Team:Chiba/Calendar-Home/6 September 2008

From 2008.igem.org

(Difference between revisions)

Revision as of 22:07, 29 October 2008

>Home | Notebook

5 September 2008 <|> 7 September 2008

Meeting with UmeG

the ppt (in Japanese) for our weekly meeting

File:Chiba-0809060855.ppt

Laboratory work

Team:Input

no work

Team:Communication

Colony-PCR

PCR of [http://partsregistry.org/Part:BBa_I9030 BBa_I9030] and two control,[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)).

DNA Template	1
dNTP mix	5
Foward Primer	5
Reverse Primer	5
DNA polymerase	1
Thermopol Buffer	5
dH₂O	33
TOTAL	50μL

95°C,5min -> ( 95°C,1min -> 54°C,1min -> 72°C,1min )･･･25cycles -> 72°C,10min -> 6°C store

--->Gel Check

Sample DNA	1
Loading Dye	1
dH₂O	4
TOTAL	6μl

From left;

1 [http://partsregistry.org/Part:BBa_I9030 BBa_I9030] without LVA -> OK

2 [http://partsregistry.org/Part:BBa_I9030 BBa_I9030] Positive Control -> OK

3 [http://partsregistry.org/Part:BBa_F2620 BBa_F2620] Positive control -> OK

Gel Check Sample DNA : [http://partsregistry.org/Part:BBa_R0010 BBa_R0010]

Sample DNA	1
Loading Dye	1
dH₂O	4
TOTAL	6μl

Digestion Test

Sample DNA:

1:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010],2:[http://partsregistry.org/Part:BBa_S03154 BBa_S03154],3:[http://partsregistry.org/Part:BBa_J04500 BBa_J04500],4:[http://partsregistry.org/Part:BBa_C0161 BBa_C0161],5:[http://partsregistry.org/Part:BBa_C0261 BBa_C0261]

Sample No.	1	2	3	4	5
Sample DNA	1	1	1	1	1
PstI	0.5	0.5	0.5	0.5	0.5
SpeI	0.5	-	1	-	-
XbaI	-	0.5	-	0.5	0.5
Buffer 2	2	-	3	-	-
Buffer 3	-	6	-	6	6
(10×)BSA	2	0.6	3	-	-
(100×)BSA	-	-	-	0.6	0.6
dH₂O	3	6.4	7	1.4	1.4
TOTAL	20μl	60μl	30μl	60μl	60μl

Team:Output