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Team:Hawaii/Notebook/2008-07-18
From 2008.igem.org
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::* Correct bands for GFP fusion and BB-pRL1383a. BB-pRL1383a band faint -- why? | ::* Correct bands for GFP fusion and BB-pRL1383a. BB-pRL1383a band faint -- why? | ||
:* Treated PCR products w/ [[Team:Hawaii/Protocols/ExoSAP|ExoSAP]] | :* Treated PCR products w/ [[Team:Hawaii/Protocols/ExoSAP|ExoSAP]] | ||
| - | :* Determined DNA concentrations using nanodrop spectrometer | + | :* Determined DNA concentrations using nanodrop spectrometer (measured twice) |
| - | :* Sent | + | {| border="1" align="center" |
| + | |+''' DNA concentrations of PCR products''' | ||
| + | !PCR Sample | ||
| + | !Concentration (1st measurement) | ||
| + | !Concentration (2nd measurement) | ||
| + | |- | ||
| + | | align="center"|nir | ||
| + | | align="center"|1015 ng/μl || align="center"|1010 ng/μl | ||
| + | |- | ||
| + | | align="center"|slr2016-1 | ||
| + | |align="center"|1295 ng/μl | ||
| + | |align="center"|1107 ng/μl | ||
| + | |- | ||
| + | | align="center"|slr2016-2 | ||
| + | | align="center"|1518 ng/μl|| align="center"|1266 ng/μl | ||
| + | |- | ||
| + | | align="center"|pilA | ||
| + | | align="center"|1312 ng/μl|| align="center"|1330 ng/μl | ||
| + | |- | ||
| + | | align="center"| B0024 | ||
| + | | align="center"|1546 ng/μl|| align="center"|1474 ng/μl | ||
| + | |- | ||
| + | | align="center"|B0034 | ||
| + | | align="center"|1537 ng/μl|| align="center"|1386 ng/μl | ||
| + | |- | ||
| + | | align="center"|C0012 | ||
| + | | align="center"|2130 ng/μl|| align="center"|1530 ng/μl | ||
| + | |- | ||
| + | | align="center"|E0040 | ||
| + | | align="center"|1502 ng/μl|| align="center"|1236 ng/μl | ||
| + | |- | ||
| + | | align="center"|J33207 | ||
| + | | align="center"|1454 ng/μl|| align="center"|1223 ng/μl | ||
| + | |- | ||
| + | | align="center"|BB-pRL1383a | ||
| + | | align="center"|2279 ng/μl|| align="center"|2129 ng/μl | ||
| + | |- | ||
| + | | align="center"|GFP fusion | ||
| + | | align="center"|1660 ng/μl|| align="center"|1714 ng/μl | ||
| + | |} | ||
| + | :* Sent 22 samples to CORE Hawaii for sequencing | ||
== Drylab Work == | == Drylab Work == | ||
Revision as of 01:57, 19 July 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
PCR of pRL1383a Parts
- Margaret
- for details please look here.
Extraction of Biobricks and Transformation in DB3.1
- Margaret
- for details please look here.
Sequencing
- Grace
- Redid PCR reactions for GFP fusion, BB-pRL1383a, B0015, J04430, R0010
- Gel OK this time (95V for 50 min; ladder resolved, loading dye didn't run funny)
- Incorrect bands still for B0015, J04430, R0010; Krystle will redo the plasmid preps for these
- Correct bands for GFP fusion and BB-pRL1383a. BB-pRL1383a band faint -- why?
- Treated PCR products w/ ExoSAP
- Determined DNA concentrations using nanodrop spectrometer (measured twice)
| PCR Sample | Concentration (1st measurement) | Concentration (2nd measurement) |
|---|---|---|
| nir | 1015 ng/μl | 1010 ng/μl |
| slr2016-1 | 1295 ng/μl | 1107 ng/μl |
| slr2016-2 | 1518 ng/μl | 1266 ng/μl |
| pilA | 1312 ng/μl | 1330 ng/μl |
| B0024 | 1546 ng/μl | 1474 ng/μl |
| B0034 | 1537 ng/μl | 1386 ng/μl |
| C0012 | 2130 ng/μl | 1530 ng/μl |
| E0040 | 1502 ng/μl | 1236 ng/μl |
| J33207 | 1454 ng/μl | 1223 ng/μl |
| BB-pRL1383a | 2279 ng/μl | 2129 ng/μl |
| GFP fusion | 1660 ng/μl | 1714 ng/μl |
- Sent 22 samples to CORE Hawaii for sequencing
Drylab Work
Project Description (Abstract)
- Grace, Krystle, Margaret
- Wrote description of project (abstract)
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
