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Team:UNIPV-Pavia/Notebook/Week14
From 2008.igem.org
(Difference between revisions)
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**Lig.22 - point mutation C->T | **Lig.22 - point mutation C->T | ||
**Lig.30 - point mutation C->T | **Lig.30 - point mutation C->T | ||
| - | *We decided to restart from Lig.12, whose sequence was correct, to perform | + | *We decided to restart from Lig.12, whose sequence was correct, to perform ligations Lig.22, Lig.30, Lig.34 and Lig.35. |
*We prepared 0.5 l of LB + Amp for plates. | *We prepared 0.5 l of LB + Amp for plates. | ||
| - | *We infected 9 ml LB + Amp with 30 µl of B0030-C0061-B1006-R0062-B0030-E0040-B1006 and Lig.12 glycerol stocks. | + | *We infected 9 ml LB + Amp with 30 µl of B0030-C0061-B1006-'''R0062'''-B0030-E0040-B1006 and Lig.12 glycerol stocks. |
'''08/22/08''' | '''08/22/08''' | ||
<br> | <br> | ||
| - | *Glycerol stocks/miniprep for B0030-C0061-B1006-R0062-B0030-E0040-B1006 and Lig.12. | + | *Glycerol stocks/miniprep for B0030-C0061-B1006-'''R0062'''-B0030-E0040-B1006 and Lig.12. |
*We planned experiments for qualitative tests. | *We planned experiments for qualitative tests. | ||
Revision as of 18:07, 26 October 2008
 Home
|  The Team
|  The Project
|  Biological Safety
|  Parts Submitted to the Registry
|
|---|---|---|---|---|
 Dry Lab
|  Wet Lab
|  Modeling
|  Protocols
|  Activity Notebook
|
Notebook
| Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 |
|---|---|---|---|---|---|---|
| Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 |
| Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20 | Week 21 |
Week 11: 08/21/08 - 08/22/08
08/21/08
- Sequencing results:
- J23100-B0030-C0012-B1006-R0010 - completely wrong sequence
- C0062 - OK
- Lig.12 - OK
- Lig.22 - point mutation C->T
- Lig.30 - point mutation C->T
- We decided to restart from Lig.12, whose sequence was correct, to perform ligations Lig.22, Lig.30, Lig.34 and Lig.35.
- We prepared 0.5 l of LB + Amp for plates.
- We infected 9 ml LB + Amp with 30 µl of B0030-C0061-B1006-R0062-B0030-E0040-B1006 and Lig.12 glycerol stocks.
08/22/08
- Glycerol stocks/miniprep for B0030-C0061-B1006-R0062-B0030-E0040-B1006 and Lig.12.
- We planned experiments for qualitative tests.
