Team:KULeuven/Project/Output

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Latest revision as of 22:57, 29 October 2008

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Output

We have been searching for an easy accessible output system, that could provide us qualitative and quantitative information in a quick and easy way. The choice for GFP was easily made.

BioBricks

Components

A GFP with a C-terminal LVA tag for rapid degradation of the protein (Andersen et al, 1998) was chosen so that we can follow the output signal quickly in time. The Tet promoter ([http://partsregistry.org/Part:BBa_R0040 BBa_R0040]) behaves as described in the Input section. For fine-tuning reasons, the RBS was chosen [http://partsregistry.org/Part:BBa_B0032 BBa_B0032] with a relative efficiency of 0,3.

Action

This is a very easy system. As the Input results in inactivation of the tetR repressor, there is GFP production, proportional to the input signal.

References

Appl Environ Microbiol. 1998 Jun;64(6):2240-6.
New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria.
Andersen JB, Sternberg C, Poulsen LK, Bjorn SP, Givskov M, Molin S.
PMID: 9603842

@@ Line 1: / Line 1: @@
+{{:Team:KULeuven/Tools/Styling}}
+{{:Team:KULeuven/Tools/Scripting}}
 {{:Team:KULeuven/Tools/Header}}
-<div style="float: right;">[[Image:pictogram_output.png|120px]]</div>
+[[Image:pictogram_output.png|120px|right]]
 ==Output==
+<div style="float:right">[[Image:GFP.png|350px]]</div>
+We have been searching for an easy accessible output system, that could provide us qualitative and quantitative information in a quick and easy way. The choice for GFP was easily made.
-[[Image:Output_BioBrick.jpg|center]]
+===BioBricks===
+[[Image:project_output.jpg|left]]
+<br>
-We have been searching for an easy accesible output system, that could us provide in a quick and easy way qualitative and quantitative information. The choice for GFP was quickly made. We added an LVA C-terminal tag for rapid degradation of the protein, so that we can follow the output signal quickly in time. More information about this type of tagging can be found here ('''link''').
-The OmpF promoter ('''BBa_R0084''') behaves as described in the input section. For fine-tuning reasons, the RBS was chosen '''BBa_B0032''' with a relative efficiency of 0,3.
+===Components===
+A GFP with a C-terminal [https://2008.igem.org/Team:KULeuven/Literature#LVA_tag LVA tag] for rapid degradation of the protein (Andersen et al, 1998) was chosen so that we can follow the output signal quickly in time. The Tet promoter ([http://partsregistry.org/Part:BBa_R0040 '''BBa_R0040''']) behaves as described in the [https://2008.igem.org/Team:KULeuven/Project/Input Input] section. For fine-tuning reasons, the RBS was chosen [http://partsregistry.org/Part:BBa_B0032 '''BBa_B0032'''] with a relative efficiency of 0,3.
+===Action===
+This is a very easy system. As the [https://2008.igem.org/Team:KULeuven/Project/Input Input] results in inactivation of the ''tet''R repressor, there is GFP production, proportional to the input signal.
+==References==
+ Appl Environ Microbiol. 1998 Jun;64(6):2240-6.
+ New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria.
+ Andersen JB, Sternberg C, Poulsen LK, Bjorn SP, Givskov M, Molin S.
+ PMID: 9603842
+{{:Team:KULeuven/Tools/Components}}