Team:Chiba/Calendar-Home/7 September 2008

Latest revision as of 22:24, 29 October 2008

Laboratory work

From 2008.igem.org

Team:Input

Team:Communication

Team:Output

(Difference between revisions)

>Home | Notebook

6 September 2008 <|> 8 September 2008

no work

-->(6/9) Digestion Test

Sample DNA 1 : [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](unknown)

Sample DNA 2 : [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](100ng/μL)

Sample No.	1,2
Sample DNA(μL)	12
PstI(μL)	0.5
SpeI(μL)	0.5
Buffer 2(μL)	2
(×10)BSA(μL)	2
dH₂O(μL)	3
TOTAL(μL)	20

--->Gel Check

Sample	left	right
Sample DNA(μL)	5	5
Loading Dye(μL)	1	1
TOTAL(μL)	6	6

Left side:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] Sample No.1

Right side:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] Sample No.2

-->(8/9)

Sample No.

DNA(digested sample)(μL)

Loading Dye(μL)

Cloning Digestion

vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](1)75ng/μl
insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](2) 33.6ng/μl
insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](3) 33.6ng/μl
insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](4) 38.4ng/μl
vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](5) 100ng/μl
insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](6) 33.6ng/μl

Sample No.	1	2	3	4	5	6
DNA(digested sample)(μL)	50	54	54	45	14	54
SpeI(μL)	1	0	0	0	1	0
PstI(μL)	0.5	0.5	0.5	0.5	0.5	0.5
XbaI(μL)	0	0.5	0.5	0.5	0	0.5
BSA(x10)(μL)	0.6	7	7	6	2	7
Buffer2(μL)	6	-	-	-	2	-
Buffer3(μL)	-	7	7	6	-	7
dH₂O(μL)	1.9	1	1	2	0.5	1
TOTAL(μL)	60μl	70μl	70μl	60μl	20μl	70μl

-->left at for 3 hours at 37 degrees.

-->Gel Extraction

-->DNA purification (1~4,6:7μl 5:6μl)

@@ Line 1: / Line 1: @@
 >[[Team:Chiba|Home]] | [[Team:Chiba/Notebook|Notebook]]
-[[Team:Chiba/Calendar-Home/6 September 2008|6 September 2008 <]]|[[Team:Chiba/Calendar-Home/7 September 2008|> 7 September 2008]]
+[[Team:Chiba/Calendar-Home/6 September 2008|6 September 2008 <]]|[[Team:Chiba/Calendar-Home/8 September 2008|> 8 September 2008]]
 ==Laboratory work==
 ===Team:Input===
@@ Line 9: / Line 9: @@
 -->(6/9)
 [[Team:Chiba/protocol/digestion|Digestion Test]]
-::Sample DNA 1 : BBa_R0010(unknown)[http://partsregistry.org/Part:BBa_R0010]
+::Sample DNA 1 : [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](unknown)
-::Sample DNA 2 : BBa_R0010(100ng/μL)[http://partsregistry.org/Part:BBa_R0010]
+::Sample DNA 2 : [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](100ng/μL)
 <table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
 <td>1,2</td>
 </tr>
 <tr>
-<td>Sample DNA</td>
+<td>Sample DNA(μL)</td>
 <td>12</td>
 </tr>
 <tr>
-<td>PstⅠ</td>
+<td>PstI(μL)</td>
 <td>0.5</td>
 </tr>
 <tr>
-<td>SpeⅠ</td>
+<td>SpeI(μL)</td>
 <td>0.5</td>
 </tr>
 <tr>
-<td>Buffer 2</td>
+<td>Buffer 2(μL)</td>
 <td>2</td>
 </tr>
 <tr>
-<td>(×10)BSA</td>
+<td>(×10)BSA(μL)</td>
 <td>2</td>
 </tr>
 <tr>
-<td>dH<sub>2</sub>O</td>
+<td>dH<sub>2</sub>O(μL)</td>
 <td>3</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(μL)</td>
 <td>20</td>
 </tr>
@@ Line 49: / Line 49: @@
 :--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
+{|align="justify"
+|[[Image:Chiba-0907.JPG|300px]]
+|
 :<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
@@ Line 56: / Line 58: @@
 </tr>
 <tr>
-<td>Sample DNA</td>
+<td>Sample DNA(μL)</td>
 <td>5</td><td>5</td>
 </tr>
 <tr>
-<td>Loading Dye</td>
+<td>Loading Dye(μL)</td>
 <td>1</td><td>1</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(μL)</td>
 <td>6</td><td>6</td>
 </tr>
 </table>
 :<br>
-:Left side:BBa_R0010 Sample No.1
+:Left side:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] Sample No.1
-:Right side:BBa_R0010 Sample No.2
+:Right side:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] Sample No.2
+|}
 -->(8/9)
 ===Team:Output===
-*Cloning
+<big>Cloning</big>
 [[Team:Chiba/protocol/digestion|Digestion]]
-*vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)① 75ng/μl
+*vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](1)75ng/μl
-*insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)② 33.6ng/μl
+*insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](2) 33.6ng/μl
-*insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)③ 33.6ng/μl
+*insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](3) 33.6ng/μl
-*insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](RBS+cinR)④ 38.4ng/μl
+*insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](4) 38.4ng/μl
-*vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](pLac)⑤ 100ng/μl
+*vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](5) 100ng/μl
-*insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]⑥ 33.6ng/μl
+*insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](6) 33.6ng/μl
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
 <td width="257">Sample No.</td>
-<td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td><td>⑥</td>
+<td>1</td><td>2</td><td>3</td><td>4</td><td>5</td><td>6</td>
 </tr>
 <tr>
-<td>DNA(digested sample)</td>
+<td>DNA(digested sample)(μL)</td>
 <td>50</td><td>54</td><td>54</td><td>45</td><td>14</td><td>54</td>
 </tr>
 <tr>
-<td>SpeⅠ</td>
+<td>SpeI(μL)</td>
 <td>1</td><td>0</td><td>0</td><td>0</td><td>1</td><td>0</td>
 </tr>
 <tr>
-<td>PstⅠ</td>
+<td>PstI(μL)</td>
 <td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td>
 </tr>
 <tr>
-<td>XbaⅠ</td>
+<td>XbaI(μL)</td>
 <td>0</td><td>0.5</td><td>0.5</td><td>0.5</td><td>0</td><td>0.5</td>
 </tr>
 <tr>
-<td>BSA(x10)</td>
+<td>BSA(x10)(μL)</td>
 <td>0.6</td><td>7</td><td>7</td><td>6</td><td>2</td><td>7</td>
 </tr>
 <tr>
-<td>Buffer2</td>
+<td>Buffer2(μL)</td>
 <td>6</td><td>-</td><td>-</td><td>-</td><td>2</td><td>-</td>
 </tr>
 <tr>
-<td>Buffer3</td>
+<td>Buffer3(μL)</td>
 <td>-</td><td>7</td><td>7</td><td>6</td><td>-</td><td>7<td>
 </tr>
 <tr>
-<td>dH<sub>2</sub>O</td>
+<td>dH<sub>2</sub>O(μL)</td>
 <td>1.9</td><td>1</td><td>1</td><td>2</td><td>0.5</td><td>1</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(μL)</td>
-<td>60</td><td>70</td><td>70</td><td>60</td><td>20</td><td>70</td>
+<td>60μl</td><td>70μl</td><td>70μl</td><td>60μl</td><td>20μl</td><td>70μl</td>
 </tr>
 </table>
--->37℃ 3hour
+-->left at for 3 hours at 37 degrees.
 -->[[Team:Chiba/protocol/ligation/gelex|Gel Extraction]]
@@ Line 135: / Line 137: @@
 <tr>
 <td width="257">Sample No.</td>
-<td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td><td>⑥</td>
+<td>1</td><td>2</td><td>3</td><td>4</td><td>5</td><td>6</td>
 </tr>
 <tr>
-<td>DNA(digested sample)</td>
+<td>DNA(digested sample)(μL)</td>
 <td>6</td><td>10</td><td>10</td><td>10</td><td>4</td><td>10</td>
 </tr>
 <tr>
-<td>Loading Dye</td>
+<td>Loading Dye(μL)</td>
 <td>2</td><td>2</td><td>2</td><td>2</td><td>1</td><td>2</td>
 </tr>
 <tr>
-<td>dH<sub>2</sub>O</td>
+<td>dH<sub>2</sub>O(μL)</td>
 <td>4</td><td>-</td><td>-</td><td>-</td><td>1</td><td>2</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(μL)</td>
 <td>12</td><td>12</td><td>12</td><td>12</td><td>6</td><td>12</td>
 </tr>
@@ Line 158: / Line 160: @@
 </table>
--->[[Team:Chiba/protocol/DNA_Purification/zymocleam|DNA purification]] (①-④,⑥:7μl ⑤:6μl)
+-->[[Team:Chiba/protocol/DNA_Purification/zymocleam|DNA purification]] (1~4,6:7μl 5:6μl)