"
Team:Hawaii/Experiment
From 2008.igem.org
(Difference between revisions)
(→In Progress: deleted experiment) |
|||
| Line 5: | Line 5: | ||
* [[Team:Hawaii/PCC6803 Growth Optimization | PCC6803 Growth Optimization]] | * [[Team:Hawaii/PCC6803 Growth Optimization | PCC6803 Growth Optimization]] | ||
* [[Team:Hawaii/Ligation of pRL1383a Parts| Ligation of pRL1383a Parts]] | * [[Team:Hawaii/Ligation of pRL1383a Parts| Ligation of pRL1383a Parts]] | ||
| - | |||
* [[Team:Hawaii/Construction of BioBrick intermediates|Construction of BioBrick intermediates]] | * [[Team:Hawaii/Construction of BioBrick intermediates|Construction of BioBrick intermediates]] | ||
* [[Team:Hawaii/Construction of Broad-Host-Range Expression Vector| Construction of Broad-Host-Range Expression Vector]] | * [[Team:Hawaii/Construction of Broad-Host-Range Expression Vector| Construction of Broad-Host-Range Expression Vector]] | ||
| Line 33: | Line 32: | ||
* [[Team:Hawaii/Large-Scale Preparation of Plasmid from E. coli | Large-Scale Preparation of Plasmid]] | * [[Team:Hawaii/Large-Scale Preparation of Plasmid from E. coli | Large-Scale Preparation of Plasmid]] | ||
* [[Team:Hawaii/Initial_Synth._Oligo_Assembly| Initial Synthetic Oligonucleotide Assembly]] | * [[Team:Hawaii/Initial_Synth._Oligo_Assembly| Initial Synthetic Oligonucleotide Assembly]] | ||
| + | * [[Team:Hawaii/Biobrick_conversions|Conversion of GFP into a fusion brick and pRL1383a into a Biobrick vector]] | ||
{{Team:Hawaii/Footer}} | {{Team:Hawaii/Footer}} | ||
__NOTOC__ | __NOTOC__ | ||
Revision as of 23:23, 16 August 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
In Progress
- PCC6803 Growth Optimization
- Ligation of pRL1383a Parts
- Construction of BioBrick intermediates
- Construction of Broad-Host-Range Expression Vector
- Construction of Omega Interposon BioBrick
Not Started
Completed
- PCR Amplification of pRL1383a
- PCC6803 cell density test
- PCC6803 Cryostock Thaw Test
- Effect of CO2 on Growth Experiment
- Effect of Water Motion on Clumping of Liquid Cultures
- Make Competent E. Coli
- Test Competent E. Coli
- Initial E. Coli Transformation
- Initial BioBrick Plasmid Extraction From Filter Paper
- Biobricks extraction/transformation competency test
- Initial PCC6803 Transformation
- Tri-parental conjugation between E. coli and PCC6803
- Mini Plasmid Prep
- Large-Scale Preparation of Plasmid
- Initial Synthetic Oligonucleotide Assembly
- Conversion of GFP into a fusion brick and pRL1383a into a Biobrick vector
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
