Team:Chiba/Calendar-Home/7 September 2008

From 2008.igem.org

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</tr>
</tr>
<tr>
<tr>
-
<td>PstⅠ(μL)</td>
+
<td>PstI(μL)</td>
<td>0.5</td>
<td>0.5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>SpeⅠ(μL)</td>
+
<td>SpeI(μL)</td>
<td>0.5</td>
<td>0.5</td>
</tr>
</tr>
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:--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
:--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
-
 
+
{|align="justify"
 +
|[[Image:Chiba-0907.JPG|300px]]
 +
|
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
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:Left side:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] Sample No.1
:Left side:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] Sample No.1
:Right side:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] Sample No.2
:Right side:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] Sample No.2
 +
|}
-->(8/9)
-->(8/9)
-
 
===Team:Output===
===Team:Output===
<big>Cloning</big>
<big>Cloning</big>
[[Team:Chiba/protocol/digestion|Digestion]]
[[Team:Chiba/protocol/digestion|Digestion]]
-
*vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]① 75ng/μl
+
*vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](1)75ng/μl
-
*insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158]② 33.6ng/μl  
+
*insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](2) 33.6ng/μl  
-
*insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156]③ 33.6ng/μl
+
*insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](3) 33.6ng/μl
-
*insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160]④ 38.4ng/μl
+
*insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](4) 38.4ng/μl
-
*vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]⑤ 100ng/μl
+
*vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](5) 100ng/μl
-
*insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]⑥ 33.6ng/μl
+
*insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](6) 33.6ng/μl
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
<td width="257">Sample No.</td>
<td width="257">Sample No.</td>
-
<td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td><td>⑥</td>
+
<td>1</td><td>2</td><td>3</td><td>4</td><td>5</td><td>6</td>
</tr>
</tr>
<tr>
<tr>
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</tr>
</tr>
<tr>
<tr>
-
<td>SpeⅠ(μL)</td>
+
<td>SpeI(μL)</td>
<td>1</td><td>0</td><td>0</td><td>0</td><td>1</td><td>0</td>
<td>1</td><td>0</td><td>0</td><td>0</td><td>1</td><td>0</td>
</tr>
</tr>
<tr>
<tr>
-
<td>PstⅠ(μL)</td>
+
<td>PstI(μL)</td>
<td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td>
<td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>XbaⅠ(μL)</td>
+
<td>XbaI(μL)</td>
<td>0</td><td>0.5</td><td>0.5</td><td>0.5</td><td>0</td><td>0.5</td>
<td>0</td><td>0.5</td><td>0.5</td><td>0.5</td><td>0</td><td>0.5</td>
</tr>
</tr>
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<tr>
<tr>
<td width="257">Sample No.</td>
<td width="257">Sample No.</td>
-
<td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td><td>⑥</td>
+
<td>1</td><td>2</td><td>3</td><td>4</td><td>5</td><td>6</td>
</tr>
</tr>
<tr>
<tr>
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</table>
</table>
-
-->[[Team:Chiba/protocol/DNA_Purification/zymocleam|DNA purification]] (①-④,⑥:7μl ⑤:6μl)
+
-->[[Team:Chiba/protocol/DNA_Purification/zymocleam|DNA purification]] (1~4,6:7μl 5:6μl)

Latest revision as of 22:24, 29 October 2008

>Home | Notebook

6 September 2008 <|> 8 September 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

-->(6/9) Digestion Test

Sample DNA 1 : [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](unknown)
Sample DNA 2 : [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](100ng/μL)
Sample No. 1,2
Sample DNA(μL) 12
PstI(μL) 0.5
SpeI(μL) 0.5
Buffer 2(μL) 2
(×10)BSA(μL) 2
dH2O(μL) 3
TOTAL(μL) 20


--->Gel Check
Chiba-0907.JPG
Sample leftright
Sample DNA(μL) 55
Loading Dye(μL) 11
TOTAL(μL) 66

Left side:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] Sample No.1
Right side:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] Sample No.2

-->(8/9)

Team:Output

Cloning Digestion

  • vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](1)75ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](2) 33.6ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](3) 33.6ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](4) 38.4ng/μl
  • vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](5) 100ng/μl
  • insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](6) 33.6ng/μl
Sample No. 123456
DNA(digested sample)(μL) 505454451454
SpeI(μL) 100010
PstI(μL) 0.50.50.50.50.50.5
XbaI(μL) 00.50.50.500.5
BSA(x10)(μL) 0.677627
Buffer2(μL) 6---2-
Buffer3(μL) -776-7
dH2O(μL) 1.91120.51
TOTAL(μL) 60μl70μl70μl60μl20μl70μl

-->left at for 3 hours at 37 degrees.

-->Gel Extraction

Sample No. 123456
DNA(digested sample)(μL) 6101010410
Loading Dye(μL) 222212
dH2O(μL) 4---12
TOTAL(μL) 12121212612
well 1077657

-->DNA purification (1~4,6:7μl 5:6μl)

Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/7_September_2008"