Latest revision as of 14:46, 29 October 2008

Last number used

Miniprep	Strain	Ligation	Digestion	PCR
216	216	234	202	154

(last checking on 19th october)

Freezer

PCR products

PCR products
Number	Name	Oligo F	Oligo R	Template	Length	Comments	Product Left ?	[DNA]µg/mL	Date
PCR124	pFlgA	O100	O101	-	261pb		yes	0.5	August 7
PCR125	pFlgB	O102	O103	-	261pb		yes	0.5	August 7
PCR126	pFlhB	O108	O109	-	260pb		yes	0.5	August 7
PCR127	FlhDC	O132	O131	MG1655			no		August 8
PCR128	OmpR*	O138	O139	Strain OmpR*	700pb		yes	150	August 8
PCR129	EnvZ*	O126	O127	Strain EnvZ*	1400pb		yes	100	August 8
PCR130	E0240 rbs+	O141	O140	MP143	900pb		no		August 11
PCR131	FlhD rbs-	O132	O133	MG1655	350pb		no		August 11
PCR132	pFlhD rbs-	O130	O131	MG1655	600pb		no		August 11
PCR133	FlhDC + prom	O110	O131	MG1655	1300pb		no		August 11
PCR134	FlhDC + prom	O111	O131	MG1655			no		August 11
PCR135	pFliL	O124	O125	MG1655			yes		August 11
PCR136	pFlhDC	O110	O113	MG1655			yes		August 11
PCR137	pFlhDC	O 111	O 113	MG1655			yes		August 11
PCR138	FlhDC (RBS -)	O 132	O 131	MG 1655			yes		August 15
PCR139	pFlhDC (URI)	O 110	O 113	PCR 130			yes		August 15
PCR140	FlhDC (RBS -)	O 132	O 131	PCR 130			yes		August 15
PCR141	Gene FlhDC + rbs nat	O 134	O 131	MG1655	1000pb		yes		August 26
PCR142	pFliA + rbs nat	O 145	O 144	MG1655	325pb		yes		August 26
PCR143	pFliA sans rbs	O 145	O 146	MG1655	310pb		yes		August 26
PCR144	pFliA + Gene FliA	O 145	O 151	MG1655	1100pb		yes		August 26
PCR145	FliA1	O 148	O 152	MG1655	150pb		yes		August 26
PCR146	FliA2	O 153	O 150	MG1655	670pb		yes		August 26
PCR 147	EnvZ*	O 126	O 127	S 120	1412 bp		yes	150	August 26
PCR 148	OmpR*	O 138	O 139	S 119	789 bp		yes	101	August 26
PCR 149	pFlhDC (Soutourina +RBS)	O 154	O 113	MG1655	450 bp		yes		August 26
PCR 151	pFLiA without nat. rbs	O 145	O 146	MG1655	310 bp		yes	34	August 26
PCR 152	pFliA rbs nat.	O 145	O 144	MG1655	325 bp		yes	19	August 26
PCR 153	pFliA+Gene FliA non-mutated	O 145	O 151	MG1655	1100 bp		yes	39	August 26
PCR 154	Gene FliA mutated (EcoRI/PstI)	O 148	O 150	MG1655	800 bp	154.1, 154.2, 154.3	yes	50	August 26

Parts

Ligations: Constructions realised
Name/N°	Plasmid	Strain	Miniprep	Description	Digestion	comment	Date
[http://partsregistry.org/Part:BBa_I0500 I0500]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	S103	MP119	araC/pBad			July 24, July 26
[http://partsregistry.org/Part:BBa_E0840 E0840]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S106	MP122	GFP generator; strong rbs			July 26
[http://partsregistry.org/Part:BBa_B0034 B0034 ]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S107	MP100	Strongest RBS (Efficiency = 1)			July 26
[http://partsregistry.org/Part:BBa_R0079 R0079]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S108	MP103	pLas (lasr & PAI regulated)			July 23, July 26
[http://partsregistry.org/Part:BBa_J23101 J23101]	[http://partsregistry.org/Part:BBa_J61002 J61002 ]	S109	MP101	Strongest Constitutive promoter			July 22, July 23, July 26
[http://partsregistry.org/Part:BBa_J23109 J23109]	[http://partsregistry.org/Part:BBa_J61002 J61002]	S110	MP102	Weak Constitutive promoter			July 22, July 26
[http://partsregistry.org/Part:BBa_R0040 R0040]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S111	MP104	pTetR repressible			July 23, July 26
[http://partsregistry.org/Part:BBa_S03154 S03154]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S112	MP105	B0034(rbs) ->LasI			July 26
[http://partsregistry.org/Part:BBa_S03879 S03879]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S113	MP106	B0034(rbs) -> TetR			August 24
[http://partsregistry.org/Part:BBa_C0079 C0079]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	S114	MP107	lasR activator with LVA Tag			July 23
[http://partsregistry.org/Part:BBa_C0179 C0179]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S115	MP108	lasR activator			July 22, July 23, July 26
[http://partsregistry.org/Part:BBa_E0030 E0030]	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]	S116	MP109	YFP			July 26
[http://partsregistry.org/Part:BBa_E0040 E0040]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S117	MP110	GFP			July 23, July 26
[http://partsregistry.org/Part:BBa_E1010 E1010 ]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	S118	MP111	mRFP			July 23, July 26
OmpR* M57I	Strain	S119		Strain MG1655 OmpR*		Really mutated?	July 23
EnvZ* I281S	Strain	S120		Strain MG1655 EnvZ*			July 23
[http://partsregistry.org/Part:BBa_C0040 C0040]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S121	MP114	tetR repressor			July 23
[http://partsregistry.org/Part:pSB1A3 pSB1A3]	[http://partsregistry.org/Part:pSB1A3 pSB1A3]	S122	MP115	plasmid			July 23
L102	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S123	MP124	Strong rbs - YFP	D129 (BV) - D118 (BI)		August 1
L103	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S124	MP125	Strong rbs - mRFP	D129 (BV) - D122 (BI)		August 1
L104	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S125	MP126	Strong rbs - lasR activator	D129 (BV) - D114 (BI)		August 1
L105	[http://partsregistry.org/Part:J61002 J61002]	S126	MP127	Strong promoter - ECFP	D123 (BV) - D130 (BI)		August 1
L106	[http://partsregistry.org/Part:J61002 J61002]	S127	MP128	Strong promoter - GFP Tripart	D123 (BV) - D131 (BI)		August 1
L107	[http://partsregistry.org/Part:J61002 J61002]	S128	MP129	Strongest promoter - ECFP	D103 (BV) - D130 (BI)		August 1
L108	[http://partsregistry.org/Part:J61002 J61002]	S129	MP130	Strongest promoter - GFP Tripart	D103 (BV) - D131 (BI)		August 1
L110	[http://partsregistry.org/Part:J61002 J61002]	S130	MP131	Medium promoter - GFP Tripart	D124 (BV) - D131 (BI)		August 1
L111	[http://partsregistry.org/Part:J61002 J61002]	S131	MP132	Weak promoter - ECFP	D104 (BV) - D130 (BI)		August 1
L112	[http://partsregistry.org/Part:J61002 J61002]	S132	MP133	Weak promoter - GFP tripart	D104 (BV) - D131 (BI)		August 1
L115	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S133	MP134	pLas - ECFP	D105 (BV) - D130 (BI)		August 1
L116	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S134	MP135	pLas - gfp Tripart	D105 (BV) - D131 (BI)		August 1
L117	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]	S135	MP136	YFP - Double Terminator	D125 (FV) - D117 (FI)		August 1
L118	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]	S136	MP137	mRFP - Double Terminator	D125 (FV) - D121 (FI)		August 1
L119	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]	S137	MP138	lasR activator + LVA - Double Terminator	D125 (FV) - D113 (FI)		August 1
L121	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S138	MP139	pTet - GFP tripart	D106 (BV) - D131 (BI) In Top10 strain		August 1
L124	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S139	MP140	Strongest RBS - mRFP	D102 (BV) - D122 (BI)		August 1
L125	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S140	MP141	Strongest RBS - YFP	D102 (BV) - D118 (BI)		August 1
MG1655	Strain	S141		Strain			Aug 7
[http://partsregistry.org/Part:BBa_J61002 J61002]	[http://partsregistry.org/Part:BBa_J61002 J61002]	S142	MP123	plasmid			August 5
L128	[http://partsregistry.org/Part:J61002 J61002]	S143	MP144	pFlgA	D132 (FI) - D136 (FV)	OK sequencing	August 12
L100	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S146	MP147	rbs TetR - ECFP	D110 (BV) - D130 (BI)		August 13
L114	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	S148	MP149	araC/pBad - GFP tripart	D126 (BV) - D131 (BI)		August 13
L120	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S149	MP150	pTet - ECFP	D106 (BV) - D130 (BI)		August 13
L122	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S150	MP151	RBS-lasI - ECFP	D107 (BV) - D130 (BI)		August 13
L123	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S151	MP152	RBS lasI - GFP tripart	D107 (BV) - D131 (BI)		August 13
L126	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S152	MP153	Strongest RBS (1)- LasR activator (+LVA)	D102 (BV) - D114 (BI)		August 13
L132	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S153	MP154	flhDC	D142(FV) - D139(FI)		August 13
L133	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S154	MP155	OmpR*	D142(FV) - D140(FI)	WT sequence	August 19
L134	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S155	MP156	EnvZ*	D142(FV) - D141(FI)	lacks about 300 bp in EnvZ*	August 19
L138	[http://partsregistry.org/Part:pSB3K3 pSB3K3]	S156	MP157	GFP generator (E0240)	D137(FV) - D138(FI)		August 13
[http://partsregistry.org/Part:BBa_E0240 E0240]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S157	MP143	GFP generator			August 15
[http://partsregistry.org/Part:pSB3K3 pSB3K3]	[http://partsregistry.org/Part:pSB3K3 pSB3K3]	S158	MP142	plasmid		low to medium copy plasmid with p15A ORI	August 15
L141	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S160	MP159	flhC	D142 (ES) - D145 (ES)	has not been sequenced	August 15
L151	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]	S162	MP162	rbs-lasR + dbl ter	D147 (FI) - D125 (FV)		August 19
[http://partsregistry.org/Part:BBa_B0032 B0032]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S163	MP163	medium rbs (efficiency = 0,3)			August 20
[http://partsregistry.org/Part:BBa_E0422 E0422]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S164	MP121 MP164	RBS+ ECFP+ LVA+ term			July 24, July 26 August 20
[http://partsregistry.org/Part:BBa_E0430 E0430]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S165	MP165	RBS+ YFP+ LVA- term			August 20
[http://partsregistry.org/Part:BBa_E0432 E0432]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S166	MP166	RBS+ YFP LVA+ term		BUT be careful , it doesn't work. See registry	August 20
[http://partsregistry.org/Part:BBa_E0420 E0420]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S167	MP167	RBS+ ECFP LVA- term			August 20
[http://partsregistry.org/Part:BBa_I732078 I732078]	[http://partsregistry.org/Part:pSB1A3 pSB1A3]	S168	MP168	RBS+ mRFP LVA+ term			August 20
L153	[http://partsregistry.org/Part:BBa_J61002 J61002]	S169	MP169	Strong promoter-rbs-lasR-dble ter	D123 (BV) - D165 (BI)		August 20
L154	[http://partsregistry.org/Part:BBa_J61002 J61002]	S170	MP170	strongest promoter-rbs-lasR-dble ter	D103 (BV) - D165 (BI)		August 20
L121	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S171	not done	pTet - gfp Tripart	D106 (BV) - D131 (BI)	Same as S138 but in chemically labmade competent MG1655 bacteria. For study of doubling time of population.	Aug 22
L167	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S172	MP171	gfp generator(E0240) - pTet			August 25
L166	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S173	MP172	RBS(B0032) - tetR			August 25
L160	[http://partsregistry.org/Part:BBa_J61002 J61002]	S174	MP173	pFlgA - YFP Tripart (LVA-)	D166 (FV) - D132 (FI)	Fluorescence OK	August 22
L161	[http://partsregistry.org/Part:BBa_J61002 J61002]	S175	MP174	pFlgA - YFP Tripart (LVA+)	D167 (FV) - D132 (FI)		August 22
pFlhB	pCR4Blunt-TOPO	S178	MP175	pFlhB-RBS (WT)		OK sequencing	September 04
L	pCR4Blunt-TOPO	S179	MP176	pFliA-RBS (WT)		pFliA with conserved Pst1 site	September 04
L	pCR4Blunt-TOPO	S181	MP178	pFliL-RBS (WT)		OK sequencing	September 04
L	pCR4Blunt-TOPO	S180	MP177	pFlhDC-RBS (WT)		OK sequencing	September 04
L	pCR4Blunt-TOPO	S182	MP179	RBS(FlhDC) - FlhDC		FlhDC with conserved Pst1 site	September 04
L	pCR4Blunt-TOPO	S183	MP180	FliA2		mutated at EcoR1 and Pst1 internal site	September 04
L	pCR4Blunt-TOPO	S184	MP181	FliA3		FliA mutated at EcoR1 and Pst1 site	September 04
L	pCR4Blunt-TOPO	S185	MP182	EnvZ*		the good one but not mutated for EcoR1 site	September 04
L	pCR4Blunt-TOPO	S186	MP183	OmpR WT		OmpR WT not the mutated	September 04
pFlgB	pCR4Blunt-TOPO	S187	MP184	pFlgB-RBS (WT)		OK sequencing	September 04
L	pCR4Blunt-TOPO	S188	MP185	OmpR WT		OmpR WT not the mutated	September 04
L179	[http://partsregistry.org/Part:J61002 J61002]	S200	MP200	J23101 + RBS (B0032) + tet R			September 24
L	[http://partsregistry.org/Part:J61002 J61002]	S201	MP201	pFlgA(RBS WT)-EnvZ*		probably wrong according to MP208 and MP209 that have been sequenced	September 24
L198	[http://partsregistry.org/Part:J61002 J61002]	S207	MP207	pFliA		OK sequencing	October 04
L203	[http://partsregistry.org/Part:pSB3K3 pSB3K3]	S208	MP208	pFlgA(RBS WT)-EnvZ*		in fact lack of pFlgA	October 04
L206	[http://partsregistry.org/Part:J61002 J61002]	S209	MP209	pFlgA(RBS WT)-EnvZ*-mRFP tripart LVA+		in fact lack of pFlgA	October 04
L209	[http://partsregistry.org/Part:pSB3K3 pSB3K3]	S210	MP210	pFliL(RBS WT)		OK sequencing	October 10
L	[http://partsregistry.org/Part:pSB3K3 pSB3K3]	S211	MP211	pFlgA(RBS WT)		OK sequencing	October 10
L208	[http://partsregistry.org/Part:pSB3K3 pSB3K3]	S212	MP212	pFlhB(RBS WT)		OK sequencing	October 10
L197	[http://partsregistry.org/Part:BBa_J61002 J61002]	S213	MP213	pFlhB(RBS WT)		OK sequencing	October 10
L200	[http://partsregistry.org/Part:BBa_J61002 J61002]	S214	MP214	pFliL(RBS WT)		OK sequencing	October 10
[http://partsregistry.org/Part:pSB4T5 pSB4T5]	[http://partsregistry.org/Part:pSB4T5 pSB4T5]	S215	MP215	plasmid		low copy plasmid with pSC101 ORI	October 14
[http://partsregistry.org/Part:BBa_E0434 E0434]	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]	S216	MP216	YFP tripart +AAV		Fluorescence OK	October 15
JM45	pJM45	S217	MP217	plasmid		inducible FlhDC in pBad18 (ORI pBR / ampicillin resistance)	October 24
L226	[http://partsregistry.org/Part:J61002 J61002]	S218	MP218	pFlhB(RBS WT)-EnvZ*			October 24
L227	[http://partsregistry.org/Part:J61002 J61002]	S219	MP219	pFliL(RBS WT)-GFP			October 24
L228	[http://partsregistry.org/Part:J61002 J61002]	S220	MP220	pFliL(RBS WT)-ECFP tripart (+LVA)			October 24
L229	[http://partsregistry.org/Part:J61002 J61002]	S221	MP221	pFlgA(RBS WT)-GFP			October 24
L232	[http://partsregistry.org/Part:J61002 J61002]	S222	MP222	pFlgA(RBS WT)-YFP tripart (+AAV)			October 24
L234	[http://partsregistry.org/Part:J61002 J61002]	S223	MP223	pFlhB(RBS WT)-mRFP tripart (+LVA)			October 24
L225	[http://partsregistry.org/Part:J61002 J61002]	S224	MP224	pFliL(RBS WT)-EnvZ*			October 26
L233	[http://partsregistry.org/Part:J61002 J61002]	S225	MP225	pFlhB(RBS WT)-GFP			October 26
U317	pU317	S226	MP226	plasmid		inducible FliA in pBad18 (ORI pBR / ampicillin resistance)	October 23
NEC283	Strain	S227		Strain MG1655		Δ FliA Frt	October 25
NEC284	Strain	S228		Strain MG1655		Δ FlhD Frt	October 25
NEC285	Strain	S229		Strain MG1655		Δ FlgM Frt	October 25
Δ EnvZ :: Cm	Strain	S230		Strain MG1655		Δ EnvZ :: Cm	October 25
L216	[http://partsregistry.org/Part:pSB3K3 pSB3K3]	MP231	S231	araC/pBad-EnvZ*		inducible EnvZ*	October 27
L236	[http://partsregistry.org/Part:BBa_J61002 J61002 ]	MP232	S232	pFliL(+WT RBS)-EnvZ*-GFP generator			October 29
L235	[http://partsregistry.org/Part:BBa_J61002 J61002 ]	MP233	S233	pFlhB(+WT RBS)-EnvZ*-GFP generator			October 29
[http://partsregistry.org/Part:BBa_J23100 J23100]	[http://partsregistry.org/Part:BBa_J61002 J61002]	MP1-2007	MP116	Strong constitutive promoter			July 26
[http://partsregistry.org/Part:BBa_J23107 J23107]	[http://partsregistry.org/Part:BBa_J61002 J61002]	MP2-2007	MP117	Medium constitutive promoter			July 24, July 26
[http://partsregistry.org/Part:BBa_B0030 B0030]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	MP5-2007	MP120	Strong RBS (Efficiency = 0,6)			July 26
[http://partsregistry.org/Part:BBa_B0015 B0015]	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]	MP3-2007	MP118	Double terminator			July 24, July 26
L174	[http://partsregistry.org/Part:J61002 J61002]	S	MP202	Strong constitutive promoter-GFP generator	J23101-E0240		September 26
L157	[http://partsregistry.org/Part:pSB1K3 pSB1AK3]			tetR-rbs (B0015)	D125 (BV) - D162 (BI)		August 23
L155	[http://partsregistry.org/Part:J61002 J61002]			strongest promoter-gfp generator	D164(FV) - D163 (FI)		August 22
L156	[http://partsregistry.org/Part:pSB1A2 pSB1A2]			pTet-gfp generator	D161 (FV) - D163 (FI)		August 22

Digestion Products

(Legend: Vector, Insert) Ø

Digestion Products
Number	Product Name	Matrix Name	Enzyme 1	Enzyme 2	Obs	Size Matrix (bp)	Size BB (bp)	Description	Product in use	[]ng/µL	Date
D100.1	[http://partsregistry.org/Part:BBa_B0034 B0034]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	34			10	July 26
D100.2	[http://partsregistry.org/Part:BBa_B0034 B0034]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	34			10	July 26
D101	[http://partsregistry.org/Part:BBa_B0034 B0034]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	XbaI	FV	2076	15			10	July 29
D102.1	[http://partsregistry.org/Part:BBa_B0034 B0034]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2077	14			10	July 26 August 15
D102.2	[http://partsregistry.org/Part:BBa_B0034 B0034]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2077	14			10	July 26 August 15
D103.1	[http://partsregistry.org/Part:BBa_J23101 J23101]	[http://partsregistry.org/Part:BBa_J61002 J61002]	SpeI	PstI	BV	2100	883			27	July 26
D103.2	[http://partsregistry.org/Part:BBa_J23101 J23101]	[http://partsregistry.org/Part:BBa_J61002 J61002]	SpeI	PstI	BV	2100	883			35	July 26
D104.1	[http://partsregistry.org/Part:BBa_J23109 J23109]	[http://partsregistry.org/Part:BBa_J61002 J61002]	SpeI	PstI	BV	2100	883			20	July 26
D104.2	[http://partsregistry.org/Part:BBa_J23109 J23109]	[http://partsregistry.org/Part:BBa_J61002 J61002]	SpeI	PstI	BV	2100	883			20	July 26
D105.1	[http://partsregistry.org/Part:BBa_R0079 R0079]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2222	14			5	July 26
D105.2	[http://partsregistry.org/Part:BBa_R0079 R0079]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2222	14			5	July 26
D106.1	[http://partsregistry.org/Part:BBa_R0040 R0040]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2119	14			8	July 26
D106.2	[http://partsregistry.org/Part:BBa_R0040 R0040]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2119	14			8	July 26
D107	[http://partsregistry.org/Part:BBa_S03154 S03154]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2750	14		August 23	5 12	July 26 July 23
D108.1	[http://partsregistry.org/Part:BBa_S03154 S03154]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	707			5	July 29
D108.2	[http://partsregistry.org/Part:BBa_S03154 S03154]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	707			5	July 29
D109	[http://partsregistry.org/Part:BBa_S03154 S03154]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	SpeI	FI	2056	708		July 26	10	July 26
D110	[http://partsregistry.org/Part:BBa_S03879 S03879]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2768	14		August 23	20 8 19	July 26 August 23 August 28
D111.1	[http://partsregistry.org/Part:BBa_S03879 S03879]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	725			20	July 26
D111.2	[http://partsregistry.org/Part:BBa_S03879 S03879]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	725			20	July 26
D112	[http://partsregistry.org/Part:BBa_S03879 S03879]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	SpeI	FI	2079	703			8	September 5
D113	[http://partsregistry.org/Part:BBa_C0079 C0079]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	EcoRI	SpeI	FI	4402	779			20	July 26
D114	[http://partsregistry.org/Part:BBa_C0079 C0079]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	XbaI	PstI	BI	4003	778			20	July 26
D115.1	[http://partsregistry.org/Part:BBa_C0179 C0179]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	SpeI	FI	4402	746			-	July 29
D115.2	[http://partsregistry.org/Part:BBa_C0179 C0179]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	SpeI	FI	4402	746			-	July 29
D116.1	[http://partsregistry.org/Part:BBa_C0179 C0179]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	4403	745			20	July 29
D116.1	[http://partsregistry.org/Part:BBa_C0179 C0179]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	4403	745			20	July 29
D117	[http://partsregistry.org/Part:BBa_E0030 E0030]	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]	EcoRI	SpeI	FI	3166	746			5	July 29
D118	[http://partsregistry.org/Part:BBa_E0030 E0030]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	XbaI	PstI	BI	3167	745			20	July 26
D119	[http://partsregistry.org/Part:BBa_E0040 E0040]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	SpeI	FI	2056	743			10	July 26
D120	[http://partsregistry.org/Part:BBa_E0040 E0040]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	742			10	July 26
D121	[http://partsregistry.org/Part:BBa_E1010 E1010]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	EcoRI	SpeI	FI	4402	704			10	July 26
D122	[http://partsregistry.org/Part:BBa_E1010 E1010]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	4403	703			10	July 26
D123.1	[http://partsregistry.org/Part:BBa_J23100 J23100]	[http://partsregistry.org/Part:J61002 J61002]	SpeI	PstI	BV	2100	883			26	July 26
D123.2	[http://partsregistry.org/Part:BBa_J23100 J23100]	[http://partsregistry.org/Part:J61002 J61002]	SpeI	PstI	BV	2100	883			23	July 26
D124.1	[http://partsregistry.org/Part:BBa_J23107 J23107]	[http://partsregistry.org/Part:J61002 J61002]	SpeI	PstI	BV	2100	883			15	July 26
D124.2	[http://partsregistry.org/Part:BBa_J23107 J23107]	[http://partsregistry.org/Part:J61002 J61002]	SpeI	PstI	BV	2100	883			15	July 26
D125.1	[http://partsregistry.org/Part:BBa_B0015 B0015]	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]	EcoRI	XbaI	FV	3303	15			26 10	July 26 August 23 August 28
D125.2	[http://partsregistry.org/Part:BBa_B0015 B0015]	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]	EcoRI	XbaI	FV	3303	15			24 3687	July 26 August 23 August 28
D126.1	[http://partsregistry.org/Part:BBa_I0500 I0500]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	SpeI	PstI	BV	5621	14			10	July 26
D126.2	[http://partsregistry.org/Part:BBa_I0500 I0500]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	SpeI	PstI	BV	5621	14			10	July 26
D127.1	[http://partsregistry.org/Part:BBa_B0030 B0030]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	37			10	July 26
D127.2	[http://partsregistry.org/Part:BBa_B0030 B0030]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	37			10	July 26
D128.1	[http://partsregistry.org/Part:BBa_B0030 B0030]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	XbaI	FV	2079	15			10	July 29
D128.1	[http://partsregistry.org/Part:BBa_B0030 B0030]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	XbaI	FV	2079	15			10	July 29
D129.1	[http://partsregistry.org/Part:BBa_B0030 B0030]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2080	14			10	July 29
D129.2	[http://partsregistry.org/Part:BBa_B0030 B0030]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2080	14			10	July 29
D130.1	[http://partsregistry.org/Part:BBa_E0422 E0422]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	939			10	July 26
D130.2
D130.3
D131.1	[http://partsregistry.org/Part:BBa_E0840 E0840]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	900			8	July 26
D131.2
D131.3
D132	pFlgA	-	EcoRI	SpeI	FI	-	250			5	August 6
D133	pFlgB	-	EcoRI	SpeI	FI	-	250			5	August 6
D134	pFlhB	-	EcoRI	SpeI	FI	-	249			5	August 6
D135	pFlhDC	-	EcoRI	SpeI	FI	-		problem			August 6
D136	-	[http://partsregistry.org/Part:BBa_J61002 J61002]	EcoRI	SpeI	FV	2925	-				August 6 August 16
D137	pSB3K3	[http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3]	EcoRI	SpeI	FV	2727	-				August 6
D138	E0240	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	SpeI	FI	899	-				August 6
D139	flhDC	-	EcoRI	PstI	FI	-	972				August 6
D140	E040 rbs+	-	PstI	PstI	I	-					August 12
D141	flhD RBS -	-	EcoRI	SpeI	FI	-					August 12
D142	flhC RBS -	-	EcoRI	SpeI	FI	-					August 12
D143	flhDC + promoter	-	EcoRI	SpeI	FI	-					August 12
D144	pSB3K3	[http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3]	EcoRI	SpeI	FI	-					August 12
D145	pSB1A2	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	SpeI	FI	-					August 12 August 15
D146	MP148	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	1609	rbs TetR - GFP tripart D110 (BV) - D131 (BI)			August 15
D147	MP153	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	SpeI	FI	2056	799	Strongest RBS (1)- LasR activator (+LVA) D102 (BV) - D114 (BI)			August 15
D149	pfliL	-	EcoRI	SpeI	FI	-					August 14 August 16
D150	pfliL	-	XbaI	PstI	BI	-					August 14 August 16
D152	pSB3K3	[http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3]	XbaI	PstI	BI	-					August 14
D153	PCR 138 - gène flhDC	-	EcoRI	SpeI	FI	-					August 15
D154	PCR 140 - gène flhDC	-	EcoRI	SpeI	FI	-					August 15
D155	PCR 139 - pflhDC (URI)	-	EcoRI	SpeI	FI	-					August 15
D158	MP 155.1 - OmpR*	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	XbaI	PstI	BI	-					August 15
D159	MP 156.1 - EnvZ*	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	XbaI	PstI	BI	-					August 15
D161	MP 104 - pTet	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	SpeI	PstI	BV	-				39	August 16
D162	MP 114 - TetR	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	SpeI	FI	-				11	August 16
D163	MP 143 - gfp generator	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	XbaI	PstI	BI	-			August 23	14 11	August 16 August 23
D164	MP 101 - pJ23101	[http://partsregistry.org/Part:BBa_J61002 J61002]	SpeI	PstI	BV	-				3	August 16
D165	MP162.2	[http://partsregistry.org/Part:BBa_pSB1AK3 pSB1AK3]	XbaI	PstI	BI	2057	798	rbs-lasR - double terminator D147 (FI) - D125 (FV)		27	August 18
D166	MP 165.2	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	XbaI	FV	-	2942	RBS (B0034)+ YFP+ LVA- term B0015		59	August 26 September 3
D179	MP3.4 or MP118	[http://partsregistry.org/Part:BBa_pSB1AK3 pSB1AK3]	SpeI	PstI	BV			B0015 (double terminator B0010-B0012)			August 20
D180	MP101.1	[http://partsregistry.org/Part:BBa_J61002 J61002]	SpeI	PstI	BV			promoter J23101			August 20
D181	MP104.2	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	XbaI	FV			pTet (TetR repressible promoter)			August 20
D182	MP114.1	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	XbaI	PstI	BI			TetR			August 20
D183	MP119.3	-	XbaI	PstI	BI			pBad promoter			August 20
D184	MP143.1	[http://partsregistry.org/Part:BBa_pSB2K3 pSB2K3]	EcoRI	SpeI	FI			gfp generator			August 18
D185	MP163.1	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	SpeI	PstI	BV			B0032 RBS			August 18
D187	MP168.1	[http://partsregistry.org/Part:BBa_pSB1A3 pSB1A3]	EcoRI	XbaI	FV			RBS (B0034)+ mRFP LVA+ term B0015		43	September 3
D188	PCR 138	-	XbaI	PstI	Insert	-	767	OmpR*			August 26
D189	MP 101.2	[http://partsregistry.org/Part:BBa_J61002 J61002]	XbaI	PstI	Vector	-	2073	pSB1A2			August 26
D191	MP114.2	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	SpeI	PstI	BV		2724	tetR		22	August 29
D192	MP144.2	[http://partsregistry.org/Part:BBa_J61002 J61002]	EcoRI	XbaI	FV		2267	FlgA		26	August 29
D193	MP171	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	SpeI	FI		961	gfp generator(E0240) - pTet		9,5	August 29
D194	MP172.2	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	SpeI	FI		704	RBS(B0032) - tetR		13	August 29
D195	MP172.2	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	XbaI	PstI	BI		703	RBS(B0032) - tetR		11	August 29
D196	MP104.2	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	SpeI	PstI	BV		2119	pTet		45	September 3
D197	MP157.2	[http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3]	XbaI	PstI	BI		898	gfp generator(E0240)		12	September 3
D198	MP164.2	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	XbaI	FV		2981	RBS (B0034)+ ECFP+ LVA+ term B0015		36	September 3
D199	MP167.1	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	XbaI	FV		2942	RBS (B0034)+ ECFP LVA- term (B0015)		61	September 3
D200	MP171.2	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	XbaI	PstI	BI		960	gfp generator(E0240) - pTet		43	September 3
D201	MP114.2	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	XbaI	FV		2724	tetR		13	August 29
D202	MP101.1	[http://partsregistry.org/Part:BBa_J61002 J61002]	EcoRI	nothing			2984			25	September 8
D203	MP101.2	[http://partsregistry.org/Part:BBa_J61002 J61002]	BspHI	nothing			1870 1008 105				September 11
D204	MP144	[http://partsregistry.org/Part:BBa_J61002 J61002]	SpeI	PstI							September 22
D205	MP101	[http://partsregistry.org/Part:BBa_J61002 J61002]	XbaI	PstI							September 22
D206	MP179		XbaI	PstI							September 22
D207	MP182.3		XbaI	PstI							September
D208	MP175		EcoRI	SpeI							September
D209	MP144	[http://partsregistry.org/Part:BBa_J61002 J61002]	EcoRI	SpeI							September
D210	MP178		EcoRI	SpeI							September

Team:Paris/Notebook/Freezer

From 2008.igem.org

Latest revision as of 14:46, 29 October 2008

Contents

Last number used

Freezer

PCR products

Parts

Digestion Products

@@ Line 365: / Line 365: @@
 |}
-== Biobricks ==
+== Parts ==
 {|
 |- style="background: #649CD7;"
-| colspan="8" style="background: #649CD7;" | BioBricks
+! colspan="8" style="background: #649CD7;" | Ligations: Constructions realised
 |- style="background: #649CD7; text-align: center;"
-|width=10%| Name/N°
+|width=8%| Name/N°
-|width=10%| Plasmid
+|width=8%| Plasmid
-|width=10%| Strain
+|width=7%| Strain
-|width=10%| Miniprep
+|width=7%| Miniprep
-|width=30%|Description
+|width=32%|Description
-|width=20%| comment
+|width=20%| Digestion
+|width=8%| comment
 |width=10%| Date
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |EnvZ* I281S
+| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_I0500 I0500]
-|align="center"|Strain
+|align="center"|[http://partsregistry.org/Part:pSB2K3 pSB2K3]
-|align="center"|S120
+|align="center"|S103
-|align="center"|
+|align="center"|MP119
-|align="center"|Strain MG1655<br>[[Image:Icon_coding.png]]<br>EnvZ*
+|align="center"|[[Image:Part_icon_regulatory.png]]<br>araC/pBad
 |
-|align="center"|[[Team:Paris/July 23|July 23]]
+|
+|align="center"|[[Team:Paris/July 24|July 24]], [[Team:Paris/July 26|July 26]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |OmpR* M57I
+| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_E0840 E0840]
-|align="center"|Strain
+|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|S119
+|align="center"|S106
+|align="center"|MP122
+|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>GFP generator; strong rbs
+|
 |align="center"|
-|align="center"|Strain MG1655<br>[[Image:Icon_coding.png]]<br>OmpR*
+|align="center"|[[Team:Paris/July 26|July 26]]
-|align="center"|Really mutated?
-|align="center"|[[Team:Paris/July 23|July 23]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |MG1655
-|align="center"|Strain
-|align="center"|S141
-|align="center"|
-|align="center"|Strain
-|align="center" |
-|align="center" |[[Team:Paris/August 7|Aug 7]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |NEC283
-|align="center"|Strain
-|align="center"|S226
-|align="center"|
-|align="center"|Strain MG1655
-|align="center"|Δ FliA Frt
-|align="center"|[[Team:Paris/October 25|October 25]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |NEC284
-|align="center"|Strain
-|align="center"|S227
-|align="center"|
-|align="center"|Strain MG1655
-|align="center"|Δ FlhD Frt
-|align="center"|[[Team:Paris/October 25|October 25]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |NEC285
-|align="center"|Strain
-|align="center"|S228
-|align="center"|
-|align="center"|Strain MG1655
-|align="center"|Δ FlgM Frt
-|align="center"|[[Team:Paris/October 25|October 25]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |Δ EnvZ :: Cm
+| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_B0034 B0034 ]
-|align="center"|Strain
+|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|S229
+|align="center"|S107
-|align="center"|
+|align="center"|MP100
-|align="center"|Strain MG1655
+|align="center"|[[Image:Part_icon_rbs.png]]<br>Strongest RBS (Efficiency = 1)
-|align="center"|Δ EnvZ :: Cm
-|align="center"|[[Team:Paris/October 25|October 25]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_J61002 J61002]
-|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002]
-|align="center"|S142
-|align="center"|MP123
-|align="center"|[[Image:Icon_plasmid.png]]<br>plasmid
 |
-|align="center"|[[Team:Paris/August 5| August 5 ]]
+|
+|align="center"|[[Team:Paris/July 26|July 26]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |[http://partsregistry.org/Part:pSB1A3  pSB1A3]
+| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_R0079 R0079]
-|align="center"|[http://partsregistry.org/Part:pSB1A3  pSB1A3]
+|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|S122
+|align="center"|S108
-|align="center"|MP115
+|align="center"|MP103
-|align="center"|[[Image:Icon_plasmid.png]]<br>plasmid
+|align="center"|[[Image:Part_icon_regulatory.png]]<br>pLas (lasr & PAI regulated)
 |
-|align="center"|[[Team:Paris/July 23|July 23]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |[http://partsregistry.org/Part:pSB4T5 pSB4T5]
-|align="center"|[http://partsregistry.org/Part:pSB4T5 pSB4T5]
-|align="center"|S215
-|align="center"|MP215
-|align="center"|[[Image:icon_plasmid.png]]<br>plasmid
-|align="center"|low copy plasmid with pSC101 ORI
-|align="center"|[[Team:Paris/October 14|October 14]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |[http://partsregistry.org/Part:pSB3K3 pSB3K3]
-|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
-|align="center"|S158
-|align="center"|MP142
-|align="center"|[[Image:Icon_plasmid.png]]<br>plasmid
-|align="center"|low to medium copy plasmid with p15A ORI
-|align="center"|[[Team:Paris/August 15|August 15]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |U317
-|align="center"|pU317
-|align="center"|S225
-|align="center"|MP225
-|align="center"|[[Image:Icon_plasmid.png]]<br>plasmid
-|align="center"|FliA in pBad18 (ORI pBR  / ampicillin resistance)
-|align="center"|[[Team:Paris/October 23|October 23]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |JM45
-|align="center"|pJM45
-|align="center"|S217
-|align="center"|MP217
-|align="center"|[[Image:Icon_plasmid.png]]<br>plasmid
-|align="center"|FlhDC in pBad18 (ORI pBR / ampicillin resistance)
-|align="center"|[[Team:Paris/October 24|October 24]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_J23100 J23100]
-|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002]
-|align="center"|x
-|align="center"|MP116
-|align="center"|[[Image:Part_icon_regulatory.png]]<br>Strong constitutive promoter
 |
-|align="center"|[[Team:Paris/July 26|July 26]]
+|align="center"|[[Team:Paris/July 23|July 23]], [[Team:Paris/July 26|July 26]]
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_J23101 J23101]
@@ Line 496: / Line 420: @@
 |align="center"|MP101
 |align="center"|[[Image:Part_icon_regulatory.png]]<br>Strongest Constitutive promoter
+|
 |
 |align="center"|[[Team:Paris/July 22|July 22]], [[Team:Paris/July 23|July 23]], [[Team:Paris/July 26|July 26]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_J23107 J23107]
-|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002]
-|align="center"|x
-|align="center"|MP117
-|align="center"|[[Image:Part_icon_regulatory.png]]<br>Medium constitutive promoter
-|
-|align="center"|[[Team:Paris/July 24|July 24]], [[Team:Paris/July 26|July 26]]
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_J23109 J23109]
@@ Line 512: / Line 429: @@
 |align="center"|MP102
 |align="center"|[[Image:Part_icon_regulatory.png]]<br>Weak Constitutive promoter
+|
 |
 |align="center"|[[Team:Paris/July 22|July 22]], [[Team:Paris/July 26|July 26]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_I0500 I0500]
-|align="center"|[http://partsregistry.org/Part:pSB2K3 pSB2K3]
-|align="center"|S103
-|align="center"|MP119
-|align="center"|[[Image:Part_icon_regulatory.png]]<br>AraC pBAD
-|
-|align="center"|[[Team:Paris/July 24|July 24]], [[Team:Paris/July 26|July 26]]
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_R0040 R0040]
@@ Line 527: / Line 437: @@
 |align="center"|S111
 |align="center"|MP104
-|align="center"|[[Image:Part_icon_regulatory.png]]<br>tetR repressible promoter
+|align="center"|[[Image:Part_icon_regulatory.png]]<br>pTetR repressible
 |
-|align="center"|[[Team:Paris/July 23|July 23]], [[Team:Paris/July 26|July 26]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_R0079 R0079]
-|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|S108
-|align="center"|MP103
-|align="center"|[[Image:Part_icon_regulatory.png]]<br>promoter pLas (lasr & PAI regulated)
 |
 |align="center"|[[Team:Paris/July 23|July 23]], [[Team:Paris/July 26|July 26]]
-|- style="background: #dddddd;"
-|style="background: #D4E2EF;" |L135
-|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
-|align="center"|
-|align="center"|
-|align="center"|[[Image:Part_icon_regulatory.png]]<br>pFlgA
-|align="center"|D137 (BV) - D133 (BI)<br>don't work
-|align="center"|[[Team:Paris/August 9|August 9]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L136
-|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
-|align="center"|
-|align="center"|
-|align="center"|[[Image:Part_icon_regulatory.png]]<br>pFlgB
-|align="center"|D137 (BV) - D1343 (BI)<br>don't work
-|align="center"|[[Team:Paris/August 9|August 9]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L137
-|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
-|align="center"|
-|align="center"|
-|align="center"|[[Image:Part_icon_regulatory.png]]<br>pFlhB
-|align="center"|D137 (BV) - D135 (BI)<br>don't work
-|align="center"|[[Team:Paris/August 9|August 9]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L147
-|align="center"|[http://partsregistry.org/Part:J61002 J61002]
-|align="center"| x
-|align="center"| x
-|align="center"|[[Image:Icon_regulatory.png]]<br>pFlhDC
-|align="center"|D155 - D136<br>didn't work
-|align="center"|[[Team:Paris/August 16|August 16 ]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L
-|align="center"|[http://partsregistry.org/Part:J61002 J61002]
-|align="center"|S207
-|align="center"|MP207
-|align="center"|[[Image:Icon_regulatory.png]]<br>pFliA
-|
-|align="center"|[[Team:Paris/October 04|October 04]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L143
-|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
-|align="center"| x
-|align="center"| x
-|align="center"|[[Image:Icon_regulatory.png]]<br>pFliL
-|align="center"|D149 - D137<br>didn't work
-|align="center"|[[Team:Paris/August 14|August 14 ]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L144
-|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
-|align="center"| x
-|align="center"| x
-|align="center"|[[Image:Icon_regulatory.png]]<br>pFliL
-|align="center"|D150 - D152<br>didn't work
-|align="center"|[[Team:Paris/August 14|August 14 ]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L
-|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
-|align="center"|S211
-|align="center"|MP211
-|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlgA(RBS WT)
-|
-|align="center"|[[Team:Paris/October 10|October 10]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |pFlgB
-|align="center"|pCR4Blunt-TOPO
-|align="center"|S187
-|align="center"|MP184
-|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlgB-RBS (WT)
-|
-|align="center"|[[Team:Paris/September 04|September 04]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |pFlhB
-|align="center"|pCR4Blunt-TOPO
-|align="center"|S178
-|align="center"|MP175
-|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlhB-RBS (WT)
-|
-|align="center"|[[Team:Paris/September 04|September 04]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L
-|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
-|align="center"|S212
-|align="center"|MP212
-|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlhB(RBS WT)
-|
-|align="center"|[[Team:Paris/October 10|October 10]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L
-|align="center"|pCR4Blunt-TOPO
-|align="center"|S180
-|align="center"|MP177
-|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlhDC-RBS (WT)
-|
-|align="center"|[[Team:Paris/September 04|September 04]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L
-|align="center"|pCR4Blunt-TOPO
-|align="center"|S179
-|align="center"|MP176
-|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFliA-RBS (WT)
-|align="center"|pFliA with conserved Pst1 site
-|align="center"|[[Team:Paris/September 04|September 04]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L
-|align="center"|pCR4Blunt-TOPO
-|align="center"|S181
-|align="center"|MP178
-|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFliL-RBS (WT)
-|
-|align="center"|[[Team:Paris/September 04|September 04]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L
-|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
-|align="center"|S210
-|align="center"|MP210
-|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFliL(RBS WT)
-|
-|align="center"|[[Team:Paris/October 10|October 10]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_B0030 B0030]
-|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|x
-|align="center"|MP120
-|align="center"|[[Image:Part_icon_rbs.png]]<br>Strong RBS (Efficiency = 0,6)
-|
-|align="center"|[[Team:Paris/July 26|July 26]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_B0032 B0032]
-| align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|S163
-|align="center"|MP163
-|align="center"|[[Image:Icon_rbs.png]]<br> medium rbs (efficiency = 0,3)
-|
-|align="center"|[[Team:Paris/August 20|August 20]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_B0034 B0034 ]
-|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|S107
-|align="center"|MP100
-|align="center"|[[Image:Part_icon_rbs.png]]<br>Strongest RBS (Efficiency = 1)
-|
-|align="center"|[[Team:Paris/July 26|July 26]]
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_S03154 S03154]
@@ Line 696: / Line 448: @@
 |align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>B0034(rbs) ->LasI
 |
+|
 |align="center"|[[Team:Paris/July 26|July 26]]
 |- style="background: #dddddd;"
@@ Line 704: / Line 457: @@
 |align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>B0034(rbs) -> TetR
 |
+|
 |align="center"|[[Team:Paris/August 24|August 24]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L
-|align="center"|pCR4Blunt-TOPO
-|align="center"|S182
-|align="center"|MP179
-|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>RBS(FlhDC) - FlhDC
-|align="center"|FlhDC with conserved Pst1 site
-|align="center"|[[Team:Paris/September 04|September 04]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_C0040 C0040]
-|align="center" |[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|S121
-|align="center"|MP114
-|align="center"|[[Image:Icon_coding.png]]<br>tetR repressor
-|
-|align="center"|[[Team:Paris/July 23|July 23]]
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_C0079 C0079]
@@ Line 728: / Line 466: @@
 |align="center"|[[Image:Icon_coding.png]]<br>lasR activator with LVA Tag
 |
+|
 |align="center"|[[Team:Paris/July 23|July 23]]
 |- style="background: #dddddd;"
@@ Line 736: / Line 475: @@
 |align="center"|[[Image:Icon_coding.png]]<br>lasR activator
 |
+|
 |align="center"|[[Team:Paris/July 22|July 22]], [[Team:Paris/July 23|July 23]], [[Team:Paris/July 26|July 26]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;"  |L132
-|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|S153
-|align="center"|MP154
-|align="center"|[[Image:Icon_coding.png]]<br>flhDC
-|align="center"|D142(FV) - D139(FI)
-|align="center"|[[Team:Paris/August 13|August 13]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L133
-|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|S154
-|align="center"|MP155
-|align="center"|[[Image:Icon_coding.png]]<BR>OmpR*
-|align="center"|D142(FV) - D140(FI)<br>WT sequence
-|align="center"| [[Team:Paris/August 19|August 19]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L134
-|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|S155
-|align="center"|MP156
-|align="center"|[[Image:Icon_coding.png]]<BR> EnvZ*
-|align="center"|D142(FV) - D141(FI)<br>lacks about 300 bp in EnvZ*
-|align="center"|[[Team:Paris/August 19| August 19]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L140
-|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|
-|align="center"|
-|align="center"|[[Image:Icon_coding.png]]<br>flhD
-|align="center"|D145 (V) - D141 (I)
-|align="center"|[[Team:Paris/August 12|August 12]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L141
-|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|S160
-|align="center"|MP159
-|align="center"|[[Image:Icon_coding.png]]<br>flhC
-|align="center"|D142 (ES) - D145 (ES)<br>has not been sequenced
-|align="center"|[[Team:Paris/August 15|August 15]]
-|- style="background: #dddddd;"
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L145
-|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"| x
-|align="center"| x
-|align="center"|[[Image:Icon_coding.png]]<br>FlhDC
-|align="center"|D1543 - D145<br>didn't work
-|align="center"|[[Team:Paris/August 16|August 16 ]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L146
-|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"| x
-|align="center"| x
-|align="center"|[[Image:Icon_coding.png]]<br>FlhDC
-|align="center"|D154 - D145<br>didn't work
-|align="center"|[[Team:Paris/August 16|August 16 ]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L
-|align="center"|pCR4Blunt-TOPO
-|align="center"|S183
-|align="center"|MP180
-|align="center"|[[Image:Icon_coding.png]]<br> FliA2
-|align="center"|mutated at EcoR1 and Pst1 internal site
-|align="center"|[[Team:Paris/September 04|September 04]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L
-|align="center"|pCR4Blunt-TOPO
-|align="center"|S184
-|align="center"|MP181
-|align="center"|[[Image:Icon_coding.png]]<br> FliA3
-|align="center"|FliA mutated at EcoR1 and Pst1 site
-|align="center"|[[Team:Paris/September 04|September 04]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L
-|align="center"|pCR4Blunt-TOPO
-|align="center"|S185
-|align="center"|MP182
-|align="center"|[[Image:Icon_coding.png]]<br> EnvZ*
-|align="center"|the good one but not mutated for EcoR1 site
-|align="center"|[[Team:Paris/September 04|September 04]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L
-|align="center"|pCR4Blunt-TOPO
-|align="center"|S186
-|align="center"|MP183
-|align="center"|[[Image:Icon_coding.png]]<br> OmpR WT
-|align="center"|OmpR WT not the mutated
-|align="center"|[[Team:Paris/September 04|September 04]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L
-|align="center"|pCR4Blunt-TOPO
-|align="center"|S188
-|align="center"|MP185
-|align="center"|[[Image:Icon_coding.png]]<br> OmpR WT
-|align="center"|OmpR WT not the mutated
-|align="center"|[[Team:Paris/September 04|September 04]]
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_E0030 E0030]
@@ Line 843: / Line 484: @@
 |align="center"|[[Image:Part_icon_reporter.png]]<br>YFP
 |
+|align="center"|
 |align="center"|[[Team:Paris/July 26|July 26]]
 |- style="background: #dddddd;"
@@ Line 851: / Line 493: @@
 |align="center"|[[Image:Part_icon_reporter.png]]<br>GFP
 |
+|align="center"|
 |align="center"|[[Team:Paris/July 23|July 23]], [[Team:Paris/July 26|July 26]]
 |- style="background: #dddddd;"
@@ Line 859: / Line 502: @@
 |align="center"|[[Image:Part_icon_reporter.png]]<br>mRFP
 |
+|align="center"|
 |align="center"|[[Team:Paris/July 23|July 23]], [[Team:Paris/July 26|July 26]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_E0240 E0240]
+| style="background: #D4E2EF;" |OmpR* M57I
-|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+|align="center"|Strain
-|align="center"|S157
+|align="center"|S119
-|align="center"|MP143
-|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>GFP generator
 |align="center"|
-|align="center"|[[Team:Paris/August 15|August 15]]
+|align="center"|Strain MG1655<br>[[Image:Icon_coding.png]]<br>OmpR*
+|
+|align="center"|Really mutated?
+|align="center"|[[Team:Paris/July 23|July 23]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_E0420 E0420]
+| style="background: #D4E2EF;" |EnvZ* I281S
-|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+|align="center"|Strain
-|align="center"|S167
+|align="center"|S120
-|align="center"|MP167
+|align="center"|
-|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS+ ECFP LVA- term
+|align="center"|Strain MG1655<br>[[Image:Icon_coding.png]]<br>EnvZ*
+|
+|
+|align="center"|[[Team:Paris/July 23|July 23]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_C0040 C0040]
+|align="center" |[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+|align="center"|S121
+|align="center"|MP114
+|align="center"|[[Image:Icon_coding.png]]<br>tetR repressor
+|
 |
-|align="center"|[[Team:Paris/August 20|August 20]]
+|align="center"|[[Team:Paris/July 23|July 23]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_E0422 E0422]
+| style="background: #D4E2EF;" |[http://partsregistry.org/Part:pSB1A3  pSB1A3]
-|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+|align="center"|[http://partsregistry.org/Part:pSB1A3  pSB1A3]
-|align="center"|S164
+|align="center"|S122
-|align="center"|MP121<br>MP164
+|align="center"|MP115
-|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS+ ECFP+ LVA+ term
+|align="center"|[[Image:Icon_plasmid.png]]<br>plasmid
 |
-|align="center"|[[Team:Paris/July 24|July 24]], [[Team:Paris/July 26|July 26]]<br>[[Team:Paris/August 20|August 20]]
+|
+|align="center"|[[Team:Paris/July 23|July 23]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_E0430 E0430]
+|style="background: #D4E2EF;" |L102
 |align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|S165
+|align="center"|S123
-|align="center"|MP165
+|align="center"|MP124
-|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS+ YFP+ LVA- term
+|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]<br>Strong rbs - YFP
-|
+|align="center"|D129 (BV) -  D118 (BI)
-|align="center"|[[Team:Paris/August 20|August 20]]
+|
+|align="center"|[[Team:Paris/August 1|August 1]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_E0432 E0432]
+| style="background: #D4E2EF;" |L103
 |align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|S166
+|align="center"|S124
-|align="center"|MP166
+|align="center"|MP125
-|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS+ YFP LVA+ term
+|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]<br>Strong rbs - mRFP
-|align="center"|BUT be careful , it doesn't work. See registry
+|align="center"|D129 (BV) -  D122 (BI)
-|align="center"|[[Team:Paris/August 20|August 20]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_E0434 E0434]
-|align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
-|align="center"|S216
-|align="center"|MP216
-|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>YFP tripart +AAV
 |
-|align="center"|[[Team:Paris/October 15|October 15]]
+|align="center"|[[Team:Paris/August 1|August 1]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_E0840 E0840]
+| style="background: #D4E2EF;" |L104
 |align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|S106
+|align="center"|S125
-|align="center"|MP122
+|align="center"|MP126
-|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>gfp tri-part; strong rbs
+|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>Strong rbs - lasR activator
+|align="center"|D129 (BV) -  D114 (BI)
 |
-|align="center"|[[Team:Paris/July 26|July 26]]
+|align="center"|[[Team:Paris/August 1|August 1]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_I732078 I732078]
+| style="background: #D4E2EF;" |L105
-|align="center"|[http://partsregistry.org/Part:pSB1A3 pSB1A3]
+|align="center"|[http://partsregistry.org/Part:J61002 J61002]
-|align="center"|S168
+|align="center"|S126
-|align="center"|MP168
+|align="center"|MP127
-|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS+ mRFP LVA+ term
+|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Strong promoter - ECFP
-|
+|align="center"|D123 (BV) - D130 (BI)
-|align="center"|[[Team:Paris/August 20|August 20]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_B0015 B0015]
-|align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
-|align="center"|x
-|align="center"|MP118
-|align="center"|[[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Double terminator
 |
-|align="center"|[[Team:Paris/July 24|July 24]], [[Team:Paris/July 26|July 26]]
+|align="center"|[[Team:Paris/August 1|August 1]]
-|}
-== Devices==
-{|
-|- style="background: #649CD7;"
-! colspan="8" style="background: #649CD7;" | Ligations: Constructions realised
-|- style="background: #649CD7; text-align: center;"
-|width=8%| Name/N°
-|width=8%| Plasmid
-|width=7%| Strain
-|width=7%| Miniprep
-|width=32%|Description
-|width=20%| Digestion
-|width=8%| comment
-|width=10%| Date
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L174
+| style="background: #D4E2EF;" |L106
 |align="center"|[http://partsregistry.org/Part:J61002 J61002]
-|align="center"|S
+|align="center"|S127
-|align="center"|MP202
+|align="center"|MP128
-|align="center"|<br>[[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>Strong constitutive promoter-GFP generator
+|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Strong promoter - GFP Tripart
-|align="center"|J23101-E0240
+|align="center"|D123 (BV) - D131 (BI)
 |
-|align="center"|[[Team:Paris/September 26|September 26]]
+|align="center"|[[Team:Paris/August 1|August 1]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L142
+| style="background: #D4E2EF;" |L107
-|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|S161
-|align="center"|MP160
-|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>flhDC+promotor
-|align="center"| D143 (ES) - D145 (ES)
-|does not have <br> the insert
-|align="center"|[[Team:Paris/August 15|August 15]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L179
 |align="center"|[http://partsregistry.org/Part:J61002 J61002]
-|align="center"|S200
+|align="center"|S128
-|align="center"|MP200
+|align="center"|MP129
-|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>J23101 + RBS (B0032) + tet R
+|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Strongest promoter - ECFP
+|align="center"|D103 (BV) - D130 (BI)
 |
-|
+|align="center"|[[Team:Paris/August 1|August 1]]
-|align="center"|[[Team:Paris/September 24|September 24]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L
+| style="background: #D4E2EF;" |L108
 |align="center"|[http://partsregistry.org/Part:J61002 J61002]
-|align="center"|S201
+|align="center"|S129
-|align="center"|MP201
+|align="center"|MP130
-|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlgA(RBS WT)-EnvZ*
+|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Strongest promoter -  GFP Tripart
+|align="center"|D103 (BV) - D131 (BI)
 |
-|align="center"|probably wrong according to MP208 and MP209 that have been sequenced
+|align="center"|[[Team:Paris/August 1|August 1]]
-|align="center"|[[Team:Paris/September 24|September 24]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L
+| style="background: #D4E2EF;" |L110
-|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
+|align="center"|[http://partsregistry.org/Part:J61002 J61002]
-|align="center"|S208
+|align="center"|S130
-|align="center"|MP208
+|align="center"|MP131
-|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br> pFlgA(RBS WT)-EnvZ*
+|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Medium promoter -  GFP Tripart
+|align="center"|D124 (BV) - D131 (BI)
 |
-|align="center"|in fact lack of pFlgA
+|align="center"|[[Team:Paris/August 1|August 1]]
-|align="center"|[[Team:Paris/October 04|October 04]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L128
+| style="background: #D4E2EF;" |L111
 |align="center"|[http://partsregistry.org/Part:J61002 J61002]
-|align="center"|S143
+|align="center"|S131
-|align="center"|MP144
+|align="center"|MP132
-|align="center"|[[Image:Part_icon_regulatory.png]]<br>pFlgA
+|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Weak promoter - ECFP
-|align="center"|D132 (FI) -  D136 (FV)
+|align="center"|D104 (BV) - D130 (BI)
-|align="center"|OK sequencing
+|
-|align="center"|[[Team:Paris/August 12|August 12]]
+|align="center"|[[Team:Paris/August 1|August 1]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L129
+| style="background: #D4E2EF;" |L112
 |align="center"|[http://partsregistry.org/Part:J61002 J61002]
-|align="center"|S144
+|align="center"|S132
-|align="center"|MP145
+|align="center"|MP133
-|align="center"|[[Image:Part_icon_regulatory.png]]<br>pFlgB
+|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Weak promoter - GFP tripart
-|align="center"|D133 (FI) -  D136 (FV)
+|align="center"|D104 (BV) - D131 (BI)
-|align="center"|wrong sequencing
+|
-|align="center"|[[Team:Paris/August 12|August 12]]
+|align="center"|[[Team:Paris/August 1|August 1]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L130
+| style="background: #D4E2EF;" |L115
-|align="center"|[http://partsregistry.org/Part:J61002 J61002]
+|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|S145
+|align="center"|S133
-|align="center"|MP146
+|align="center"|MP134
-|align="center"|[[Image:Part_icon_regulatory.png]]<br>pFlhB
+|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - ECFP
-|align="center"|D134 (FI) -  D136 (FV)
+|align="center"|D105 (BV) - D130 (BI)
-|align="center"|wrong sequencing
+|
-|align="center"|[[Team:Paris/August 12|August 12]]
+|align="center"|[[Team:Paris/August 1|August 1]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L131
+| style="background: #D4E2EF;" |L116
-|align="center"|[http://partsregistry.org/Part:J61002 J61002]
+|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|
+|align="center"|S134
-|align="center"|
+|align="center"|MP135
-|align="center"|[[Image:Part_icon_regulatory.png]]<br>pFlhDC
+|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - gfp Tripart
-|align="center"|D136 (FV)- D135 (FI)
+|align="center"|D105 (BV) - D131 (BI)
-|align="center"|wrong sequencing
+|
-|align="center"|[[Team:Paris/August 8|August 8]]
+|align="center"|[[Team:Paris/August 1|August 1]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L
+| style="background: #D4E2EF;" |L117
-|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002]
+|align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
-|align="center"|S213
+|align="center"|S135
-|align="center"|MP213
+|align="center"|MP136
-|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlhB(RBS WT)
+|align="center"|[[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>YFP - Double Terminator
+|align="center"|D125 (FV) - D117 (FI)
 |
-|
+|align="center"|[[Team:Paris/August 1|August 1]]
-|align="center"|[[Team:Paris/October 10|October 10]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L
+| style="background: #D4E2EF;" |L118
-|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002]
+|align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
-|align="center"|S214
+|align="center"|S136
-|align="center"|MP214
+|align="center"|MP137
-|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFliL(RBS WT)
+|align="center"|[[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>mRFP - Double Terminator
-|
+|align="center"|D125 (FV) - D121 (FI)
 |
-|align="center"|[[Team:Paris/October 10|October 10]]
+|align="center"|[[Team:Paris/August 1|August 1]]
 |- style="background: #dddddd;"
-|style="background: #D4E2EF;" |L102
+| style="background: #D4E2EF;" |L119
-|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+|align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
-|align="center"|S123
+|align="center"|S137
-|align="center"|MP124
+|align="center"|MP138
-|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]<br>Strong rbs - YFP
+|align="center"|[[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>lasR activator + LVA - Double Terminator
-|align="center"|D129 (BV) -  D118 (BI)
+|align="center"|D125 (FV) - D113 (FI)
 |
 |align="center"|[[Team:Paris/August 1|August 1]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L103
+| style="background: #D4E2EF;" |L121
 |align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|S124
+|align="center"|S138
-|align="center"|MP125
+|align="center"|MP139
-|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]<br>Strong rbs - mRFP
+|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pTet - GFP  tripart
-|align="center"|D129 (BV) -  D122 (BI)
+|align="center"|D106 (BV) - D131 (BI)<br>In Top10 strain
 |
 |align="center"|[[Team:Paris/August 1|August 1]]
@@ Line 1,073: / Line 693: @@
 |align="center"|S139
 |align="center"|MP140
-|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]<br>Strongest RBS - rfp
+|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]<br>Strongest RBS - mRFP
 |align="center"|D102 (BV) - D122 (BI)
 |
@@ Line 1,082: / Line 702: @@
 |align="center"|S140
 |align="center"|MP141
-|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]<br>Strongest RBS - yfp
+|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]<br>Strongest RBS - YFP
 |align="center"|D102 (BV) - D118 (BI)
 |
 |align="center"|[[Team:Paris/August 1|August 1]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L104
+| style="background: #D4E2EF;" |MG1655
+|align="center"|Strain
+|align="center"|S141
+|align="center"|
+|align="center"|Strain
+|align="center" |
+|
+|align="center" |[[Team:Paris/August 7|Aug 7]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_J61002 J61002]
+|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002]
+|align="center"|S142
+|align="center"|MP123
+|align="center"|[[Image:Icon_plasmid.png]]<br>plasmid
+|
+|
+|align="center"|[[Team:Paris/August 5| August 5 ]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |L128
+|align="center"|[http://partsregistry.org/Part:J61002 J61002]
+|align="center"|S143
+|align="center"|MP144
+|align="center"|[[Image:Part_icon_regulatory.png]]<br>pFlgA
+|align="center"|D132 (FI) -  D136 (FV)
+|align="center"|OK sequencing
+|align="center"|[[Team:Paris/August 12|August 12]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |L100
 |align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|S125
+|align="center"|S146
-|align="center"|MP126
+|align="center"|MP147
-|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>Strong rbs - lasR activator
+|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>rbs TetR - ECFP
-|align="center"|D129 (BV) -  D114 (BI)
+|align="center"|D110 (BV) -  D130 (BI)
 |
-|align="center"|[[Team:Paris/August 1|August 1]]
+|align="center"|[[Team:Paris/August 13|August 13]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L148
+| style="background: #D4E2EF;" |L114
+|align="center" |[http://partsregistry.org/Part:pSB2K3 pSB2K3]
+|align="center"|S148
+|align="center"|MP149
+|align="center"| [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>araC/pBad - GFP tripart
+|align="center"|D126 (BV) - D131 (BI)
+|
+|align="center"|[[Team:Paris/August 13|August 13]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |L120
 |align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"| x
+|align="center"|S149
-|align="center"| x
+|align="center"|MP150
-|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>rbs (B0034)- OmpR*
+|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pTet - ECFP
-|align="center"|D158 - D102
+|align="center"|D106 (BV) - D130 (BI)
-|align="center"|didn't work
+|
-|align="center"|[[Team:Paris/August 16|August 16 ]]
+|align="center"|[[Team:Paris/August 13|August 13]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L149
+| style="background: #D4E2EF;" |L122
 |align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"| x
+|align="center"|S150
-|align="center"| x
+|align="center"|MP151
-|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>rbs (B0034)- EnvZ
+|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS-lasI - ECFP
-|align="center"|D159 - D102
+|align="center"|D107 (BV) - D130 (BI)
-|align="center"|didn't work
+|
-|align="center"|[[Team:Paris/August 16|August 16 ]]
+|align="center"|[[Team:Paris/August 13|August 13]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L166
+| style="background: #D4E2EF;" |L123
 |align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|S173
+|align="center"|S151
-|align="center"|MP172
+|align="center"|MP152
-|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>RBS(B0032) - tetR
+|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS lasI - GFP tripart
+|align="center"|D107 (BV) - D131 (BI)
 |
-|
+|align="center"|[[Team:Paris/August 13|August 13]]
-|align="center"|[[Team:Paris/August 25|August 25]]
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |L126
@@ Line 1,133: / Line 790: @@
 |align="center"|[[Team:Paris/August 13|August 13]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L157
+| style="background: #D4E2EF;"  |L132
-|align="center"|
+|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|
+|align="center"|S153
-|align="center"|
+|align="center"|MP154
-|align="center"|[[Image:Icon_coding.png]][[Image:Icon_rbs.png]]<br> tetR-rbs (B0015)
+|align="center"|[[Image:Icon_coding.png]]<br>flhDC
-|align="center"|D125 (BV) - D162 (BI)
+|align="center"|D142(FV) - D139(FI)
 |
-|align="center"|[[Team:Paris/August 23|August 23]]
+|align="center"|[[Team:Paris/August 13|August 13]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L117
+| style="background: #D4E2EF;" |L133
-|align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
+|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|S135
+|align="center"|S154
-|align="center"|MP136
+|align="center"|MP155
-|align="center"|[[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>yfp - Double Terminator
+|align="center"|[[Image:Icon_coding.png]]<BR>OmpR*
-|align="center"|D125 (FV) - D117 (FI)
+|align="center"|D142(FV) - D140(FI)
-|
+|align="center"|WT sequence
-|align="center"|[[Team:Paris/August 1|August 1]]
+|align="center"| [[Team:Paris/August 19|August 19]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L118
+| style="background: #D4E2EF;" |L134
-|align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
+|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|S136
+|align="center"|S155
-|align="center"|MP137
+|align="center"|MP156
-|align="center"|[[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>rfp - Double Terminator
+|align="center"|[[Image:Icon_coding.png]]<BR> EnvZ*
-|align="center"|D125 (FV) - D121 (FI)
+|align="center"|D142(FV) - D141(FI)
+|align="center"|lacks about 300 bp in EnvZ*
+|align="center"|[[Team:Paris/August 19| August 19]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |L138
+|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
+|align="center"|S156
+|align="center"|MP157
+|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>GFP generator (E0240)
+|align="center"| D137(FV) - D138(FI)
 |
-|align="center"|[[Team:Paris/August 1|August 1]]
+|align="center"|[[Team:Paris/August 13|August 13]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L119
+| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_E0240 E0240]
-|align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
+|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|S137
+|align="center"|S157
-|align="center"|MP138
+|align="center"|MP143
-|align="center"|[[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>lasR activator + LVA - Double Terminator
+|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>GFP generator
-|align="center"|D125 (FV) - D113 (FI)
+|align="center"|
+|align="center"|
+|align="center"|[[Team:Paris/August 15|August 15]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |[http://partsregistry.org/Part:pSB3K3 pSB3K3]
+|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
+|align="center"|S158
+|align="center"|MP142
+|align="center"|[[Image:Icon_plasmid.png]]<br>plasmid
 |
-|align="center"|[[Team:Paris/August 1|August 1]]
+|align="center"|low to medium copy plasmid with p15A ORI
+|align="center"|[[Team:Paris/August 15|August 15]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |L141
+|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+|align="center"|S160
+|align="center"|MP159
+|align="center"|[[Image:Icon_coding.png]]<br>flhC
+|align="center"|D142 (ES) - D145 (ES)
+|align="center"|has not been sequenced
+|align="center"|[[Team:Paris/August 15|August 15]]
 |- style="background: #dddddd;"
@@ Line 1,180: / Line 864: @@
 |align="center"|[[Team:Paris/August 19|August 19]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L159
+| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_B0032 B0032]
-|align="center"|
+| align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"| x
+|align="center"|S163
-|align="center"| x
+|align="center"|MP163
-|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> rbs-lasI-dbl ter
+|align="center"|[[Image:Icon_rbs.png]]<br> medium rbs (efficiency = 0,3)
-|align="center"|D125 (FV) - D109 (FI)
-|align="center"|didn't work
-|align="center"|[[Team:Paris/August |August ]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L138
-|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
-|align="center"|S156
-|align="center"|MP157
-|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>gfp generator (E0240)
-|align="center"| D137(FV) - D138(FI)
 |
-|align="center"|[[Team:Paris/August 13|August 13]]
+|
+|align="center"|[[Team:Paris/August 20|August 20]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L139
+| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_E0422 E0422]
-|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
+|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|S159
+|align="center"|S164
-|align="center"|MP158
+|align="center"|MP121<br>MP164
-|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>E0240 RBS+
+|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS+ ECFP+ LVA+ term
-|align="center"|D140 (PstI) - D144 (ES)
 |
-|align="center"|[[Team:Paris/August 15|August 15]]
+|align="center"|
+|align="center"|[[Team:Paris/July 24|July 24]], [[Team:Paris/July 26|July 26]]<br>[[Team:Paris/August 20|August 20]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L167
+| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_E0430 E0430]
 |align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|S172
+|align="center"|S165
-|align="center"|MP171
+|align="center"|MP165
-|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_regulatory.png]]<br>gfp generator(E0240) - pTet
+|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS+ YFP+ LVA- term
-|
 |
-|align="center"|[[Team:Paris/August 25|August 25]]
+|align="center"|
+|align="center"|[[Team:Paris/August 20|August 20]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_E0432 E0432]
+|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+|align="center"|S166
+|align="center"|MP166
+|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS+ YFP LVA+ term
+|align="center"|
+|align="center"|BUT be careful , it doesn't work. See registry
+|align="center"|[[Team:Paris/August 20|August 20]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_E0420 E0420]
+|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+|align="center"|S167
+|align="center"|MP167
+|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS+ ECFP LVA- term
+|
+|align="center"|
+|align="center"|[[Team:Paris/August 20|August 20]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_I732078 I732078]
+|align="center"|[http://partsregistry.org/Part:pSB1A3 pSB1A3]
+|align="center"|S168
+|align="center"|MP168
+|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS+ mRFP LVA+ term
+|
+|align="center"|
+|align="center"|[[Team:Paris/August 20|August 20]]
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |L153
@@ Line 1,235: / Line 937: @@
 |align="center"|[[Team:Paris/August 20|August 20]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L111
+| style="background: #D4E2EF;" |L121
-|align="center"|[http://partsregistry.org/Part:J61002 J61002]
+|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|S131
+|align="center"|S171
-|align="center"|MP132
+|align="center"|not done
-|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Weak promoter - ECFP
+|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pTet - gfp Tripart
-|align="center"|D104 (BV) - D130 (BI)
+|align="center"|D106 (BV) - D131 (BI)
-|
+|align="center"|Same as S138 but in chemically labmade competent MG1655 bacteria. For study of doubling time of  population.
-|align="center"|[[Team:Paris/August 1|August 1]]
+|align="center" |[[Team:Paris/August 22|Aug 22]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L105
+| style="background: #D4E2EF;" |L167
-|align="center"|[http://partsregistry.org/Part:J61002 J61002]
+|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|S126
+|align="center"|S172
-|align="center"|MP127
+|align="center"|MP171
-|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Strong promoter - ECFP
+|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_regulatory.png]]<br>gfp generator(E0240) - pTet
-|align="center"|D123 (BV) - D130 (BI)
 |
-|align="center"|[[Team:Paris/August 1|August 1]]
+|
+|align="center"|[[Team:Paris/August 25|August 25]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L107
+| style="background: #D4E2EF;" |L166
-|align="center"|[http://partsregistry.org/Part:J61002 J61002]
+|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|S128
+|align="center"|S173
-|align="center"|MP129
+|align="center"|MP172
-|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Strongest promoter - ECFP
+|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>RBS(B0032) - tetR
-|align="center"|D103 (BV) - D130 (BI)
 |
-|align="center"|[[Team:Paris/August 1|August 1]]
+|
-|- style="background: #dddddd;"
+|align="center"|[[Team:Paris/August 25|August 25]]
-| style="background: #D4E2EF;" |L112
-|align="center"|[http://partsregistry.org/Part:J61002 J61002]
-|align="center"|S132
-|align="center"|MP133
-|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Weak promoter - GFP tripart
-|align="center"|D104 (BV) - D131 (BI)
-|
-|align="center"|[[Team:Paris/August 1|August 1]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L110
-|align="center"|[http://partsregistry.org/Part:J61002 J61002]
-|align="center"|S130
-|align="center"|MP131
-|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Medium promoter -  gfp Tripart
-|align="center"|D124 (BV) - D131 (BI)
-|
-|align="center"|[[Team:Paris/August 1|August 1]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L106
-|align="center"|[http://partsregistry.org/Part:J61002 J61002]
-|align="center"|S127
-|align="center"|MP128
-|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Strong promoter - gfp Tripart
-|align="center"|D123 (BV) - D131 (BI)
-|
-|align="center"|[[Team:Paris/August 1|August 1]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L108
-|align="center"|[http://partsregistry.org/Part:J61002 J61002]
-|align="center"|S129
-|align="center"|MP130
-|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Strongest promoter -  gfp Tripart
-|align="center"|D103 (BV) - D131 (BI)
-|
-|align="center"|[[Team:Paris/August 1|August 1]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L155
-|align="center"|
-|align="center"|
-|align="center"|
-|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> strongest promoter-gfp generator
-|align="center"|D164(FV) - D163 (FI)
-|
-|align="center"|[[Team:Paris/August 22|August 22]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L114
-|align="center" |[http://partsregistry.org/Part:pSB2K3 pSB2K3]
-|align="center"|S148
-|align="center"|MP149
-|align="center"| [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>AracpBAD - gfp tripart
-|align="center"|D126 (BV) - D131 (BI)
-|
-|align="center"|[[Team:Paris/August 13|August 13]]
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |L160
@@ Line 1,336: / Line 982: @@
 |align="center"|[[Team:Paris/August 22|August 22]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L115
+| style="background: #D4E2EF;" |pFlhB
-|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+|align="center"|pCR4Blunt-TOPO
-|align="center"|S133
+|align="center"|S178
-|align="center"|MP134
+|align="center"|MP175
-|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - ECFP
+|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlhB-RBS (WT)
-|align="center"|D105 (BV) - D130 (BI)
+|
-|
+|align="center"|OK sequencing
-|align="center"|[[Team:Paris/August 1|August 1]]
+|align="center"|[[Team:Paris/September 04|September 04]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L120
+| style="background: #D4E2EF;" |L
-|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+|align="center"|pCR4Blunt-TOPO
-|align="center"|S149
+|align="center"|S179
-|align="center"|MP150
+|align="center"|MP176
-|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pTet - ECFP
+|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFliA-RBS (WT)
-|align="center"|D106 (BV) - D130 (BI)
+|align="center"|
-|
+|align="center"|pFliA with conserved Pst1 site
-|align="center"|[[Team:Paris/August 13|August 13]]
+|align="center"|[[Team:Paris/September 04|September 04]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L116
-|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"|S134
-|align="center"|MP135
-|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - gfp Tripart
-|align="center"|D105 (BV) - D131 (BI)
-|
-|align="center"|[[Team:Paris/August 1|August 1]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L121
+| style="background: #D4E2EF;" |L
-|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+|align="center"|pCR4Blunt-TOPO
-|align="center"|S138
+|align="center"|S181
-|align="center"|MP139
+|align="center"|MP178
-|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pTet - gfp tripart
+|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFliL-RBS (WT)
-|align="center"|D106 (BV) - D131 (BI)<br>In Top10 strain
+|align="center"|
-|
+|align="center"|OK sequencing
-|align="center"|[[Team:Paris/August 1|August 1]]
+|align="center"|[[Team:Paris/September 04|September 04]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L121
+| style="background: #D4E2EF;" |L
-|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+|align="center"|pCR4Blunt-TOPO
-|align="center"|S171
+|align="center"|S180
-|align="center"|not done
+|align="center"|MP177
-|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pTet - gfp Tripart
+|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlhDC-RBS (WT)
-|align="center"|D106 (BV) - D131 (BI)
+|
-|align="center"|Same as S138 but in chemically labmade competent MG1655 bacteria. For study of doubling time of  population.
+|align="center"|OK sequencing
-|align="center" |[[Team:Paris/August 22|Aug 22]]
+|align="center"|[[Team:Paris/September 04|September 04]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L156
+| style="background: #D4E2EF;" |L
+|align="center"|pCR4Blunt-TOPO
+|align="center"|S182
+|align="center"|MP179
+|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>RBS(FlhDC) - FlhDC
+|
+|align="center"|FlhDC with conserved Pst1 site
+|align="center"|[[Team:Paris/September 04|September 04]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |L
+|align="center"|pCR4Blunt-TOPO
+|align="center"|S183
+|align="center"|MP180
+|align="center"|[[Image:Icon_coding.png]]<br> FliA2
 |align="center"|
+|align="center"|mutated at EcoR1 and Pst1 internal site
+|align="center"|[[Team:Paris/September 04|September 04]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |L
+|align="center"|pCR4Blunt-TOPO
+|align="center"|S184
+|align="center"|MP181
+|align="center"|[[Image:Icon_coding.png]]<br> FliA3
 |align="center"|
+|align="center"|FliA mutated at EcoR1 and Pst1 site
+|align="center"|[[Team:Paris/September 04|September 04]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |L
+|align="center"|pCR4Blunt-TOPO
+|align="center"|S185
+|align="center"|MP182
+|align="center"|[[Image:Icon_coding.png]]<br> EnvZ*
 |align="center"|
-|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pTet-gfp generator
+|align="center"|the good one but not mutated for EcoR1 site
-|align="center"|D161 (FV) - D163 (FI)
+|align="center"|[[Team:Paris/September 04|September 04]]
-|
-|align="center"|[[Team:Paris/August 22|August 22]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L122
+| style="background: #D4E2EF;" |L
-|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+|align="center"|pCR4Blunt-TOPO
-|align="center"|S150
+|align="center"|S186
-|align="center"|MP151
+|align="center"|MP183
-|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS-lasI - ECFP
+|align="center"|[[Image:Icon_coding.png]]<br> OmpR WT
-|align="center"|D107 (BV) - D130 (BI)
+|align="center"|
-|
+|align="center"|OmpR WT not the mutated
-|align="center"|[[Team:Paris/August 13|August 13]]
+|align="center"|[[Team:Paris/September 04|September 04]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L123
+| style="background: #D4E2EF;" |pFlgB
-|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+|align="center"|pCR4Blunt-TOPO
-|align="center"|S151
+|align="center"|S187
-|align="center"|MP152
+|align="center"|MP184
-|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS lasI - gfp tripart
+|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlgB-RBS (WT)
-|align="center"|D107 (BV) - D131 (BI)
+|
-|
+|align="center"|OK sequencing
-|align="center"|[[Team:Paris/August 13|August 13]]
+|align="center"|[[Team:Paris/September 04|September 04]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L163
+| style="background: #D4E2EF;" |L
-|align="center"|[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]
+|align="center"|pCR4Blunt-TOPO
-|align="center"| x
+|align="center"|S188
-|align="center"| x
+|align="center"|MP185
-|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> rbs-lasI-gfp generator
+|align="center"|[[Image:Icon_coding.png]]<br> OmpR WT
-|align="center"|D107 (BV) - D163 (BI)
 |align="center"|
-|align="center"|[[Team:Paris/August 22|August 22]]
+|align="center"|OmpR WT not the mutated
+|align="center"|[[Team:Paris/September 04|September 04]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L100
+| style="background: #D4E2EF;" |L179
-|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+|align="center"|[http://partsregistry.org/Part:J61002 J61002]
-|align="center"|S146
+|align="center"|S200
-|align="center"|MP147
+|align="center"|MP200
-|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>rbs TetR - ECFP
+|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>J23101 + RBS (B0032) + tet R
-|align="center"|D110 (BV) -  D130 (BI)
 |
-|align="center"|[[Team:Paris/August 13|August 13]]
+|
+|align="center"|[[Team:Paris/September 24|September 24]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L101
+| style="background: #D4E2EF;" |L
-|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+|align="center"|[http://partsregistry.org/Part:J61002 J61002]
-|align="center"|S147
+|align="center"|S201
-|align="center"|MP148
+|align="center"|MP201
-|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>rbs TetR - GFP tripart
+|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlgA(RBS WT)-EnvZ*
-|align="center"|D110 (BV) -  D131 (BI)
+|
-|align="center"|DO NOT WORK !!!!
+|align="center"|probably wrong according to MP208 and MP209 that have been sequenced
-|align="center"|[[Team:Paris/August 13|August 13]]
+|align="center"|[[Team:Paris/September 24|September 24]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L158
+| style="background: #D4E2EF;" |L198
-|align="center"|
+|align="center"|[http://partsregistry.org/Part:J61002 J61002]
-|align="center"| x
+|align="center"|S207
-|align="center"| x
+|align="center"|MP207
-|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> rbs-tetR-gfp generator
+|align="center"|[[Image:Icon_regulatory.png]]<br>pFliA
-|align="center"|D110(BV) - D131 (BI)
+|
-|align="center"|didn't work
+|align="center"|OK sequencing
-|align="center"|[[Team:Paris/August 23|August 23]]
+|align="center"|[[Team:Paris/October 04|October 04]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L162
+| style="background: #D4E2EF;" |L203
-|align="center"|[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]
+|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
-|align="center"| x
+|align="center"|S208
-|align="center"| x
+|align="center"|MP208
-|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> rbs-TetR-gfp generator
+|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br> pFlgA(RBS WT)-EnvZ*
-|align="center"|D110 (BV) - D163 (BI)
+|
-|align="center"|
+|align="center"|in fact lack of pFlgA
-|align="center"|[[Team:Paris/August 22|August 22]]
+|align="center"|[[Team:Paris/October 04|October 04]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L150
+| style="background: #D4E2EF;" |L206
-|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-|align="center"| x
-|align="center"|MP161
-|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas + rbs-tetR-gfp tripart
-|align="center"|D105 (BV) - D146 (BI)
-|incorrect clone <br>-> to do again
-|align="center"|[[Team:Paris/August 19|August 19]]
-|- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L
 |align="center"|[http://partsregistry.org/Part:J61002 J61002]
 |align="center"|S209
@@ Line 1,479: / Line 1,127: @@
 |align="center"|in fact lack of pFlgA
 |align="center"|[[Team:Paris/October 04|October 04]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L176
+| style="background: #D4E2EF;" |L209
-|align="center"|
+|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
-|align="center"|
+|align="center"|S210
-|align="center"|
+|align="center"|MP210
-|align="center"|
+|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFliL(RBS WT)
-|align="center"|D125 (v) + D162 (i)
+|
-|
+|align="center"|OK sequencing
-|align="center"|[[Team:Paris/September |September ]]
+|align="center"|[[Team:Paris/October 10|October 10]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L180
+| style="background: #D4E2EF;" |L
-|align="center"|
+|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
-|align="center"|
+|align="center"|S211
-|align="center"|
+|align="center"|MP211
-|align="center"|
+|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlgA(RBS WT)
-|align="center"|D166 (v) + D109.2 (i)
+|
-|
+|align="center"|OK sequencing
-|align="center"|[[Team:Paris/September |September ]]
+|align="center"|[[Team:Paris/October 10|October 10]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L181
+| style="background: #D4E2EF;" |L208
-|align="center"|
+|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
-|align="center"|
+|align="center"|S212
-|align="center"|
+|align="center"|MP212
-|align="center"|
+|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlhB(RBS WT)
-|align="center"|D199 (v) + D109.2 (i)
+|
-|
+|align="center"|OK sequencing
-|align="center"|[[Team:Paris/September |September ]]
+|align="center"|[[Team:Paris/October 10|October 10]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L182
+| style="background: #D4E2EF;" |L197
-|align="center"|
+|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002]
-|align="center"|
+|align="center"|S213
-|align="center"|
+|align="center"|MP213
-|align="center"|
+|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlhB(RBS WT)
-|align="center"|D187 (v) + D109.2 (i)
 |
-|align="center"|[[Team:Paris/September |September ]]
+|align="center"|OK sequencing
+|align="center"|[[Team:Paris/October 10|October 10]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |L200
+|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002]
+|align="center"|S214
+|align="center"|MP214
+|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFliL(RBS WT)
+|
+|align="center"|OK sequencing
+|align="center"|[[Team:Paris/October 10|October 10]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L186
+| style="background: #D4E2EF;" |[http://partsregistry.org/Part:pSB4T5 pSB4T5]
-|align="center"|
+|align="center"|[http://partsregistry.org/Part:pSB4T5 pSB4T5]
-|align="center"|
+|align="center"|S215
-|align="center"|
+|align="center"|MP215
-|align="center"|
+|align="center"|[[Image:icon_plasmid.png]]<br>plasmid
-|align="center"|D179 (v) + D205 (i)
 |
-|align="center"|[[Team:Paris/September |September ]]
+|align="center"|low copy plasmid with pSC101 ORI
+|align="center"|[[Team:Paris/October 14|October 14]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L190 ("L4")
+| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_E0434 E0434]
-|align="center"|
+|align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
-|align="center"|
+|align="center"|S216
-|align="center"|
+|align="center"|MP216
-|align="center"|
+|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>YFP tripart +AAV
-|align="center"|D199 (v) + D (i)
 |
-|align="center"|[[Team:Paris/September |September ]]
+|align="center"|Fluorescence OK
+|align="center"|[[Team:Paris/October 15|October 15]]
 |- style="background: #dddddd;"
-| style="background: #D4E2EF;" |L195 ("L9")
+| style="background: #D4E2EF;" |JM45
-|align="center"|
+|align="center"|pJM45
-|align="center"|
+|align="center"|S217
-|align="center"|
+|align="center"|MP217
-|align="center"|
+|align="center"|[[Image:Icon_plasmid.png]]<br>plasmid
-|align="center"|D187 (v) + D194 (i)
 |
-|align="center"|[[Team:Paris/September |September ]]
+|align="center"|inducible FlhDC in pBad18 (ORI pBR / ampicillin resistance)
+|align="center"|[[Team:Paris/October 24|October 24]]
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |L226
@@ Line 1,606: / Line 1,259: @@
 |align="center"|
 |align="center"|[[Team:Paris/October 24|October 24]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |L225
+|align="center"|[http://partsregistry.org/Part:J61002 J61002]
+|align="center"|S224
+|align="center"|MP224
+|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFliL(RBS WT)-EnvZ*
+|
+|align="center"|
+|align="center"|[[Team:Paris/October 26|October 26]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |L233
+|align="center"|[http://partsregistry.org/Part:J61002 J61002]
+|align="center"|S225
+|align="center"|MP225
+|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlhB(RBS WT)-GFP
+|
+|align="center"|
+|align="center"|[[Team:Paris/October 26|October 26]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |U317
+|align="center"|pU317
+|align="center"|S226
+|align="center"|MP226
+|align="center"|[[Image:Icon_plasmid.png]]<br>plasmid
+|
+|align="center"|inducible FliA in pBad18 (ORI pBR  / ampicillin resistance)
+|align="center"|[[Team:Paris/October 23|October 23]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |NEC283
+|align="center"|Strain
+|align="center"|S227
+|align="center"|
+|align="center"|Strain MG1655
+|
+|align="center"|Δ FliA Frt
+|align="center"|[[Team:Paris/October 25|October 25]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |NEC284
+|align="center"|Strain
+|align="center"|S228
+|align="center"|
+|align="center"|Strain MG1655
+|
+|align="center"|Δ FlhD Frt
+|align="center"|[[Team:Paris/October 25|October 25]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |NEC285
+|align="center"|Strain
+|align="center"|S229
+|align="center"|
+|align="center"|Strain MG1655
+|
+|align="center"|Δ FlgM Frt
+|align="center"|[[Team:Paris/October 25|October 25]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |Δ EnvZ :: Cm
+|align="center"|Strain
+|align="center"|S230
+|align="center"|
+|align="center"|Strain MG1655
+|
+|align="center"|Δ EnvZ :: Cm
+|align="center"|[[Team:Paris/October 25|October 25]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |L216
+|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
+|align="center"|MP231
+|align="center"|S231
+|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*
+|
+|align="center"|inducible EnvZ*
+|align="center"|[[Team:Paris/October 27|October 27]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |L236
+|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002 ]
+|align="center"|MP232
+|align="center"|S232
+|align="center"|[[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+WT RBS)-EnvZ*-GFP generator
+|
+|align="center"|
+|align="center"|[[Team:Paris/October 29|October 29]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |L235
+|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002 ]
+|align="center"|MP233
+|align="center"|S233
+|align="center"|[[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+WT RBS)-EnvZ*-GFP generator
+|
+|align="center"|
+|align="center"|[[Team:Paris/October 29|October 29]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_J23100 J23100]
+|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002]
+|align="center"|MP1-2007
+|align="center"|MP116
+|align="center"|[[Image:Part_icon_regulatory.png]]<br>Strong constitutive promoter
+|
+|
+|align="center"|[[Team:Paris/July 26|July 26]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_J23107 J23107]
+|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002]
+|align="center"|MP2-2007
+|align="center"|MP117
+|align="center"|[[Image:Part_icon_regulatory.png]]<br>Medium constitutive promoter
+|
+|
+|align="center"|[[Team:Paris/July 24|July 24]], [[Team:Paris/July 26|July 26]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_B0030 B0030]
+|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+|align="center"|MP5-2007
+|align="center"|MP120
+|align="center"|[[Image:Part_icon_rbs.png]]<br>Strong RBS (Efficiency = 0,6)
+|
+|
+|align="center"|[[Team:Paris/July 26|July 26]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_B0015 B0015]
+|align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
+|align="center"|MP3-2007
+|align="center"|MP118
+|align="center"|[[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Double terminator
+|
+|align="center"|
+|align="center"|[[Team:Paris/July 24|July 24]], [[Team:Paris/July 26|July 26]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |L174
+|align="center"|[http://partsregistry.org/Part:J61002 J61002]
+|align="center"|S
+|align="center"|MP202
+|align="center"|<br>[[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>Strong constitutive promoter-GFP generator
+|align="center"|J23101-E0240
+|
+|align="center"|[[Team:Paris/September 26|September 26]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |L157
+|align="center"|[http://partsregistry.org/Part:pSB1K3 pSB1AK3]
+|align="center"|
+|align="center"|
+|align="center"|[[Image:Icon_coding.png]][[Image:Icon_rbs.png]]<br> tetR-rbs (B0015)
+|align="center"|D125 (BV) - D162 (BI)
+|
+|align="center"|[[Team:Paris/August 23|August 23]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |L155
+|align="center"|[http://partsregistry.org/Part:J61002 J61002]
+|align="center"|
+|align="center"|
+|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> strongest promoter-gfp generator
+|align="center"|D164(FV) - D163 (FI)
+|
+|align="center"|[[Team:Paris/August 22|August 22]]
+|- style="background: #dddddd;"
+| style="background: #D4E2EF;" |L156
+|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+|align="center"|
+|align="center"|
+|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pTet-gfp generator
+|align="center"|D161 (FV) - D163 (FI)
+|
+|align="center"|[[Team:Paris/August 22|August 22]]
 |}