Latest revision as of 04:08, 22 July 2008

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Things we did today

Wetlab work

PCR of pRL1383a Parts

Margaret

for details concerning the running conditions, please look here.
Ran a 0.8%gel at 95V.

Please refer to the annotated picture.

Extraction of Biobricks and Transformation in DB3.1

Margaret

streaked plates on Amp100 plates

for details please look here.

Sequencing

Grace

Redid PCR reactions for GFP fusion, BB-pRL1383a, B0015, J04430, R0010

Gel OK this time (95V for 50 min; ladder resolved, loading dye didn't run funny)
Incorrect bands still for B0015, J04430, R0010; Krystle will redo the plasmid preps for these
Correct bands for GFP fusion and BB-pRL1383a. BB-pRL1383a band faint -- why?

Treated PCR products w/ ExoSAP
Determined DNA concentrations using nanodrop spectrometer (measured twice)

**DNA concentrations of PCR products**
PCR Sample	Concentration (1st measurement)	Concentration (2nd measurement)
nir	1015 ng/μl	1010 ng/μl
slr2016-1	1295 ng/μl	1107 ng/μl
slr2016-2	1518 ng/μl	1266 ng/μl
pilA	1312 ng/μl	1330 ng/μl
B0024	1546 ng/μl	1474 ng/μl
B0034	1537 ng/μl	1386 ng/μl
C0012	2130 ng/μl	1530 ng/μl
E0040	1502 ng/μl	1236 ng/μl
J33207	1454 ng/μl	1223 ng/μl
BB-pRL1383a	2279 ng/μl	2129 ng/μl
GFP fusion	1660 ng/μl	1714 ng/μl

Sent 22 samples to CORE Hawaii for sequencing

Media Making

Margaret, Krystle (thanks for the help!)

Made one sleeve of Amp100 plates.

Plasmid Prep of GFP fusion Brick, R0010, J3340

Krystle

did a large scale (75 ml) plasmid prep for the GFP fusion brick
Performed 3 mini preps each of the BioBrick parts

Drylab Work

Project Description (Abstract)

Grace, Krystle, Margaret

Wrote description of project (abstract)

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 6: / Line 6: @@
 :<strong>Margaret</strong>
-:*for details please look [[Team:Hawaii/PCR Amplification of pRL1383a|here]].
+:*for details concerning the running conditions, please look [[Team:Hawaii/PCR Amplification of pRL1383a|here]].
+:*Ran a 0.8%gel at 95V. [[Image:PCR_7_18_08.jpg|right|thumb|300px|Please refer to the annotated picture.]]
 ===Extraction of Biobricks and Transformation in DB3.1===
 :<strong>Margaret</strong>
+:*streaked plates on Amp100 plates
 :*for details please look [[Team:Hawaii/Initial E. Coli Transformation|here]].
@@ Line 21: / Line 23: @@
 ::* Correct bands for GFP fusion and BB-pRL1383a. BB-pRL1383a band faint -- why?
 :* Treated PCR products w/ [[Team:Hawaii/Protocols/ExoSAP|ExoSAP]]
-:* Determined DNA concentrations using nanodrop spectrometer
+:* Determined DNA concentrations using nanodrop spectrometer (measured twice)
-:* Sent 11 samples to CORE Hawaii for sequencing
+{| border="1" align="center"
+|+''' DNA concentrations of PCR products'''
+!PCR Sample
+!Concentration (1st measurement)
+!Concentration (2nd measurement)
+|-
+| align="center"|nir
+| align="center"|1015 ng/&mu;l || align="center"|1010 ng/&mu;l
+|-
+| align="center"|slr2016-1
+|align="center"|1295 ng/&mu;l
+|align="center"|1107 ng/&mu;l
+|-
+| align="center"|slr2016-2
+| align="center"|1518 ng/&mu;l|| align="center"|1266 ng/&mu;l
+|-
+| align="center"|pilA
+| align="center"|1312 ng/&mu;l|| align="center"|1330 ng/&mu;l
+|-
+| align="center"| B0024
+| align="center"|1546 ng/&mu;l|| align="center"|1474 ng/&mu;l
+|-
+| align="center"|B0034
+| align="center"|1537 ng/&mu;l|| align="center"|1386 ng/&mu;l
+|-
+| align="center"|C0012
+| align="center"|2130 ng/&mu;l|| align="center"|1530 ng/&mu;l
+|-
+| align="center"|E0040
+| align="center"|1502 ng/&mu;l|| align="center"|1236 ng/&mu;l
+|-
+| align="center"|J33207
+| align="center"|1454 ng/&mu;l|| align="center"|1223 ng/&mu;l
+|-
+| align="center"|BB-pRL1383a
+| align="center"|2279 ng/&mu;l|| align="center"|2129 ng/&mu;l
+|-
+| align="center"|GFP fusion
+| align="center"|1660 ng/&mu;l|| align="center"|1714 ng/&mu;l
+|}
+:* Sent 22 samples to CORE Hawaii for sequencing
+===Media Making===
+<strong>Margaret, Krystle (thanks for the help!)</strong>
+:* Made one sleeve of Amp100 plates.
+===Plasmid Prep of GFP fusion Brick, R0010, J3340===
+:<strong>Krystle</strong>
+:*did a large scale (75 ml) plasmid prep for the GFP fusion brick
+:*Performed 3 mini preps each of the BioBrick parts
 == Drylab Work ==
-===Name of Task===
-:<strong> name of person/people who performed the task</strong>
-:* Summary of task and what was done. Link to experiment for detailed notes if necessary.
+===[[Team:Hawaii/Project|Project Description (Abstract]])===
-:* e.g. read through papers, worked on proposal, etc.
+:<strong> Grace, Krystle, Margaret</strong>
+:* Wrote description of project (abstract)
 = Discussion =

Team:Hawaii/Notebook/2008-07-18

From 2008.igem.org