Brown: Team Resistance/26 October 2008

From 2008.igem.org

(Difference between revisions)
(New page: {{BNBK}} ===26 October 2008=== *Made new culture of the PVJ4 transformation so that a miniprep can be done. *New culture of gene S105 part made. *Will run a gel of the S105 gene restri...)
(→26 October 2008)
Line 5: Line 5:
*New culture of gene S105 part made.
*New culture of gene S105 part made.
-
*Will run a gel of the S105 gene restriction digest with EcoRI and SpeI for verification of part.
+
*Will run a gel of the S105 gene restriction digest with EcoRI and SpeI for verification of part. The gel showed a faint band at the length we hoped for but there was no other band verifying the vector.  We should have seen two bands but we only saw one.
 +
 
 +
 
 +
====Conductivity Tests====
 +
 
 +
*PVJ4 Colony 2 and 4 plus Controls.  10ml cultures.  420 ul of 50% Arabinose solution will be added.  Conductance measurements will be taken before and after lysis has occurred. 
 +
*Throughout the test, Optical Density measurements will be taken.

Revision as of 20:05, 26 October 2008


Toxipop lab notebook.png

26 October 2008

  • Made new culture of the PVJ4 transformation so that a miniprep can be done.
  • New culture of gene S105 part made.
  • Will run a gel of the S105 gene restriction digest with EcoRI and SpeI for verification of part. The gel showed a faint band at the length we hoped for but there was no other band verifying the vector. We should have seen two bands but we only saw one.


Conductivity Tests

  • PVJ4 Colony 2 and 4 plus Controls. 10ml cultures. 420 ul of 50% Arabinose solution will be added. Conductance measurements will be taken before and after lysis has occurred.
  • Throughout the test, Optical Density measurements will be taken.
Retrieved from "http://2008.igem.org/Brown:_Team_Resistance/26_October_2008"