Revision as of 23:52, 18 July 2008

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Things we did today

Margaret

Margaret

Grace

Gel OK this time (95V for 50 min; ladder resolved, loading dye didn't run funny)
Incorrect bands still for B0015, J04430, R0010; Krystle will redo the plasmid preps for these
Correct bands for GFP fusion and BB-pRL1383a. BB-pRL1383a band faint -- why?

name of person/people who performed the task

Summary of task and what was done. Link to experiment for detailed notes if necessary.
e.g. read through papers, worked on proposal, etc.

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 13: / Line 13: @@
 :*for details please look [[Team:Hawaii/Initial E. Coli Transformation|here]].
+=== Sequencing===
+:<strong>Grace</strong>
+:* Redid PCR reactions for GFP fusion, BB-pRL1383a, B0015, J04430, R0010
+::* Gel OK this time (95V for 50 min; ladder resolved, loading dye didn't run funny)
+::* Incorrect bands still for B0015, J04430, R0010; Krystle will redo the plasmid preps for these
+::* Correct bands for GFP fusion and BB-pRL1383a. BB-pRL1383a band faint -- why?
+:* Treated PCR products w/ [[Team:Hawaii/Protocols/ExoSAP|ExoSAP]]
+:* Determined DNA concentrations using nanodrop spectrometer
+:* Sent 11 samples to CORE Hawaii for sequencing
 == Drylab Work ==