Team:Paris/Notebook/Freezer

From 2008.igem.org

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Line 383: Line 383:
|align="center"|S103
|align="center"|S103
|align="center"|MP119
|align="center"|MP119
-
|align="center"|[[Image:Part_icon_regulatory.png]]<br>AraC pBAD
+
|align="center"|[[Image:Part_icon_regulatory.png]]<br>araC/pBad
|
|
|
|
Line 392: Line 392:
|align="center"|S106
|align="center"|S106
|align="center"|MP122
|align="center"|MP122
-
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>gfp tri-part; strong rbs
+
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>GFP generator; strong rbs
|
|
|align="center"|
|align="center"|
Line 410: Line 410:
|align="center"|S108
|align="center"|S108
|align="center"|MP103
|align="center"|MP103
-
|align="center"|[[Image:Part_icon_regulatory.png]]<br>promoter pLas (lasr & PAI regulated)
+
|align="center"|[[Image:Part_icon_regulatory.png]]<br>pLas (lasr & PAI regulated)
|
|
|
|
Line 437: Line 437:
|align="center"|S111
|align="center"|S111
|align="center"|MP104
|align="center"|MP104
-
|align="center"|[[Image:Part_icon_regulatory.png]]<br>tetR repressible promoter
+
|align="center"|[[Image:Part_icon_regulatory.png]]<br>pTetR repressible
|
|
|
|
Line 584: Line 584:
|align="center"|S127
|align="center"|S127
|align="center"|MP128
|align="center"|MP128
-
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Strong promoter - gfp Tripart
+
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Strong promoter - GFP Tripart
|align="center"|D123 (BV) - D131 (BI)
|align="center"|D123 (BV) - D131 (BI)
|
|
Line 603: Line 603:
|align="center"|S129
|align="center"|S129
|align="center"|MP130
|align="center"|MP130
-
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Strongest promoter -  gfp Tripart
+
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Strongest promoter -  GFP Tripart
|align="center"|D103 (BV) - D131 (BI)
|align="center"|D103 (BV) - D131 (BI)
|
|
Line 612: Line 612:
|align="center"|S130
|align="center"|S130
|align="center"|MP131
|align="center"|MP131
-
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Medium promoter -  gfp Tripart
+
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Medium promoter -  GFP Tripart
|align="center"|D124 (BV) - D131 (BI)
|align="center"|D124 (BV) - D131 (BI)
|
|
Line 657: Line 657:
|align="center"|S135
|align="center"|S135
|align="center"|MP136
|align="center"|MP136
-
|align="center"|[[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>yfp - Double Terminator
+
|align="center"|[[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>YFP - Double Terminator
|align="center"|D125 (FV) - D117 (FI)
|align="center"|D125 (FV) - D117 (FI)
|
|
Line 666: Line 666:
|align="center"|S136
|align="center"|S136
|align="center"|MP137
|align="center"|MP137
-
|align="center"|[[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>rfp - Double Terminator
+
|align="center"|[[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>mRFP - Double Terminator
|align="center"|D125 (FV) - D121 (FI)
|align="center"|D125 (FV) - D121 (FI)
|
|
Line 684: Line 684:
|align="center"|S138
|align="center"|S138
|align="center"|MP139
|align="center"|MP139
-
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pTet - gfp tripart
+
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pTet - GFP  tripart
|align="center"|D106 (BV) - D131 (BI)<br>In Top10 strain
|align="center"|D106 (BV) - D131 (BI)<br>In Top10 strain
|
|
Line 693: Line 693:
|align="center"|S139
|align="center"|S139
|align="center"|MP140
|align="center"|MP140
-
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]<br>Strongest RBS - rfp
+
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]<br>Strongest RBS - mRFP
|align="center"|D102 (BV) - D122 (BI)
|align="center"|D102 (BV) - D122 (BI)
|
|
Line 702: Line 702:
|align="center"|S140
|align="center"|S140
|align="center"|MP141
|align="center"|MP141
-
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]<br>Strongest RBS - yfp
+
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]<br>Strongest RBS - YFP
|align="center"|D102 (BV) - D118 (BI)
|align="center"|D102 (BV) - D118 (BI)
|
|
Line 749: Line 749:
|align="center"|S148
|align="center"|S148
|align="center"|MP149
|align="center"|MP149
-
|align="center"| [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>AracpBAD - gfp tripart
+
|align="center"| [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>araC/pBad - GFP tripart
|align="center"|D126 (BV) - D131 (BI)
|align="center"|D126 (BV) - D131 (BI)
|
|
Line 776: Line 776:
|align="center"|S151
|align="center"|S151
|align="center"|MP152
|align="center"|MP152
-
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS lasI - gfp tripart
+
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS lasI - GFP tripart
|align="center"|D107 (BV) - D131 (BI)
|align="center"|D107 (BV) - D131 (BI)
|
|
Line 821: Line 821:
|align="center"|S156
|align="center"|S156
|align="center"|MP157
|align="center"|MP157
-
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>gfp generator (E0240)
+
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>GFP generator (E0240)
|align="center"| D137(FV) - D138(FI)
|align="center"| D137(FV) - D138(FI)
|
|
Line 844: Line 844:
|align="center"|[[Team:Paris/August 15|August 15]]
|align="center"|[[Team:Paris/August 15|August 15]]
-
|- style="background: #dddddd;" 
 
-
| style="background: #D4E2EF;" |L139
 
-
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
 
-
|align="center"|S159
 
-
|align="center"|MP158
 
-
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>E0240 RBS+
 
-
|align="center"|D140 (PstI) - D144 (ES)
 
-
|
 
-
|align="center"|[[Team:Paris/August 15|August 15]]
 
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |L141
| style="background: #D4E2EF;" |L141
Line 863: Line 854:
|align="center"|[[Team:Paris/August 15|August 15]]
|align="center"|[[Team:Paris/August 15|August 15]]
-
|- style="background: #dddddd;" 
 
-
| style="background: #D4E2EF;" |L142
 
-
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
 
-
|align="center"|S161
 
-
|align="center"|MP160
 
-
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>flhDC+promotor
 
-
|align="center"| D143 (ES) - D145 (ES)
 
-
|align="center"|does not have <br> the insert
 
-
|align="center"|[[Team:Paris/August 15|August 15]]
 
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |L151
| style="background: #D4E2EF;" |L151
Line 1,006: Line 988:
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlhB-RBS (WT)
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlhB-RBS (WT)
|  
|  
-
|  
+
|align="center"|OK sequencing
|align="center"|[[Team:Paris/September 04|September 04]]
|align="center"|[[Team:Paris/September 04|September 04]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 1,014: Line 996:
|align="center"|MP176
|align="center"|MP176
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFliA-RBS (WT)  
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFliA-RBS (WT)  
-
|  
+
|align="center"|
|align="center"|pFliA with conserved Pst1 site
|align="center"|pFliA with conserved Pst1 site
|align="center"|[[Team:Paris/September 04|September 04]]
|align="center"|[[Team:Paris/September 04|September 04]]
Line 1,024: Line 1,006:
|align="center"|MP178
|align="center"|MP178
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFliL-RBS (WT)
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFliL-RBS (WT)
-
|  
+
|align="center"|
-
|  
+
|align="center"|OK sequencing
|align="center"|[[Team:Paris/September 04|September 04]]
|align="center"|[[Team:Paris/September 04|September 04]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 1,034: Line 1,016:
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlhDC-RBS (WT)
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlhDC-RBS (WT)
|  
|  
-
|  
+
|align="center"|OK sequencing
|align="center"|[[Team:Paris/September 04|September 04]]
|align="center"|[[Team:Paris/September 04|September 04]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 1,088: Line 1,070:
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlgB-RBS (WT)
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlgB-RBS (WT)
|  
|  
-
|  
+
|align="center"|OK sequencing
|align="center"|[[Team:Paris/September 04|September 04]]
|align="center"|[[Team:Paris/September 04|September 04]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 1,119: Line 1,101:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |L
+
| style="background: #D4E2EF;" |L198
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|S207
|align="center"|S207
Line 1,125: Line 1,107:
|align="center"|[[Image:Icon_regulatory.png]]<br>pFliA
|align="center"|[[Image:Icon_regulatory.png]]<br>pFliA
|
|
-
|
+
|align="center"|OK sequencing
|align="center"|[[Team:Paris/October 04|October 04]]
|align="center"|[[Team:Paris/October 04|October 04]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |L
+
| style="background: #D4E2EF;" |L203
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
|align="center"|S208
|align="center"|S208
Line 1,137: Line 1,119:
|align="center"|[[Team:Paris/October 04|October 04]]
|align="center"|[[Team:Paris/October 04|October 04]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |L
+
| style="background: #D4E2EF;" |L206
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|S209
|align="center"|S209
Line 1,146: Line 1,128:
|align="center"|[[Team:Paris/October 04|October 04]]
|align="center"|[[Team:Paris/October 04|October 04]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |L
+
| style="background: #D4E2EF;" |L209
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
|align="center"|S210
|align="center"|S210
Line 1,152: Line 1,134:
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFliL(RBS WT)
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFliL(RBS WT)
|  
|  
-
|  
+
|align="center"|OK sequencing
|align="center"|[[Team:Paris/October 10|October 10]]
|align="center"|[[Team:Paris/October 10|October 10]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 1,161: Line 1,143:
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlgA(RBS WT)
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlgA(RBS WT)
|  
|  
-
|
+
|align="center"|OK sequencing
|align="center"|[[Team:Paris/October 10|October 10]]
|align="center"|[[Team:Paris/October 10|October 10]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |L
+
| style="background: #D4E2EF;" |L208
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
|align="center"|S212
|align="center"|S212
Line 1,170: Line 1,152:
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlhB(RBS WT)
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlhB(RBS WT)
|  
|  
-
|  
+
|align="center"|OK sequencing
|align="center"|[[Team:Paris/October 10|October 10]]
|align="center"|[[Team:Paris/October 10|October 10]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |L
+
| style="background: #D4E2EF;" |L197
|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002]
|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002]
|align="center"|S213
|align="center"|S213
Line 1,179: Line 1,161:
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlhB(RBS WT)
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlhB(RBS WT)
|
|
-
|  
+
|align="center"|OK sequencing
|align="center"|[[Team:Paris/October 10|October 10]]
|align="center"|[[Team:Paris/October 10|October 10]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |L
+
| style="background: #D4E2EF;" |L200
|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002]
|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002]
|align="center"|S214
|align="center"|S214
Line 1,188: Line 1,170:
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFliL(RBS WT)
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFliL(RBS WT)
|  
|  
-
|  
+
|align="center"|OK sequencing
|align="center"|[[Team:Paris/October 10|October 10]]
|align="center"|[[Team:Paris/October 10|October 10]]
Line 1,207: Line 1,189:
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>YFP tripart +AAV
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>YFP tripart +AAV
|
|
-
|align="center"|
+
|align="center"|Fluorescence OK
|align="center"|[[Team:Paris/October 15|October 15]]
|align="center"|[[Team:Paris/October 15|October 15]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 1,216: Line 1,198:
|align="center"|[[Image:Icon_plasmid.png]]<br>plasmid
|align="center"|[[Image:Icon_plasmid.png]]<br>plasmid
|
|
-
|align="center"|FlhDC in pBad18 (ORI pBR / ampicillin resistance)
+
|align="center"|inducible FlhDC in pBad18 (ORI pBR / ampicillin resistance)
|align="center"|[[Team:Paris/October 24|October 24]]
|align="center"|[[Team:Paris/October 24|October 24]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 1,302: Line 1,284:
|align="center"|[[Image:Icon_plasmid.png]]<br>plasmid
|align="center"|[[Image:Icon_plasmid.png]]<br>plasmid
|
|
-
|align="center"|FliA in pBad18 (ORI pBR  / ampicillin resistance)
+
|align="center"|inducible FliA in pBad18 (ORI pBR  / ampicillin resistance)
|align="center"|[[Team:Paris/October 23|October 23]]
|align="center"|[[Team:Paris/October 23|October 23]]
Line 1,342: Line 1,324:
|align="center"|Δ EnvZ :: Cm
|align="center"|Δ EnvZ :: Cm
|align="center"|[[Team:Paris/October 25|October 25]]
|align="center"|[[Team:Paris/October 25|October 25]]
 +
|- style="background: #dddddd;"
 +
| style="background: #D4E2EF;" |L216
 +
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
 +
|align="center"|MP231
 +
|align="center"|S231
 +
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*
 +
|
 +
|align="center"|inducible EnvZ*
 +
|align="center"|[[Team:Paris/October 27|October 27]]
 +
|- style="background: #dddddd;"
 +
| style="background: #D4E2EF;" |L236
 +
|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002 ]
 +
|align="center"|MP232
 +
|align="center"|S232
 +
|align="center"|[[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+WT RBS)-EnvZ*-GFP generator
 +
|
 +
|align="center"|
 +
|align="center"|[[Team:Paris/October 29|October 29]]
 +
|- style="background: #dddddd;"
 +
| style="background: #D4E2EF;" |L235
 +
|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002 ]
 +
|align="center"|MP233
 +
|align="center"|S233
 +
|align="center"|[[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+WT RBS)-EnvZ*-GFP generator
 +
|
 +
|align="center"|
 +
|align="center"|[[Team:Paris/October 29|October 29]]
 +
Line 1,351: Line 1,361:
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_J23100 J23100]
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_J23100 J23100]
|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002]  
|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002]  
-
|align="center"|x
+
|align="center"|MP1-2007
|align="center"|MP116
|align="center"|MP116
|align="center"|[[Image:Part_icon_regulatory.png]]<br>Strong constitutive promoter
|align="center"|[[Image:Part_icon_regulatory.png]]<br>Strong constitutive promoter
Line 1,361: Line 1,371:
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_J23107 J23107]
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_J23107 J23107]
|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002]
|align="center"|[http://partsregistry.org/Part:BBa_J61002 J61002]
-
|align="center"|x
+
|align="center"|MP2-2007
|align="center"|MP117
|align="center"|MP117
|align="center"|[[Image:Part_icon_regulatory.png]]<br>Medium constitutive promoter
|align="center"|[[Image:Part_icon_regulatory.png]]<br>Medium constitutive promoter
Line 1,370: Line 1,380:
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_B0030 B0030]
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_B0030 B0030]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-
|align="center"|x
+
|align="center"|MP5-2007
|align="center"|MP120
|align="center"|MP120
|align="center"|[[Image:Part_icon_rbs.png]]<br>Strong RBS (Efficiency = 0,6)
|align="center"|[[Image:Part_icon_rbs.png]]<br>Strong RBS (Efficiency = 0,6)
Line 1,376: Line 1,386:
|  
|  
|align="center"|[[Team:Paris/July 26|July 26]]
|align="center"|[[Team:Paris/July 26|July 26]]
-
|- style="background: #dddddd;" 
 
-
| style="background: #D4E2EF;" |L140
 
-
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|[[Image:Icon_coding.png]]<br>flhD
 
-
|align="center"|D145 (V) - D141 (I)
 
-
|align="center"|
 
-
|align="center"|[[Team:Paris/August 12|August 12]]
 
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_B0015 B0015]
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_B0015 B0015]
|align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
|align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
-
|align="center"|x
+
|align="center"|MP3-2007
|align="center"|MP118
|align="center"|MP118
|align="center"|[[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Double terminator
|align="center"|[[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Double terminator
Line 1,407: Line 1,408:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |L157
| style="background: #D4E2EF;" |L157
-
|align="center"|
+
|align="center"|[http://partsregistry.org/Part:pSB1K3 pSB1AK3]
|align="center"|
|align="center"|
|align="center"|
|align="center"|
Line 1,416: Line 1,417:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |L155
| style="background: #D4E2EF;" |L155
-
|align="center"|
+
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|
|align="center"|
|align="center"|
|align="center"|
Line 1,425: Line 1,426:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |L156
| style="background: #D4E2EF;" |L156
-
|align="center"|
+
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|
|align="center"|
|align="center"|
|align="center"|

Latest revision as of 14:46, 29 October 2008

Contents

Last number used

Miniprep Strain Ligation Digestion PCR
216 216 234 202 154

(last checking on 19th october)

Freezer

PCR products

PCR products
Number Name Oligo F Oligo R Template Length Comments Product Left ? [DNA]µg/mL Date
PCR124 pFlgA O100 O101 - 261pb yes 0.5 August 7
PCR125 pFlgB O102 O103 - 261pb yes 0.5 August 7
PCR126 pFlhB O108 O109 - 260pb yes 0.5 August 7
PCR127 FlhDC O132 O131 MG1655 no August 8
PCR128 OmpR* O138 O139 Strain OmpR* 700pb yes 150 August 8
PCR129 EnvZ* O126 O127 Strain EnvZ* 1400pb yes 100 August 8
PCR130 E0240 rbs+ O141 O140 MP143 900pb no August 11
PCR131 FlhD rbs- O132 O133 MG1655 350pb no August 11
PCR132 pFlhD rbs- O130 O131 MG1655 600pb no August 11
PCR133 FlhDC + prom O110 O131 MG1655 1300pb no August 11
PCR134 FlhDC + prom O111 O131 MG1655 no August 11
PCR135 pFliL O124 O125 MG1655 yes August 11
PCR136 pFlhDC O110 O113 MG1655 yes August 11
PCR137 pFlhDC O 111 O 113 MG1655 yes August 11
PCR138 FlhDC (RBS -) O 132 O 131 MG 1655 yes August 15
PCR139 pFlhDC (URI) O 110 O 113 PCR 130 yes August 15
PCR140 FlhDC (RBS -) O 132 O 131 PCR 130 yes August 15
PCR141 Gene FlhDC + rbs nat O 134 O 131 MG1655 1000pb yes August 26
PCR142 pFliA + rbs nat O 145 O 144 MG1655 325pb yes August 26
PCR143 pFliA sans rbs O 145 O 146 MG1655 310pb yes August 26
PCR144 pFliA + Gene FliA O 145 O 151 MG1655 1100pb yes August 26
PCR145 FliA1 O 148 O 152 MG1655 150pb yes August 26
PCR146 FliA2 O 153 O 150 MG1655 670pb yes August 26
PCR 147 EnvZ* O 126 O 127 S 120 1412 bp yes 150 August 26
PCR 148 OmpR* O 138 O 139 S 119 789 bp yes 101 August 26
PCR 149 pFlhDC (Soutourina +RBS) O 154 O 113 MG1655 450 bp yes August 26
PCR 151 pFLiA without nat. rbs O 145 O 146 MG1655 310 bp yes 34 August 26
PCR 152 pFliA rbs nat. O 145 O 144 MG1655 325 bp yes 19 August 26
PCR 153 pFliA+Gene FliA non-mutated O 145 O 151 MG1655 1100 bp yes 39 August 26
PCR 154 Gene FliA mutated (EcoRI/PstI) O 148 O 150 MG1655 800 bp 154.1, 154.2, 154.3 yes 50 August 26

Parts

Ligations: Constructions realised
Name/N° Plasmid Strain Miniprep Description Digestion comment Date
[http://partsregistry.org/Part:BBa_I0500 I0500] [http://partsregistry.org/Part:pSB2K3 pSB2K3] S103 MP119 Part icon regulatory.png
araC/pBad 		July 24, July 26
[http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S106 MP122 Part icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
GFP generator; strong rbs 		July 26
[http://partsregistry.org/Part:BBa_B0034 B0034 ] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S107 MP100 Part icon rbs.png
Strongest RBS (Efficiency = 1) 		July 26
[http://partsregistry.org/Part:BBa_R0079 R0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S108 MP103 Part icon regulatory.png
pLas (lasr & PAI regulated) 		July 23, July 26
[http://partsregistry.org/Part:BBa_J23101 J23101] [http://partsregistry.org/Part:BBa_J61002 J61002 ] S109 MP101 Part icon regulatory.png
Strongest Constitutive promoter 		July 22, July 23, July 26
[http://partsregistry.org/Part:BBa_J23109 J23109] [http://partsregistry.org/Part:BBa_J61002 J61002] S110 MP102 Part icon regulatory.png
Weak Constitutive promoter 		July 22, July 26
[http://partsregistry.org/Part:BBa_R0040 R0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S111 MP104 Part icon regulatory.png
pTetR repressible 		July 23, July 26
[http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S112 MP105 Part icon rbs.pngIcon coding.png
B0034(rbs) ->LasI 		July 26
[http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S113 MP106 Part icon rbs.pngIcon coding.png
B0034(rbs) -> TetR 		August 24
[http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] S114 MP107 Icon coding.png
lasR activator with LVA Tag 		July 23
[http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S115 MP108 Icon coding.png
lasR activator 		July 22, July 23, July 26
[http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] S116 MP109 Part icon reporter.png
YFP 		July 26
[http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S117 MP110 Part icon reporter.png
GFP 		July 23, July 26
[http://partsregistry.org/Part:BBa_E1010 E1010 ] [http://partsregistry.org/Part:pSB2K3 pSB2K3] S118 MP111 Part icon reporter.png
mRFP 		July 23, July 26
OmpR* M57I Strain S119 Strain MG1655
Icon coding.png
OmpR* 		Really mutated? July 23
EnvZ* I281S Strain S120 Strain MG1655
Icon coding.png
EnvZ* 		July 23
[http://partsregistry.org/Part:BBa_C0040 C0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S121 MP114 Icon coding.png
tetR repressor 		July 23
[http://partsregistry.org/Part:pSB1A3 pSB1A3] [http://partsregistry.org/Part:pSB1A3 pSB1A3] S122 MP115 Icon plasmid.png
plasmid 		July 23
L102 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S123 MP124 Part icon rbs.pngPart icon reporter.png
Strong rbs - YFP 		D129 (BV) - D118 (BI) August 1
L103 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S124 MP125 Part icon rbs.pngPart icon reporter.png
Strong rbs - mRFP 		D129 (BV) - D122 (BI) August 1
L104 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S125 MP126 Part icon rbs.pngIcon coding.png
Strong rbs - lasR activator 		D129 (BV) - D114 (BI) August 1
L105 [http://partsregistry.org/Part:J61002 J61002] S126 MP127 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
Strong promoter - ECFP 		D123 (BV) - D130 (BI) August 1
L106 [http://partsregistry.org/Part:J61002 J61002] S127 MP128 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
Strong promoter - GFP Tripart 		D123 (BV) - D131 (BI) August 1
L107 [http://partsregistry.org/Part:J61002 J61002] S128 MP129 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
Strongest promoter - ECFP 		D103 (BV) - D130 (BI) August 1
L108 [http://partsregistry.org/Part:J61002 J61002] S129 MP130 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
Strongest promoter - GFP Tripart 		D103 (BV) - D131 (BI) August 1
L110 [http://partsregistry.org/Part:J61002 J61002] S130 MP131 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
Medium promoter - GFP Tripart 		D124 (BV) - D131 (BI) August 1
L111 [http://partsregistry.org/Part:J61002 J61002] S131 MP132 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
Weak promoter - ECFP 		D104 (BV) - D130 (BI) August 1
L112 [http://partsregistry.org/Part:J61002 J61002] S132 MP133 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
Weak promoter - GFP tripart 		D104 (BV) - D131 (BI) August 1
L115 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S133 MP134 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pLas - ECFP 		D105 (BV) - D130 (BI) August 1
L116 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S134 MP135 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pLas - gfp Tripart 		D105 (BV) - D131 (BI) August 1
L117 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] S135 MP136 Part icon reporter.pngPart icon terminator.pngPart icon terminator.png
YFP - Double Terminator 		D125 (FV) - D117 (FI) August 1
L118 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] S136 MP137 Part icon reporter.pngPart icon terminator.pngPart icon terminator.png
mRFP - Double Terminator 		D125 (FV) - D121 (FI) August 1
L119 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] S137 MP138 Icon coding.pngPart icon terminator.pngPart icon terminator.png
lasR activator + LVA - Double Terminator 		D125 (FV) - D113 (FI) August 1
L121 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S138 MP139 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pTet - GFP tripart 		D106 (BV) - D131 (BI)
In Top10 strain 		August 1
L124 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S139 MP140 Part icon rbs.pngPart icon reporter.png
Strongest RBS - mRFP 		D102 (BV) - D122 (BI) August 1
L125 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S140 MP141 Part icon rbs.pngPart icon reporter.png
Strongest RBS - YFP 		D102 (BV) - D118 (BI) August 1
MG1655 Strain S141 Strain Aug 7
[http://partsregistry.org/Part:BBa_J61002 J61002] [http://partsregistry.org/Part:BBa_J61002 J61002] S142 MP123 Icon plasmid.png
plasmid 		August 5
L128 [http://partsregistry.org/Part:J61002 J61002] S143 MP144 Part icon regulatory.png
pFlgA 		D132 (FI) - D136 (FV) OK sequencing August 12
L100 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S146 MP147 Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
rbs TetR - ECFP 		D110 (BV) - D130 (BI) August 13
L114 [http://partsregistry.org/Part:pSB2K3 pSB2K3] S148 MP149 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
araC/pBad - GFP tripart 		D126 (BV) - D131 (BI) August 13
L120 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S149 MP150 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pTet - ECFP 		D106 (BV) - D130 (BI) August 13
L122 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S150 MP151 Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS-lasI - ECFP 		D107 (BV) - D130 (BI) August 13
L123 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S151 MP152 Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS lasI - GFP tripart 		D107 (BV) - D131 (BI) August 13
L126 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S152 MP153 Part icon rbs.pngIcon coding.png
Strongest RBS (1)- LasR activator (+LVA) 		D102 (BV) - D114 (BI) August 13
L132 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S153 MP154 Icon coding.png
flhDC 		D142(FV) - D139(FI) August 13
L133 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S154 MP155 Icon coding.png
OmpR* 		D142(FV) - D140(FI) WT sequence August 19
L134 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S155 MP156 Icon coding.png
EnvZ* 		D142(FV) - D141(FI) lacks about 300 bp in EnvZ* August 19
L138 [http://partsregistry.org/Part:pSB3K3 pSB3K3] S156 MP157 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
GFP generator (E0240) 		D137(FV) - D138(FI) August 13
[http://partsregistry.org/Part:BBa_E0240 E0240] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S157 MP143 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
GFP generator 		August 15
[http://partsregistry.org/Part:pSB3K3 pSB3K3] [http://partsregistry.org/Part:pSB3K3 pSB3K3] S158 MP142 Icon plasmid.png
plasmid 		low to medium copy plasmid with p15A ORI August 15
L141 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S160 MP159 Icon coding.png
flhC 		D142 (ES) - D145 (ES) has not been sequenced August 15
L151 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] S162 MP162 Icon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png
rbs-lasR + dbl ter 		D147 (FI) - D125 (FV) August 19
[http://partsregistry.org/Part:BBa_B0032 B0032] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S163 MP163 Icon rbs.png
medium rbs (efficiency = 0,3) 		August 20
[http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S164 MP121
MP164 		Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS+ ECFP+ LVA+ term 		July 24, July 26
August 20
[http://partsregistry.org/Part:BBa_E0430 E0430] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S165 MP165 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS+ YFP+ LVA- term 		August 20
[http://partsregistry.org/Part:BBa_E0432 E0432] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S166 MP166 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS+ YFP LVA+ term 		BUT be careful , it doesn't work. See registry August 20
[http://partsregistry.org/Part:BBa_E0420 E0420] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S167 MP167 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS+ ECFP LVA- term 		August 20
[http://partsregistry.org/Part:BBa_I732078 I732078] [http://partsregistry.org/Part:pSB1A3 pSB1A3] S168 MP168 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS+ mRFP LVA+ term 		August 20
L153 [http://partsregistry.org/Part:BBa_J61002 J61002] S169 MP169 Icon regulatory.pngIcon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png
Strong promoter-rbs-lasR-dble ter 		D123 (BV) - D165 (BI) August 20
L154 [http://partsregistry.org/Part:BBa_J61002 J61002] S170 MP170 Icon regulatory.pngIcon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png
strongest promoter-rbs-lasR-dble ter 		D103 (BV) - D165 (BI) August 20
L121 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S171 not done Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pTet - gfp Tripart 		D106 (BV) - D131 (BI) Same as S138 but in chemically labmade competent MG1655 bacteria. For study of doubling time of population. Aug 22
L167 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S172 MP171 Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.pngPart icon regulatory.png
gfp generator(E0240) - pTet 		August 25
L166 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S173 MP172 Part icon rbs.pngIcon coding.png
RBS(B0032) - tetR 		August 25
L160 [http://partsregistry.org/Part:BBa_J61002 J61002] S174 MP173 Icon regulatory.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA - YFP Tripart (LVA-) 		D166 (FV) - D132 (FI) Fluorescence OK August 22
L161 [http://partsregistry.org/Part:BBa_J61002 J61002] S175 MP174 Icon regulatory.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA - YFP Tripart (LVA+) 		D167 (FV) - D132 (FI) August 22
pFlhB pCR4Blunt-TOPO S178 MP175 Icon regulatory.pngIcon rbs.png
pFlhB-RBS (WT) 		OK sequencing September 04
L pCR4Blunt-TOPO S179 MP176 Icon regulatory.pngIcon rbs.png
pFliA-RBS (WT) 		pFliA with conserved Pst1 site September 04
L pCR4Blunt-TOPO S181 MP178 Icon regulatory.pngIcon rbs.png
pFliL-RBS (WT) 		OK sequencing September 04
L pCR4Blunt-TOPO S180 MP177 Icon regulatory.pngIcon rbs.png
pFlhDC-RBS (WT) 		OK sequencing September 04
L pCR4Blunt-TOPO S182 MP179 Part icon rbs.pngIcon coding.png
RBS(FlhDC) - FlhDC 		FlhDC with conserved Pst1 site September 04
L pCR4Blunt-TOPO S183 MP180 Icon coding.png
FliA2 		mutated at EcoR1 and Pst1 internal site September 04
L pCR4Blunt-TOPO S184 MP181 Icon coding.png
FliA3 		FliA mutated at EcoR1 and Pst1 site September 04
L pCR4Blunt-TOPO S185 MP182 Icon coding.png
EnvZ* 		the good one but not mutated for EcoR1 site September 04
L pCR4Blunt-TOPO S186 MP183 Icon coding.png
OmpR WT 		OmpR WT not the mutated September 04
pFlgB pCR4Blunt-TOPO S187 MP184 Icon regulatory.pngIcon rbs.png
pFlgB-RBS (WT) 		OK sequencing September 04
L pCR4Blunt-TOPO S188 MP185 Icon coding.png
OmpR WT 		OmpR WT not the mutated September 04
L179 [http://partsregistry.org/Part:J61002 J61002] S200 MP200 Icon regulatory.pngIcon rbs.pngIcon coding.png
J23101 + RBS (B0032) + tet R 		September 24
L [http://partsregistry.org/Part:J61002 J61002] S201 MP201 Icon regulatory.pngIcon rbs.pngIcon coding.png
pFlgA(RBS WT)-EnvZ* 		probably wrong according to MP208 and MP209 that have been sequenced September 24
L198 [http://partsregistry.org/Part:J61002 J61002] S207 MP207 Icon regulatory.png
pFliA 		OK sequencing October 04
L203 [http://partsregistry.org/Part:pSB3K3 pSB3K3] S208 MP208 Icon regulatory.pngIcon rbs.pngIcon coding.png
pFlgA(RBS WT)-EnvZ* 		in fact lack of pFlgA October 04
L206 [http://partsregistry.org/Part:J61002 J61002] S209 MP209 Icon regulatory.pngIcon rbs.pngIcon coding.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA(RBS WT)-EnvZ*-mRFP tripart LVA+ 		in fact lack of pFlgA October 04
L209 [http://partsregistry.org/Part:pSB3K3 pSB3K3] S210 MP210 Icon regulatory.pngIcon rbs.png
pFliL(RBS WT) 		OK sequencing October 10
L [http://partsregistry.org/Part:pSB3K3 pSB3K3] S211 MP211 Icon regulatory.pngIcon rbs.png
pFlgA(RBS WT) 		OK sequencing October 10
L208 [http://partsregistry.org/Part:pSB3K3 pSB3K3] S212 MP212 Icon regulatory.pngIcon rbs.png
pFlhB(RBS WT) 		OK sequencing October 10
L197 [http://partsregistry.org/Part:BBa_J61002 J61002] S213 MP213 Icon regulatory.pngIcon rbs.png
pFlhB(RBS WT) 		OK sequencing October 10
L200 [http://partsregistry.org/Part:BBa_J61002 J61002] S214 MP214 Icon regulatory.pngIcon rbs.png
pFliL(RBS WT) 		OK sequencing October 10
[http://partsregistry.org/Part:pSB4T5 pSB4T5] [http://partsregistry.org/Part:pSB4T5 pSB4T5] S215 MP215 Icon plasmid.png
plasmid 		low copy plasmid with pSC101 ORI October 14
[http://partsregistry.org/Part:BBa_E0434 E0434] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] S216 MP216 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
YFP tripart +AAV 		Fluorescence OK October 15
JM45 pJM45 S217 MP217 Icon plasmid.png
plasmid 		inducible FlhDC in pBad18 (ORI pBR / ampicillin resistance) October 24
L226 [http://partsregistry.org/Part:J61002 J61002] S218 MP218 Icon regulatory.pngIcon rbs.pngIcon coding.png
pFlhB(RBS WT)-EnvZ* 		October 24
L227 [http://partsregistry.org/Part:J61002 J61002] S219 MP219 Icon regulatory.pngIcon rbs.pngIcon reporter.png
pFliL(RBS WT)-GFP 		October 24
L228 [http://partsregistry.org/Part:J61002 J61002] S220 MP220 Icon regulatory.pngIcon rbs.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFliL(RBS WT)-ECFP tripart (+LVA) 		October 24
L229 [http://partsregistry.org/Part:J61002 J61002] S221 MP221 Icon regulatory.pngIcon rbs.pngIcon reporter.png
pFlgA(RBS WT)-GFP 		October 24
L232 [http://partsregistry.org/Part:J61002 J61002] S222 MP222 Icon regulatory.pngIcon rbs.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA(RBS WT)-YFP tripart (+AAV) 		October 24
L234 [http://partsregistry.org/Part:J61002 J61002] S223 MP223 Icon regulatory.pngIcon rbs.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlhB(RBS WT)-mRFP tripart (+LVA) 		October 24
L225 [http://partsregistry.org/Part:J61002 J61002] S224 MP224 Icon regulatory.pngIcon rbs.pngIcon coding.png
pFliL(RBS WT)-EnvZ* 		October 26
L233 [http://partsregistry.org/Part:J61002 J61002] S225 MP225 Icon regulatory.pngIcon rbs.pngIcon reporter.png
pFlhB(RBS WT)-GFP 		October 26
U317 pU317 S226 MP226 Icon plasmid.png
plasmid 		inducible FliA in pBad18 (ORI pBR / ampicillin resistance) October 23


NEC283 Strain S227 Strain MG1655 Δ FliA Frt October 25
NEC284 Strain S228 Strain MG1655 Δ FlhD Frt October 25
NEC285 Strain S229 Strain MG1655 Δ FlgM Frt October 25
Δ EnvZ :: Cm Strain S230 Strain MG1655 Δ EnvZ :: Cm October 25
L216 [http://partsregistry.org/Part:pSB3K3 pSB3K3] MP231 S231 Icon regulatory.pngIcon coding.png
araC/pBad-EnvZ* 		inducible EnvZ* October 27
L236 [http://partsregistry.org/Part:BBa_J61002 J61002 ] MP232 S232 Icon regulatory.pngPart icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pFliL(+WT RBS)-EnvZ*-GFP generator 		October 29
L235 [http://partsregistry.org/Part:BBa_J61002 J61002 ] MP233 S233 Icon regulatory.pngPart icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlhB(+WT RBS)-EnvZ*-GFP generator 		October 29




[http://partsregistry.org/Part:BBa_J23100 J23100] [http://partsregistry.org/Part:BBa_J61002 J61002] MP1-2007 MP116 Part icon regulatory.png
Strong constitutive promoter 		July 26
[http://partsregistry.org/Part:BBa_J23107 J23107] [http://partsregistry.org/Part:BBa_J61002 J61002] MP2-2007 MP117 Part icon regulatory.png
Medium constitutive promoter 		July 24, July 26
[http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] MP5-2007 MP120 Part icon rbs.png
Strong RBS (Efficiency = 0,6) 		July 26
[http://partsregistry.org/Part:BBa_B0015 B0015] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] MP3-2007 MP118 Part icon terminator.pngPart icon terminator.png
Double terminator 		July 24, July 26


L174 [http://partsregistry.org/Part:J61002 J61002] S MP202
Icon regulatory.pngIcon coding.png
Strong constitutive promoter-GFP generator 		J23101-E0240 September 26
L157 [http://partsregistry.org/Part:pSB1K3 pSB1AK3] Icon coding.pngIcon rbs.png
tetR-rbs (B0015) 		D125 (BV) - D162 (BI) August 23
L155 [http://partsregistry.org/Part:J61002 J61002] Icon regulatory.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
strongest promoter-gfp generator 		D164(FV) - D163 (FI) August 22
L156 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon regulatory.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pTet-gfp generator 		D161 (FV) - D163 (FI) August 22

Digestion Products

(Legend: Vector, Insert) Ø

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Obs Size Matrix (bp) Size BB (bp) Description Product in use []ng/µL Date
D100.1 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 34 10 July 26
D100.2
D101 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2076 15 10 July 29
D102.1 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2077 14 10 July 26
August 15
D102.2
D103.1 [http://partsregistry.org/Part:BBa_J23101 J23101] [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV 2100 883 27 July 26
D103.2 35
D104.1 [http://partsregistry.org/Part:BBa_J23109 J23109] [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV 2100 883 20 July 26
D104.2
D105.1 [http://partsregistry.org/Part:BBa_R0079 R0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2222 14 5 July 26
D105.2
D106.1 [http://partsregistry.org/Part:BBa_R0040 R0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2119 14 8 July 26
D106.2
D107 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2750 14 August 23 5
12 		July 26
July 23
D108.1 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 707 5 July 29
D108.2
D109 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2056 708 July 26 10 July 26
D110 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2768 14 August 23 20
8
19 		July 26
August 23
August 28
D111.1 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 725 20 July 26
D111.2
D112 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2079 703 8 September 5
D113 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] EcoRI SpeI FI 4402 779 20 July 26
D114 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] XbaI PstI BI 4003 778 20 July 26
D115.1 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 4402 746 - July 29
D115.2
D116.1 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 745 20 July 29
D116.1
D117 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI SpeI FI 3166 746 5 July 29
D118 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB2K3 pSB2K3] XbaI PstI BI 3167 745 20 July 26
D119 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2056 743 10 July 26
D120 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 742 10 July 26
D121 [http://partsregistry.org/Part:BBa_E1010 E1010] [http://partsregistry.org/Part:pSB2K3 pSB2K3] EcoRI SpeI FI 4402 704 10 July 26
D122 [http://partsregistry.org/Part:BBa_E1010 E1010] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 703 10 July 26
D123.1 [http://partsregistry.org/Part:BBa_J23100 J23100] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 26 July 26
D123.2 23
D124.1 [http://partsregistry.org/Part:BBa_J23107 J23107] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 15 July 26
D124.2
D125.1 [http://partsregistry.org/Part:BBa_B0015 B0015] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI XbaI FV 3303 15 26
10 		July 26
August 23
August 28
D125.2 24
3687
D126.1 [http://partsregistry.org/Part:BBa_I0500 I0500] [http://partsregistry.org/Part:pSB2K3 pSB2K3] SpeI PstI BV 5621 14 10 July 26
D126.2
D127.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 37 10 July 26
D127.2
D128.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2079 15 10 July 29
D128.1
D129.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2080 14 10 July 29
D129.2
D130.1 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 939 10 July 26
D130.2
D130.3
D131.1 [http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 900 8 July 26
D131.2
D131.3
D132 pFlgA - EcoRI SpeI FI - 250 5 August 6
D133 pFlgB - EcoRI SpeI FI - 250 5 August 6
D134 pFlhB - EcoRI SpeI FI - 249 5 August 6
D135 pFlhDC - EcoRI SpeI FI - problem August 6
D136 - [http://partsregistry.org/Part:BBa_J61002 J61002] EcoRI SpeI FV 2925 - August 6
August 16
D137 pSB3K3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] EcoRI SpeI FV 2727 - August 6
D138 E0240 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 899 - August 6
D139 flhDC - EcoRI PstI FI - 972 August 6
D140 E040 rbs+ - PstI PstI I - August 12
D141 flhD RBS - - EcoRI SpeI FI - August 12
D142 flhC RBS - - EcoRI SpeI FI - August 12
D143 flhDC + promoter - EcoRI SpeI FI - August 12
D144 pSB3K3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] EcoRI SpeI FI - August 12
D145 pSB1A2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI - August 12
August 15
D146 MP148 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI 2057 1609 rbs TetR - GFP tripart
D110 (BV) - D131 (BI)
Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png 		August 15
D147 MP153 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 2056 799 Strongest RBS (1)- LasR activator (+LVA)
D102 (BV) - D114 (BI)
Part icon rbs.pngIcon coding.png 		August 15
D149 pfliL - EcoRI SpeI FI - August 14
August 16
D150 pfliL - XbaI PstI BI - August 14
August 16
D152 pSB3K3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] XbaI PstI BI - August 14
D153 PCR 138 - gène flhDC - EcoRI SpeI FI - August 15
D154 PCR 140 - gène flhDC - EcoRI SpeI FI - August 15
D155 PCR 139 - pflhDC (URI) - EcoRI SpeI FI - August 15
D158 MP 155.1 - OmpR* [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI - August 15
D159 MP 156.1 - EnvZ* [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI - August 15
D161 MP 104 - pTet [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV - 39 August 16
D162 MP 114 - TetR [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI - 11 August 16
D163 MP 143 - gfp generator [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI - August 23 14
11 		August 16
August 23
D164 MP 101 - pJ23101 [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV - 3 August 16
D165 MP162.2 [http://partsregistry.org/Part:BBa_pSB1AK3 pSB1AK3] XbaI PstI BI 2057 798 rbs-lasR - double terminator
D147 (FI) - D125 (FV)
Part icon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png 		27 August 18
D166 MP 165.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV - 2942 RBS (B0034)+ YFP+ LVA- term B0015
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
59 		August 26
September 3
D179 MP3.4 or MP118 [http://partsregistry.org/Part:BBa_pSB1AK3 pSB1AK3] SpeI PstI BV B0015 (double terminator B0010-B0012)
Part icon terminator.pngPart icon terminator.png 		August 20
D180 MP101.1 [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV promoter J23101
Part icon regulatory.png 		August 20
D181 MP104.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV pTet (TetR repressible promoter)
Part icon regulatory.png 		August 20
D182 MP114.1 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI TetR
Icon coding.png 		August 20
D183 MP119.3 - XbaI PstI BI pBad promoter
Part icon regulatory.png 		August 20
D184 MP143.1 [http://partsregistry.org/Part:BBa_pSB2K3 pSB2K3] EcoRI SpeI FI gfp generator
Part icon reporter.png 		August 18
D185 MP163.1 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV B0032 RBS
Part icon rbs.png 		August 18
D187 MP168.1 [http://partsregistry.org/Part:BBa_pSB1A3 pSB1A3] EcoRI XbaI FV RBS (B0034)+ mRFP LVA+ term B0015
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
43
September 3
D188 PCR 138 - XbaI PstI Insert - 767 OmpR*
Icon coding.png 		August 26
D189 MP 101.2 [http://partsregistry.org/Part:BBa_J61002 J61002] XbaI PstI Vector - 2073 pSB1A2
Icon plasmid.png 		August 26
D191 MP114.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV 2724 tetR
Icon coding.png 		22 August 29
D192 MP144.2 [http://partsregistry.org/Part:BBa_J61002 J61002] EcoRI XbaI FV 2267 FlgA
Part icon regulatory.png 		26 August 29
D193 MP171 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 961 gfp generator(E0240) - pTet
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.pngPart icon regulatory.png 		9,5 August 29
D194 MP172.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 704 RBS(B0032) - tetR
Part icon rbs.pngIcon coding.png 		13 August 29
D195 MP172.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI 703 RBS(B0032) - tetR
Part icon rbs.pngIcon coding.png 		11 August 29
D196 MP104.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV 2119 pTet
Part icon regulatory.png 		45 September 3
D197 MP157.2 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] XbaI PstI BI 898 gfp generator(E0240)
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png 		12 September 3
D198 MP164.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV 2981 RBS (B0034)+ ECFP+ LVA+ term B0015
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png 		36 September 3
D199 MP167.1 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV 2942 RBS (B0034)+ ECFP LVA- term (B0015)
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png 		61 September 3
D200 MP171.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI 960 gfp generator(E0240) - pTet
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.pngPart icon regulatory.png 		43 September 3
D201 MP114.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV 2724 tetR
Icon coding.png 		13 August 29
D202 MP101.1 [http://partsregistry.org/Part:BBa_J61002 J61002] EcoRI nothing 2984 25 September 8
D203 MP101.2 [http://partsregistry.org/Part:BBa_J61002 J61002] BspHI nothing 1870
1008
105 		September 11
D204 MP144 [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI September 22
D205 MP101 [http://partsregistry.org/Part:BBa_J61002 J61002] XbaI PstI September 22
D206 MP179 XbaI PstI September 22
D207 MP182.3 XbaI PstI September
D208 MP175 EcoRI SpeI September
D209 MP144 [http://partsregistry.org/Part:BBa_J61002 J61002] EcoRI SpeI September
D210 MP178 EcoRI SpeI September
Retrieved from "http://2008.igem.org/Team:Paris/Notebook/Freezer"