Team:Paris/Notebook/Freezer

From 2008.igem.org

(Difference between revisions)
(→rebut)
(→Biobricks)
Line 368: Line 368:
{|
{|
|- style="background: #649CD7;"  
|- style="background: #649CD7;"  
-
| colspan="8" style="background: #649CD7;" | BioBricks
+
! colspan="8" style="background: #649CD7;" | Ligations: Constructions realised
|- style="background: #649CD7; text-align: center;"
|- style="background: #649CD7; text-align: center;"
-
|width=10%| Name/N°
+
|width=8%| Name/N°
-
|width=10%| Plasmid
+
|width=8%| Plasmid
-
|width=10%| Strain
+
|width=7%| Strain
-
|width=10%| Miniprep
+
|width=7%| Miniprep
-
|width=30%|Description
+
|width=32%|Description
-
|width=20%| comment
+
|width=20%| Digestion
 +
|width=8%| comment
|width=10%| Date
|width=10%| Date
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 383: Line 384:
|align="center"|
|align="center"|
|align="center"|Strain MG1655<br>[[Image:Icon_coding.png]]<br>EnvZ*
|align="center"|Strain MG1655<br>[[Image:Icon_coding.png]]<br>EnvZ*
 +
|
|
|
|align="center"|[[Team:Paris/July 23|July 23]]
|align="center"|[[Team:Paris/July 23|July 23]]
Line 391: Line 393:
|align="center"|
|align="center"|
|align="center"|Strain MG1655<br>[[Image:Icon_coding.png]]<br>OmpR*
|align="center"|Strain MG1655<br>[[Image:Icon_coding.png]]<br>OmpR*
 +
|
|align="center"|Really mutated?
|align="center"|Really mutated?
|align="center"|[[Team:Paris/July 23|July 23]]
|align="center"|[[Team:Paris/July 23|July 23]]
Line 400: Line 403:
|align="center"|Strain
|align="center"|Strain
|align="center" |
|align="center" |
 +
|
|align="center" |[[Team:Paris/August 7|Aug 7]]
|align="center" |[[Team:Paris/August 7|Aug 7]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 407: Line 411:
|align="center"|
|align="center"|
|align="center"|Strain MG1655
|align="center"|Strain MG1655
 +
|
|align="center"|Δ FliA Frt
|align="center"|Δ FliA Frt
|align="center"|[[Team:Paris/October 25|October 25]]
|align="center"|[[Team:Paris/October 25|October 25]]
Line 415: Line 420:
|align="center"|
|align="center"|
|align="center"|Strain MG1655
|align="center"|Strain MG1655
 +
|
|align="center"|Δ FlhD Frt
|align="center"|Δ FlhD Frt
|align="center"|[[Team:Paris/October 25|October 25]]
|align="center"|[[Team:Paris/October 25|October 25]]
Line 423: Line 429:
|align="center"|
|align="center"|
|align="center"|Strain MG1655
|align="center"|Strain MG1655
 +
|
|align="center"|Δ FlgM Frt
|align="center"|Δ FlgM Frt
|align="center"|[[Team:Paris/October 25|October 25]]
|align="center"|[[Team:Paris/October 25|October 25]]
Line 431: Line 438:
|align="center"|
|align="center"|
|align="center"|Strain MG1655
|align="center"|Strain MG1655
 +
|
|align="center"|Δ EnvZ :: Cm
|align="center"|Δ EnvZ :: Cm
|align="center"|[[Team:Paris/October 25|October 25]]
|align="center"|[[Team:Paris/October 25|October 25]]
Line 440: Line 448:
|align="center"|MP123
|align="center"|MP123
|align="center"|[[Image:Icon_plasmid.png]]<br>plasmid
|align="center"|[[Image:Icon_plasmid.png]]<br>plasmid
 +
|
|
|
|align="center"|[[Team:Paris/August 5| August 5 ]]
|align="center"|[[Team:Paris/August 5| August 5 ]]
Line 448: Line 457:
|align="center"|MP115
|align="center"|MP115
|align="center"|[[Image:Icon_plasmid.png]]<br>plasmid
|align="center"|[[Image:Icon_plasmid.png]]<br>plasmid
 +
|
|
|
|align="center"|[[Team:Paris/July 23|July 23]]
|align="center"|[[Team:Paris/July 23|July 23]]
Line 456: Line 466:
|align="center"|MP215
|align="center"|MP215
|align="center"|[[Image:icon_plasmid.png]]<br>plasmid
|align="center"|[[Image:icon_plasmid.png]]<br>plasmid
 +
|
|align="center"|low copy plasmid with pSC101 ORI
|align="center"|low copy plasmid with pSC101 ORI
|align="center"|[[Team:Paris/October 14|October 14]]
|align="center"|[[Team:Paris/October 14|October 14]]
Line 464: Line 475:
|align="center"|MP142
|align="center"|MP142
|align="center"|[[Image:Icon_plasmid.png]]<br>plasmid
|align="center"|[[Image:Icon_plasmid.png]]<br>plasmid
 +
|
|align="center"|low to medium copy plasmid with p15A ORI  
|align="center"|low to medium copy plasmid with p15A ORI  
|align="center"|[[Team:Paris/August 15|August 15]]
|align="center"|[[Team:Paris/August 15|August 15]]
Line 472: Line 484:
|align="center"|MP225
|align="center"|MP225
|align="center"|[[Image:Icon_plasmid.png]]<br>plasmid
|align="center"|[[Image:Icon_plasmid.png]]<br>plasmid
 +
|
|align="center"|FliA in pBad18 (ORI pBR  / ampicillin resistance)
|align="center"|FliA in pBad18 (ORI pBR  / ampicillin resistance)
|align="center"|[[Team:Paris/October 23|October 23]]
|align="center"|[[Team:Paris/October 23|October 23]]
Line 480: Line 493:
|align="center"|MP217
|align="center"|MP217
|align="center"|[[Image:Icon_plasmid.png]]<br>plasmid
|align="center"|[[Image:Icon_plasmid.png]]<br>plasmid
 +
|
|align="center"|FlhDC in pBad18 (ORI pBR / ampicillin resistance)
|align="center"|FlhDC in pBad18 (ORI pBR / ampicillin resistance)
|align="center"|[[Team:Paris/October 24|October 24]]
|align="center"|[[Team:Paris/October 24|October 24]]
Line 488: Line 502:
|align="center"|MP116
|align="center"|MP116
|align="center"|[[Image:Part_icon_regulatory.png]]<br>Strong constitutive promoter
|align="center"|[[Image:Part_icon_regulatory.png]]<br>Strong constitutive promoter
 +
|
|
|
|align="center"|[[Team:Paris/July 26|July 26]]
|align="center"|[[Team:Paris/July 26|July 26]]
Line 496: Line 511:
|align="center"|MP101
|align="center"|MP101
|align="center"|[[Image:Part_icon_regulatory.png]]<br>Strongest Constitutive promoter
|align="center"|[[Image:Part_icon_regulatory.png]]<br>Strongest Constitutive promoter
 +
|
|
|
|align="center"|[[Team:Paris/July 22|July 22]], [[Team:Paris/July 23|July 23]], [[Team:Paris/July 26|July 26]]
|align="center"|[[Team:Paris/July 22|July 22]], [[Team:Paris/July 23|July 23]], [[Team:Paris/July 26|July 26]]
Line 504: Line 520:
|align="center"|MP117
|align="center"|MP117
|align="center"|[[Image:Part_icon_regulatory.png]]<br>Medium constitutive promoter
|align="center"|[[Image:Part_icon_regulatory.png]]<br>Medium constitutive promoter
 +
|
|
|
|align="center"|[[Team:Paris/July 24|July 24]], [[Team:Paris/July 26|July 26]]
|align="center"|[[Team:Paris/July 24|July 24]], [[Team:Paris/July 26|July 26]]
Line 512: Line 529:
|align="center"|MP102
|align="center"|MP102
|align="center"|[[Image:Part_icon_regulatory.png]]<br>Weak Constitutive promoter
|align="center"|[[Image:Part_icon_regulatory.png]]<br>Weak Constitutive promoter
 +
|
|
|
|align="center"|[[Team:Paris/July 22|July 22]], [[Team:Paris/July 26|July 26]]
|align="center"|[[Team:Paris/July 22|July 22]], [[Team:Paris/July 26|July 26]]
Line 520: Line 538:
|align="center"|MP119
|align="center"|MP119
|align="center"|[[Image:Part_icon_regulatory.png]]<br>AraC pBAD
|align="center"|[[Image:Part_icon_regulatory.png]]<br>AraC pBAD
 +
|
|
|
|align="center"|[[Team:Paris/July 24|July 24]], [[Team:Paris/July 26|July 26]]
|align="center"|[[Team:Paris/July 24|July 24]], [[Team:Paris/July 26|July 26]]
Line 528: Line 547:
|align="center"|MP104
|align="center"|MP104
|align="center"|[[Image:Part_icon_regulatory.png]]<br>tetR repressible promoter
|align="center"|[[Image:Part_icon_regulatory.png]]<br>tetR repressible promoter
 +
|
|
|
|align="center"|[[Team:Paris/July 23|July 23]], [[Team:Paris/July 26|July 26]]
|align="center"|[[Team:Paris/July 23|July 23]], [[Team:Paris/July 26|July 26]]
Line 536: Line 556:
|align="center"|MP103
|align="center"|MP103
|align="center"|[[Image:Part_icon_regulatory.png]]<br>promoter pLas (lasr & PAI regulated)
|align="center"|[[Image:Part_icon_regulatory.png]]<br>promoter pLas (lasr & PAI regulated)
 +
|
|
|
|align="center"|[[Team:Paris/July 23|July 23]], [[Team:Paris/July 26|July 26]]
|align="center"|[[Team:Paris/July 23|July 23]], [[Team:Paris/July 26|July 26]]
-
|- style="background: #dddddd;"
 
-
|style="background: #D4E2EF;" |L135
 
-
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|[[Image:Part_icon_regulatory.png]]<br>pFlgA
 
-
|align="center"|D137 (BV) - D133 (BI)<br>don't work
 
-
|align="center"|[[Team:Paris/August 9|August 9]]
 
-
|- style="background: #dddddd;" 
 
-
| style="background: #D4E2EF;" |L136
 
-
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|[[Image:Part_icon_regulatory.png]]<br>pFlgB
 
-
|align="center"|D137 (BV) - D1343 (BI)<br>don't work
 
-
|align="center"|[[Team:Paris/August 9|August 9]]
 
-
|- style="background: #dddddd;" 
 
-
| style="background: #D4E2EF;" |L137
 
-
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|[[Image:Part_icon_regulatory.png]]<br>pFlhB
 
-
|align="center"|D137 (BV) - D135 (BI)<br>don't work
 
-
|align="center"|[[Team:Paris/August 9|August 9]]
 
-
|- style="background: #dddddd;" 
 
-
| style="background: #D4E2EF;" |L147
 
-
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
 
-
|align="center"| x
 
-
|align="center"| x
 
-
|align="center"|[[Image:Icon_regulatory.png]]<br>pFlhDC
 
-
|align="center"|D155 - D136<br>didn't work
 
-
|align="center"|[[Team:Paris/August 16|August 16 ]]
 
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |L  
| style="background: #D4E2EF;" |L  
Line 576: Line 565:
|align="center"|MP207
|align="center"|MP207
|align="center"|[[Image:Icon_regulatory.png]]<br>pFliA
|align="center"|[[Image:Icon_regulatory.png]]<br>pFliA
 +
|
|
|
|align="center"|[[Team:Paris/October 04|October 04]]
|align="center"|[[Team:Paris/October 04|October 04]]
-
|- style="background: #dddddd;" 
 
-
| style="background: #D4E2EF;" |L143
 
-
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
 
-
|align="center"| x
 
-
|align="center"| x
 
-
|align="center"|[[Image:Icon_regulatory.png]]<br>pFliL
 
-
|align="center"|D149 - D137<br>didn't work
 
-
|align="center"|[[Team:Paris/August 14|August 14 ]]
 
-
|- style="background: #dddddd;" 
 
-
| style="background: #D4E2EF;" |L144
 
-
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
 
-
|align="center"| x
 
-
|align="center"| x
 
-
|align="center"|[[Image:Icon_regulatory.png]]<br>pFliL
 
-
|align="center"|D150 - D152<br>didn't work
 
-
|align="center"|[[Team:Paris/August 14|August 14 ]]
 
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 602: Line 576:
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlgA(RBS WT)
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlgA(RBS WT)
|  
|  
 +
|
|align="center"|[[Team:Paris/October 10|October 10]]
|align="center"|[[Team:Paris/October 10|October 10]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 609: Line 584:
|align="center"|MP184
|align="center"|MP184
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlgB-RBS (WT)
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlgB-RBS (WT)
 +
|
|  
|  
|align="center"|[[Team:Paris/September 04|September 04]]
|align="center"|[[Team:Paris/September 04|September 04]]
Line 617: Line 593:
|align="center"|MP175
|align="center"|MP175
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlhB-RBS (WT)
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlhB-RBS (WT)
 +
|
|  
|  
|align="center"|[[Team:Paris/September 04|September 04]]
|align="center"|[[Team:Paris/September 04|September 04]]
Line 625: Line 602:
|align="center"|MP212
|align="center"|MP212
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlhB(RBS WT)
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlhB(RBS WT)
 +
|
|  
|  
|align="center"|[[Team:Paris/October 10|October 10]]
|align="center"|[[Team:Paris/October 10|October 10]]
Line 633: Line 611:
|align="center"|MP177
|align="center"|MP177
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlhDC-RBS (WT)
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFlhDC-RBS (WT)
 +
|
|  
|  
|align="center"|[[Team:Paris/September 04|September 04]]
|align="center"|[[Team:Paris/September 04|September 04]]
-
 
-
 
-
 
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |L  
| style="background: #D4E2EF;" |L  
Line 644: Line 620:
|align="center"|MP176
|align="center"|MP176
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFliA-RBS (WT)  
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFliA-RBS (WT)  
 +
|
|align="center"|pFliA with conserved Pst1 site
|align="center"|pFliA with conserved Pst1 site
|align="center"|[[Team:Paris/September 04|September 04]]
|align="center"|[[Team:Paris/September 04|September 04]]
Line 653: Line 630:
|align="center"|MP178
|align="center"|MP178
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFliL-RBS (WT)
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFliL-RBS (WT)
 +
|
|  
|  
|align="center"|[[Team:Paris/September 04|September 04]]
|align="center"|[[Team:Paris/September 04|September 04]]
Line 661: Line 639:
|align="center"|MP210
|align="center"|MP210
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFliL(RBS WT)
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]]<br>pFliL(RBS WT)
 +
|
|  
|  
|align="center"|[[Team:Paris/October 10|October 10]]
|align="center"|[[Team:Paris/October 10|October 10]]
Line 670: Line 649:
|align="center"|[[Image:Part_icon_rbs.png]]<br>Strong RBS (Efficiency = 0,6)
|align="center"|[[Image:Part_icon_rbs.png]]<br>Strong RBS (Efficiency = 0,6)
|
|
 +
|
|align="center"|[[Team:Paris/July 26|July 26]]
|align="center"|[[Team:Paris/July 26|July 26]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 678: Line 658:
|align="center"|[[Image:Icon_rbs.png]]<br> medium rbs (efficiency = 0,3)
|align="center"|[[Image:Icon_rbs.png]]<br> medium rbs (efficiency = 0,3)
|
|
 +
|
|align="center"|[[Team:Paris/August 20|August 20]]
|align="center"|[[Team:Paris/August 20|August 20]]
|- style="background: #dddddd;"  
|- style="background: #dddddd;"  
Line 686: Line 667:
|align="center"|[[Image:Part_icon_rbs.png]]<br>Strongest RBS (Efficiency = 1)
|align="center"|[[Image:Part_icon_rbs.png]]<br>Strongest RBS (Efficiency = 1)
|
|
 +
|
|align="center"|[[Team:Paris/July 26|July 26]]
|align="center"|[[Team:Paris/July 26|July 26]]
-
 
-
 
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_S03154 S03154]
| style="background: #D4E2EF;" |[http://partsregistry.org/Part:BBa_S03154 S03154]
Line 696: Line 676:
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>B0034(rbs) ->LasI
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>B0034(rbs) ->LasI
|
|
 +
|
|align="center"|[[Team:Paris/July 26|July 26]]
|align="center"|[[Team:Paris/July 26|July 26]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 704: Line 685:
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>B0034(rbs) -> TetR  
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>B0034(rbs) -> TetR  
|
|
 +
|
|align="center"|[[Team:Paris/August 24|August 24]]
|align="center"|[[Team:Paris/August 24|August 24]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 711: Line 693:
|align="center"|MP179
|align="center"|MP179
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>RBS(FlhDC) - FlhDC  
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>RBS(FlhDC) - FlhDC  
 +
|
|align="center"|FlhDC with conserved Pst1 site
|align="center"|FlhDC with conserved Pst1 site
|align="center"|[[Team:Paris/September 04|September 04]]
|align="center"|[[Team:Paris/September 04|September 04]]
Line 720: Line 703:
|align="center"|[[Image:Icon_coding.png]]<br>tetR repressor  
|align="center"|[[Image:Icon_coding.png]]<br>tetR repressor  
|
|
 +
|
|align="center"|[[Team:Paris/July 23|July 23]]
|align="center"|[[Team:Paris/July 23|July 23]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 728: Line 712:
|align="center"|[[Image:Icon_coding.png]]<br>lasR activator with LVA Tag
|align="center"|[[Image:Icon_coding.png]]<br>lasR activator with LVA Tag
|
|
 +
|
|align="center"|[[Team:Paris/July 23|July 23]]
|align="center"|[[Team:Paris/July 23|July 23]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 736: Line 721:
|align="center"|[[Image:Icon_coding.png]]<br>lasR activator
|align="center"|[[Image:Icon_coding.png]]<br>lasR activator
|
|
 +
|
|align="center"|[[Team:Paris/July 22|July 22]], [[Team:Paris/July 23|July 23]], [[Team:Paris/July 26|July 26]]
|align="center"|[[Team:Paris/July 22|July 22]], [[Team:Paris/July 23|July 23]], [[Team:Paris/July 26|July 26]]
Line 746: Line 732:
|align="center"|[[Image:Icon_coding.png]]<br>flhDC
|align="center"|[[Image:Icon_coding.png]]<br>flhDC
|align="center"|D142(FV) - D139(FI)  
|align="center"|D142(FV) - D139(FI)  
 +
|
|align="center"|[[Team:Paris/August 13|August 13]]
|align="center"|[[Team:Paris/August 13|August 13]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 753: Line 740:
|align="center"|MP155
|align="center"|MP155
|align="center"|[[Image:Icon_coding.png]]<BR>OmpR*
|align="center"|[[Image:Icon_coding.png]]<BR>OmpR*
-
|align="center"|D142(FV) - D140(FI)<br>WT sequence  
+
|align="center"|D142(FV) - D140(FI)
 +
|align="center"|WT sequence  
|align="center"| [[Team:Paris/August 19|August 19]]
|align="center"| [[Team:Paris/August 19|August 19]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 761: Line 749:
|align="center"|MP156
|align="center"|MP156
|align="center"|[[Image:Icon_coding.png]]<BR> EnvZ*
|align="center"|[[Image:Icon_coding.png]]<BR> EnvZ*
-
|align="center"|D142(FV) - D141(FI)<br>lacks about 300 bp in EnvZ*
+
|align="center"|D142(FV) - D141(FI)
 +
|align="center"lacks about 300 bp in EnvZ*
|align="center"|[[Team:Paris/August 19| August 19]]
|align="center"|[[Team:Paris/August 19| August 19]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 770: Line 759:
|align="center"|[[Image:Icon_coding.png]]<br>flhD
|align="center"|[[Image:Icon_coding.png]]<br>flhD
|align="center"|D145 (V) - D141 (I)
|align="center"|D145 (V) - D141 (I)
 +
|align="center"
|align="center"|[[Team:Paris/August 12|August 12]]
|align="center"|[[Team:Paris/August 12|August 12]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 777: Line 767:
|align="center"|MP159
|align="center"|MP159
|align="center"|[[Image:Icon_coding.png]]<br>flhC
|align="center"|[[Image:Icon_coding.png]]<br>flhC
-
|align="center"|D142 (ES) - D145 (ES)<br>has not been sequenced
+
|align="center"|D142 (ES) - D145 (ES)
 +
|align="center"has not been sequenced
|align="center"|[[Team:Paris/August 15|August 15]]
|align="center"|[[Team:Paris/August 15|August 15]]
-
|- style="background: #dddddd;" 
+
 
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |L145
+
-
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
-
|align="center"| x
+
-
|align="center"| x
+
-
|align="center"|[[Image:Icon_coding.png]]<br>FlhDC
+
-
|align="center"|D1543 - D145<br>didn't work
+
-
|align="center"|[[Team:Paris/August 16|August 16 ]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |L146
+
-
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
-
|align="center"| x
+
-
|align="center"| x
+
-
|align="center"|[[Image:Icon_coding.png]]<br>FlhDC
+
-
|align="center"|D154 - D145<br>didn't work
+
-
|align="center"|[[Team:Paris/August 16|August 16 ]]
+
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |L  
| style="background: #D4E2EF;" |L  
Line 802: Line 777:
|align="center"|MP180
|align="center"|MP180
|align="center"|[[Image:Icon_coding.png]]<br> FliA2
|align="center"|[[Image:Icon_coding.png]]<br> FliA2
 +
|align="center"
|align="center"|mutated at EcoR1 and Pst1 internal site
|align="center"|mutated at EcoR1 and Pst1 internal site
|align="center"|[[Team:Paris/September 04|September 04]]
|align="center"|[[Team:Paris/September 04|September 04]]
Line 810: Line 786:
|align="center"|MP181
|align="center"|MP181
|align="center"|[[Image:Icon_coding.png]]<br> FliA3
|align="center"|[[Image:Icon_coding.png]]<br> FliA3
 +
|align="center"
|align="center"|FliA mutated at EcoR1 and Pst1 site
|align="center"|FliA mutated at EcoR1 and Pst1 site
|align="center"|[[Team:Paris/September 04|September 04]]
|align="center"|[[Team:Paris/September 04|September 04]]
Line 818: Line 795:
|align="center"|MP182
|align="center"|MP182
|align="center"|[[Image:Icon_coding.png]]<br> EnvZ*
|align="center"|[[Image:Icon_coding.png]]<br> EnvZ*
 +
|align="center"
|align="center"|the good one but not mutated for EcoR1 site
|align="center"|the good one but not mutated for EcoR1 site
|align="center"|[[Team:Paris/September 04|September 04]]
|align="center"|[[Team:Paris/September 04|September 04]]
Line 826: Line 804:
|align="center"|MP183
|align="center"|MP183
|align="center"|[[Image:Icon_coding.png]]<br> OmpR WT
|align="center"|[[Image:Icon_coding.png]]<br> OmpR WT
 +
|align="center"
|align="center"|OmpR WT not the mutated
|align="center"|OmpR WT not the mutated
|align="center"|[[Team:Paris/September 04|September 04]]
|align="center"|[[Team:Paris/September 04|September 04]]
Line 834: Line 813:
|align="center"|MP185
|align="center"|MP185
|align="center"|[[Image:Icon_coding.png]]<br> OmpR WT
|align="center"|[[Image:Icon_coding.png]]<br> OmpR WT
 +
|align="center"
|align="center"|OmpR WT not the mutated
|align="center"|OmpR WT not the mutated
|align="center"|[[Team:Paris/September 04|September 04]]
|align="center"|[[Team:Paris/September 04|September 04]]
Line 843: Line 823:
|align="center"|[[Image:Part_icon_reporter.png]]<br>YFP  
|align="center"|[[Image:Part_icon_reporter.png]]<br>YFP  
|
|
 +
|align="center"
|align="center"|[[Team:Paris/July 26|July 26]]
|align="center"|[[Team:Paris/July 26|July 26]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 851: Line 832:
|align="center"|[[Image:Part_icon_reporter.png]]<br>GFP
|align="center"|[[Image:Part_icon_reporter.png]]<br>GFP
|
|
 +
|align="center"
|align="center"|[[Team:Paris/July 23|July 23]], [[Team:Paris/July 26|July 26]]
|align="center"|[[Team:Paris/July 23|July 23]], [[Team:Paris/July 26|July 26]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 859: Line 841:
|align="center"|[[Image:Part_icon_reporter.png]]<br>mRFP
|align="center"|[[Image:Part_icon_reporter.png]]<br>mRFP
|
|
 +
|align="center"
|align="center"|[[Team:Paris/July 23|July 23]], [[Team:Paris/July 26|July 26]]
|align="center"|[[Team:Paris/July 23|July 23]], [[Team:Paris/July 26|July 26]]
Line 868: Line 851:
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>GFP generator
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>GFP generator
|align="center"|
|align="center"|
 +
|align="center"
|align="center"|[[Team:Paris/August 15|August 15]]
|align="center"|[[Team:Paris/August 15|August 15]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 876: Line 860:
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS+ ECFP LVA- term
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS+ ECFP LVA- term
|  
|  
 +
|align="center"
|align="center"|[[Team:Paris/August 20|August 20]]
|align="center"|[[Team:Paris/August 20|August 20]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 884: Line 869:
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS+ ECFP+ LVA+ term
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS+ ECFP+ LVA+ term
|
|
 +
|align="center"
|align="center"|[[Team:Paris/July 24|July 24]], [[Team:Paris/July 26|July 26]]<br>[[Team:Paris/August 20|August 20]]
|align="center"|[[Team:Paris/July 24|July 24]], [[Team:Paris/July 26|July 26]]<br>[[Team:Paris/August 20|August 20]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 892: Line 878:
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS+ YFP+ LVA- term
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS+ YFP+ LVA- term
|  
|  
 +
|align="center"
|align="center"|[[Team:Paris/August 20|August 20]]
|align="center"|[[Team:Paris/August 20|August 20]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 899: Line 886:
|align="center"|MP166
|align="center"|MP166
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS+ YFP LVA+ term   
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS+ YFP LVA+ term   
 +
|align="center"
|align="center"|BUT be careful , it doesn't work. See registry
|align="center"|BUT be careful , it doesn't work. See registry
|align="center"|[[Team:Paris/August 20|August 20]]
|align="center"|[[Team:Paris/August 20|August 20]]
Line 908: Line 896:
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>YFP tripart +AAV
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>YFP tripart +AAV
|
|
 +
|align="center"
|align="center"|[[Team:Paris/October 15|October 15]]
|align="center"|[[Team:Paris/October 15|October 15]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 916: Line 905:
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>gfp tri-part; strong rbs
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>gfp tri-part; strong rbs
|
|
 +
|align="center"
|align="center"|[[Team:Paris/July 26|July 26]]
|align="center"|[[Team:Paris/July 26|July 26]]
Line 925: Line 915:
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS+ mRFP LVA+ term
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS+ mRFP LVA+ term
|  
|  
 +
|align="center"
|align="center"|[[Team:Paris/August 20|August 20]]
|align="center"|[[Team:Paris/August 20|August 20]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 933: Line 924:
|align="center"|[[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Double terminator
|align="center"|[[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>Double terminator
|
|
 +
|align="center"
|align="center"|[[Team:Paris/July 24|July 24]], [[Team:Paris/July 26|July 26]]
|align="center"|[[Team:Paris/July 24|July 24]], [[Team:Paris/July 26|July 26]]
-
|- style="background: #649CD7;"
+
 
-
! colspan="8" style="background: #649CD7;" | Ligations: Constructions realised
+
 
-
|- style="background: #649CD7; text-align: center;"
+
-
|width=8%| Name/N°
+
-
|width=8%| Plasmid
+
-
|width=7%| Strain
+
-
|width=7%| Miniprep
+
-
|width=32%|Description
+
-
|width=20%| Digestion
+
-
|width=8%| comment
+
-
|width=10%| Date
+
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |L174
| style="background: #D4E2EF;" |L174
Line 999: Line 982:
|align="center"|OK sequencing
|align="center"|OK sequencing
|align="center"|[[Team:Paris/August 12|August 12]]
|align="center"|[[Team:Paris/August 12|August 12]]
-
|- style="background: #dddddd;" 
+
 
-
| style="background: #D4E2EF;" |L129
+
-
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
+
-
|align="center"|S144
+
-
|align="center"|MP145
+
-
|align="center"|[[Image:Part_icon_regulatory.png]]<br>pFlgB
+
-
|align="center"|D133 (FI) -  D136 (FV)
+
-
|align="center"|wrong sequencing
+
-
|align="center"|[[Team:Paris/August 12|August 12]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |L130
+
-
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
+
-
|align="center"|S145
+
-
|align="center"|MP146
+
-
|align="center"|[[Image:Part_icon_regulatory.png]]<br>pFlhB
+
-
|align="center"|D134 (FI) -  D136 (FV)
+
-
|align="center"|wrong sequencing 
+
-
|align="center"|[[Team:Paris/August 12|August 12]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |L131
+
-
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
+
-
|align="center"|
+
-
|align="center"|
+
-
|align="center"|[[Image:Part_icon_regulatory.png]]<br>pFlhDC
+
-
|align="center"|D136 (FV)- D135 (FI)
+
-
|align="center"|wrong sequencing
+
-
|align="center"|[[Team:Paris/August 8|August 8]]
+
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |L  
| style="background: #D4E2EF;" |L  
Line 1,091: Line 1,048:
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
-
 
-
|- style="background: #dddddd;" 
 
-
| style="background: #D4E2EF;" |L148
 
-
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
 
-
|align="center"| x
 
-
|align="center"| x
 
-
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>rbs (B0034)- OmpR*
 
-
|align="center"|D158 - D102
 
-
|align="center"|didn't work
 
-
|align="center"|[[Team:Paris/August 16|August 16 ]]
 
-
|- style="background: #dddddd;" 
 
-
| style="background: #D4E2EF;" |L149
 
-
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
 
-
|align="center"| x
 
-
|align="center"| x
 
-
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>rbs (B0034)- EnvZ
 
-
|align="center"|D159 - D102
 
-
|align="center"|didn't work
 
-
|align="center"|[[Team:Paris/August 16|August 16 ]]
 
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |L166
| style="background: #D4E2EF;" |L166
Line 1,175: Line 1,113:
|
|
|align="center"|[[Team:Paris/August 19|August 19]]
|align="center"|[[Team:Paris/August 19|August 19]]
-
|- style="background: #dddddd;" 
 
-
| style="background: #D4E2EF;" |L159
 
-
|align="center"|
 
-
|align="center"| x
 
-
|align="center"| x
 
-
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> rbs-lasI-dbl ter
 
-
|align="center"|D125 (FV) - D109 (FI)
 
-
|align="center"|didn't work
 
-
|align="center"|[[Team:Paris/August |August ]]
 
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |L138
| style="background: #D4E2EF;" |L138
Line 1,411: Line 1,340:
|align="center"|[[Team:Paris/August 13|August 13]]
|align="center"|[[Team:Paris/August 13|August 13]]
-
 
-
|- style="background: #dddddd;" 
 
-
| style="background: #D4E2EF;" |L163
 
-
|align="center"|[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]
 
-
|align="center"| x
 
-
|align="center"| x
 
-
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> rbs-lasI-gfp generator
 
-
|align="center"|D107 (BV) - D163 (BI)
 
-
|align="center"|
 
-
|align="center"|[[Team:Paris/August 22|August 22]]
 
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |L100
| style="background: #D4E2EF;" |L100
Line 1,439: Line 1,358:
|align="center"|DO NOT WORK !!!!
|align="center"|DO NOT WORK !!!!
|align="center"|[[Team:Paris/August 13|August 13]]
|align="center"|[[Team:Paris/August 13|August 13]]
-
|- style="background: #dddddd;" 
+
 
-
| style="background: #D4E2EF;" |L158
+
-
|align="center"|
+
-
|align="center"| x
+
-
|align="center"| x
+
-
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> rbs-tetR-gfp generator
+
-
|align="center"|D110(BV) - D131 (BI)
+
-
|align="center"|didn't work
+
-
|align="center"|[[Team:Paris/August 23|August 23]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |L162
+
-
|align="center"|[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]
+
-
|align="center"| x
+
-
|align="center"| x
+
-
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> rbs-TetR-gfp generator
+
-
|align="center"|D110 (BV) - D163 (BI)
+
-
|align="center"|
+
-
|align="center"|[[Team:Paris/August 22|August 22]]
+
-
|- style="background: #dddddd;" 
+
-
| style="background: #D4E2EF;" |L150
+
-
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
-
|align="center"| x
+
-
|align="center"|MP161
+
-
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas + rbs-tetR-gfp tripart
+
-
|align="center"|D105 (BV) - D146 (BI)
+
-
|incorrect clone <br>-> to do again
+
-
|align="center"|[[Team:Paris/August 19|August 19]]
+
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |L  
| style="background: #D4E2EF;" |L  
Line 1,475: Line 1,368:
|align="center"|in fact lack of pFlgA
|align="center"|in fact lack of pFlgA
|align="center"|[[Team:Paris/October 04|October 04]]
|align="center"|[[Team:Paris/October 04|October 04]]
-
 
-
|- style="background: #dddddd;" 
 
-
| style="background: #D4E2EF;" |L176
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|D125 (v) + D162 (i)
 
-
|
 
-
|align="center"|[[Team:Paris/September |September ]]
 
-
|- style="background: #dddddd;" 
 
-
| style="background: #D4E2EF;" |L180
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|D166 (v) + D109.2 (i)
 
-
|
 
-
|align="center"|[[Team:Paris/September |September ]]
 
-
|- style="background: #dddddd;" 
 
-
| style="background: #D4E2EF;" |L181
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|D199 (v) + D109.2 (i)
 
-
|
 
-
|align="center"|[[Team:Paris/September |September ]]
 
-
|- style="background: #dddddd;" 
 
-
| style="background: #D4E2EF;" |L182
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|D187 (v) + D109.2 (i)
 
-
|
 
-
|align="center"|[[Team:Paris/September |September ]]
 
-
 
-
|- style="background: #dddddd;" 
 
-
| style="background: #D4E2EF;" |L186
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|D179 (v) + D205 (i)
 
-
|
 
-
|align="center"|[[Team:Paris/September |September ]]
 
-
 
-
|- style="background: #dddddd;" 
 
-
| style="background: #D4E2EF;" |L190 ("L4")
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|D199 (v) + D (i)
 
-
|
 
-
|align="center"|[[Team:Paris/September |September ]]
 
-
 
-
|- style="background: #dddddd;" 
 
-
| style="background: #D4E2EF;" |L195 ("L9")
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|
 
-
|align="center"|D187 (v) + D194 (i)
 
-
|
 
-
|align="center"|[[Team:Paris/September |September ]]
 
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   

Revision as of 13:17, 26 October 2008

Contents

Last number used

Miniprep Strain Ligation Digestion PCR
216 216 234 202 154

(last checking on 19th october)

Freezer

PCR products

PCR products
Number Name Oligo F Oligo R Template Length Comments Product Left ? [DNA]µg/mL Date
PCR124 pFlgA O100 O101 - 261pb yes 0.5 August 7
PCR125 pFlgB O102 O103 - 261pb yes 0.5 August 7
PCR126 pFlhB O108 O109 - 260pb yes 0.5 August 7
PCR127 FlhDC O132 O131 MG1655 no August 8
PCR128 OmpR* O138 O139 Strain OmpR* 700pb yes 150 August 8
PCR129 EnvZ* O126 O127 Strain EnvZ* 1400pb yes 100 August 8
PCR130 E0240 rbs+ O141 O140 MP143 900pb no August 11
PCR131 FlhD rbs- O132 O133 MG1655 350pb no August 11
PCR132 pFlhD rbs- O130 O131 MG1655 600pb no August 11
PCR133 FlhDC + prom O110 O131 MG1655 1300pb no August 11
PCR134 FlhDC + prom O111 O131 MG1655 no August 11
PCR135 pFliL O124 O125 MG1655 yes August 11
PCR136 pFlhDC O110 O113 MG1655 yes August 11
PCR137 pFlhDC O 111 O 113 MG1655 yes August 11
PCR138 FlhDC (RBS -) O 132 O 131 MG 1655 yes August 15
PCR139 pFlhDC (URI) O 110 O 113 PCR 130 yes August 15
PCR140 FlhDC (RBS -) O 132 O 131 PCR 130 yes August 15
PCR141 Gene FlhDC + rbs nat O 134 O 131 MG1655 1000pb yes August 26
PCR142 pFliA + rbs nat O 145 O 144 MG1655 325pb yes August 26
PCR143 pFliA sans rbs O 145 O 146 MG1655 310pb yes August 26
PCR144 pFliA + Gene FliA O 145 O 151 MG1655 1100pb yes August 26
PCR145 FliA1 O 148 O 152 MG1655 150pb yes August 26
PCR146 FliA2 O 153 O 150 MG1655 670pb yes August 26
PCR 147 EnvZ* O 126 O 127 S 120 1412 bp yes 150 August 26
PCR 148 OmpR* O 138 O 139 S 119 789 bp yes 101 August 26
PCR 149 pFlhDC (Soutourina +RBS) O 154 O 113 MG1655 450 bp yes August 26
PCR 151 pFLiA without nat. rbs O 145 O 146 MG1655 310 bp yes 34 August 26
PCR 152 pFliA rbs nat. O 145 O 144 MG1655 325 bp yes 19 August 26
PCR 153 pFliA+Gene FliA non-mutated O 145 O 151 MG1655 1100 bp yes 39 August 26
PCR 154 Gene FliA mutated (EcoRI/PstI) O 148 O 150 MG1655 800 bp 154.1, 154.2, 154.3 yes 50 August 26

Biobricks

Ligations: Constructions realised
Name/N° Plasmid Strain Miniprep Description Digestion comment Date
EnvZ* I281S Strain S120 Strain MG1655
Icon coding.png
EnvZ* 		July 23
OmpR* M57I Strain S119 Strain MG1655
Icon coding.png
OmpR* 		Really mutated? July 23
MG1655 Strain S141 Strain Aug 7
NEC283 Strain S226 Strain MG1655 Δ FliA Frt October 25
NEC284 Strain S227 Strain MG1655 Δ FlhD Frt October 25
NEC285 Strain S228 Strain MG1655 Δ FlgM Frt October 25
Δ EnvZ :: Cm Strain S229 Strain MG1655 Δ EnvZ :: Cm October 25
[http://partsregistry.org/Part:BBa_J61002 J61002] [http://partsregistry.org/Part:BBa_J61002 J61002] S142 MP123 Icon plasmid.png
plasmid 		August 5
[http://partsregistry.org/Part:pSB1A3 pSB1A3] [http://partsregistry.org/Part:pSB1A3 pSB1A3] S122 MP115 Icon plasmid.png
plasmid 		July 23
[http://partsregistry.org/Part:pSB4T5 pSB4T5] [http://partsregistry.org/Part:pSB4T5 pSB4T5] S215 MP215 Icon plasmid.png
plasmid 		low copy plasmid with pSC101 ORI October 14
[http://partsregistry.org/Part:pSB3K3 pSB3K3] [http://partsregistry.org/Part:pSB3K3 pSB3K3] S158 MP142 Icon plasmid.png
plasmid 		low to medium copy plasmid with p15A ORI August 15
U317 pU317 S225 MP225 Icon plasmid.png
plasmid 		FliA in pBad18 (ORI pBR / ampicillin resistance) October 23
JM45 pJM45 S217 MP217 Icon plasmid.png
plasmid 		FlhDC in pBad18 (ORI pBR / ampicillin resistance) October 24
[http://partsregistry.org/Part:BBa_J23100 J23100] [http://partsregistry.org/Part:BBa_J61002 J61002] x MP116 Part icon regulatory.png
Strong constitutive promoter 		July 26
[http://partsregistry.org/Part:BBa_J23101 J23101] [http://partsregistry.org/Part:BBa_J61002 J61002 ] S109 MP101 Part icon regulatory.png
Strongest Constitutive promoter 		July 22, July 23, July 26
[http://partsregistry.org/Part:BBa_J23107 J23107] [http://partsregistry.org/Part:BBa_J61002 J61002] x MP117 Part icon regulatory.png
Medium constitutive promoter 		July 24, July 26
[http://partsregistry.org/Part:BBa_J23109 J23109] [http://partsregistry.org/Part:BBa_J61002 J61002] S110 MP102 Part icon regulatory.png
Weak Constitutive promoter 		July 22, July 26
[http://partsregistry.org/Part:BBa_I0500 I0500] [http://partsregistry.org/Part:pSB2K3 pSB2K3] S103 MP119 Part icon regulatory.png
AraC pBAD 		July 24, July 26
[http://partsregistry.org/Part:BBa_R0040 R0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S111 MP104 Part icon regulatory.png
tetR repressible promoter 		July 23, July 26
[http://partsregistry.org/Part:BBa_R0079 R0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S108 MP103 Part icon regulatory.png
promoter pLas (lasr & PAI regulated) 		July 23, July 26
L [http://partsregistry.org/Part:J61002 J61002] S207 MP207 Icon regulatory.png
pFliA 		October 04
L [http://partsregistry.org/Part:pSB3K3 pSB3K3] S211 MP211 Icon regulatory.pngIcon rbs.png
pFlgA(RBS WT) 		October 10
pFlgB pCR4Blunt-TOPO S187 MP184 Icon regulatory.pngIcon rbs.png
pFlgB-RBS (WT) 		September 04
pFlhB pCR4Blunt-TOPO S178 MP175 Icon regulatory.pngIcon rbs.png
pFlhB-RBS (WT) 		September 04
L [http://partsregistry.org/Part:pSB3K3 pSB3K3] S212 MP212 Icon regulatory.pngIcon rbs.png
pFlhB(RBS WT) 		October 10
L pCR4Blunt-TOPO S180 MP177 Icon regulatory.pngIcon rbs.png
pFlhDC-RBS (WT) 		September 04
L pCR4Blunt-TOPO S179 MP176 Icon regulatory.pngIcon rbs.png
pFliA-RBS (WT) 		pFliA with conserved Pst1 site September 04
L pCR4Blunt-TOPO S181 MP178 Icon regulatory.pngIcon rbs.png
pFliL-RBS (WT) 		September 04
L [http://partsregistry.org/Part:pSB3K3 pSB3K3] S210 MP210 Icon regulatory.pngIcon rbs.png
pFliL(RBS WT) 		October 10
[http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] x MP120 Part icon rbs.png
Strong RBS (Efficiency = 0,6) 		July 26
[http://partsregistry.org/Part:BBa_B0032 B0032] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S163 MP163 Icon rbs.png
medium rbs (efficiency = 0,3) 		August 20
[http://partsregistry.org/Part:BBa_B0034 B0034 ] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S107 MP100 Part icon rbs.png
Strongest RBS (Efficiency = 1) 		July 26
[http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S112 MP105 Part icon rbs.pngIcon coding.png
B0034(rbs) ->LasI 		July 26
[http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S113 MP106 Part icon rbs.pngIcon coding.png
B0034(rbs) -> TetR 		August 24
L pCR4Blunt-TOPO S182 MP179 Part icon rbs.pngIcon coding.png
RBS(FlhDC) - FlhDC 		FlhDC with conserved Pst1 site September 04
[http://partsregistry.org/Part:BBa_C0040 C0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S121 MP114 Icon coding.png
tetR repressor 		July 23
[http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] S114 MP107 Icon coding.png
lasR activator with LVA Tag 		July 23
[http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S115 MP108 Icon coding.png
lasR activator 		July 22, July 23, July 26


L132 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S153 MP154 Icon coding.png
flhDC 		D142(FV) - D139(FI) August 13
L133 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S154 MP155 Icon coding.png
OmpR* 		D142(FV) - D140(FI) WT sequence August 19
L134 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S155 MP156 Icon coding.png
EnvZ* 		D142(FV) - D141(FI) align="center"lacks about 300 bp in EnvZ* August 19
L140 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png
flhD 		D145 (V) - D141 (I) align="center" August 12
L141 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S160 MP159 Icon coding.png
flhC 		D142 (ES) - D145 (ES) align="center"has not been sequenced August 15
L pCR4Blunt-TOPO S183 MP180 Icon coding.png
FliA2 		align="center" mutated at EcoR1 and Pst1 internal site September 04
L pCR4Blunt-TOPO S184 MP181 Icon coding.png
FliA3 		align="center" FliA mutated at EcoR1 and Pst1 site September 04
L pCR4Blunt-TOPO S185 MP182 Icon coding.png
EnvZ* 		align="center" the good one but not mutated for EcoR1 site September 04
L pCR4Blunt-TOPO S186 MP183 Icon coding.png
OmpR WT 		align="center" OmpR WT not the mutated September 04
L pCR4Blunt-TOPO S188 MP185 Icon coding.png
OmpR WT 		align="center" OmpR WT not the mutated September 04
[http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] S116 MP109 Part icon reporter.png
YFP 		align="center" July 26
[http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S117 MP110 Part icon reporter.png
GFP 		align="center" July 23, July 26
[http://partsregistry.org/Part:BBa_E1010 E1010 ] [http://partsregistry.org/Part:pSB2K3 pSB2K3] S118 MP111 Part icon reporter.png
mRFP 		align="center" July 23, July 26
[http://partsregistry.org/Part:BBa_E0240 E0240] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S157 MP143 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
GFP generator 		align="center" August 15
[http://partsregistry.org/Part:BBa_E0420 E0420] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S167 MP167 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS+ ECFP LVA- term 		align="center" August 20
[http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S164 MP121
MP164 		Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS+ ECFP+ LVA+ term 		align="center" July 24, July 26
August 20
[http://partsregistry.org/Part:BBa_E0430 E0430] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S165 MP165 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS+ YFP+ LVA- term 		align="center" August 20
[http://partsregistry.org/Part:BBa_E0432 E0432] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S166 MP166 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS+ YFP LVA+ term 		align="center" BUT be careful , it doesn't work. See registry August 20
[http://partsregistry.org/Part:BBa_E0434 E0434] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] S216 MP216 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
YFP tripart +AAV 		align="center" October 15
[http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S106 MP122 Part icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
gfp tri-part; strong rbs 		align="center" July 26
[http://partsregistry.org/Part:BBa_I732078 I732078] [http://partsregistry.org/Part:pSB1A3 pSB1A3] S168 MP168 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS+ mRFP LVA+ term 		align="center" August 20
[http://partsregistry.org/Part:BBa_B0015 B0015] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] x MP118 Part icon terminator.pngPart icon terminator.png
Double terminator 		align="center" July 24, July 26


L174 [http://partsregistry.org/Part:J61002 J61002] S MP202
Icon regulatory.pngIcon coding.png
Strong constitutive promoter-GFP generator 		J23101-E0240 September 26
L142 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S161 MP160 Icon regulatory.pngIcon rbs.pngIcon coding.png
flhDC+promotor 		D143 (ES) - D145 (ES) does not have
the insert 		August 15
L179 [http://partsregistry.org/Part:J61002 J61002] S200 MP200 Icon regulatory.pngIcon rbs.pngIcon coding.png
J23101 + RBS (B0032) + tet R 		September 24
L [http://partsregistry.org/Part:J61002 J61002] S201 MP201 Icon regulatory.pngIcon rbs.pngIcon coding.png
pFlgA(RBS WT)-EnvZ* 		probably wrong according to MP208 and MP209 that have been sequenced September 24
L [http://partsregistry.org/Part:pSB3K3 pSB3K3] S208 MP208 Icon regulatory.pngIcon rbs.pngIcon coding.png
pFlgA(RBS WT)-EnvZ* 		in fact lack of pFlgA October 04
L128 [http://partsregistry.org/Part:J61002 J61002] S143 MP144 Part icon regulatory.png
pFlgA 		D132 (FI) - D136 (FV) OK sequencing August 12
L [http://partsregistry.org/Part:BBa_J61002 J61002] S213 MP213 Icon regulatory.pngIcon rbs.png
pFlhB(RBS WT) 		October 10
L [http://partsregistry.org/Part:BBa_J61002 J61002] S214 MP214 Icon regulatory.pngIcon rbs.png
pFliL(RBS WT) 		October 10
L102 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S123 MP124 Part icon rbs.pngPart icon reporter.png
Strong rbs - YFP 		D129 (BV) - D118 (BI) August 1
L103 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S124 MP125 Part icon rbs.pngPart icon reporter.png
Strong rbs - mRFP 		D129 (BV) - D122 (BI) August 1
L124 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S139 MP140 Part icon rbs.pngPart icon reporter.png
Strongest RBS - rfp 		D102 (BV) - D122 (BI) August 1
L125 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S140 MP141 Part icon rbs.pngPart icon reporter.png
Strongest RBS - yfp 		D102 (BV) - D118 (BI) August 1
L104 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S125 MP126 Part icon rbs.pngIcon coding.png
Strong rbs - lasR activator 		D129 (BV) - D114 (BI) August 1
L166 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S173 MP172 Part icon rbs.pngIcon coding.png
RBS(B0032) - tetR 		August 25
L126 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S152 MP153 Part icon rbs.pngIcon coding.png
Strongest RBS (1)- LasR activator (+LVA) 		D102 (BV) - D114 (BI) August 13
L157 Icon coding.pngIcon rbs.png
tetR-rbs (B0015) 		D125 (BV) - D162 (BI) August 23
L117 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] S135 MP136 Part icon reporter.pngPart icon terminator.pngPart icon terminator.png
yfp - Double Terminator 		D125 (FV) - D117 (FI) August 1
L118 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] S136 MP137 Part icon reporter.pngPart icon terminator.pngPart icon terminator.png
rfp - Double Terminator 		D125 (FV) - D121 (FI) August 1
L119 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] S137 MP138 Icon coding.pngPart icon terminator.pngPart icon terminator.png
lasR activator + LVA - Double Terminator 		D125 (FV) - D113 (FI) August 1


L151 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] S162 MP162 Icon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png
rbs-lasR + dbl ter 		D147 (FI) - D125 (FV) August 19
L138 [http://partsregistry.org/Part:pSB3K3 pSB3K3] S156 MP157 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
gfp generator (E0240) 		D137(FV) - D138(FI) August 13
L139 [http://partsregistry.org/Part:pSB3K3 pSB3K3] S159 MP158 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
E0240 RBS+ 		D140 (PstI) - D144 (ES) August 15
L167 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S172 MP171 Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.pngPart icon regulatory.png
gfp generator(E0240) - pTet 		August 25
L153 [http://partsregistry.org/Part:BBa_J61002 J61002] S169 MP169 Icon regulatory.pngIcon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png
Strong promoter-rbs-lasR-dble ter 		D123 (BV) - D165 (BI) August 20
L154 [http://partsregistry.org/Part:BBa_J61002 J61002] S170 MP170 Icon regulatory.pngIcon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png
strongest promoter-rbs-lasR-dble ter 		D103 (BV) - D165 (BI) August 20
L111 [http://partsregistry.org/Part:J61002 J61002] S131 MP132 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
Weak promoter - ECFP 		D104 (BV) - D130 (BI) August 1
L105 [http://partsregistry.org/Part:J61002 J61002] S126 MP127 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
Strong promoter - ECFP 		D123 (BV) - D130 (BI) August 1
L107 [http://partsregistry.org/Part:J61002 J61002] S128 MP129 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
Strongest promoter - ECFP 		D103 (BV) - D130 (BI) August 1
L112 [http://partsregistry.org/Part:J61002 J61002] S132 MP133 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
Weak promoter - GFP tripart 		D104 (BV) - D131 (BI) August 1
L110 [http://partsregistry.org/Part:J61002 J61002] S130 MP131 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
Medium promoter - gfp Tripart 		D124 (BV) - D131 (BI) August 1
L106 [http://partsregistry.org/Part:J61002 J61002] S127 MP128 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
Strong promoter - gfp Tripart 		D123 (BV) - D131 (BI) August 1
L108 [http://partsregistry.org/Part:J61002 J61002] S129 MP130 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
Strongest promoter - gfp Tripart 		D103 (BV) - D131 (BI) August 1
L155 Icon regulatory.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
strongest promoter-gfp generator 		D164(FV) - D163 (FI) August 22
L114 [http://partsregistry.org/Part:pSB2K3 pSB2K3] S148 MP149 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
AracpBAD - gfp tripart 		D126 (BV) - D131 (BI) August 13
L160 [http://partsregistry.org/Part:BBa_J61002 J61002] S174 MP173 Icon regulatory.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA - YFP Tripart (LVA-) 		D166 (FV) - D132 (FI) Fluorescence OK August 22
L161 [http://partsregistry.org/Part:BBa_J61002 J61002] S175 MP174 Icon regulatory.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA - YFP Tripart (LVA+) 		D167 (FV) - D132 (FI) August 22
L115 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S133 MP134 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pLas - ECFP 		D105 (BV) - D130 (BI) August 1
L120 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S149 MP150 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pTet - ECFP 		D106 (BV) - D130 (BI) August 13
L116 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S134 MP135 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pLas - gfp Tripart 		D105 (BV) - D131 (BI) August 1
L121 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S138 MP139 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pTet - gfp tripart 		D106 (BV) - D131 (BI)
In Top10 strain 		August 1
L121 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S171 not done Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pTet - gfp Tripart 		D106 (BV) - D131 (BI) Same as S138 but in chemically labmade competent MG1655 bacteria. For study of doubling time of population. Aug 22
L156 Icon regulatory.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pTet-gfp generator 		D161 (FV) - D163 (FI) August 22




L122 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S150 MP151 Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS-lasI - ECFP 		D107 (BV) - D130 (BI) August 13
L123 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S151 MP152 Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS lasI - gfp tripart 		D107 (BV) - D131 (BI) August 13
L100 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S146 MP147 Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
rbs TetR - ECFP 		D110 (BV) - D130 (BI) August 13
L101 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S147 MP148 Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
rbs TetR - GFP tripart 		D110 (BV) - D131 (BI) DO NOT WORK !!!! August 13
L [http://partsregistry.org/Part:J61002 J61002] S209 MP209 Icon regulatory.pngIcon rbs.pngIcon coding.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA(RBS WT)-EnvZ*-mRFP tripart LVA+ 		in fact lack of pFlgA October 04
L226 [http://partsregistry.org/Part:J61002 J61002] S218 MP218 Icon regulatory.pngIcon rbs.pngIcon coding.png
pFlhB(RBS WT)-EnvZ* 		October 24
L227 [http://partsregistry.org/Part:J61002 J61002] S219 MP219 Icon regulatory.pngIcon rbs.pngIcon reporter.png
pFliL(RBS WT)-GFP 		October 24
L228 [http://partsregistry.org/Part:J61002 J61002] S220 MP220 Icon regulatory.pngIcon rbs.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFliL(RBS WT)-ECFP tripart (+LVA) 		October 24
L229 [http://partsregistry.org/Part:J61002 J61002] S221 MP221 Icon regulatory.pngIcon rbs.pngIcon reporter.png
pFlgA(RBS WT)-GFP 		October 24
L232 [http://partsregistry.org/Part:J61002 J61002] S222 MP222 Icon regulatory.pngIcon rbs.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA(RBS WT)-YFP tripart (+AAV) 		October 24
L234 [http://partsregistry.org/Part:J61002 J61002] S223 MP223 Icon regulatory.pngIcon rbs.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlhB(RBS WT)-mRFP tripart (+LVA) 		October 24

rebut

Ligations: Constructions realised
Name/N° Plasmid Strain Miniprep Description Digestion comment Date
L135 [http://partsregistry.org/Part:pSB3K3 pSB3K3] Part icon regulatory.png
pFlgA 		D137 (BV) - D133 (BI)
don't work 		August 9
L136 [http://partsregistry.org/Part:pSB3K3 pSB3K3] Part icon regulatory.png
pFlgB 		D137 (BV) - D1343 (BI)
don't work 		August 9
L137 [http://partsregistry.org/Part:pSB3K3 pSB3K3] Part icon regulatory.png
pFlhB 		D137 (BV) - D135 (BI)
don't work 		August 9
L147 [http://partsregistry.org/Part:J61002 J61002] x x Icon regulatory.png
pFlhDC 		D155 - D136
didn't work 		August 16
L143 [http://partsregistry.org/Part:pSB3K3 pSB3K3] x x Icon regulatory.png
pFliL 		D149 - D137
didn't work 		August 14
L144 [http://partsregistry.org/Part:pSB3K3 pSB3K3] x x Icon regulatory.png
pFliL 		D150 - D152
didn't work 		August 14
L145 [http://partsregistry.org/Part:pSB1A2 pSB1A2] x x Icon coding.png
FlhDC 		D1543 - D145
didn't work 		August 16
L146 [http://partsregistry.org/Part:pSB1A2 pSB1A2] x x Icon coding.png
FlhDC 		D154 - D145
didn't work 		August 16
L129 [http://partsregistry.org/Part:J61002 J61002] S144 MP145 Part icon regulatory.png
pFlgB 		D133 (FI) - D136 (FV) wrong sequencing August 12
L130 [http://partsregistry.org/Part:J61002 J61002] S145 MP146 Part icon regulatory.png
pFlhB 		D134 (FI) - D136 (FV) wrong sequencing August 12
L131 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.png
pFlhDC 		D136 (FV)- D135 (FI) wrong sequencing August 8
L148 [http://partsregistry.org/Part:pSB1A2 pSB1A2] x x Icon rbs.pngIcon coding.png
rbs (B0034)- OmpR* 		D158 - D102 didn't work August 16
L149 [http://partsregistry.org/Part:pSB1A2 pSB1A2] x x Icon rbs.pngIcon coding.png
rbs (B0034)- EnvZ 		D159 - D102 didn't work August 16
L159 x x Icon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png
rbs-lasI-dbl ter 		D125 (FV) - D109 (FI) didn't work August
L163 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] x x Icon rbs.pngIcon coding.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
rbs-lasI-gfp generator 		D107 (BV) - D163 (BI) August 22
L158 x x Icon rbs.pngIcon coding.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
rbs-tetR-gfp generator 		D110(BV) - D131 (BI) didn't work August 23
L162 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] x x Icon rbs.pngIcon coding.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
rbs-TetR-gfp generator 		D110 (BV) - D163 (BI) August 22
L150 [http://partsregistry.org/Part:pSB1A2 pSB1A2] x MP161 Icon regulatory.pngIcon rbs.pngIcon coding.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pLas + rbs-tetR-gfp tripart 		D105 (BV) - D146 (BI) incorrect clone
-> to do again 		August 19
L176 D125 (v) + D162 (i) September
L180 D166 (v) + D109.2 (i) September
L181 D199 (v) + D109.2 (i) September
L182 D187 (v) + D109.2 (i) September
L186 D179 (v) + D205 (i) September
L190 ("L4") D199 (v) + D (i) September
L195 ("L9") D187 (v) + D194 (i) September

Digestion Products

(Legend: Vector, Insert) Ø

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Obs Size Matrix (bp) Size BB (bp) Description Product in use []ng/µL Date
D100.1 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 34 10 July 26
D100.2
D101 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2076 15 10 July 29
D102.1 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2077 14 10 July 26
August 15
D102.2
D103.1 [http://partsregistry.org/Part:BBa_J23101 J23101] [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV 2100 883 27 July 26
D103.2 35
D104.1 [http://partsregistry.org/Part:BBa_J23109 J23109] [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV 2100 883 20 July 26
D104.2
D105.1 [http://partsregistry.org/Part:BBa_R0079 R0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2222 14 5 July 26
D105.2
D106.1 [http://partsregistry.org/Part:BBa_R0040 R0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2119 14 8 July 26
D106.2
D107 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2750 14 August 23 5
12 		July 26
July 23
D108.1 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 707 5 July 29
D108.2
D109 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2056 708 July 26 10 July 26
D110 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2768 14 August 23 20
8
19 		July 26
August 23
August 28
D111.1 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 725 20 July 26
D111.2
D112 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2079 703 8 September 5
D113 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] EcoRI SpeI FI 4402 779 20 July 26
D114 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] XbaI PstI BI 4003 778 20 July 26
D115.1 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 4402 746 - July 29
D115.2
D116.1 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 745 20 July 29
D116.1
D117 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI SpeI FI 3166 746 5 July 29
D118 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB2K3 pSB2K3] XbaI PstI BI 3167 745 20 July 26
D119 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2056 743 10 July 26
D120 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 742 10 July 26
D121 [http://partsregistry.org/Part:BBa_E1010 E1010] [http://partsregistry.org/Part:pSB2K3 pSB2K3] EcoRI SpeI FI 4402 704 10 July 26
D122 [http://partsregistry.org/Part:BBa_E1010 E1010] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 703 10 July 26
D123.1 [http://partsregistry.org/Part:BBa_J23100 J23100] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 26 July 26
D123.2 23
D124.1 [http://partsregistry.org/Part:BBa_J23107 J23107] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 15 July 26
D124.2
D125.1 [http://partsregistry.org/Part:BBa_B0015 B0015] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI XbaI FV 3303 15 26
10 		July 26
August 23
August 28
D125.2 24
3687
D126.1 [http://partsregistry.org/Part:BBa_I0500 I0500] [http://partsregistry.org/Part:pSB2K3 pSB2K3] SpeI PstI BV 5621 14 10 July 26
D126.2
D127.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 37 10 July 26
D127.2
D128.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2079 15 10 July 29
D128.1
D129.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2080 14 10 July 29
D129.2
D130.1 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 939 10 July 26
D130.2
D130.3
D131.1 [http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 900 8 July 26
D131.2
D131.3
D132 pFlgA - EcoRI SpeI FI - 250 5 August 6
D133 pFlgB - EcoRI SpeI FI - 250 5 August 6
D134 pFlhB - EcoRI SpeI FI - 249 5 August 6
D135 pFlhDC - EcoRI SpeI FI - problem August 6
D136 - [http://partsregistry.org/Part:BBa_J61002 J61002] EcoRI SpeI FV 2925 - August 6
August 16
D137 pSB3K3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] EcoRI SpeI FV 2727 - August 6
D138 E0240 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 899 - August 6
D139 flhDC - EcoRI PstI FI - 972 August 6
D140 E040 rbs+ - PstI PstI I - August 12
D141 flhD RBS - - EcoRI SpeI FI - August 12
D142 flhC RBS - - EcoRI SpeI FI - August 12
D143 flhDC + promoter - EcoRI SpeI FI - August 12
D144 pSB3K3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] EcoRI SpeI FI - August 12
D145 pSB1A2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI - August 12
August 15
D146 MP148 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI 2057 1609 rbs TetR - GFP tripart
D110 (BV) - D131 (BI)
Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png 		August 15
D147 MP153 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 2056 799 Strongest RBS (1)- LasR activator (+LVA)
D102 (BV) - D114 (BI)
Part icon rbs.pngIcon coding.png 		August 15
D149 pfliL - EcoRI SpeI FI - August 14
August 16
D150 pfliL - XbaI PstI BI - August 14
August 16
D152 pSB3K3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] XbaI PstI BI - August 14
D153 PCR 138 - gène flhDC - EcoRI SpeI FI - August 15
D154 PCR 140 - gène flhDC - EcoRI SpeI FI - August 15
D155 PCR 139 - pflhDC (URI) - EcoRI SpeI FI - August 15
D158 MP 155.1 - OmpR* [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI - August 15
D159 MP 156.1 - EnvZ* [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI - August 15
D161 MP 104 - pTet [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV - 39 August 16
D162 MP 114 - TetR [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI - 11 August 16
D163 MP 143 - gfp generator [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI - August 23 14
11 		August 16
August 23
D164 MP 101 - pJ23101 [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV - 3 August 16
D165 MP162.2 [http://partsregistry.org/Part:BBa_pSB1AK3 pSB1AK3] XbaI PstI BI 2057 798 rbs-lasR - double terminator
D147 (FI) - D125 (FV)
Part icon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png 		27 August 18
D166 MP 165.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV - 2942 RBS (B0034)+ YFP+ LVA- term B0015
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
59 		August 26
September 3
D179 MP3.4 or MP118 [http://partsregistry.org/Part:BBa_pSB1AK3 pSB1AK3] SpeI PstI BV B0015 (double terminator B0010-B0012)
Part icon terminator.pngPart icon terminator.png 		August 20
D180 MP101.1 [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV promoter J23101
Part icon regulatory.png 		August 20
D181 MP104.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV pTet (TetR repressible promoter)
Part icon regulatory.png 		August 20
D182 MP114.1 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI TetR
Icon coding.png 		August 20
D183 MP119.3 - XbaI PstI BI pBad promoter
Part icon regulatory.png 		August 20
D184 MP143.1 [http://partsregistry.org/Part:BBa_pSB2K3 pSB2K3] EcoRI SpeI FI gfp generator
Part icon reporter.png 		August 18
D185 MP163.1 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV B0032 RBS
Part icon rbs.png 		August 18
D187 MP168.1 [http://partsregistry.org/Part:BBa_pSB1A3 pSB1A3] EcoRI XbaI FV RBS (B0034)+ mRFP LVA+ term B0015
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
43
September 3
D188 PCR 138 - XbaI PstI Insert - 767 OmpR*
Icon coding.png 		August 26
D189 MP 101.2 [http://partsregistry.org/Part:BBa_J61002 J61002] XbaI PstI Vector - 2073 pSB1A2
Icon plasmid.png 		August 26
D191 MP114.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV 2724 tetR
Icon coding.png 		22 August 29
D192 MP144.2 [http://partsregistry.org/Part:BBa_J61002 J61002] EcoRI XbaI FV 2267 FlgA
Part icon regulatory.png 		26 August 29
D193 MP171 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 961 gfp generator(E0240) - pTet
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.pngPart icon regulatory.png 		9,5 August 29
D194 MP172.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 704 RBS(B0032) - tetR
Part icon rbs.pngIcon coding.png 		13 August 29
D195 MP172.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI 703 RBS(B0032) - tetR
Part icon rbs.pngIcon coding.png 		11 August 29
D196 MP104.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV 2119 pTet
Part icon regulatory.png 		45 September 3
D197 MP157.2 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] XbaI PstI BI 898 gfp generator(E0240)
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png 		12 September 3
D198 MP164.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV 2981 RBS (B0034)+ ECFP+ LVA+ term B0015
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png 		36 September 3
D199 MP167.1 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV 2942 RBS (B0034)+ ECFP LVA- term (B0015)
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png 		61 September 3
D200 MP171.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI 960 gfp generator(E0240) - pTet
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.pngPart icon regulatory.png 		43 September 3
D201 MP114.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV 2724 tetR
Icon coding.png 		13 August 29
D202 MP101.1 [http://partsregistry.org/Part:BBa_J61002 J61002] EcoRI nothing 2984 25 September 8
D203 MP101.2 [http://partsregistry.org/Part:BBa_J61002 J61002] BspHI nothing 1870
1008
105 		September 11
D204 MP144 [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI September 22
D205 MP101 [http://partsregistry.org/Part:BBa_J61002 J61002] XbaI PstI September 22
D206 MP179 XbaI PstI September 22
D207 MP182.3 XbaI PstI September
D208 MP175 EcoRI SpeI September
D209 MP144 [http://partsregistry.org/Part:BBa_J61002 J61002] EcoRI SpeI September
D210 MP178 EcoRI SpeI September
Retrieved from "http://2008.igem.org/Team:Paris/Notebook/Freezer"