"
Team:Heidelberg/Notebook/Sensing Group/Notebook/6thweek
From 2008.igem.org
(Difference between revisions)
(→Monday, 09/08/2008) |
(→Tuesdsay, 09/09/2008) |
||
| Line 135: | Line 135: | ||
== Tuesdsay, 09/09/2008 == | == Tuesdsay, 09/09/2008 == | ||
| + | * Miniprep of LuxQ colonies no. 1,2,3,5,6 | ||
| + | [[Image:HD 080909-PCR Miniprep-LuxQ 2.png|left|thumb|150px]][[Image:HD 080909-PCR Miniprep-LuxQ 1.png|left|thumb|150px]] | ||
== Wednesday, 09/10/2008 == | == Wednesday, 09/10/2008 == | ||
Revision as of 17:02, 26 October 2008
Contents |
Monday, 09/08/2008
- Liquid Culture of 5 colonies from LuxQ Transformation and 1 from F1 transformation
- Miniprep of Liquid Culture from previous Transformation (done by Chenchen). Incubated for two days @ RT and about 6 h @ 37 °C
- Digestion of the Miniprep products with xbaI (Buffer2 + BSA, 1 ul enzyme @ 37 °C about 40min)
Sequencing @ GATC yielded correct sequence for LuxQ no. 1
- PCR for LuxQ and LuxP with Phusion (25 µl Mastermix, 0.5 µl each Primer, 0(1) µl DMSO, 24(23) µl H20, 0.5 µl Template)
- PCR Programme: 5 min @ 98 °C || 10 s @ 98 °C | 30 s @ 55 °C | 30 s @ 72 °C || 5 min @ 72 °C | 4 °C
Tuesdsay, 09/09/2008
- Miniprep of LuxQ colonies no. 1,2,3,5,6
