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Brown: Team Resistance/26 October 2008
From 2008.igem.org
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*Mercury inducible promoter | *Mercury inducible promoter | ||
*Pu inducible promoter (xylene induced) | *Pu inducible promoter (xylene induced) | ||
| - | * | + | *T7 promoter, with activity modified by change in temperature |
Also re-transformed ligations of the pVJ4 cassette and the S105 cassette (both transformations unsuccessful in the past) | Also re-transformed ligations of the pVJ4 cassette and the S105 cassette (both transformations unsuccessful in the past) | ||
*Will run a gel of the S105 gene restriction digest with EcoRI and SpeI for verification of part. The gel showed a faint band at the length we hoped for but there was no other band verifying the vector. We should have seen two bands but we only saw one. | *Will run a gel of the S105 gene restriction digest with EcoRI and SpeI for verification of part. The gel showed a faint band at the length we hoped for but there was no other band verifying the vector. We should have seen two bands but we only saw one. | ||
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====Conductivity Tests==== | ====Conductivity Tests==== | ||
Latest revision as of 21:52, 27 October 2008
26 October 2008Biobricking work
Began transforming promoters from the registry in order to ligate them to our cassette and create composite parts:
Also re-transformed ligations of the pVJ4 cassette and the S105 cassette (both transformations unsuccessful in the past)
Conductivity Tests
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