Team:KULeuven/Project/Output

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Latest revision as of 22:57, 29 October 2008

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Output

We have been searching for an easy accessible output system, that could provide us qualitative and quantitative information in a quick and easy way. The choice for GFP was easily made.

BioBricks

Components

A GFP with a C-terminal LVA tag for rapid degradation of the protein (Andersen et al, 1998) was chosen so that we can follow the output signal quickly in time. The Tet promoter ([http://partsregistry.org/Part:BBa_R0040 BBa_R0040]) behaves as described in the Input section. For fine-tuning reasons, the RBS was chosen [http://partsregistry.org/Part:BBa_B0032 BBa_B0032] with a relative efficiency of 0,3.

Action

This is a very easy system. As the Input results in inactivation of the tetR repressor, there is GFP production, proportional to the input signal.

References

Appl Environ Microbiol. 1998 Jun;64(6):2240-6.
New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria.
Andersen JB, Sternberg C, Poulsen LK, Bjorn SP, Givskov M, Molin S.
PMID: 9603842

@@ Line 1: / Line 1: @@
+{{:Team:KULeuven/Tools/Styling}}
+{{:Team:KULeuven/Tools/Scripting}}
 {{:Team:KULeuven/Tools/Header}}
-<div style="float: right;">[[Image:pictogram_output.png|120px]]</div>
+[[Image:pictogram_output.png|120px|right]]
 ==Output==
-<div style="float:left">[[Image:GFP.png|400px]]</div>
+<div style="float:right">[[Image:GFP.png|350px]]</div>
 We have been searching for an easy accessible output system, that could provide us qualitative and quantitative information in a quick and easy way. The choice for GFP was easily made.
 ===BioBricks===
-[[Image:project_output.jpg|center]]
+[[Image:project_output.jpg|left]]
+<br>
 ===Components===
-We added C-terminal a [https://2008.igem.org/Team:KULeuven/Literature#LVA_tag LVA tag] for rapid degradation of the protein, so that we can follow the output signal quickly in time. The OmpF promoter ([http://partsregistry.org/Part:BBa_R0084 '''BBa_R0084''']) behaves as described in the [https://2008.igem.org/Team:KULeuven/Project/Input input] section. For fine-tuning reasons, the RBS was chosen [http://partsregistry.org/Part:BBa_B0032 '''BBa_B0032'''] with a relative efficiency of 0,3.
+A GFP with a C-terminal [https://2008.igem.org/Team:KULeuven/Literature#LVA_tag LVA tag] for rapid degradation of the protein (Andersen et al, 1998) was chosen so that we can follow the output signal quickly in time. The Tet promoter ([http://partsregistry.org/Part:BBa_R0040 '''BBa_R0040''']) behaves as described in the [https://2008.igem.org/Team:KULeuven/Project/Input Input] section. For fine-tuning reasons, the RBS was chosen [http://partsregistry.org/Part:BBa_B0032 '''BBa_B0032'''] with a relative efficiency of 0,3.
 ===Action===
-This is a very easy system. As the [https://2008.igem.org/Team:KULeuven/Project/Input input] results in a low concentration of phosphorylated OmpR, there is GFP production, proportional to the input signal.
+This is a very easy system. As the [https://2008.igem.org/Team:KULeuven/Project/Input Input] results in inactivation of the ''tet''R repressor, there is GFP production, proportional to the input signal.
+==References==
+ Appl Environ Microbiol. 1998 Jun;64(6):2240-6.
+ New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria.
+ Andersen JB, Sternberg C, Poulsen LK, Bjorn SP, Givskov M, Molin S.
+ PMID: 9603842
 {{:Team:KULeuven/Tools/Components}}