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Team:KULeuven/30 August 2008
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=== Wet Lab === | === Wet Lab === | ||
| - | * A glycerol stock was made of the new parts. | + | * A glycerol stock was made of the new parts and of our own parts (K145151, K145001 and K145015). |
* We MiniPrepped the new parts and the two ligations that succeeded (C0040+B0015 and C0060+B0015). | * We MiniPrepped the new parts and the two ligations that succeeded (C0040+B0015 and C0060+B0015). | ||
* The digestions with ''Xba''I were started. | * The digestions with ''Xba''I were started. | ||
Revision as of 09:55, 31 August 2008
dock/undock dropdown
Contents |
Lab Work
Wet Lab
- A glycerol stock was made of the new parts and of our own parts (K145151, K145001 and K145015).
- We MiniPrepped the new parts and the two ligations that succeeded (C0040+B0015 and C0060+B0015).
- The digestions with XbaI were started.
- The ligations we made yesterday were electroporated today (still in electrocompetent Top10 cells).
- A liquid culture was made of part R0053.
Dry Lab
Created the oligos for the B1002 and B1006 terminators [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=terminator link]
Modeling
Final updated model uploaded to the wiki (only MatLab)
Wiki
Remarks
I don't think the dam methylation is the problem with our ligations. This is the prefix: gaattcgcggccgcttctagag. The recognition site of XbaI is in bold. There is no overlap for dam methylation (gatc).
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