Team:Paris/Notebook/Freezer

From 2008.igem.org

(Difference between revisions)
(→Plasmids: MiniPrep Products)
(→Plasmids: MiniPrep Products)
Line 356: Line 356:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |MP124
| style="background: #D4E2EF;" |MP124
-
|align="center"|L102
+
|align="center"|L102 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
 +
 
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]
|align="center"|Strong rbs - YFP<br>D129 (BV) -  D118 (BI)
|align="center"|Strong rbs - YFP<br>D129 (BV) -  D118 (BI)
Line 363: Line 364:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |MP125
| style="background: #D4E2EF;" |MP125
-
|align="center"|L103
+
|align="center"|L103 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]
|align="center"|Strong rbs - mRFP<br>D129 (BV) -  D122 (BI)
|align="center"|Strong rbs - mRFP<br>D129 (BV) -  D122 (BI)
Line 370: Line 371:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |MP126
| style="background: #D4E2EF;" |MP126
-
|align="center"|L104
+
|align="center"|L104 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]
|align="center"|Strong rbs - lasR activator<br>D129 (BV) -  D114 (BI)
|align="center"|Strong rbs - lasR activator<br>D129 (BV) -  D114 (BI)
Line 377: Line 378:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |MP127
| style="background: #D4E2EF;" |MP127
-
|align="center"|L105
+
|align="center"|L105 in [http://partsregistry.org/Part:J61002 J61002]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
|align="center"|Strong promoter - ECFP<br>D123 (BV) - D130 (BI)
|align="center"|Strong promoter - ECFP<br>D123 (BV) - D130 (BI)
Line 384: Line 385:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |MP128
| style="background: #D4E2EF;" |MP128
-
|align="center"|L106
+
|align="center"|L106 in [http://partsregistry.org/Part:J61002 J61002]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
|align="center"|Strong promoter - gfp Tripart<br>D123 (BV) - D131 (BI)
|align="center"|Strong promoter - gfp Tripart<br>D123 (BV) - D131 (BI)
Line 391: Line 392:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |MP129
| style="background: #D4E2EF;" |MP129
-
|align="center"|L107
+
|align="center"|L107 in [http://partsregistry.org/Part:J61002 J61002]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
|align="center"|Strong promoter - ECFP<br>D103 (BV) - D130 (BI)
|align="center"|Strong promoter - ECFP<br>D103 (BV) - D130 (BI)
Line 398: Line 399:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |MP130
| style="background: #D4E2EF;" |MP130
-
|align="center"|L108
+
|align="center"|L108 in [http://partsregistry.org/Part:J61002 J61002]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
|align="center"|Strong promoter -  gfp Tripart<br>D103 (BV) - D131 (BI)
|align="center"|Strong promoter -  gfp Tripart<br>D103 (BV) - D131 (BI)
Line 405: Line 406:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |MP131
| style="background: #D4E2EF;" |MP131
-
|align="center"|L110
+
|align="center"|L110 in [http://partsregistry.org/Part:J61002 J61002]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
|align="center"|Medium promoter -  gfp Tripart<br>D124 (BV) - D131 (BI)
|align="center"|Medium promoter -  gfp Tripart<br>D124 (BV) - D131 (BI)
Line 412: Line 413:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |MP132
| style="background: #D4E2EF;" |MP132
-
|align="center"|L111
+
|align="center"|L111 in [http://partsregistry.org/Part:J61002 J61002]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
|align="center"|Weak promoter - ECFP<br>D104 (BV) - D130 (BI)
|align="center"|Weak promoter - ECFP<br>D104 (BV) - D130 (BI)
Line 419: Line 420:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |MP133
| style="background: #D4E2EF;" |MP133
-
|align="center"|L112
+
|align="center"|L112 in [http://partsregistry.org/Part:J61002 J61002]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
|align="center"|Weak promoter - GFP tripart<br>D104 (BV) - D131 (BI)
|align="center"|Weak promoter - GFP tripart<br>D104 (BV) - D131 (BI)
Line 426: Line 427:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |MP134
| style="background: #D4E2EF;" |MP134
-
|align="center"|L115
+
|align="center"|L115 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
|align="center"|pLas - ECFP<br>D105 (BV) - D130 (BI)
|align="center"|pLas - ECFP<br>D105 (BV) - D130 (BI)
Line 433: Line 434:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |MP135
| style="background: #D4E2EF;" |MP135
-
|align="center"|L116
+
|align="center"|L116 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
|align="center"|pLas - gfp Tripart<br>D105 (BV) - D131 (BI)
|align="center"|pLas - gfp Tripart<br>D105 (BV) - D131 (BI)
Line 440: Line 441:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |MP136
| style="background: #D4E2EF;" |MP136
-
|align="center"|L117
+
|align="center"|L117 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
|align="center"|[[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
|align="center"|yfp - Double Terminator<br>D125 (FV) - D117 (FI)
|align="center"|yfp - Double Terminator<br>D125 (FV) - D117 (FI)
Line 447: Line 448:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |MP137
| style="background: #D4E2EF;" |MP137
-
|align="center"|L118
+
|align="center"|L118 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
|align="center"|[[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
|align="center"|rfp - Double Terminator<br>D125 (FV) - D121 (FI)
|align="center"|rfp - Double Terminator<br>D125 (FV) - D121 (FI)
Line 454: Line 455:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |MP138
| style="background: #D4E2EF;" |MP138
-
|align="center"|L119
+
|align="center"|L119 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
|align="center"|[[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
|align="center"|lasR activator + LVA - Double Terminator<br>D125 (FV) - D113 (FI)
|align="center"|lasR activator + LVA - Double Terminator<br>D125 (FV) - D113 (FI)
Line 461: Line 462:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |MP139
| style="background: #D4E2EF;" |MP139
-
|align="center"|L121
+
|align="center"|L121 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
|align="center"|pTet - gfp tripart<br>D106 (BV) - D131 (BI)
|align="center"|pTet - gfp tripart<br>D106 (BV) - D131 (BI)
Line 468: Line 469:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |MP140
| style="background: #D4E2EF;" |MP140
-
|align="center"|L124
+
|align="center"|L124 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]
|align="center"|Strongest RBS - rfp<br>D102 (BV) - D122 (BI)
|align="center"|Strongest RBS - rfp<br>D102 (BV) - D122 (BI)
Line 475: Line 476:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |MP141
| style="background: #D4E2EF;" |MP141
-
|align="center"|L125
+
|align="center"|L125 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]
|align="center"|Strongest RBS - yfp<br>D102 (BV) - D118 (BI)
|align="center"|Strongest RBS - yfp<br>D102 (BV) - D118 (BI)

Revision as of 14:36, 15 August 2008

Contents

PCR products

PCR products
Number Name Oligo F Oligo R Template Length Comments Product Left ? []µg/µL Date
PCR124 pFlgA O100 O101 - 261pb yes 0.5 August 7
PCR125 pFlgB O102 O103 - 261pb yes 0.5 August 7
PCR126 pFlhB O108 O109 - 260pb yes 0.5 August 7
PCR127 flhDC O131 O132 MG1655 no August 8
PCR128 OmpR* O138 O139 Strain OmpR* 700pb yes 150 August 8
PCR129 EnvZ O126 O127 Strain EnvZ* 1400pb yes 100 August 8
PCR130 E0240 rbs+ O141 O140 MP143 900pb no August 11
PCR131 flhD rbs- O132 O133 MG1655 350pb no August 11
PCR132 pFlhD rbs- O130 O131 MG1655 600pb no August 11
PCR133 flhDC + prom O110 O131 MG1655 1300pb no August 11
PCR134 flhDC + prom O111 O131 MG1655 no August 11
PCR135 pfliL O124 O125 MG1655 yes August 11
PCR136 pFlhDC O110 O113 MG1655 yes August 11
PCR137 pFlhDC O111 O113 MG1655 yes August 11

Plasmids: MiniPrep Products

Plasmids: MiniPrep Products 2008
Number Name Biobricks Description Products in use Date
MP100 [http://partsregistry.org/Part:BBa_B0034 B0034 ] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.png Strongest RBS (Efficiency = 1) July 26
MP101 [http://partsregistry.org/Part:BBa_J23101 J23101] in [http://partsregistry.org/Part:BBa_J61002 pJ61002 ] Part icon regulatory.png Strongest Constitutive promoter July 26 July 22, July 23, July 26
MP102 [http://partsregistry.org/Part:BBa_J23109 J23109] in [http://partsregistry.org/Part:BBa_J61002 pJ61002 ] Part icon regulatory.png Weak Constitutive promoter July 26 July 22, July 26
MP103 [http://partsregistry.org/Part:BBa_R0079 R0079] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.png promoter pLas (lasr & PAI regulated) July 26 July 23, July 26
MP104 [http://partsregistry.org/Part:BBa_R0040 R0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.png tetR repressible promoter July 26 July 23, July 26
MP105 [http://partsregistry.org/Part:BBa_S03154 S03154] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.png B0034(rbs) ->LasI July 26
MP106 [http://partsregistry.org/Part:BBa_S03879 S03879] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.png B0034(rbs) -> TetR July 26
MP107 [http://partsregistry.org/Part:BBa_C0079 C0079] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] Icon coding.png lasR activator with LVA Tag July 23
MP108 [http://partsregistry.org/Part:BBa_C0179 C0179] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png lasR activator July 26 July 22, July 23, July 26
MP109 [http://partsregistry.org/Part:BBa_E0030 E0030] in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Part icon reporter.png YFP July 26
MP110 [http://partsregistry.org/Part:BBa_E0040 E0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon reporter.png GFP July 26 July 23, July 26
MP111 [http://partsregistry.org/Part:BBa_E1010 E1010 ] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] Part icon reporter.png mRFP July 26 July 23, July 26
MP112 OmpR M571 Icon coding.png July 23
MP113 EnvZ I2815 Icon coding.png July 23
MP114 [http://partsregistry.org/Part:BBa_C0040 C0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png tetR repressor July 23
MP115 [http://partsregistry.org/Part:pSB1A3 pSB1A3] Icon plasmid.png July 23
MP116 [http://partsregistry.org/Part:BBa_J23100 pJ23100] in [http://partsregistry.org/Part:BBa_J61002 J61002] Part icon regulatory.png Strong constitutive promoter in J61002 July 26
MP117 [http://partsregistry.org/Part:BBa_J23107 pJ23107] in [http://partsregistry.org/Part:BBa_J61002 J61002] Part icon regulatory.png Medium constitutive promoter in J61002 July 26 July 24, July 26
MP118 [http://partsregistry.org/Part:BBa_B0015 B0015] in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Part icon terminator.pngPart icon terminator.png Double terminator July 26 July 24, July 26
MP119 [http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] Part icon regulatory.png AraC pBAD July 26 July 24, July 26
MP120 [http://partsregistry.org/Part:BBa_B0030 B0030] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.png Strong RBS (Efficiency = 0,6) July 26
MP121 [http://partsregistry.org/Part:BBa_E0422 E0422] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.pngPart icon terminator.png ECFP (RBS+LVA+Term) July 26 July 24, July 26
MP122 [http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.pngPart icon terminator.png gfp tri-part; strong rbs July 26
MP123 [http://partsregistry.org/Part:J61002 J61002] Icon plasmid.png August 5
MP124 L102 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.png Strong rbs - YFP
D129 (BV) - D118 (BI) 		August 1
MP125 L103 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.png Strong rbs - mRFP
D129 (BV) - D122 (BI) 		August 1
MP126 L104 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.png Strong rbs - lasR activator
D129 (BV) - D114 (BI) 		August 1
MP127 L105 in [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png Strong promoter - ECFP
D123 (BV) - D130 (BI) 		August 1
MP128 L106 in [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png Strong promoter - gfp Tripart
D123 (BV) - D131 (BI) 		August 1
MP129 L107 in [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png Strong promoter - ECFP
D103 (BV) - D130 (BI) 		August 1
MP130 L108 in [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png Strong promoter - gfp Tripart
D103 (BV) - D131 (BI) 		August 1
MP131 L110 in [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png Medium promoter - gfp Tripart
D124 (BV) - D131 (BI) 		August 1
MP132 L111 in [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png Weak promoter - ECFP
D104 (BV) - D130 (BI) 		August 1
MP133 L112 in [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon reporter.png Weak promoter - GFP tripart
D104 (BV) - D131 (BI) 		August 1
MP134 L115 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png pLas - ECFP
D105 (BV) - D130 (BI) 		August 1
MP135 L116 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png pLas - gfp Tripart
D105 (BV) - D131 (BI) 		August 1
MP136 L117 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Part icon reporter.pngPart icon terminator.pngPart icon terminator.png yfp - Double Terminator
D125 (FV) - D117 (FI) 		August 1
MP137 L118 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Part icon reporter.pngPart icon terminator.pngPart icon terminator.png rfp - Double Terminator
D125 (FV) - D121 (FI) 		August 1
MP138 L119 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Icon coding.pngPart icon terminator.pngPart icon terminator.png lasR activator + LVA - Double Terminator
D125 (FV) - D113 (FI) 		August 1
MP139 L121 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png pTet - gfp tripart
D106 (BV) - D131 (BI) 		August 1
MP140 L124 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.png Strongest RBS - rfp
D102 (BV) - D122 (BI) 		August 1
MP141 L125 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.png Strongest RBS - yfp
D102 (BV) - D118 (BI) 		August 1
MP142 [http://partsregistry.org/Part:pSB3K3 pSB3K3] Icon plasmid.png August 8
MP143 [http://partsregistry.org/Part:E0240 E0240] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.pngPart icon terminator.png gfp generator August 8
MP144 L128 Part icon regulatory.pngPart icon reporter.png pFlgA-RFP
D132 (FI) - D136 (FV) 		MP144.3 ; MP144.4 August 12
MP145 L129 Part icon regulatory.pngPart icon reporter.png pFlgB-RFP
D133 (FI) - D136 (FV) 		MP145.6 ; MP145.7 August 12
MP146 L130 Part icon regulatory.pngPart icon reporter.png pFlhB-RFP
D134 (FI) - D136 (FV) 		MP146.1 ; MP146.2 August 12
MP147 L100 Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png rbs TetR - ECFP
D110 (BV) - D130 (BI) 		August 13
MP148 L101 Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png rbs TetR - GFP tripart
D110 (BV) - D131 (BI) 		MP148.2 ; MP148.3 August 13
MP149 L114 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png AracpBAD - gfp tripart
D126 (BV) - D131 (BI) 		August 13
MP150 L120 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png tetR repressible promoter - ECFP
D106 (BV) - D130 (BI) 		August 13
MP151 L122 Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png RBS-lasI - ECFP
D107 (BV) - D130 (BI) 		August 13
MP152 L123 Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png RBS lasI - gfp tripart
D107 (BV) - D131 (BI) 		August 13
MP153 L126 Part icon rbs.pngIcon coding.png Strongest RBS (1)- LasR activator (+LVA)
D102 (BV) - D114 (BI) 		MP153.1 ; MP153.3 August 13
MP154 L132 Icon coding.png flhDC
D142(FV) - D139(FI) 		August 13
MP155 L133 Icon coding.png OmpR*
D142(FV) - D140(FI) 		August 13
MP156 L134 Icon coding.png EnvZ*
D142(FV) - D141(FI) 		August 13
MP157 L138 Icon rbs.pngIcon reporter.pngPart icon terminator.png gfp generator (E0240)
D137(FV) - D138(FI) 		August 13
MP158 L139 August 15
MP159 L141 August 15
MP160 L142 August 15

Digestion Products

(Legend: Vector, Insert) Ø

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Obs Size Matrix Size BB Description Product Left []ng/µL Date
D100.1 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 34 10 July 26
D100.2 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 34 10 July 26
D101 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2076 15 10 July 29
D102.1 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2077 14 10 July 26
D102.2 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2077 14 10 July 29
D103.1 [http://partsregistry.org/Part:BBa_J23101 J23101] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2100 883 10 July 26
D103.2 [http://partsregistry.org/Part:BBa_J23101 J23101] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2100 883 10 July 26
D104.1 [http://partsregistry.org/Part:BBa_J23109 J23109] [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV 2100 883 20 July 26
D104.2 [http://partsregistry.org/Part:BBa_J23109 J23109] [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV 2100 883 20 July 26
D105.1 [http://partsregistry.org/Part:BBa_R0079 R0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2222 14 5 July 26
D105.2 [http://partsregistry.org/Part:BBa_R0079 R0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2222 14 5 July 29
D106.1 [http://partsregistry.org/Part:BBa_R0040 R0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2119 14 8 July 26
D106.2 [http://partsregistry.org/Part:BBa_R0040 R0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2119 14 5 July 29
D107 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2750 14 5 July 26
D108.1 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 707 5 July 26
D108.2 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 707 5 July 29
D109 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2056 708 5 July 26
D110 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2768 14 20 July 26
D111.1 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 725 20 July 26
D111.2 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 725 20 July 29
D112 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2756 726 20 July 26
D113 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] EcoRI SpeI FI 4402 779 20 July 26
D114 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] XbaI PstI BI 4003 778 20 July 26
D115.1 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 4402 746 - July 29
D115.2 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 4402 746 - July 29
D116.1 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 745 20 July 29
D116.1 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 745 20 July 29
D117 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI SpeI FI 3166 746 5 July 29
D118 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB2K3 pSB2K3] XbaI PstI BI 3167 745 20 July 26
D119 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2056 743 10 July 26
D120 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 742 10 July 26
D121 [http://partsregistry.org/Part:BBa_E1010 E1010] [http://partsregistry.org/Part:pSB2K3 pSB2K3] EcoRI SpeI FI 4402 704 10 July 26
D122 [http://partsregistry.org/Part:BBa_E1010 E1010] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 703 10 July 26
D123.1 [http://partsregistry.org/Part:BBa_J23100 J23100] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 10 July 26
D123.2 [http://partsregistry.org/Part:BBa_J23100 J23100] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 10 July 26
D124.1 [http://partsregistry.org/Part:BBa_J23107 J23107] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 15 July 26
D124.2 [http://partsregistry.org/Part:BBa_J23107 J23107] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 15 July 26
D125.1 [http://partsregistry.org/Part:BBa_B0015 B0015] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI XbaI FV 3303 15 15 July 26
D125.2 [http://partsregistry.org/Part:BBa_B0015 B0015] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI XbaI FV 3303 15 15 July 26
D126.1 [http://partsregistry.org/Part:BBa_I0500 I0500] [http://partsregistry.org/Part:pSB2K3 pSB2K3] SpeI PstI BV 5621 14 10 July 26
D126.2 [http://partsregistry.org/Part:BBa_I0500 I0500] [http://partsregistry.org/Part:pSB2K3 pSB2K3] SpeI PstI BV 5621 14 10 July 26
D127.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 37 10 July 26
D127.2 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 37 10 July 26
D128.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2079 15 10 July 29
D128.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2079 15 10 July 29
D129.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2080 14 10 July 29
D129.2 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2080 14 10 July 29
D130.1 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 939 10 July 26
D130.2 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 939 10 July 26
D130.3 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 939 10 July 26
D131.1 [http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 900 10 July 26
D131.2 [http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 900 10 July 26
D131.3 [http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 900 10 July 26
D132 pFlgA - EcoRI SpeI FI - 250 5 August 6
D133 pFlgB - EcoRI SpeI FI - 250 5 August 6
D134 pFlhB - EcoRI SpeI FI - 249 5 August 6
D135 pFlhDC - EcoRI SpeI FI - problem August 6
D136 - [http://partsregistry.org/Part:BBa_J61002 J61002] EcoRI SpeI FV 2925 - August 6
D137 pSB3K3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] EcoRI SpeI FV 2727 - August 6
D138 E0240 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 899 - August 6
D139 flhDC - EcoRI PstI FI - 972 August 6
D140 E040 rbs+ - PstI PstI I - August 12
D141 flhD RBS - - EcoRI SpeI FI - August 12
D142 flhC RBS - - EcoRI SpeI FI - August 12
D143 flhDC + promoter - EcoRI SpeI FI - August 12
D144 pSB3K3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] EcoRI SpeI FI - August 12
D145 pSB1A2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI - August 12
D146 MP148 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI 2057 1609 rbs TetR - GFP tripart
D110 (BV) - D131 (BI)
Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png 		August 15
D147 MP153 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 2056 799 Strongest RBS (1)- LasR activator (+LVA)
D102 (BV) - D114 (BI)
Part icon rbs.pngIcon coding.png 		August 15
D149 pfliL - EcoRI SpeI FI - August 14
D150 pfliL - XbaI PstI BI - August 14
D152 pSB3K3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] XbaI PstI BI - August 14

Strains (glycerol stock)

Strains glycerol stock
Number Name Biobricks Description Verified clone Date
S103 [http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3] Part icon regulatory.png AraC pBAD July 23
S106 [http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.pngPart icon terminator.png ECFP (RBS+LVA+Term July 23
S107 [http://partsregistry.org/Part:BBa_B0034 B0034] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.png Strongest RBS (Efficiency = 1) July 23
S108 [http://partsregistry.org/Part:BBa_R0079 R0079] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.png promoter pLas (lasr & PAI regulated) July 23
S109 [http://partsregistry.org/Part:BBa_J23101 J23101] in [http://partsregistry.org/Part:BBa_J61002 pJ61002 ] Part icon regulatory.png Strongest Constitutive promoter July 23
S110 [http://partsregistry.org/Part:BBa_J23109 J23109] in [http://partsregistry.org/Part:BBa_J61002 pJ61002 ] Part icon regulatory.png Weak Constitutive promoter July 23
S111 [http://partsregistry.org/Part:BBa_R0040 R0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.png tetR repressible promoter July 23
S112 [http://partsregistry.org/Part:BBa_S03154 S03154] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.png B0034(rbs) ->LasI July 23
S113 [http://partsregistry.org/Part:BBa_S03879 S03879] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.png B0034(rbs) -> TetR July 23
S114 [http://partsregistry.org/Part:BBa_C0079 C0079] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] Icon coding.png lasR activator with LVA Tag July 23
S115 [http://partsregistry.org/Part:BBa_C0179 C0179] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png lasR activator July 23
S116 [http://partsregistry.org/Part:BBa_E0030 E0030] in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Part icon reporter.png YFP July 23
S117 [http://partsregistry.org/Part:BBa_E0040 E0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon reporter.png GFP July 23
S118 [http://partsregistry.org/Part:BBa_E1010 E1010] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] Part icon reporter.png mRFP July 23
S119 OmpR M571 Icon coding.png July 23
S120 EnvZ I2815 Icon coding.png July 23
S121 [http://partsregistry.org/Part:BBa_C0040 C0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png tetR repressor July 23
S122 [http://partsregistry.org/Part:pSB1A3 pSB1A3] Icon plasmid.png July 23
S123 L102 Part icon rbs.pngPart icon reporter.png Strong rbs - YFP
D129 (BV) - D118 (BI) 		Aug 1
S124 L103 Part icon rbs.pngPart icon reporter.png Strong rbs - mRFP
D129 (BV) - D122 (BI) 		Aug 1
S125 L104 Part icon rbs.pngIcon coding.png Strong rbs - lasR activator
D129 (BV) - D114 (BI) 		Aug 1
S126 L105 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png Strong promoter - ECFP
D123 (BV) - D130 (BI) 		Aug 1
S127 L106 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png Strong promoter - gfp Tripart
D123 (BV) - D131 (BI) 		Aug 1
S128 L107 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png Strongest promoter - ECFP
D103 (BV) - D130 (BI) 		Aug 1
S129 L108 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png Strong promoter - GFP tripart
D103 (BV) - D131 (BI) 		Aug 1
S130 L110 Part icon regulatory.pngPart icon reporter.png Medium promoter - gfp tripart
D124 (BV) - D131 (BI) 		Aug 1
S131 L111 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png Weak promoter - ECFP
D104 (BV) - D130 (BI) 		Aug 1
S132 L112 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png Weak promoter - gfp Tripart
D104 (BV) - D131 (BI) 		Aug 1
S133 L115 Part icon reporter.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png pLas - ECFP
D105 (BV) - D130 (BI) 		Aug 1
S134 L116 Part icon reporter.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png pLas - gfp Tripart
D105 (BV) - D131 (BI) 		Aug 1
S135 L117 Part icon reporter.pngPart icon terminator.pngPart icon terminator.png yfp - Double Terminator
D125 (FV) - D117 (FI) 		Aug 1
S136 L118 Part icon reporter.pngPart icon terminator.pngPart icon terminator.png rfp - Double Terminator
D125 (FV) - D121 (FI) 		Aug 1
S137 L119 Icon coding.pngPart icon terminator.pngPart icon terminator.png LasR activator + LVA - Double terminator
D125 (FV) - D113 (FI) 		Aug 1
S138 L121 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png pTet - gfp Tripart
D106 (BV) - D131 (BI) 		Aug 1
S139 L124 Part icon rbs.pngPart icon reporter.png Strongest rbs - mRFP
D102 (BV) - D122 (BI) 		Aug 1
S140 L125 Part icon rbs.pngPart icon reporter.png Strongest rbs - yfp
D102 (BV) - D118 (BI) 		Aug 1
S141 MG1655 Aug 7
S142 [http://partsregistry.org/Part:J61002 J61002] Icon plasmid.png Aug 7
S143 L128 Part icon regulatory.pngPart icon reporter.png pFlgA-RFP
D132 (FI) - D136 (FV) 		MP144.3 ; MP144.4 August 12
S144 L129 Part icon regulatory.pngPart icon reporter.png pFlgB-RFP
D133 (FI) - D136 (FV) 		MP145.6 ; MP145.7 August 12
S145 L130 Part icon regulatory.pngPart icon reporter.png pFlhB-RFP
D134 (FI) - D136 (FV) 		MP146.1 ; MP146.2 August 12
S146 L100 Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png rbs TetR - ECFP
D110 (BV) - D130 (BI) 		August 13
S147 L101 Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png rbs TetR - GFP tripart
D110 (BV) - D131 (BI) 		August 13
S148 L114 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png AracpBAD - gfp tripart
D126 (BV) - D131 (BI) 		August 13
S149 L120 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png tetR repressible promoter - ECFP
D106 (BV) - D130 (BI) 		August 13
S150 L122 Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png RBS-lasI - ECFP
D107 (BV) - D130 (BI) 		August 13
S151 L123 Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png RBS lasI - gfp tripart
D107 (BV) - D131 (BI) 		August 13
S152 L126 Part icon rbs.pngIcon coding.png Strongest RBS (1)- LasR activator (+LVA)
D102 (BV) - D114 (BI) 		August 13
S153 L132 Icon coding.png flhDC
D142(FV) - D139(FI) 		August 13
S154 L133 Icon coding.png OmpR*
D142(FV) - D140(FI) 		August 13
S155 L134 Icon coding.png EnvZ*
D142(FV) - D141(FI) 		August 13
S156 L138 Icon rbs.pngIcon reporter.pngPart icon terminator.png gfp generator (E0240)
D137(FV) - D138(FI) 		August 13
S157 August 15
S158 August 15
S159 L139 August 15
S160 L141 August 15
S161 L142 August 15
Retrieved from "http://2008.igem.org/Team:Paris/Notebook/Freezer"