT)
-
|rowspan="3" align="center"|MP153.1 ; MP153.3
+
|  
-
|align="center"|100
+
|align="center"|OK sequencing
-
|rowspan="3" align="center"|[[Team:Paris/August 13|August 13]]
+
|align="center"|[[Team:Paris/September 04|September 04]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP153.2
+
| style="background: #D4E2EF;" |L
-
|align="center"|131
+
|align="center"|pCR4Blunt-TOPO
 +
|align="center"|S179
 +
|align="center"|MP176
 +
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFliA-RBS (WT)
 +
|align="center"|
 +
|align="center"|pFliA with conserved Pst1 site
 +
|align="center"|[[Team:Paris/September 04|September 04]]
 +
 
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP153.3
+
| style="background: #D4E2EF;" |L
-
|align="center"|113
+
|align="center"|pCR4Blunt-TOPO
 +
|align="center"|S181
 +
|align="center"|MP178
 +
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFliL-RBS (WT)
 +
|align="center"|
 +
|align="center"|OK sequencing
 +
|align="center"|[[Team:Paris/September 04|September 04]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #FF0033;" |MP154
+
| style="background: #D4E2EF;" |L
-
|align="center"|L132
+
|align="center"|pCR4Blunt-TOPO
-
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
|align="center"|S180
-
|align="center"|[[Image:Icon_coding.png]]
+
|align="center"|MP177
-
|align="center"|flhDC <br> D142(FV) - D139(FI)  
+
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlhDC-RBS (WT)
 +
|
 +
|align="center"|OK sequencing
 +
|align="center"|[[Team:Paris/September 04|September 04]]
 +
|- style="background: #dddddd;" 
 +
| style="background: #D4E2EF;" |L
 +
|align="center"|pCR4Blunt-TOPO
 +
|align="center"|S182
 +
|align="center"|MP179
 +
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>RBS(FlhDC) - FlhDC
 +
|
 +
|align="center"|FlhDC with conserved Pst1 site
 +
|align="center"|[[Team:Paris/September 04|September 04]]
 +
|- style="background: #dddddd;" 
 +
| style="background: #D4E2EF;" |L
 +
|align="center"|pCR4Blunt-TOPO
 +
|align="center"|S183
 +
|align="center"|MP180
 +
|align="center"|[[Image:Icon_coding.png]]<br> FliA2
 +
|align="center"|
 +
|align="center"|mutated at EcoR1 and Pst1 internal site
 +
|align="center"|[[Team:Paris/September 04|September 04]]
 +
|- style="background: #dddddd;" 
 +
| style="background: #D4E2EF;" |L
 +
|align="center"|pCR4Blunt-TOPO
 +
|align="center"|S184
 +
|align="center"|MP181
 +
|align="center"|[[Image:Icon_coding.png]]<br> FliA3
 +
|align="center"|
 +
|align="center"|FliA mutated at EcoR1 and Pst1 site
 +
|align="center"|[[Team:Paris/September 04|September 04]]
 +
|- style="background: #dddddd;" 
 +
| style="background: #D4E2EF;" |L
 +
|align="center"|pCR4Blunt-TOPO
 +
|align="center"|S185
 +
|align="center"|MP182
 +
|align="center"|[[Image:Icon_coding.png]]<br> EnvZ*
 +
|align="center"|
 +
|align="center"|the good one but not mutated for EcoR1 site
 +
|align="center"|[[Team:Paris/September 04|September 04]]
 +
|- style="background: #dddddd;" 
 +
| style="background: #D4E2EF;" |L
 +
|align="center"|pCR4Blunt-TOPO
 +
|align="center"|S186
 +
|align="center"|MP183
 +
|align="center"|[[Image:Icon_coding.png]]<br> OmpR WT
 +
|align="center"|
 +
|align="center"|OmpR WT not the mutated
 +
|align="center"|[[Team:Paris/September 04|September 04]]
 +
|- style="background: #dddddd;" 
 +
| style="background: #D4E2EF;" |pFlgB
 +
|align="center"|pCR4Blunt-TOPO
 +
|align="center"|S187
 +
|align="center"|MP184
 +
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlgB-RBS (WT)
 +
|
 +
|align="center"|OK sequencing
 +
|align="center"|[[Team:Paris/September 04|September 04]]
 +
|- style="background: #dddddd;" 
 +
| style="background: #D4E2EF;" |L
 +
|align="center"|pCR4Blunt-TOPO
 +
|align="center"|S188
 +
|align="center"|MP185
 +
|align="center"|[[Image:Icon_coding.png]]<br> OmpR WT
 +
|align="center"|
 +
|align="center"|OmpR WT not the mutated
 +
|align="center"|[[Team:Paris/September 04|September 04]]
 +
|- style="background: #dddddd;" 
 +
| style="background: #D4E2EF;" |L179
 +
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
 +
|align="center"|S200
 +
|align="center"|MP200
 +
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>J23101 + RBS (B0032) + tet R
|
|
|
|
-
|align="center"|[[Team:Paris/August 13|August 13]]
+
|align="center"|[[Team:Paris/September 24|September 24]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #FF0033;" |MP155
+
| style="background: #D4E2EF;" |L
-
|align="center"|L133
+
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
-
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
|align="center"|S201
-
|align="center"|[[Image:Icon_coding.png]]
+
|align="center"|MP201
-
|align="center"|OmpR*<br> D142(FV) - D140(FI)
+
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlgA(RBS WT)-EnvZ*
-
|WT sequence (August 19)
+
|
|
-
|align="center"|[[Team:Paris/August 13|August 13]] ; [[Team:Paris/August 19|August 19]]
+
|align="center"|probably wrong according to MP208 and MP209 that have been sequenced
 +
|align="center"|[[Team:Paris/September 24|September 24]]
 +
 
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #FF0033;" |MP156
+
| style="background: #D4E2EF;" |L198
-
|align="center"|L134
+
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
-
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
|align="center"|S207
-
|align="center"|[[Image:Icon_coding.png]]
+
|align="center"|MP207
-
|align="center"| EnvZ*<br> D142(FV) - D141(FI)
+
|align="center"|[[Image:Icon_regulatory.png]]<br>pFliA
-
|lacks about 300 bp in EnvZ* (August 19)
+
|
|
-
|align="center"|[[Team:Paris/August 13| August 13]] [[Team:Paris/August 19| August 19]]
+
|align="center"|OK sequencing
 +
|align="center"|[[Team:Paris/October 04|October 04]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP157
+
| style="background: #D4E2EF;" |L203
-
|align="center"|L138
+
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
-
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
|align="center"|S208
-
|align="center"|gfp generator (E0240)<br> D137(FV) - D138(FI)
+
|align="center"|MP208
 +
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br> pFlgA(RBS WT)-EnvZ*
|
|
 +
|align="center"|in fact lack of pFlgA
 +
|align="center"|[[Team:Paris/October 04|October 04]]
 +
|- style="background: #dddddd;" 
 +
| style="background: #D4E2EF;" |L206
 +
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
 +
|align="center"|S209
 +
|align="center"|MP209
 +
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-EnvZ*-mRFP tripart LVA+
|
|
-
|align="center"|[[Team:Paris/August 13|August 13]]
+
|align="center"|in fact lack of pFlgA
 +
|align="center"|[[Team:Paris/October 04|October 04]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP158
+
| style="background: #D4E2EF;" |L209
-
|align="center"|L139
+
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
 +
|align="center"|S210
 +
|align="center"|MP210
 +
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFliL(RBS WT)
 +
|
 +
|align="center"|OK sequencing
 +
|align="center"|[[Team:Paris/October 10|October 10]]
 +
|- style="background: #dddddd;" 
 +
| style="background: #D4E2EF;" |L
 +
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
 +
|align="center"|S211
 +
|align="center"|MP211
 +
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlgA(RBS WT)
 +
|
 +
|align="center"|OK sequencing
 +
|align="center"|[[Team:Paris/October 10|October 10]]
 +
|- style="background: #dddddd;" 
 +
| style="background: #D4E2EF;" |L208
 +
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
 +
|align="center"|S212
 +
|align="center"|MP212
 +
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlhB(RBS WT)
 +
|
 +
|align="center"|OK sequencing
 +
|align="center"|[[Team:Paris/October 10|October 10]]
 +
|- style="background: #dddddd;" 
 +
| style="background: #D4E2EF;" |L197
 +
|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002]
 +
|align="center"|S213
 +
|align="center"|MP213
 +
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlhB(RBS WT)
 +
|
 +
|align="center"|OK sequencing
 +
|align="center"|[[Team:Paris/October 10|October 10]]
 +
|- style="background: #dddddd;" 
 +
| style="background: #D4E2EF;" |L200
 +
|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002]
 +
|align="center"|S214
 +
|align="center"|MP214
 +
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFliL(RBS WT)
 +
|
 +
|align="center"|OK sequencing
 +
|align="center"|[[Team:Paris/October 10|October 10]]
 +
 +
|- style="background: #dddddd;" 
 +
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:pSB4T5 pSB4T5]
 +
|align="center"|[http://partsregistry.org/Part:pSB4T5 pSB4T5]
 +
|align="center"|S215
 +
|align="center"|MP215
 +
|align="center"|[[Image:icon_plasmid.png]]<br>plasmid
 +
|
 +
|align="center"|low copy plasmid with pSC101 ORI
 +
|align="center"|[[Team:Paris/October 14|October 14]]
 +
|- style="background: #dddddd;" 
 +
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_E0434 E0434]
 +
|align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
 +
|align="center"|S216
 +
|align="center"|MP216
 +
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>YFP tripart +AAV
 +
|
 +
|align="center"|Fluorescence OK
 +
|align="center"|[[Team:Paris/October 15|October 15]]
 +
|- style="background: #dddddd;" 
 +
| style="background: #D4E2EF;" |JM45
 +
|align="center"|pJM45
 +
|align="center"|S217
 +
|align="center"|MP217
 +
|align="center"|[[Image:Icon_plasmid.png]]<br>plasmid
 +
|
 +
|align="center"|inducible FlhDC in pBad18 (ORI pBR / ampicillin resistance)
 +
|align="center"|[[Team:Paris/October 24|October 24]]
 +
|- style="background: #dddddd;" 
 +
| style="background: #D4E2EF;" |L226
 +
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
 +
|align="center"|S218
 +
|align="center"|MP218
 +
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(RBS WT)-EnvZ*
 +
|
|align="center"|
|align="center"|
-
|align="center"|E0240 RBS+<br> D140 (PstI) - D144 (ES)
+
|align="center"|[[Team:Paris/October 24|October 24]]
-
|seems to have the plasmid without the insert and one with the insert
+
 
 +
|- style="background: #dddddd;" 
 +
| style="background: #D4E2EF;" |L227
 +
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
 +
|align="center"|S219
 +
|align="center"|MP219
 +
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP
|
|
-
|align="center"|[[Team:Paris/August 15|August 15]]
+
|align="center"|
 +
|align="center"|[[Team:Paris/October 24|October 24]]
 +
 
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP159
+
| style="background: #D4E2EF;" |L228
-
|align="center"|L141
+
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
-
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
|align="center"|S220
 +
|align="center"|MP220
 +
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (+LVA)
 +
|
|align="center"|
|align="center"|
-
|align="center"|flhD<br> D142 (ES) - D145 (ES)
+
|align="center"|[[Team:Paris/October 24|October 24]]
-
|has not been sequenced
+
 
 +
|- style="background: #dddddd;" 
 +
| style="background: #D4E2EF;" |L229
 +
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
 +
|align="center"|S221
 +
|align="center"|MP221
 +
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP
|
|
-
|align="center"|[[Team:Paris/August 15|August 15]]
+
|align="center"|
 +
|align="center"|[[Team:Paris/October 24|October 24]]
 +
 
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP160
+
| style="background: #D4E2EF;" |L232
-
|align="center"|L142
+
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
-
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
|align="center"|S222
 +
|align="center"|MP222
 +
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (+AAV)
 +
|
|align="center"|
|align="center"|
-
|align="center"|flhDC+promotor<br> D143 (ES) - D145 (ES)
+
|align="center"|[[Team:Paris/October 24|October 24]]
-
|does not have the insert
+
 
 +
|- style="background: #dddddd;" 
 +
| style="background: #D4E2EF;" |L234
 +
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
 +
|align="center"|S223
 +
|align="center"|MP223
 +
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)
|
|
-
|align="center"|[[Team:Paris/August 15|August 15]]
+
|align="center"|
 +
|align="center"|[[Team:Paris/October 24|October 24]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP161
+
| style="background: #D4E2EF;" |L225
-
|align="center"|L150
+
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
-
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
|align="center"|S224
-
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
|align="center"|MP224
-
|align="center"|pLas + rbs-tetR-gfp tripart<br> D105 (SP) - D146 (XP)
+
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFliL(RBS WT)-EnvZ*
-
|incorrect clone -> to do again
+
|
|
-
|align="center"|[[Team:Paris/August 19|August 19]]
+
|align="center"|
 +
|align="center"|[[Team:Paris/October 26|October 26]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP162.1
+
| style="background: #D4E2EF;" |L233
-
|rowspan="3" align="center"|L151.1
+
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
-
|rowspan="3" align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
+
|align="center"|S225
-
|rowspan="3" align="center"|[[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
|align="center"|MP225
-
|rowspan="3" align="center"|rbs-lasR + double terminator<br> D147 (ES) - D125 (EX)
+
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlhB(RBS WT)-GFP
|
|
-
|align="center"|151
+
|align="center"|
-
|rowspan="3" align="center"|[[Team:Paris/August 19|August 19]]
+
|align="center"|[[Team:Paris/October 26|October 26]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP162.2
+
| style="background: #D4E2EF;" |U317
 +
|align="center"|pU317
 +
|align="center"|S226
 +
|align="center"|MP226
 +
|align="center"|[[Image:Icon_plasmid.png]]<br>plasmid
|
|
-
|align="center"|172
+
|align="center"|inducible FliA in pBad18 (ORI pBR  / ampicillin resistance)
 +
|align="center"|[[Team:Paris/October 23|October 23]]
 +
 
 +
 
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP162.3
+
| style="background: #D4E2EF;" |NEC283
 +
|align="center"|Strain
 +
|align="center"|S227
 +
|align="center"|
 +
|align="center"|Strain MG1655
|
|
-
|align="center"|149
+
|align="center"|Δ FliA Frt
 +
|align="center"|[[Team:Paris/October 25|October 25]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP163.1
+
| style="background: #D4E2EF;" |NEC284
-
|rowspan="2" align="center"|[http://partsregistry.org/Part:BBa_B0032 B0032]
+
|align="center"|Strain
-
|rowspan="2" align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
|align="center"|S228
-
|rowspan="2" align="center"|[[Image:Icon_rbs.png]]
+
|align="center"|
-
|rowspan="2" align="center"|RBS
+
|align="center"|Strain MG1655
|
|
-
|align="center"|80
+
|align="center"|Δ FlhD Frt
-
|rowspan="2" align="center"|[[Team:Paris/August 20|August 20]]
+
|align="center"|[[Team:Paris/October 25|October 25]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP163.2
+
| style="background: #D4E2EF;" |NEC285
 +
|align="center"|Strain
 +
|align="center"|S229
 +
|align="center"|
 +
|align="center"|Strain MG1655
|
|
-
|align="center"|79
+
|align="center"|Δ FlgM Frt
 +
|align="center"|[[Team:Paris/October 25|October 25]]
 +
|- style="background: #dddddd;"
 +
| style="background: #D4E2EF;" |Δ EnvZ :: Cm
 +
|align="center"|Strain
 +
|align="center"|S230
 +
|align="center"|
 +
|align="center"|Strain MG1655
 +
|
 +
|align="center"|Δ EnvZ :: Cm
 +
|align="center"|[[Team:Paris/October 25|October 25]]
 +
|- style="background: #dddddd;"
 +
| style="background: #D4E2EF;" |L216
 +
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
 +
|align="center"|MP231
 +
|align="center"|S231
 +
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*
 +
|
 +
|align="center"|inducible EnvZ*
 +
|align="center"|[[Team:Paris/October 27|October 27]]
 +
|- style="background: #dddddd;"
 +
| style="background: #D4E2EF;" |L236
 +
|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002 ]
 +
|align="center"|MP232
 +
|align="center"|S232
 +
|align="center"|[[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+WT RBS)-EnvZ*-GFP generator
 +
|
 +
|align="center"|
 +
|align="center"|[[Team:Paris/October 29|October 29]]
 +
|- style="background: #dddddd;"
 +
| style="background: #D4E2EF;" |L235
 +
|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002 ]
 +
|align="center"|MP233
 +
|align="center"|S233
 +
|align="center"|[[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+WT RBS)-EnvZ*-GFP generator
 +
|
 +
|align="center"|
 +
|align="center"|[[Team:Paris/October 29|October 29]]
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP164
+
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_J23100 J23100]
-
|align="center"|[http://partsregistry.org/Part:BBa_E0422 E0422]
+
|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002]  
-
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
|align="center"|MP1-2007
-
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
|align="center"|MP116
-
|align="center"|RBS+ ECFP+ LVA+ term
+
|align="center"|[[Image:Part_icon_regulatory.png]]<br>Strong constitutive promoter
|
|
|
|
-
|align="center"|[[Team:Paris/August 20|August 20]]
+
|align="center"|[[Team:Paris/July 26|July 26]]
 +
 
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP165
+
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_J23107 J23107]
-
|align="center"|[http://partsregistry.org/Part:BBa_E0430 E0430]
+
|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002]
-
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
|align="center"|MP2-2007
-
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
|align="center"|MP117
-
|align="center"|RBS+ YFP+ LVA- term
+
|align="center"|[[Image:Part_icon_regulatory.png]]<br>Medium constitutive promoter
|
|
-
|  
+
|
-
|align="center"|[[Team:Paris/August 20|August 20]]
+
|align="center"|[[Team:Paris/July 24|July 24]], [[Team:Paris/July 26|July 26]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP166
+
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_B0030 B0030]
-
|align="center"|[http://partsregistry.org/Part:BBa_E0432 E0432]
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
|align="center"|MP5-2007
-
|align="center"|RBS+ YFP LVA+ term  BUT be careful , it doesn't work. See registry
+
|align="center"|MP120
 +
|align="center"|[[Image:Part_icon_rbs.png]]<br>Strong RBS (Efficiency = 0,6)
|
|
|  
|  
-
|align="center"|[[Team:Paris/August 20|August 20]]
+
|align="center"|[[Team:Paris/July 26|July 26]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP167
+
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_B0015 B0015]
-
|align="center"|[http://partsregistry.org/Part:BBa_E0420 E0420]
+
|align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
-
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
|align="center"|MP3-2007
-
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
|align="center"|MP118
-
|align="center"|RBS+ ECFP LVA- term ceci ne contient pas de LVA apparemment. ça correspondrait au ECFP normal de longue durée de vie
+
|align="center"|[[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Double terminator
|
|
 +
|align="center"|
 +
|align="center"|[[Team:Paris/July 24|July 24]], [[Team:Paris/July 26|July 26]]
 +
 +
 +
|- style="background: #dddddd;" 
 +
| style="background: #D4E2EF;" |L174
 +
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
 +
|align="center"|S
 +
|align="center"|MP202
 +
|align="center"|<br>[[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>Strong constitutive promoter-GFP generator
 +
|align="center"|J23101-E0240
|  
|  
-
|align="center"|[[Team:Paris/August 20|August 20]]
+
|align="center"|[[Team:Paris/September 26|September 26]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP168
+
| style="background: #D4E2EF;" |L157
-
|align="center"|[http://partsregistry.org/Part:BBa_I732078 I732078]
+
|align="center"|[http://partsregistry.org/Part:pSB1K3 pSB1AK3]
-
|align="center"|[http://partsregistry.org/Part:pSB1A3 pSB1A3]
+
|align="center"|
-
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
|align="center"|
-
|align="center"|RBS+ mRFP LVA+ term
+
|align="center"|[[Image:Icon_coding.png]][[Image:Icon_rbs.png]]<br> tetR-rbs (B0015)
 +
|align="center"|D125 (BV) - D162 (BI)
|
|
-
|
+
|align="center"|[[Team:Paris/August 23|August 23]]
-
|align="center"|[[Team:Paris/August 20|August 20]]
+
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP171
+
| style="background: #D4E2EF;" |L155
-
|align="center"|L167
+
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
-
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
|align="center"|
-
|align="center"|gfp generator(E0240) - pTet<br>[[Image:Part_icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_regulatory.png]]
+
|align="center"|
-
|align="center"|gfp generator(E0240) - pTet
+
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> strongest promoter-gfp generator
 +
|align="center"|D164(FV) - D163 (FI)
|
|
-
|
+
|align="center"|[[Team:Paris/August 22|August 22]]
-
|align="center"|[[Team:Paris/August 25|August 25]]
+
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |MP172
+
| style="background: #D4E2EF;" |L156
-
|align="center"|L166
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-
|align="center"|RBS(B0032) - tetR<br>[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]
+
|align="center"|
-
|align="center"|RBS(B0032) - tetR
+
|align="center"|
 +
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pTet-gfp generator
 +
|align="center"|D161 (FV) - D163 (FI)
|
|
-
|
+
|align="center"|[[Team:Paris/August 22|August 22]]
-
|align="center"|[[Team:Paris/August 25|August 25]]
+
|}
|}
Line 1,127: Line 1,450:
|width=5%| Enzyme 2
|width=5%| Enzyme 2
|width=5%| Obs
|width=5%| Obs
-
|width=5%| Size Matrix
+
|width=5%| Size Matrix (bp)
-
|width=5%| Size BB  
+
|width=5%| Size BB (bp)
|width=20%| Description
|width=20%| Description
|width=5%| Product in use
|width=5%| Product in use
Line 1,948: Line 2,271:
|style="background: #C5D0EA;" |D164
|style="background: #C5D0EA;" |D164
|align="center" |MP 101 - pJ23101  
|align="center" |MP 101 - pJ23101  
-
|align="center" |[http://partsregistry.org/Part:BBa_pJ61002 pJ61002]
+
|align="center" |[http://partsregistry.org/Part:BBa_J61002 J61002]
|align="center" |SpeI
|align="center" |SpeI
|align="center" |PstI
|align="center" |PstI
Line 1,979: Line 2,302:
|align="center" |FV
|align="center" |FV
|align="center" |-
|align="center" |-
-
|align="center" |2942 bp
+
|align="center" |2942  
|align="center" |RBS (B0034)+ YFP+ LVA- term B0015<br>[[Image:Part_icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
|align="center" |RBS (B0034)+ YFP+ LVA- term B0015<br>[[Image:Part_icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
|align="center" |
|align="center" |
Line 1,986: Line 2,309:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
|style="background: #C5D0EA;" |D179
|style="background: #C5D0EA;" |D179
-
|align="center" |MP3.4
+
|align="center" |MP3.4 or MP118
|align="center" |[http://partsregistry.org/Part:BBa_pSB1AK3 pSB1AK3]
|align="center" |[http://partsregistry.org/Part:BBa_pSB1AK3 pSB1AK3]
|align="center" |SpeI
|align="center" |SpeI
Line 2,000: Line 2,323:
|style="background: #C5D0EA;" |D180
|style="background: #C5D0EA;" |D180
|align="center" |MP101.1
|align="center" |MP101.1
-
|align="center" |[http://partsregistry.org/Part:BBa_pJ61002 pJ61002]
+
|align="center" |[http://partsregistry.org/Part:BBa_J61002 J61002]
|align="center" |SpeI
|align="center" |SpeI
|align="center" |PstI
|align="center" |PstI
Line 2,096: Line 2,419:
|align="center" |Insert
|align="center" |Insert
|align="center" |-
|align="center" |-
-
|align="center" |767 bp
+
|align="center" |767  
|align="center" |OmpR*<br>[[Image:Icon_coding.png]]
|align="center" |OmpR*<br>[[Image:Icon_coding.png]]
|align="center" |
|align="center" |
Line 2,104: Line 2,427:
|style="background: #C5D0EA;"|D189
|style="background: #C5D0EA;"|D189
|align="center" |MP 101.2
|align="center" |MP 101.2
-
|align="center" |[http://partsregistry.org/Part:BBa_pJ61002 pJ61002]
+
|align="center" |[http://partsregistry.org/Part:BBa_J61002 J61002]
|align="center" |XbaI
|align="center" |XbaI
|align="center" |PstI
|align="center" |PstI
|align="center" |Vector
|align="center" |Vector
|align="center" |-
|align="center" |-
-
|align="center" |2073 bp
+
|align="center" |2073  
|align="center" |pSB1A2<br>[[Image:Icon_plasmid.png]]
|align="center" |pSB1A2<br>[[Image:Icon_plasmid.png]]
|align="center" |
|align="center" |
Line 2,130: Line 2,453:
|style="background: #C5D0EA;"|D192
|style="background: #C5D0EA;"|D192
|align="center" |MP144.2
|align="center" |MP144.2
-
|align="center" |[http://partsregistry.org/Part:BBa_pJ61002 pJ61002]
+
|align="center" |[http://partsregistry.org/Part:BBa_J61002 J61002]
|align="center" |EcoRI
|align="center" |EcoRI
|align="center" |XbaI
|align="center" |XbaI
Line 2,260: Line 2,583:
|style="background: #C5D0EA;"|D202
|style="background: #C5D0EA;"|D202
|align="center" |MP101.1
|align="center" |MP101.1
-
|align="center" |[http://partsregistry.org/Part:BBa_pJ61002 pJ61002]
+
|align="center" |[http://partsregistry.org/Part:BBa_J61002 J61002]
|align="center" |EcoRI
|align="center" |EcoRI
|align="center" |nothing
|align="center" |nothing
|align="center" |
|align="center" |
|align="center" |
|align="center" |
-
|align="center" |3037
+
|align="center" |2984
|align="center" |
|align="center" |
|align="center" |
|align="center" |
|align="center" |25
|align="center" |25
|align="center"|[[Team:Paris/September 8|September 8]]
|align="center"|[[Team:Paris/September 8|September 8]]
-
 
-
|}
 
-
 
-
== Strains (glycerol stock) ==
 
-
{|
 
-
|- style="background: #649CD7;"
 
-
! colspan="6" style="background: #649CD7;" | Strains glycerol stock
 
-
|- style="background: #649CD7; text-align: center;"
 
-
|width=5%| Number
 
-
|width=15%| Name
 
-
|Width=25%| Biobricks
 
-
|width=30%| Description
 
-
|width=15%| Verified clone
 
-
|width=5%| Date
 
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |S103
+
|style="background: #C5D0EA;"|D203
-
|align="center" |[http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3]
+
|align="center" |MP101.2
-
|align="center" |[[Image:Part_icon_regulatory.png]]
+
|align="center" |[http://partsregistry.org/Part:BBa_J61002 J61002]
-
|align="center" |AraC pBAD
+
|align="center" |BspHI
-
|
+
|align="center" |nothing
-
|align="center" |[[Team:Paris/July 23|July 23]]
+
|align="center" |
 +
|align="center" |
 +
|align="center" |1870<br>1008<br>105
 +
|align="center" |
 +
|align="center" |
 +
|align="center" |
 +
|align="center"|[[Team:Paris/September 11|September 11]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |S106
+
|style="background: #C5D0EA;"|D204
-
|align="center" |[http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2]
+
|align="center" |MP144
-
|align="center" |[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
|align="center" |[http://partsregistry.org/Part:BBa_J61002 J61002]
-
|align="center" |ECFP (RBS+LVA+ Double Term)
+
|align="center" |SpeI
-
|
+
|align="center" |PstI
-
|align="center" |[[Team:Paris/July 23|July 23]]
+
|align="center" |
 +
|align="center" |
 +
|align="center" |
 +
|align="center" |
 +
|align="center" |
 +
|align="center" |
 +
|align="center"|[[Team:Paris/September 22|September 22]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |S107
+
|style="background: #C5D0EA;"|D205
-
|align="center" |[http://partsregistry.org/Part:BBa_B0034 B0034] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
|align="center" |MP101
-
|align="center" |[[Image:Part_icon_rbs.png]]
+
|align="center" |[http://partsregistry.org/Part:BBa_J61002 J61002]
-
|align="center" |Strongest RBS (Efficiency = 1)
+
|align="center" |XbaI
-
|
+
|align="center" |PstI
-
|align="center" |[[Team:Paris/July 23|July 23]]
+
|align="center" |
 +
|align="center" |
 +
|align="center" |
 +
|align="center" |
 +
|align="center" |
 +
|align="center" |
 +
|align="center"|[[Team:Paris/September 22|September 22]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |S108
+
|style="background: #C5D0EA;"|D206
-
|align="center"|[http://partsregistry.org/Part:BBa_R0079 R0079] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
|align="center" |MP179
-
|align="center"|[[Image:Part_icon_regulatory.png]]
+
|align="center" |
-
|align="center"|promoter pLas (lasr & PAI regulated)
+
|align="center" |XbaI
-
|align="center"|
+
|align="center" |PstI
-
|align="center" |[[Team:Paris/July 23|July 23]]
+
|align="center" |
 +
|align="center" |
 +
|align="center" |
 +
|align="center" |
 +
|align="center" |
 +
|align="center" |
 +
|align="center"|[[Team:Paris/September 22|September 22]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |S109
+
|style="background: #C5D0EA;"|D207
-
|align="center"|[http://partsregistry.org/Part:BBa_J23101 J23101] in [http://partsregistry.org/Part:BBa_J61002 pJ61002 ]
+
|align="center" |MP182.3
-
|align="center"|[[Image:Part_icon_regulatory.png]]
+
|align="center" |
-
|align="center"|Strongest Constitutive promoter
+
|align="center" |XbaI
-
|align="center"|
+
|align="center" |PstI
-
|align="center" |[[Team:Paris/July 23|July 23]]
+
|align="center" |
 +
|align="center" |
 +
|align="center" |
 +
|align="center" |
 +
|align="center" |
 +
|align="center" |
 +
|align="center"|[[Team:Paris/September |September ]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |S110
+
|style="background: #C5D0EA;"|D208
-
|align="center"|[http://partsregistry.org/Part:BBa_J23109 J23109] in [http://partsregistry.org/Part:BBa_J61002 pJ61002 ]
+
|align="center" |MP175
-
|align="center"|[[Image:Part_icon_regulatory.png]]
+
|align="center" |
-
|align="center"|Weak Constitutive promoter
+
|align="center" |EcoRI
-
|align="center"|
+
|align="center" |SpeI
-
|align="center" |[[Team:Paris/July 23|July 23]]
+
|align="center" |
 +
|align="center" |
 +
|align="center" |
 +
|align="center" |
 +
|align="center" |
 +
|align="center" |
 +
|align="center"|[[Team:Paris/September |September ]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |S111
+
|style="background: #C5D0EA;"|D209
-
|align="center"|[http://partsregistry.org/Part:BBa_R0040 R0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
|align="center" |MP144
-
|align="center"|[[Image:Part_icon_regulatory.png]]
+
|align="center" |[http://partsregistry.org/Part:BBa_J61002 J61002]
-
|align="center"|tetR repressible promoter
+
|align="center" |EcoRI
-
|align="center"|
+
|align="center" |SpeI
-
|align="center" |[[Team:Paris/July 23|July 23]]
+
|align="center" |
 +
|align="center" |
 +
|align="center" |
 +
|align="center" |
 +
|align="center" |
 +
|align="center" |
 +
|align="center"|[[Team:Paris/September |September ]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |S112
+
|style="background: #C5D0EA;"|D210
-
|align="center"|[http://partsregistry.org/Part:BBa_S03154 S03154] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
|align="center" |MP178
-
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]
+
|align="center" |
-
|align="center"|B0034(rbs) ->LasI
+
|align="center" |EcoRI
-
|align="center"|
+
|align="center" |SpeI
-
|align="center" |[[Team:Paris/July 23|July 23]]
+
|align="center" |
-
|- style="background: #dddddd;" 
+
|align="center" |
-
| style="background: #D4E2EF;" |S113
+
|align="center" |
-
|align="center"|[http://partsregistry.org/Part:BBa_S03879 S03879] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
|align="center" |
-
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]
+
|align="center" |
-
|align="center"|B0034(rbs) -> TetR
+
|align="center" |
-
|align="center"|
+
|align="center"|[[Team:Paris/September |September ]]
-
|align="center" |[[Team:Paris/July 23|July 23]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S114
+
-
|align="center"|[http://partsregistry.org/Part:BBa_C0079 C0079] in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
+
-
|align="center"|[[Image:Icon_coding.png]]
+
-
|align="center"|lasR activator with LVA Tag
+
-
|align="center"|
+
-
|align="center" |[[Team:Paris/July 23|July 23]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S115
+
-
|align="center"|[http://partsregistry.org/Part:BBa_C0179 C0179] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
-
|align="center"|[[Image:Icon_coding.png]]
+
-
|align="center"|lasR activator
+
-
|align="center"|
+
-
|align="center" |[[Team:Paris/July 23|July 23]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S116
+
-
|align="center"|[http://partsregistry.org/Part:BBa_E0030 E0030] in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
+
-
|align="center"|[[Image:Part_icon_reporter.png]]
+
-
|align="center"|YFP
+
-
|align="center"|
+
-
|align="center" |[[Team:Paris/July 23|July 23]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S117
+
-
|align="center"|[http://partsregistry.org/Part:BBa_E0040 E0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
-
|align="center"|[[Image:Part_icon_reporter.png]]
+
-
|align="center"|GFP
+
-
|align="center"|
+
-
|align="center" |[[Team:Paris/July 23|July 23]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S118
+
-
|align="center"|[http://partsregistry.org/Part:BBa_E1010 E1010] in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
+
-
|align="center"|[[Image:Part_icon_reporter.png]]
+
-
|align="center"|mRFP
+
-
|align="center"|
+
-
|align="center" |[[Team:Paris/July 23|July 23]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S119
+
-
|align="center"|OmpR M57I
+
-
|align="center"|[[Image:Icon_coding.png]]
+
-
|align="center"|
+
-
|
+
-
|align="center" |[[Team:Paris/July 23|July 23]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S120
+
-
|align="center"|EnvZ I281S
+
-
|align="center"|[[Image:Icon_coding.png]]
+
-
|align="center"|
+
-
|
+
-
|align="center" |[[Team:Paris/July 23|July 23]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S121
+
-
|align="center"|[http://partsregistry.org/Part:BBa_C0040 C0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
-
|align="center"|[[Image:Icon_coding.png]]
+
-
|align="center"|tetR repressor
+
-
|align="center"|
+
-
|align="center" |[[Team:Paris/July 23|July 23]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S122
+
-
|align="center"|[http://partsregistry.org/Part:pSB1A3  pSB1A3]
+
-
|align="center"|[[Image:Icon_plasmid.png]]
+
-
|align="center"|
+
-
|
+
-
|align="center" |[[Team:Paris/July 23|July 23]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S123
+
-
|align="center"|L102 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
-
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]] 
+
-
|align="center"|Strong rbs - YFP<br>D129 (BV) -  D118 (BI)
+
-
|align="center"|
+
-
|align="center" |[[Team:Paris/August 1|Aug 1]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S124
+
-
|align="center"|L103 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
-
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]
+
-
|align="center"|Strong rbs - mRFP<br>D129 (BV) - D122 (BI)
+
-
|align="center"|
+
-
|align="center" |[[Team:Paris/August 1|Aug 1]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S125
+
-
|align="center"|L104 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
-
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]
+
-
|align="center"|Strong rbs - lasR activator<br>D129 (BV) - D114 (BI)
+
-
|align="center"|
+
-
|align="center" |[[Team:Paris/August 1|Aug 1]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S126
+
-
|align="center"|L105 in [http://partsregistry.org/Part:J61002 J61002]
+
-
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
-
|align="center"|Strong promoter - ECFP<br>D123 (BV) - D130 (BI)
+
-
|align="center"|
+
-
|align="center" |[[Team:Paris/August 1|Aug 1]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S127
+
-
|align="center"|L106 in [http://partsregistry.org/Part:J61002 J61002]
+
-
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
-
|align="center"|Strong promoter -  gfp Tripart<br>D123 (BV) - D131 (BI)
+
-
|align="center"|
+
-
|align="center" |[[Team:Paris/August 1|Aug 1]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S128
+
-
|align="center"|L107 in [http://partsregistry.org/Part:J61002 J61002]
+
-
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
-
|align="center"|Strongest promoter - ECFP<br>D103 (BV) - D130 (BI)
+
-
|align="center"|
+
-
|align="center" |[[Team:Paris/August 1|Aug 1]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S129
+
-
|align="center"|L108 in [http://partsregistry.org/Part:J61002 J61002]
+
-
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
-
|align="center"|Strong promoter - GFP tripart<br>D103 (BV) - D131 (BI)
+
-
|align="center"|
+
-
|align="center" |[[Team:Paris/August 1|Aug 1]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S130
+
-
|align="center"|L110 in [http://partsregistry.org/Part:J61002 J61002]
+
-
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
-
|align="center"|Medium promoter - gfp tripart<br>D124 (BV) - D131 (BI)
+
-
|align="center"|
+
-
|align="center" |[[Team:Paris/August 1|Aug 1]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S131
+
-
|align="center"|L111 in [http://partsregistry.org/Part:J61002 J61002]
+
-
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
-
|align="center"|Weak promoter - ECFP<br>D104 (BV) - D130 (BI)
+
-
|align="center"|
+
-
|align="center" |[[Team:Paris/August 1|Aug 1]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S132
+
-
|align="center"|L112 in [http://partsregistry.org/Part:J61002 J61002]
+
-
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
-
|align="center"|Weak promoter - gfp Tripart<br>D104 (BV) - D131 (BI)
+
-
|align="center"|
+
-
|align="center" |[[Team:Paris/August 1|Aug 1]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S133
+
-
|align="center"|L115 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
-
|align="center"|[[Image:Part_icon_reporter.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
-
|align="center"|pLas - ECFP <br>D105 (BV) - D130 (BI)
+
-
|align="center"|
+
-
|align="center" |[[Team:Paris/August 1|Aug 1]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S134
+
-
|align="center"|L116 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
-
|align="center"|[[Image:Part_icon_reporter.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
-
|align="center"|pLas - gfp Tripart<br>D105 (BV) - D131 (BI)
+
-
|align="center"|
+
-
|align="center" |[[Team:Paris/August 1|Aug 1]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S135
+
-
|align="center"|L117 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
+
-
|align="center"|[[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
-
|align="center"|yfp - Double Terminator<br>D125 (FV) - D117 (FI)
+
-
|align="center"|
+
-
|align="center" |[[Team:Paris/August 1|Aug 1]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S136
+
-
|align="center"|L118 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
+
-
|align="center"|[[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
-
|align="center"|rfp - Double Terminator<br>D125 (FV) - D121 (FI)
+
-
|align="center"|
+
-
|align="center" |[[Team:Paris/August 1|Aug 1]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S137
+
-
|align="center"|L119 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
+
-
|align="center"|[[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
-
|align="center"|LasR activator + LVA - Double terminator<br>D125 (FV) - D113 (FI)
+
-
|align="center"|
+
-
|align="center" |[[Team:Paris/August 1|Aug 1]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S138
+
-
|align="center"|L121 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
-
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
-
|align="center"|pTet - gfp Tripart<br>D106 (BV) - D131 (BI)
+
-
|align="center"|
+
-
|align="center" |[[Team:Paris/August 1|Aug 1]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S139
+
-
|align="center"|L124 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
-
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]
+
-
|align="center"|Strongest rbs - mRFP<br>D102 (BV) - D122 (BI)
+
-
|align="center"|
+
-
|align="center" |[[Team:Paris/August 1|Aug 1]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S140
+
-
|align="center"|L125 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
-
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]
+
-
|align="center"|Strongest rbs - yfp<br>D102 (BV) - D118 (BI)
+
-
|align="center"|
+
-
|align="center" |[[Team:Paris/August 1|Aug 1]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S141
+
-
|align="center"|MG1655
+
-
|align="center"|
+
-
|align="center"|
+
-
|align="center"|
+
-
|align="center" |[[Team:Paris/August 7|Aug 7]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S142
+
-
|align="center"|[http://partsregistry.org/Part:J61002  J61002]
+
-
|align="center"|[[Image:Icon_plasmid.png]]
+
-
|align="center"|
+
-
|
+
-
|align="center" |[[Team:Paris/August 7|Aug 7]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S143
+
-
|align="center"|L128 in [http://partsregistry.org/Part:J61002 J61002]
+
-
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
+
-
|align="center"|pFlgA-RFP<br>D132 (FI) -  D136 (FV)
+
-
|align="center"|MP144.3 ; MP144.4
+
-
|align="center"|[[Team:Paris/August 12|August 12]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S144
+
-
|align="center"|L129 in [http://partsregistry.org/Part:J61002 J61002]
+
-
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
+
-
|align="center"|pFlgB-RFP<br>D133 (FI) -  D136 (FV)
+
-
|align="center"|MP145.6 ; MP145.7
+
-
|align="center"|[[Team:Paris/August 12|August 12]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S145
+
-
|align="center"|L130 in [http://partsregistry.org/Part:J61002 J61002]
+
-
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
+
-
|align="center"|pFlhB-RFP<br>D134 (FI) -  D136 (FV)
+
-
|align="center"|MP146.1 ; MP146.2
+
-
|align="center"|[[Team:Paris/August 12|August 12]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S146
+
-
|align="center"|L100 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
-
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
-
|align="center"|rbs TetR - ECFP<br>D110 (BV) -  D130 (BI)
+
-
|
+
-
|align="center"|[[Team:Paris/August 13|August 13]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S147
+
-
|align="center"|L101 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
-
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
-
|align="center"|rbs TetR - GFP tripart<br>D110 (BV) -  D131 (BI)
+
-
|
+
-
|align="center"|[[Team:Paris/August 13|August 13]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S148
+
-
|align="center"|L114 in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
+
-
|align="center"| [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
-
|align="center"|AracpBAD - gfp tripart<br>D126 (BV) - D131 (BI)
+
-
|
+
-
|align="center"|[[Team:Paris/August 13|August 13]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S149
+
-
|align="center"|L120 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
-
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
-
|align="center"|tetR repressible promoter - ECFP<br>D106 (BV) - D130 (BI)
+
-
|
+
-
|align="center"|[[Team:Paris/August 13|August 13]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S150
+
-
|align="center"|L122 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
-
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
-
|align="center"|RBS-lasI - ECFP <br>D107 (BV) - D130 (BI)
+
-
|
+
-
|align="center"|[[Team:Paris/August 13|August 13]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S151
+
-
|align="center"|L123 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
-
|align="center"| [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
-
|align="center"|RBS lasI - gfp tripart<br>D107 (BV) - D131 (BI)
+
-
|
+
-
|align="center"|[[Team:Paris/August 13|August 13]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S152
+
-
|align="center"|L126 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
-
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]
+
-
|align="center"|Strongest RBS (1)- LasR activator (+LVA)<br>D102 (BV) - D114 (BI)
+
-
|
+
-
|align="center"|[[Team:Paris/August 13|August 13]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S153
+
-
|align="center"|L132 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
-
|align="center"|[[Image:Icon_coding.png]]
+
-
|align="center"|flhDC <br> D142(FV) - D139(FI)
+
-
|
+
-
|align="center"|[[Team:Paris/August 13|August 13]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S154
+
-
|align="center"|L133 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
-
|align="center"|[[Image:Icon_coding.png]]
+
-
|align="center"|OmpR*<br> D142(FV) - D140(FI)
+
-
|
+
-
|align="center"|[[Team:Paris/August 13|August 13]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S154
+
-
|align="center"|L133.1 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
-
|align="center"|[[Image:Icon_coding.png]]
+
-
|align="center"|OmpR*<br> D142(FV) - D140(FI)
+
-
|WT sequence
+
-
|align="center"|[[Team:Paris/August 19|August 19]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S155
+
-
|align="center"|L134 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
-
|align="center"|[[Image:Icon_coding.png]]
+
-
|align="center"| EnvZ*<br> D142(FV) - D141(FI)
+
-
|
+
-
|align="center"|[[Team:Paris/August 13|August 13]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S155
+
-
|align="center"|L134.2 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
-
|align="center"|[[Image:Icon_coding.png]]
+
-
|align="center"| EnvZ*<br> D142(FV) - D141(FI)
+
-
|lacks about 300 bp in EnvZ*
+
-
|align="center"|[[Team:Paris/August 19|August 19]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S156
+
-
|align="center"|L138 in [http://partsregistry.org/Part:pSB3K3 pSB3K3]
+
-
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
-
|align="center"|gfp generator (E0240)<br> D137(FV) - D138(FI)
+
-
|
+
-
|align="center"|[[Team:Paris/August 13|August 13]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S157
+
-
|align="center"|E0240
+
-
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
-
|align="center"|GFP generator
+
-
|align="center"|
+
-
|align="center"|[[Team:Paris/August 15|August 15]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S158
+
-
|align="center"|pSB3K3
+
-
|align="center"|[[Image:Icon_plasmid.png]]
+
-
|align="center"|plasmid
+
-
|align="center"|
+
-
|align="center"|[[Team:Paris/August 15|August 15]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S159.1
+
-
|rowspan="2" align="center"|L139.1 in in [http://partsregistry.org/Part:pSB3K3 pSB3K3]
+
-
|rowspan="2" align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
-
|rowspan="2" align="center"|E0240 RBS+<br> D140 (PstI) - D144 (ES)
+
-
|align="center"|seems to have the plasmid without the insert and one with the insert
+
-
|rowspan="2" align="center"|[[Team:Paris/August 15|August 15]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S159.2
+
-
|align="center"|incorrect clone
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S160.1
+
-
|rowspan="3" align="center"|L141.1 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
-
|rowspan="3" align="center"|[[Image:Icon_coding.png]]
+
-
|rowspan="3" align="center"|flhD<br> D142 (ES) - D145 (ES)
+
-
|align="center"|has not been sequenced
+
-
|rowspan="3" align="center"|[[Team:Paris/August 15|August 15]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S160.2
+
-
|align="center"|has not been sequenced
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S160.3
+
-
|align="center"|has not been sequenced
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S161.1
+
-
|rowspan="2" align="center"|L142.7 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
-
|rowspan="2" align="center"|[[Image:Icon_regulatory.png]]
+
-
|rowspan="2" align="center"|flhDC+promotor<br> D143 (ES) - D145 (ES)
+
-
|align="center"|does not seem to have the insert
+
-
|rowspan="2" align="center"|[[Team:Paris/August 15|August 15]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S161.2
+
-
|align="center"|incorrect clone
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |S162
+
-
|align="center"|MP162.1 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
+
-
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
-
|align="center"|rbs-lasR + double terminator<br> D147 (ES) - D125 (EX)
+
-
|align="center"|
+
-
|align="center"|[[Team:Paris/August 18|August 18]]
+
-
|- style="background: #dddddd;"
+
-
| style="background: #D4E2EF;" |S163
+
-
|align="center"|B0032 in pSB1A2
+
-
|align="center"|[[Image:Icon_rbs.png]]
+
-
|align="center"|medium rbs (efficiency = 0,3)
+
-
|align="center"|
+
-
|align="center"|[[Team:Paris/August 20|August 20]]
+
-
|- style="background: #dddddd;"
+
-
| style="background: #D4E2EF;" |S164
+
-
|align="center"|E0422
+
-
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
-
|align="center"|strongest rbs ECFP LVA+ double terminator
+
-
|align="center"|
+
-
|align="center"|[[Team:Paris/August 20|August 20]]
+
-
|- style="background: #dddddd;"
+
-
| style="background: #D4E2EF;" |S165
+
-
|align="center"|E0430
+
-
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
-
|align="center"|strongest rbs YFP LVA- double terminator
+
-
|align="center"|
+
-
|align="center"|[[Team:Paris/August 20|August 20]]
+
-
|- style="background: #dddddd;"
+
-
| style="background: #D4E2EF;" |S166
+
-
|align="center"|E0432
+
-
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
-
|align="center"|strongest rbs YFP LVA+ double terminator
+
-
|align="center"|
+
-
|align="center"|[[Team:Paris/August 20|August 20]]
+
-
|- style="background: #dddddd;"
+
-
| style="background: #D4E2EF;" |S167
+
-
|align="center"|E0420
+
-
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>
+
-
|align="center"|strongest rbs ECFP LVA- double terminator
+
-
|align="center"|
+
-
|align="center"|[[Team:Paris/August 20|August 20]]
+
-
|- style="background: #dddddd;"
+
-
| style="background: #D4E2EF;" |S168
+
-
|align="center"|I732078
+
-
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+
-
|align="center"|strongest rbs mRFP LVA+ double terminator
+
-
|align="center"|
+
-
|align="center"|[[Team:Paris/August 20|August 20]]
+
|}
|}

Latest revision as of 14:46, 29 October 2008

Contents

Last number used

Miniprep Strain Ligation Digestion PCR
216 216 234 202 154

(last checking on 19th october)

Freezer

PCR products

PCR products
Number Name Oligo F Oligo R Template Length Comments Product Left ? [DNA]µg/mL Date
PCR124 pFlgA O100 O101 - 261pb yes 0.5 August 7
PCR125 pFlgB O102 O103 - 261pb yes 0.5 August 7
PCR126 pFlhB O108 O109 - 260pb yes 0.5 August 7
PCR127 FlhDC O132 O131 MG1655 no August 8
PCR128 OmpR* O138 O139 Strain OmpR* 700pb yes 150 August 8
PCR129 EnvZ* O126 O127 Strain EnvZ* 1400pb yes 100 August 8
PCR130 E0240 rbs+ O141 O140 MP143 900pb no August 11
PCR131 FlhD rbs- O132 O133 MG1655 350pb no August 11
PCR132 pFlhD rbs- O130 O131 MG1655 600pb no August 11
PCR133 FlhDC + prom O110 O131 MG1655 1300pb no August 11
PCR134 FlhDC + prom O111 O131 MG1655 no August 11
PCR135 pFliL O124 O125 MG1655 yes August 11
PCR136 pFlhDC O110 O113 MG1655 yes August 11
PCR137 pFlhDC O 111 O 113 MG1655 yes August 11
PCR138 FlhDC (RBS -) O 132 O 131 MG 1655 yes August 15
PCR139 pFlhDC (URI) O 110 O 113 PCR 130 yes August 15
PCR140 FlhDC (RBS -) O 132 O 131 PCR 130 yes August 15
PCR141 Gene FlhDC + rbs nat O 134 O 131 MG1655 1000pb yes August 26
PCR142 pFliA + rbs nat O 145 O 144 MG1655 325pb yes August 26
PCR143 pFliA sans rbs O 145 O 146 MG1655 310pb yes August 26
PCR144 pFliA + Gene FliA O 145 O 151 MG1655 1100pb yes August 26
PCR145 FliA1 O 148 O 152 MG1655 150pb yes August 26
PCR146 FliA2 O 153 O 150 MG1655 670pb yes August 26
PCR 147 EnvZ* O 126 O 127 S 120 1412 bp yes 150 August 26
PCR 148 OmpR* O 138 O 139 S 119 789 bp yes 101 August 26
PCR 149 pFlhDC (Soutourina +RBS) O 154 O 113 MG1655 450 bp yes August 26
PCR 151 pFLiA without nat. rbs O 145 O 146 MG1655 310 bp yes 34 August 26
PCR 152 pFliA rbs nat. O 145 O 144 MG1655 325 bp yes 19 August 26
PCR 153 pFliA+Gene FliA non-mutated O 145 O 151 MG1655 1100 bp yes 39 August 26
PCR 154 Gene FliA mutated (EcoRI/PstI) O 148 O 150 MG1655 800 bp 154.1, 154.2, 154.3 yes 50 August 26

Parts

Ligations: Constructions realised
Name/N° Plasmid Strain Miniprep Description Digestion comment Date
[http://partsregistry.org/Part:BBa_I0500 I0500] [http://partsregistry.org/Part:pSB2K3 pSB2K3] S103 MP119 Part icon regulatory.png
araC/pBad 		July 24, July 26
[http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S106 MP122 Part icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
GFP generator; strong rbs 		July 26
[http://partsregistry.org/Part:BBa_B0034 B0034 ] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S107 MP100 Part icon rbs.png
Strongest RBS (Efficiency = 1) 		July 26
[http://partsregistry.org/Part:BBa_R0079 R0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S108 MP103 Part icon regulatory.png
pLas (lasr & PAI regulated) 		July 23, July 26
[http://partsregistry.org/Part:BBa_J23101 J23101] [http://partsregistry.org/Part:BBa_J61002 J61002 ] S109 MP101 Part icon regulatory.png
Strongest Constitutive promoter 		July 22, July 23, July 26
[http://partsregistry.org/Part:BBa_J23109 J23109] [http://partsregistry.org/Part:BBa_J61002 J61002] S110 MP102 Part icon regulatory.png
Weak Constitutive promoter 		July 22, July 26
[http://partsregistry.org/Part:BBa_R0040 R0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S111 MP104 Part icon regulatory.png
pTetR repressible 		July 23, July 26
[http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S112 MP105 Part icon rbs.pngIcon coding.png
B0034(rbs) ->LasI 		July 26
[http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S113 MP106 Part icon rbs.pngIcon coding.png
B0034(rbs) -> TetR 		August 24
[http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] S114 MP107 Icon coding.png
lasR activator with LVA Tag 		July 23
[http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S115 MP108 Icon coding.png
lasR activator 		July 22, July 23, July 26
[http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] S116 MP109 Part icon reporter.png
YFP 		July 26
[http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S117 MP110 Part icon reporter.png
GFP 		July 23, July 26
[http://partsregistry.org/Part:BBa_E1010 E1010 ] [http://partsregistry.org/Part:pSB2K3 pSB2K3] S118 MP111 Part icon reporter.png
mRFP 		July 23, July 26
OmpR* M57I Strain S119 Strain MG1655
Icon coding.png
OmpR* 		Really mutated? July 23
EnvZ* I281S Strain S120 Strain MG1655
Icon coding.png
EnvZ* 		July 23
[http://partsregistry.org/Part:BBa_C0040 C0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S121 MP114 Icon coding.png
tetR repressor 		July 23
[http://partsregistry.org/Part:pSB1A3 pSB1A3] [http://partsregistry.org/Part:pSB1A3 pSB1A3] S122 MP115 Icon plasmid.png
plasmid 		July 23
L102 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S123 MP124 Part icon rbs.pngPart icon reporter.png
Strong rbs - YFP 		D129 (BV) - D118 (BI) August 1
L103 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S124 MP125 Part icon rbs.pngPart icon reporter.png
Strong rbs - mRFP 		D129 (BV) - D122 (BI) August 1
L104 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S125 MP126 Part icon rbs.pngIcon coding.png
Strong rbs - lasR activator 		D129 (BV) - D114 (BI) August 1
L105 [http://partsregistry.org/Part:J61002 J61002] S126 MP127 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
Strong promoter - ECFP 		D123 (BV) - D130 (BI) August 1
L106 [http://partsregistry.org/Part:J61002 J61002] S127 MP128 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
Strong promoter - GFP Tripart 		D123 (BV) - D131 (BI) August 1
L107 [http://partsregistry.org/Part:J61002 J61002] S128 MP129 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
Strongest promoter - ECFP 		D103 (BV) - D130 (BI) August 1
L108 [http://partsregistry.org/Part:J61002 J61002] S129 MP130 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
Strongest promoter - GFP Tripart 		D103 (BV) - D131 (BI) August 1
L110 [http://partsregistry.org/Part:J61002 J61002] S130 MP131 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
Medium promoter - GFP Tripart 		D124 (BV) - D131 (BI) August 1
L111 [http://partsregistry.org/Part:J61002 J61002] S131 MP132 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
Weak promoter - ECFP 		D104 (BV) - D130 (BI) August 1
L112 [http://partsregistry.org/Part:J61002 J61002] S132 MP133 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
Weak promoter - GFP tripart 		D104 (BV) - D131 (BI) August 1
L115 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S133 MP134 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pLas - ECFP 		D105 (BV) - D130 (BI) August 1
L116 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S134 MP135 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pLas - gfp Tripart 		D105 (BV) - D131 (BI) August 1
L117 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] S135 MP136 Part icon reporter.pngPart icon terminator.pngPart icon terminator.png
YFP - Double Terminator 		D125 (FV) - D117 (FI) August 1
L118 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] S136 MP137 Part icon reporter.pngPart icon terminator.pngPart icon terminator.png
mRFP - Double Terminator 		D125 (FV) - D121 (FI) August 1
L119 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] S137 MP138 Icon coding.pngPart icon terminator.pngPart icon terminator.png
lasR activator + LVA - Double Terminator 		D125 (FV) - D113 (FI) August 1
L121 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S138 MP139 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pTet - GFP tripart 		D106 (BV) - D131 (BI)
In Top10 strain 		August 1
L124 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S139 MP140 Part icon rbs.pngPart icon reporter.png
Strongest RBS - mRFP 		D102 (BV) - D122 (BI) August 1
L125 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S140 MP141 Part icon rbs.pngPart icon reporter.png
Strongest RBS - YFP 		D102 (BV) - D118 (BI) August 1
MG1655 Strain S141 Strain Aug 7
[http://partsregistry.org/Part:BBa_J61002 J61002] [http://partsregistry.org/Part:BBa_J61002 J61002] S142 MP123 Icon plasmid.png
plasmid 		August 5
L128 [http://partsregistry.org/Part:J61002 J61002] S143 MP144 Part icon regulatory.png
pFlgA 		D132 (FI) - D136 (FV) OK sequencing August 12
L100 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S146 MP147 Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
rbs TetR - ECFP 		D110 (BV) - D130 (BI) August 13
L114 [http://partsregistry.org/Part:pSB2K3 pSB2K3] S148 MP149 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
araC/pBad - GFP tripart 		D126 (BV) - D131 (BI) August 13
L120 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S149 MP150 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pTet - ECFP 		D106 (BV) - D130 (BI) August 13
L122 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S150 MP151 Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS-lasI - ECFP 		D107 (BV) - D130 (BI) August 13
L123 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S151 MP152 Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS lasI - GFP tripart 		D107 (BV) - D131 (BI) August 13
L126 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S152 MP153 Part icon rbs.pngIcon coding.png
Strongest RBS (1)- LasR activator (+LVA) 		D102 (BV) - D114 (BI) August 13
L132 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S153 MP154 Icon coding.png
flhDC 		D142(FV) - D139(FI) August 13
L133 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S154 MP155 Icon coding.png
OmpR* 		D142(FV) - D140(FI) WT sequence August 19
L134 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S155 MP156 Icon coding.png
EnvZ* 		D142(FV) - D141(FI) lacks about 300 bp in EnvZ* August 19
L138 [http://partsregistry.org/Part:pSB3K3 pSB3K3] S156 MP157 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
GFP generator (E0240) 		D137(FV) - D138(FI) August 13
[http://partsregistry.org/Part:BBa_E0240 E0240] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S157 MP143 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
GFP generator 		August 15
[http://partsregistry.org/Part:pSB3K3 pSB3K3] [http://partsregistry.org/Part:pSB3K3 pSB3K3] S158 MP142 Icon plasmid.png
plasmid 		low to medium copy plasmid with p15A ORI August 15
L141 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S160 MP159 Icon coding.png
flhC 		D142 (ES) - D145 (ES) has not been sequenced August 15
L151 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] S162 MP162 Icon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png
rbs-lasR + dbl ter 		D147 (FI) - D125 (FV) August 19
[http://partsregistry.org/Part:BBa_B0032 B0032] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S163 MP163 Icon rbs.png
medium rbs (efficiency = 0,3) 		August 20
[http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S164 MP121
MP164 		Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS+ ECFP+ LVA+ term 		July 24, July 26
August 20
[http://partsregistry.org/Part:BBa_E0430 E0430] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S165 MP165 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS+ YFP+ LVA- term 		August 20
[http://partsregistry.org/Part:BBa_E0432 E0432] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S166 MP166 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS+ YFP LVA+ term 		BUT be careful , it doesn't work. See registry August 20
[http://partsregistry.org/Part:BBa_E0420 E0420] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S167 MP167 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS+ ECFP LVA- term 		August 20
[http://partsregistry.org/Part:BBa_I732078 I732078] [http://partsregistry.org/Part:pSB1A3 pSB1A3] S168 MP168 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS+ mRFP LVA+ term 		August 20
L153 [http://partsregistry.org/Part:BBa_J61002 J61002] S169 MP169 Icon regulatory.pngIcon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png
Strong promoter-rbs-lasR-dble ter 		D123 (BV) - D165 (BI) August 20
L154 [http://partsregistry.org/Part:BBa_J61002 J61002] S170 MP170 Icon regulatory.pngIcon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png
strongest promoter-rbs-lasR-dble ter 		D103 (BV) - D165 (BI) August 20
L121 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S171 not done Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pTet - gfp Tripart 		D106 (BV) - D131 (BI) Same as S138 but in chemically labmade competent MG1655 bacteria. For study of doubling time of population. Aug 22
L167 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S172 MP171 Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.pngPart icon regulatory.png
gfp generator(E0240) - pTet 		August 25
L166 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S173 MP172 Part icon rbs.pngIcon coding.png
RBS(B0032) - tetR 		August 25
L160 [http://partsregistry.org/Part:BBa_J61002 J61002] S174 MP173 Icon regulatory.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA - YFP Tripart (LVA-) 		D166 (FV) - D132 (FI) Fluorescence OK August 22
L161 [http://partsregistry.org/Part:BBa_J61002 J61002] S175 MP174 Icon regulatory.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA - YFP Tripart (LVA+) 		D167 (FV) - D132 (FI) August 22
pFlhB pCR4Blunt-TOPO S178 MP175 Icon regulatory.pngIcon rbs.png
pFlhB-RBS (WT) 		OK sequencing September 04
L pCR4Blunt-TOPO S179 MP176 Icon regulatory.pngIcon rbs.png
pFliA-RBS (WT) 		pFliA with conserved Pst1 site September 04
L pCR4Blunt-TOPO S181 MP178 Icon regulatory.pngIcon rbs.png
pFliL-RBS (WT) 		OK sequencing September 04
L pCR4Blunt-TOPO S180 MP177 Icon regulatory.pngIcon rbs.png
pFlhDC-RBS (WT) 		OK sequencing September 04
L pCR4Blunt-TOPO S182 MP179 Part icon rbs.pngIcon coding.png
RBS(FlhDC) - FlhDC 		FlhDC with conserved Pst1 site September 04
L pCR4Blunt-TOPO S183 MP180 Icon coding.png
FliA2 		mutated at EcoR1 and Pst1 internal site September 04
L pCR4Blunt-TOPO S184 MP181 Icon coding.png
FliA3 		FliA mutated at EcoR1 and Pst1 site September 04
L pCR4Blunt-TOPO S185 MP182 Icon coding.png
EnvZ* 		the good one but not mutated for EcoR1 site September 04
L pCR4Blunt-TOPO S186 MP183 Icon coding.png
OmpR WT 		OmpR WT not the mutated September 04
pFlgB pCR4Blunt-TOPO S187 MP184 Icon regulatory.pngIcon rbs.png
pFlgB-RBS (WT) 		OK sequencing September 04
L pCR4Blunt-TOPO S188 MP185 Icon coding.png
OmpR WT 		OmpR WT not the mutated September 04
L179 [http://partsregistry.org/Part:J61002 J61002] S200 MP200 Icon regulatory.pngIcon rbs.pngIcon coding.png
J23101 + RBS (B0032) + tet R 		September 24
L [http://partsregistry.org/Part:J61002 J61002] S201 MP201 Icon regulatory.pngIcon rbs.pngIcon coding.png
pFlgA(RBS WT)-EnvZ* 		probably wrong according to MP208 and MP209 that have been sequenced September 24
L198 [http://partsregistry.org/Part:J61002 J61002] S207 MP207 Icon regulatory.png
pFliA 		OK sequencing October 04
L203 [http://partsregistry.org/Part:pSB3K3 pSB3K3] S208 MP208 Icon regulatory.pngIcon rbs.pngIcon coding.png
pFlgA(RBS WT)-EnvZ* 		in fact lack of pFlgA October 04
L206 [http://partsregistry.org/Part:J61002 J61002] S209 MP209 Icon regulatory.pngIcon rbs.pngIcon coding.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA(RBS WT)-EnvZ*-mRFP tripart LVA+ 		in fact lack of pFlgA October 04
L209 [http://partsregistry.org/Part:pSB3K3 pSB3K3] S210 MP210 Icon regulatory.pngIcon rbs.png
pFliL(RBS WT) 		OK sequencing October 10
L [http://partsregistry.org/Part:pSB3K3 pSB3K3] S211 MP211 Icon regulatory.pngIcon rbs.png
pFlgA(RBS WT) 		OK sequencing October 10
L208 [http://partsregistry.org/Part:pSB3K3 pSB3K3] S212 MP212 Icon regulatory.pngIcon rbs.png
pFlhB(RBS WT) 		OK sequencing October 10
L197 [http://partsregistry.org/Part:BBa_J61002 J61002] S213 MP213 Icon regulatory.pngIcon rbs.png
pFlhB(RBS WT) 		OK sequencing October 10
L200 [http://partsregistry.org/Part:BBa_J61002 J61002] S214 MP214 Icon regulatory.pngIcon rbs.png
pFliL(RBS WT) 		OK sequencing October 10
[http://partsregistry.org/Part:pSB4T5 pSB4T5] [http://partsregistry.org/Part:pSB4T5 pSB4T5] S215 MP215 Icon plasmid.png
plasmid 		low copy plasmid with pSC101 ORI October 14
[http://partsregistry.org/Part:BBa_E0434 E0434] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] S216 MP216 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
YFP tripart +AAV 		Fluorescence OK October 15
JM45 pJM45 S217 MP217 Icon plasmid.png
plasmid 		inducible FlhDC in pBad18 (ORI pBR / ampicillin resistance) October 24
L226 [http://partsregistry.org/Part:J61002 J61002] S218 MP218 Icon regulatory.pngIcon rbs.pngIcon coding.png
pFlhB(RBS WT)-EnvZ* 		October 24
L227 [http://partsregistry.org/Part:J61002 J61002] S219 MP219 Icon regulatory.pngIcon rbs.pngIcon reporter.png
pFliL(RBS WT)-GFP 		October 24
L228 [http://partsregistry.org/Part:J61002 J61002] S220 MP220 Icon regulatory.pngIcon rbs.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFliL(RBS WT)-ECFP tripart (+LVA) 		October 24
L229 [http://partsregistry.org/Part:J61002 J61002] S221 MP221 Icon regulatory.pngIcon rbs.pngIcon reporter.png
pFlgA(RBS WT)-GFP 		October 24
L232 [http://partsregistry.org/Part:J61002 J61002] S222 MP222 Icon regulatory.pngIcon rbs.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA(RBS WT)-YFP tripart (+AAV) 		October 24
L234 [http://partsregistry.org/Part:J61002 J61002] S223 MP223 Icon regulatory.pngIcon rbs.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlhB(RBS WT)-mRFP tripart (+LVA) 		October 24
L225 [http://partsregistry.org/Part:J61002 J61002] S224 MP224 Icon regulatory.pngIcon rbs.pngIcon coding.png
pFliL(RBS WT)-EnvZ* 		October 26
L233 [http://partsregistry.org/Part:J61002 J61002] S225 MP225 Icon regulatory.pngIcon rbs.pngIcon reporter.png
pFlhB(RBS WT)-GFP 		October 26
U317 pU317 S226 MP226 Icon plasmid.png
plasmid 		inducible FliA in pBad18 (ORI pBR / ampicillin resistance) October 23


NEC283 Strain S227 Strain MG1655 Δ FliA Frt October 25
NEC284 Strain S228 Strain MG1655 Δ FlhD Frt October 25
NEC285 Strain S229 Strain MG1655 Δ FlgM Frt October 25
Δ EnvZ :: Cm Strain S230 Strain MG1655 Δ EnvZ :: Cm October 25
L216 [http://partsregistry.org/Part:pSB3K3 pSB3K3] MP231 S231 Icon regulatory.pngIcon coding.png
araC/pBad-EnvZ* 		inducible EnvZ* October 27
L236 [http://partsregistry.org/Part:BBa_J61002 J61002 ] MP232 S232 Icon regulatory.pngPart icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pFliL(+WT RBS)-EnvZ*-GFP generator 		October 29
L235 [http://partsregistry.org/Part:BBa_J61002 J61002 ] MP233 S233 Icon regulatory.pngPart icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlhB(+WT RBS)-EnvZ*-GFP generator 		October 29




[http://partsregistry.org/Part:BBa_J23100 J23100] [http://partsregistry.org/Part:BBa_J61002 J61002] MP1-2007 MP116 Part icon regulatory.png
Strong constitutive promoter 		July 26
[http://partsregistry.org/Part:BBa_J23107 J23107] [http://partsregistry.org/Part:BBa_J61002 J61002] MP2-2007 MP117 Part icon regulatory.png
Medium constitutive promoter 		July 24, July 26
[http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] MP5-2007 MP120 Part icon rbs.png
Strong RBS (Efficiency = 0,6) 		July 26
[http://partsregistry.org/Part:BBa_B0015 B0015] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] MP3-2007 MP118 Part icon terminator.pngPart icon terminator.png
Double terminator 		July 24, July 26


L174 [http://partsregistry.org/Part:J61002 J61002] S MP202
Icon regulatory.pngIcon coding.png
Strong constitutive promoter-GFP generator 		J23101-E0240 September 26
L157 [http://partsregistry.org/Part:pSB1K3 pSB1AK3] Icon coding.pngIcon rbs.png
tetR-rbs (B0015) 		D125 (BV) - D162 (BI) August 23
L155 [http://partsregistry.org/Part:J61002 J61002] Icon regulatory.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
strongest promoter-gfp generator 		D164(FV) - D163 (FI) August 22
L156 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon regulatory.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pTet-gfp generator 		D161 (FV) - D163 (FI) August 22

Digestion Products

(Legend: Vector, Insert) Ø

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Obs Size Matrix (bp) Size BB (bp) Description Product in use []ng/µL Date
D100.1 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 34 10 July 26
D100.2
D101 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2076 15 10 July 29
D102.1 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2077 14 10 July 26
August 15
D102.2
D103.1 [http://partsregistry.org/Part:BBa_J23101 J23101] [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV 2100 883 27 July 26
D103.2 35
D104.1 [http://partsregistry.org/Part:BBa_J23109 J23109] [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV 2100 883 20 July 26
D104.2
D105.1 [http://partsregistry.org/Part:BBa_R0079 R0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2222 14 5 July 26
D105.2
D106.1 [http://partsregistry.org/Part:BBa_R0040 R0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2119 14 8 July 26
D106.2
D107 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2750 14 August 23 5
12 		July 26
July 23
D108.1 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 707 5 July 29
D108.2
D109 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2056 708 July 26 10 July 26
D110 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2768 14 August 23 20
8
19 		July 26
August 23
August 28
D111.1 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 725 20 July 26
D111.2
D112 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2079 703 8 September 5
D113 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] EcoRI SpeI FI 4402 779 20 July 26
D114 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] XbaI PstI BI 4003 778 20 July 26
D115.1 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 4402 746 - July 29
D115.2
D116.1 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 745 20 July 29
D116.1
D117 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI SpeI FI 3166 746 5 July 29
D118 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB2K3 pSB2K3] XbaI PstI BI 3167 745 20 July 26
D119 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2056 743 10 July 26
D120 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 742 10 July 26
D121 [http://partsregistry.org/Part:BBa_E1010 E1010] [http://partsregistry.org/Part:pSB2K3 pSB2K3] EcoRI SpeI FI 4402 704 10 July 26
D122 [http://partsregistry.org/Part:BBa_E1010 E1010] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 703 10 July 26
D123.1 [http://partsregistry.org/Part:BBa_J23100 J23100] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 26 July 26
D123.2 23
D124.1 [http://partsregistry.org/Part:BBa_J23107 J23107] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 15 July 26
D124.2
D125.1 [http://partsregistry.org/Part:BBa_B0015 B0015] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI XbaI FV 3303 15 26
10 		July 26
August 23
August 28
D125.2 24
3687
D126.1 [http://partsregistry.org/Part:BBa_I0500 I0500] [http://partsregistry.org/Part:pSB2K3 pSB2K3] SpeI PstI BV 5621 14 10 July 26
D126.2
D127.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 37 10 July 26
D127.2
D128.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2079 15 10 July 29
D128.1
D129.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2080 14 10 July 29
D129.2
D130.1 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 939 10 July 26
D130.2
D130.3
D131.1 [http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 900 8 July 26
D131.2
D131.3
D132 pFlgA - EcoRI SpeI FI - 250 5 August 6
D133 pFlgB - EcoRI SpeI FI - 250 5 August 6
D134 pFlhB - EcoRI SpeI FI - 249 5 August 6
D135 pFlhDC - EcoRI SpeI FI - problem August 6
D136 - [http://partsregistry.org/Part:BBa_J61002 J61002] EcoRI SpeI FV 2925 - August 6
August 16
D137 pSB3K3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] EcoRI SpeI FV 2727 - August 6
D138 E0240 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 899 - August 6
D139 flhDC - EcoRI PstI FI - 972 August 6
D140 E040 rbs+ - PstI PstI I - August 12
D141 flhD RBS - - EcoRI SpeI FI - August 12
D142 flhC RBS - - EcoRI SpeI FI - August 12
D143 flhDC + promoter - EcoRI SpeI FI - August 12
D144 pSB3K3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] EcoRI SpeI FI - August 12
D145 pSB1A2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI - August 12
August 15
D146 MP148 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI 2057 1609 rbs TetR - GFP tripart
D110 (BV) - D131 (BI)
Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png 		August 15
D147 MP153 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 2056 799 Strongest RBS (1)- LasR activator (+LVA)
D102 (BV) - D114 (BI)
Part icon rbs.pngIcon coding.png 		August 15
D149 pfliL - EcoRI SpeI FI - August 14
August 16
D150 pfliL - XbaI PstI BI - August 14
August 16
D152 pSB3K3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] XbaI PstI BI - August 14
D153 PCR 138 - gène flhDC - EcoRI SpeI FI - August 15
D154 PCR 140 - gène flhDC - EcoRI SpeI FI - August 15
D155 PCR 139 - pflhDC (URI) - EcoRI SpeI FI - August 15
D158 MP 155.1 - OmpR* [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI - August 15
D159 MP 156.1 - EnvZ* [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI - August 15
D161 MP 104 - pTet [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV - 39 August 16
D162 MP 114 - TetR [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI - 11 August 16
D163 MP 143 - gfp generator [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI - August 23 14
11 		August 16
August 23
D164 MP 101 - pJ23101 [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV - 3 August 16
D165 MP162.2 [http://partsregistry.org/Part:BBa_pSB1AK3 pSB1AK3] XbaI PstI BI 2057 798 rbs-lasR - double terminator
D147 (FI) - D125 (FV)
Part icon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png 		27 August 18
D166 MP 165.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV - 2942 RBS (B0034)+ YFP+ LVA- term B0015
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
59 		August 26
September 3
D179 MP3.4 or MP118 [http://partsregistry.org/Part:BBa_pSB1AK3 pSB1AK3] SpeI PstI BV B0015 (double terminator B0010-B0012)
Part icon terminator.pngPart icon terminator.png 		August 20
D180 MP101.1 [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV promoter J23101
Part icon regulatory.png 		August 20
D181 MP104.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV pTet (TetR repressible promoter)
Part icon regulatory.png 		August 20
D182 MP114.1 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI TetR
Icon coding.png 		August 20
D183 MP119.3 - XbaI PstI BI pBad promoter
Part icon regulatory.png 		August 20
D184 MP143.1 [http://partsregistry.org/Part:BBa_pSB2K3 pSB2K3] EcoRI SpeI FI gfp generator
Part icon reporter.png 		August 18
D185 MP163.1 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV B0032 RBS
Part icon rbs.png 		August 18
D187 MP168.1 [http://partsregistry.org/Part:BBa_pSB1A3 pSB1A3] EcoRI XbaI FV RBS (B0034)+ mRFP LVA+ term B0015
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
43
September 3
D188 PCR 138 - XbaI PstI Insert - 767 OmpR*
Icon coding.png 		August 26
D189 MP 101.2 [http://partsregistry.org/Part:BBa_J61002 J61002] XbaI PstI Vector - 2073 pSB1A2
Icon plasmid.png 		August 26
D191 MP114.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV 2724 tetR
Icon coding.png 		22 August 29
D192 MP144.2 [http://partsregistry.org/Part:BBa_J61002 J61002] EcoRI XbaI FV 2267 FlgA
Part icon regulatory.png 		26 August 29
D193 MP171 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 961 gfp generator(E0240) - pTet
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.pngPart icon regulatory.png 		9,5 August 29
D194 MP172.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 704 RBS(B0032) - tetR
Part icon rbs.pngIcon coding.png 		13 August 29
D195 MP172.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI 703 RBS(B0032) - tetR
Part icon rbs.pngIcon coding.png 		11 August 29
D196 MP104.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV 2119 pTet
Part icon regulatory.png 		45 September 3
D197 MP157.2 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] XbaI PstI BI 898 gfp generator(E0240)
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png 		12 September 3
D198 MP164.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV 2981 RBS (B0034)+ ECFP+ LVA+ term B0015
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png 		36 September 3
D199 MP167.1 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV 2942 RBS (B0034)+ ECFP LVA- term (B0015)
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png 		61 September 3
D200 MP171.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI 960 gfp generator(E0240) - pTet
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.pngPart icon regulatory.png 		43 September 3
D201 MP114.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV 2724 tetR
Icon coding.png 		13 August 29
D202 MP101.1 [http://partsregistry.org/Part:BBa_J61002 J61002] EcoRI nothing 2984 25 September 8
D203 MP101.2 [http://partsregistry.org/Part:BBa_J61002 J61002] BspHI nothing 1870
1008
105 		September 11
D204 MP144 [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI September 22
D205 MP101 [http://partsregistry.org/Part:BBa_J61002 J61002] XbaI PstI September 22
D206 MP179 XbaI PstI September 22
D207 MP182.3 XbaI PstI September
D208 MP175 EcoRI SpeI September
D209 MP144 [http://partsregistry.org/Part:BBa_J61002 J61002] EcoRI SpeI September
D210 MP178 EcoRI SpeI September
Retrieved from "http://2008.igem.org/Team:Paris/Notebook/Freezer"