Team:Chiba/Calendar-Home/6 September 2008

From 2008.igem.org

(Difference between revisions)
(→Team:Communication)
 
(5 intermediate revisions not shown)
Line 14: Line 14:
===Team:Communication===
===Team:Communication===
'''[[Team:Chiba/protocol/PCR|Colony-PCR]]'''
'''[[Team:Chiba/protocol/PCR|Colony-PCR]]'''
-
::PCR of BBa_I9030 and two control,BBa_F2620[http://partsregistry.org/Part:BBa_F2620](2007)).
+
::PCR of [http://partsregistry.org/Part:BBa_I9030 BBa_I9030] and two control,[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)).
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
Line 52: Line 52:
-
:95℃,5min -> ( 95℃,1min -> 54℃,1min -> 72℃,1min )･･･25cycles -> 72℃,10min -> 6℃ store
+
:95&deg;C,5min -> ( 95&deg;C,1min -> 54&deg;C,1min -> 72&deg;C,1min )･･･25cycles -> 72&deg;C,10min -> 6&deg;C store
:--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
:--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
-
 
+
{|align="justify"
 +
|[[Image:Chiba-0906.JPG|300px]]
 +
|
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
Line 72: Line 74:
<tr>
<tr>
<td>TOTAL</td>
<td>TOTAL</td>
-
<td>6</td></tr>
+
<td>6μl</td></tr>
</table>
</table>
:From left;<br>
:From left;<br>
-
:1   I9030 without LVA  -> OK
+
:1 [http://partsregistry.org/Part:BBa_I9030 BBa_I9030] without LVA  -> OK
-
:2   I9030 Positive Control  -> OK
+
:2 [http://partsregistry.org/Part:BBa_I9030 BBa_I9030] Positive Control  -> OK
-
:3   F2620 Positive control  -> OK
+
:3 [http://partsregistry.org/Part:BBa_F2620 BBa_F2620] Positive control  -> OK
 +
|}
'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
-
Sample DNA : R0010
+
{|align="justify"
 +
|[[Image:Chiba-0906-2.JPG|300px]]
 +
|
 +
Sample DNA : [http://partsregistry.org/Part:BBa_R0010 BBa_R0010]
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
Line 96: Line 102:
<tr>
<tr>
<td>TOTAL</td>
<td>TOTAL</td>
-
<td>6</td>
+
<td>6μl</td>
</tr>
</tr>
</table>
</table>
-
 
+
:R0010 --> 100μL
 +
|}
Line 105: Line 112:
'''[[Team:Chiba/protocol/digestion|Digestion Test]]'''
'''[[Team:Chiba/protocol/digestion|Digestion Test]]'''
:Sample DNA:
:Sample DNA:
-
1:R0010,2:S03154,3:J04500,4:C0161,5:C0261
+
1:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010],2:[http://partsregistry.org/Part:BBa_S03154 BBa_S03154],3:[http://partsregistry.org/Part:BBa_J04500 BBa_J04500],4:[http://partsregistry.org/Part:BBa_C0161 BBa_C0161],5:[http://partsregistry.org/Part:BBa_C0261 BBa_C0261]
 +
 
<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
Line 115: Line 123:
</tr>
</tr>
<tr>
<tr>
-
<td>PstⅠ</td>
+
<td>PstI</td>
<td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td>
<td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>SpeⅠ</td>
+
<td>SpeI</td>
<td>0.5</td><td>-</td><td>1</td><td>-</td><td>-</td>
<td>0.5</td><td>-</td><td>1</td><td>-</td><td>-</td>
</tr>
</tr>
<tr>
<tr>
-
<td>XbaⅠ</td>
+
<td>XbaI</td>
<td>-</td><td>0.5</td><td>-</td><td>0.5</td><td>0.5</td>
<td>-</td><td>0.5</td><td>-</td><td>0.5</td><td>0.5</td>
</tr>
</tr>
Line 148: Line 156:
<tr>
<tr>
<td>TOTAL</td>
<td>TOTAL</td>
-
<td>20</td><td>60</td><td>30</td><td>60</td><td>60</td>
+
<td>20μl</td><td>60μl</td><td>30μl</td><td>60μl</td><td>60μl</td>
</tr>
</tr>
</table>
</table>
-
 
===Team:Output===
===Team:Output===
no work
no work

Latest revision as of 22:19, 29 October 2008

>Home | Notebook

5 September 2008 <|> 7 September 2008

Contents

Meeting with UmeG

the ppt (in Japanese) for our weekly meeting

  1. File:Chiba-0809060855.ppt


Laboratory work

Team:Input

no work

Team:Communication

Colony-PCR

PCR of [http://partsregistry.org/Part:BBa_I9030 BBa_I9030] and two control,[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)).
DNA Template 1
dNTP mix 5
Foward Primer 5
Reverse Primer 5
DNA polymerase 1
Thermopol Buffer 5
dH2O 33
TOTAL 50μL


95°C,5min -> ( 95°C,1min -> 54°C,1min -> 72°C,1min )･･･25cycles -> 72°C,10min -> 6°C store


--->Gel Check
Chiba-0906.JPG
Sample DNA 1
Loading Dye 1
dH2O 4
TOTAL 6μl
From left;
1 [http://partsregistry.org/Part:BBa_I9030 BBa_I9030] without LVA -> OK
2 [http://partsregistry.org/Part:BBa_I9030 BBa_I9030] Positive Control -> OK
3 [http://partsregistry.org/Part:BBa_F2620 BBa_F2620] Positive control -> OK

Gel Check

Chiba-0906-2.JPG

Sample DNA : [http://partsregistry.org/Part:BBa_R0010 BBa_R0010]

Sample DNA 1
Loading Dye 1
dH2O 4
TOTAL 6μl
R0010 --> 100μL


Digestion Test

Sample DNA:

1:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010],2:[http://partsregistry.org/Part:BBa_S03154 BBa_S03154],3:[http://partsregistry.org/Part:BBa_J04500 BBa_J04500],4:[http://partsregistry.org/Part:BBa_C0161 BBa_C0161],5:[http://partsregistry.org/Part:BBa_C0261 BBa_C0261]


Sample No. 12345
Sample DNA 11111
PstI 0.50.50.50.50.5
SpeI 0.5-1--
XbaI -0.5-0.50.5
Buffer 2 2-3--
Buffer 3 -6-66
(10×)BSA 20.63--
(100×)BSA ---0.60.6
dH2O 36.471.41.4
TOTAL 20μl60μl30μl60μl60μl

Team:Output

no work

Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/6_September_2008"