Team:KULeuven/30 August 2008

From 2008.igem.org

(Difference between revisions)

Latest revision as of 15:30, 12 October 2008

dock/undock dropdown

<< return to notebook			return to homepage >>
< previous friday	← yesterday	tomorrow →	next monday >

Lab Work

Wet Lab

A glycerol stock was made of the new parts and of our own parts ([http://partsregistry.org/Part:BBa_K145151 K145151], [http://partsregistry.org/Part:BBa_K145001 K145001], [http://partsregistry.org/Part:BBa_K145015 K145015]). We forgot one, so a liquid culture was made of part [http://partsregistry.org/Part:BBa_R0053 R0053].

We miniprepped the new parts and the two ligations that succeeded ([http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015] and [http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015] that were plated on Ap instead of Km). They were then cut with XbaI.

The digests with XbaI were started: [http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_B0033 B0033], [http://partsregistry.org/Part:BBa_B0034 B0034], [http://partsregistry.org/Part:BBa_J23066 J23066], [http://partsregistry.org/Part:BBa_P0353 P0353], [http://partsregistry.org/Part:BBa_P0152 P0152], [http://partsregistry.org/Part:BBa_E0240 E0240].

The ligations we made yesterday were electroporated today (still in electrocompetent Top10 cells): [http://partsregistry.org/Part:BBa_R0011 R0011]+[http://partsregistry.org/Part:BBa_B0032 B0032], [http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145001 K145001]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015] and ([http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022])+([http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]).

Dry Lab

General

Created the oligos for the B1002 and B1006 terminators [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=terminator link]

Modeling

Final updated model uploaded to the wiki (only MatLab)

Remarks

I don't think the dam methylation is the problem with our ligations. This is the prefix: gaattcgcggccgcttctagag. The recognition site of XbaI is in bold. There is no overlap for dam methylation (gatc).

@@ Line 1: / Line 1: @@
+{{:Team:KULeuven/Tools/Styling}}
 {{:Team:KULeuven/Tools/Header}}
+{{:Team:KULeuven/Tools/New_Day/Date_Retriever}}
 == Lab Work ==
@@ Line 5: / Line 8: @@
 === Wet Lab ===
-* A glycerol stock was made of the new parts and of our own parts (K145151, K145001 and K145015).
+A glycerol stock was made of the new parts and of our own parts ([http://partsregistry.org/Part:BBa_K145151 K145151], [http://partsregistry.org/Part:BBa_K145001 K145001], [http://partsregistry.org/Part:BBa_K145015 K145015]). We forgot one, so a liquid culture was made of part [http://partsregistry.org/Part:BBa_R0053 R0053].
-* We MiniPrepped the new parts and the two ligations that succeeded (C0040+B0015 and C0060+B0015).
-* The digestions with ''Xba''I were started.
+We miniprepped the new parts and the two ligations that succeeded ([http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015] and [http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015] that were plated on Ap instead of Km). They were then cut with ''Xba''I.
-* The ligations we made yesterday were electroporated today (still in electrocompetent Top10 cells).
-* A liquid culture was made of part R0053.
+The digests with ''Xba''I were started: [http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_B0033 B0033], [http://partsregistry.org/Part:BBa_B0034 B0034], [http://partsregistry.org/Part:BBa_J23066 J23066], [http://partsregistry.org/Part:BBa_P0353 P0353], [http://partsregistry.org/Part:BBa_P0152 P0152], [http://partsregistry.org/Part:BBa_E0240 E0240].
+The ligations we made yesterday were electroporated today (still in electrocompetent Top10 cells): [http://partsregistry.org/Part:BBa_R0011 R0011]+[http://partsregistry.org/Part:BBa_B0032 B0032], [http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145001 K145001]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015] and ([http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022])+([http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]).
 === Dry Lab ===
+==== General ====
 Created the oligos for the B1002 and B1006 terminators [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=terminator link]
 ==== Modeling ====
 Final updated model uploaded to the wiki (only MatLab)
-==== Wiki ====
 == Remarks ==
 I don't think the dam methylation is the problem with our ligations. This is the prefix:
 gaattcgcggccgct'''tctaga'''g. The recognition site of ''Xba''I is in bold. There is no overlap for dam methylation (gatc).
-{{:Team:KULeuven/Tools/New_Day/Date_Retriever}}