Team:Hawaii/Notebook/2008-07-18

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:<strong>Margaret</strong>
:<strong>Margaret</strong>
-
:* Summary of task and what was done. Link to experiment for detailed notes if necessary.
+
:*for details concerning the running conditions, please look [[Team:Hawaii/PCR Amplification of pRL1383a|here]].
-
:* e.g. worked on &lt;blah experiment link&gt;, PCR, ran gel
+
:*Ran a 0.8%gel at 95V. [[Image:PCR_7_18_08.jpg|right|thumb|300px|Please refer to the annotated picture.]]
===Extraction of Biobricks and Transformation in DB3.1===
===Extraction of Biobricks and Transformation in DB3.1===
:<strong>Margaret</strong>
:<strong>Margaret</strong>
 +
:*streaked plates on Amp100 plates
 +
:*for details please look [[Team:Hawaii/Initial E. Coli Transformation|here]].
 +
 +
=== Sequencing===
 +
:<strong>Grace</strong>
 +
 +
:* Redid PCR reactions for GFP fusion, BB-pRL1383a, B0015, J04430, R0010
 +
::* Gel OK this time (95V for 50 min; ladder resolved, loading dye didn't run funny)
 +
::* Incorrect bands still for B0015, J04430, R0010; Krystle will redo the plasmid preps for these
 +
::* Correct bands for GFP fusion and BB-pRL1383a. BB-pRL1383a band faint -- why?
 +
:* Treated PCR products w/ [[Team:Hawaii/Protocols/ExoSAP|ExoSAP]]
 +
:* Determined DNA concentrations using nanodrop spectrometer (measured twice)
 +
{| border="1" align="center"
 +
|+''' DNA concentrations of PCR products'''
 +
!PCR Sample
 +
!Concentration (1st measurement)
 +
!Concentration (2nd measurement)
 +
|-
 +
| align="center"|nir
 +
| align="center"|1015 ng/&mu;l || align="center"|1010 ng/&mu;l
 +
|-
 +
| align="center"|slr2016-1
 +
|align="center"|1295 ng/&mu;l
 +
|align="center"|1107 ng/&mu;l
 +
|-
 +
| align="center"|slr2016-2
 +
| align="center"|1518 ng/&mu;l|| align="center"|1266 ng/&mu;l
 +
|-
 +
| align="center"|pilA
 +
| align="center"|1312 ng/&mu;l|| align="center"|1330 ng/&mu;l
 +
|-
 +
| align="center"| B0024
 +
| align="center"|1546 ng/&mu;l|| align="center"|1474 ng/&mu;l
 +
|-
 +
| align="center"|B0034
 +
| align="center"|1537 ng/&mu;l|| align="center"|1386 ng/&mu;l
 +
|-
 +
| align="center"|C0012
 +
| align="center"|2130 ng/&mu;l|| align="center"|1530 ng/&mu;l
 +
|-
 +
| align="center"|E0040
 +
| align="center"|1502 ng/&mu;l|| align="center"|1236 ng/&mu;l
 +
|-
 +
| align="center"|J33207
 +
| align="center"|1454 ng/&mu;l|| align="center"|1223 ng/&mu;l
 +
|-
 +
| align="center"|BB-pRL1383a
 +
| align="center"|2279 ng/&mu;l|| align="center"|2129 ng/&mu;l
 +
|-
 +
| align="center"|GFP fusion
 +
| align="center"|1660 ng/&mu;l|| align="center"|1714 ng/&mu;l
 +
|}
 +
:* Sent 22 samples to CORE Hawaii for sequencing
 +
 +
===Media Making===
 +
<strong>Margaret, Krystle (thanks for the help!)</strong>
 +
:* Made one sleeve of Amp100 plates.
 +
 +
===Plasmid Prep of GFP fusion Brick, R0010, J3340===
 +
:<strong>Krystle</strong>
 +
 +
:*did a large scale (75 ml) plasmid prep for the GFP fusion brick
 +
:*Performed 3 mini preps each of the BioBrick parts
== Drylab Work ==
== Drylab Work ==
-
===Name of Task===
 
-
:<strong> name of person/people who performed the task</strong>
 
-
:* Summary of task and what was done. Link to experiment for detailed notes if necessary.
+
===[[Team:Hawaii/Project|Project Description (Abstract]])===
-
:* e.g. read through papers, worked on proposal, etc.
+
:<strong> Grace, Krystle, Margaret</strong>
 +
:* Wrote description of project (abstract)
= Discussion =
= Discussion =

Latest revision as of 04:08, 22 July 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

PCR of pRL1383a Parts

Margaret
  • for details concerning the running conditions, please look here.
  • Ran a 0.8%gel at 95V.
    Please refer to the annotated picture.

Extraction of Biobricks and Transformation in DB3.1

Margaret
  • streaked plates on Amp100 plates
  • for details please look here.

Sequencing

Grace
  • Redid PCR reactions for GFP fusion, BB-pRL1383a, B0015, J04430, R0010
  • Gel OK this time (95V for 50 min; ladder resolved, loading dye didn't run funny)
  • Incorrect bands still for B0015, J04430, R0010; Krystle will redo the plasmid preps for these
  • Correct bands for GFP fusion and BB-pRL1383a. BB-pRL1383a band faint -- why?
  • Treated PCR products w/ ExoSAP
  • Determined DNA concentrations using nanodrop spectrometer (measured twice)
DNA concentrations of PCR products
PCR Sample Concentration (1st measurement) Concentration (2nd measurement)
nir 1015 ng/μl 1010 ng/μl
slr2016-1 1295 ng/μl 1107 ng/μl
slr2016-2 1518 ng/μl1266 ng/μl
pilA 1312 ng/μl1330 ng/μl
B0024 1546 ng/μl1474 ng/μl
B0034 1537 ng/μl1386 ng/μl
C0012 2130 ng/μl1530 ng/μl
E0040 1502 ng/μl1236 ng/μl
J33207 1454 ng/μl1223 ng/μl
BB-pRL1383a 2279 ng/μl2129 ng/μl
GFP fusion 1660 ng/μl1714 ng/μl
  • Sent 22 samples to CORE Hawaii for sequencing

Media Making

Margaret, Krystle (thanks for the help!)

  • Made one sleeve of Amp100 plates.

Plasmid Prep of GFP fusion Brick, R0010, J3340

Krystle
  • did a large scale (75 ml) plasmid prep for the GFP fusion brick
  • Performed 3 mini preps each of the BioBrick parts

Drylab Work

Project Description (Abstract)

Grace, Krystle, Margaret
  • Wrote description of project (abstract)

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]


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