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Team:Hawaii/Experiment
From 2008.igem.org
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* [[Team:Hawaii/PCC6803 Growth Optimization | PCC6803 Growth Optimization]] | * [[Team:Hawaii/PCC6803 Growth Optimization | PCC6803 Growth Optimization]] | ||
* [[Team:Hawaii/PCR Amplification of pRL1383a| PCR Amplification of pRL1383a]] | * [[Team:Hawaii/PCR Amplification of pRL1383a| PCR Amplification of pRL1383a]] | ||
| - | * [[Team:Hawaii/ | + | * [[Team:Hawaii/Ligation of pRL1383a Parts| Ligation of pRL1383a Parts]] |
* [[Team:Hawaii/Biobrick_conversions|Conversion of GFP into a fusion brick and pRL1383a into a Biobrick vector]] | * [[Team:Hawaii/Biobrick_conversions|Conversion of GFP into a fusion brick and pRL1383a into a Biobrick vector]] | ||
* [[Team:Hawaii/Construction of BioBrick intermediates|Construction of BioBrick intermediates]] | * [[Team:Hawaii/Construction of BioBrick intermediates|Construction of BioBrick intermediates]] | ||
Revision as of 02:37, 6 August 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
In Progress
- PCC6803 Growth Optimization
- PCR Amplification of pRL1383a
- Ligation of pRL1383a Parts
- Conversion of GFP into a fusion brick and pRL1383a into a Biobrick vector
- Construction of BioBrick intermediates
- Construction of Broad-Host-Range Expression Vector
- Extraction of BioBrick Parts from Registry
Not Started
Completed
- PCC6803 cell density test
- PCC6803 Cryostock Thaw Test
- Effect of CO2 on Growth Experiment
- Effect of Water Motion on Clumping of Liquid Cultures
- Make Competent E. Coli
- Test Competent E. Coli
- Initial E. Coli Transformation
- Initial BioBrick Plasmid Extraction From Filter Paper
- Biobricks extraction/transformation competency test
- Initial PCC6803 Transformation
- Tri-parental conjugation between E. coli and PCC6803
- Mini Plasmid Prep
- Large-Scale Preparation of Plasmid
- Initial Synthetic Oligonucleotide Assembly
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
