Team:Paris/Notebook/Freezer

From 2008.igem.org

(Difference between revisions)

Revision as of 15:21, 15 August 2008

PCR products

PCR products
Number	Name	Oligo F	Oligo R	Template	Length	Comments	Product Left ?	[]µg/µL	Date
PCR124	pFlgA	O100	O101	-	261pb		yes	0.5	August 7
PCR125	pFlgB	O102	O103	-	261pb		yes	0.5	August 7
PCR126	pFlhB	O108	O109	-	260pb		yes	0.5	August 7
PCR127	flhDC	O131	O132	MG1655			no		August 8
PCR128	OmpR*	O138	O139	Strain OmpR*	700pb		yes	150	August 8
PCR129	EnvZ	O126	O127	Strain EnvZ*	1400pb		yes	100	August 8
PCR130	E0240 rbs+	O141	O140	MP143	900pb		no		August 11
PCR131	flhD rbs-	O132	O133	MG1655	350pb		no		August 11
PCR132	pFlhD rbs-	O130	O131	MG1655	600pb		no		August 11
PCR133	flhDC + prom	O110	O131	MG1655	1300pb		no		August 11
PCR134	flhDC + prom	O111	O131	MG1655			no		August 11
PCR135	pfliL	O124	O125	MG1655			yes		August 11
PCR136	pFlhDC	O110	O113	MG1655			yes		August 11
PCR137	pFlhDC	O111	O113	MG1655			yes		August 11

Plasmids: MiniPrep Products

Plasmids: MiniPrep Products 2008
Number	Name	Biobricks	Description	Products in use	Date
MP100	[http://partsregistry.org/Part:BBa_B0034 B0034 ] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		Strongest RBS (Efficiency = 1)		July 26
MP101	[http://partsregistry.org/Part:BBa_J23101 J23101] in [http://partsregistry.org/Part:BBa_J61002 pJ61002 ]		Strongest Constitutive promoter	July 26	July 22, July 23, July 26
MP102	[http://partsregistry.org/Part:BBa_J23109 J23109] in [http://partsregistry.org/Part:BBa_J61002 pJ61002 ]		Weak Constitutive promoter	July 26	July 22, July 26
MP103	[http://partsregistry.org/Part:BBa_R0079 R0079] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		promoter pLas (lasr & PAI regulated)	July 26	July 23, July 26
MP104	[http://partsregistry.org/Part:BBa_R0040 R0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		tetR repressible promoter	July 26	July 23, July 26
MP105	[http://partsregistry.org/Part:BBa_S03154 S03154] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		B0034(rbs) ->LasI		July 26
MP106	[http://partsregistry.org/Part:BBa_S03879 S03879] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		B0034(rbs) -> TetR		July 26
MP107	[http://partsregistry.org/Part:BBa_C0079 C0079] in [http://partsregistry.org/Part:pSB2K3 pSB2K3]		lasR activator with LVA Tag		July 23
MP108	[http://partsregistry.org/Part:BBa_C0179 C0179] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		lasR activator	July 26	July 22, July 23, July 26
MP109	[http://partsregistry.org/Part:BBa_E0030 E0030] in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]		YFP		July 26
MP110	[http://partsregistry.org/Part:BBa_E0040 E0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		GFP	July 26	July 23, July 26
MP111	[http://partsregistry.org/Part:BBa_E1010 E1010 ] in [http://partsregistry.org/Part:pSB2K3 pSB2K3]		mRFP	July 26	July 23, July 26
MP112	OmpR M571				July 23
MP113	EnvZ I2815				July 23
MP114	[http://partsregistry.org/Part:BBa_C0040 C0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		tetR repressor		July 23
MP115	[http://partsregistry.org/Part:pSB1A3 pSB1A3]				July 23
MP116	[http://partsregistry.org/Part:BBa_J23100 pJ23100] in [http://partsregistry.org/Part:BBa_J61002 J61002]		Strong constitutive promoter in J61002		July 26
MP117	[http://partsregistry.org/Part:BBa_J23107 pJ23107] in [http://partsregistry.org/Part:BBa_J61002 J61002]		Medium constitutive promoter in J61002	July 26	July 24, July 26
MP118	[http://partsregistry.org/Part:BBa_B0015 B0015] in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]		Double terminator	July 26	July 24, July 26
MP119	[http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:pSB2K3 pSB2K3]		AraC pBAD	July 26	July 24, July 26
MP120	[http://partsregistry.org/Part:BBa_B0030 B0030] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		Strong RBS (Efficiency = 0,6)		July 26
MP121	[http://partsregistry.org/Part:BBa_E0422 E0422] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		ECFP (RBS+LVA+Term)	July 26	July 24, July 26
MP122	[http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		gfp tri-part; strong rbs		July 26
MP123	[http://partsregistry.org/Part:J61002 J61002]				August 5
MP124	L102 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		Strong rbs - YFP D129 (BV) - D118 (BI)		August 1
MP125	L103 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		Strong rbs - mRFP D129 (BV) - D122 (BI)		August 1
MP126	L104 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		Strong rbs - lasR activator D129 (BV) - D114 (BI)		August 1
MP127	L105 in [http://partsregistry.org/Part:J61002 J61002]		Strong promoter - ECFP D123 (BV) - D130 (BI)		August 1
MP128	L106 in [http://partsregistry.org/Part:J61002 J61002]		Strong promoter - gfp Tripart D123 (BV) - D131 (BI)		August 1
MP129	L107 in [http://partsregistry.org/Part:J61002 J61002]		Strong promoter - ECFP D103 (BV) - D130 (BI)		August 1
MP130	L108 in [http://partsregistry.org/Part:J61002 J61002]		Strong promoter - gfp Tripart D103 (BV) - D131 (BI)		August 1
MP131	L110 in [http://partsregistry.org/Part:J61002 J61002]		Medium promoter - gfp Tripart D124 (BV) - D131 (BI)		August 1
MP132	L111 in [http://partsregistry.org/Part:J61002 J61002]		Weak promoter - ECFP D104 (BV) - D130 (BI)		August 1
MP133	L112 in [http://partsregistry.org/Part:J61002 J61002]		Weak promoter - GFP tripart D104 (BV) - D131 (BI)		August 1
MP134	L115 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		pLas - ECFP D105 (BV) - D130 (BI)		August 1
MP135	L116 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		pLas - gfp Tripart D105 (BV) - D131 (BI)		August 1
MP136	L117 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]		yfp - Double Terminator D125 (FV) - D117 (FI)		August 1
MP137	L118 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]		rfp - Double Terminator D125 (FV) - D121 (FI)		August 1
MP138	L119 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]		lasR activator + LVA - Double Terminator D125 (FV) - D113 (FI)		August 1
MP139	L121 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		pTet - gfp tripart D106 (BV) - D131 (BI)		August 1
MP140	L124 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		Strongest RBS - rfp D102 (BV) - D122 (BI)		August 1
MP141	L125 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		Strongest RBS - yfp D102 (BV) - D118 (BI)		August 1
MP142	[http://partsregistry.org/Part:pSB3K3 pSB3K3]				August 8
MP143	[http://partsregistry.org/Part:E0240 E0240] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		gfp generator		August 8
MP144	L128 in [http://partsregistry.org/Part:J61002 J61002]		pFlgA-RFP D132 (FI) - D136 (FV)	MP144.3 ; MP144.4	August 12
MP145	L129 in [http://partsregistry.org/Part:J61002 J61002]		pFlgB-RFP D133 (FI) - D136 (FV)	MP145.6 ; MP145.7	August 12
MP146	L130 in [http://partsregistry.org/Part:J61002 J61002]		pFlhB-RFP D134 (FI) - D136 (FV)	MP146.1 ; MP146.2	August 12
MP147	L100 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		rbs TetR - ECFP D110 (BV) - D130 (BI)		August 13
MP148	L101 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		rbs TetR - GFP tripart D110 (BV) - D131 (BI)	MP148.2 ; MP148.3	August 13
MP149	L114 in [http://partsregistry.org/Part:pSB2K3 pSB2K3]		AracpBAD - gfp tripart D126 (BV) - D131 (BI)		August 13
MP150	L120 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		tetR repressible promoter - ECFP D106 (BV) - D130 (BI)		August 13
MP151	L122 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		RBS-lasI - ECFP D107 (BV) - D130 (BI)		August 13
MP152	L123 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		RBS lasI - gfp tripart D107 (BV) - D131 (BI)		August 13
MP153	L126 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		Strongest RBS (1)- LasR activator (+LVA) D102 (BV) - D114 (BI)	MP153.1 ; MP153.3	August 13
MP154	L132 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		flhDC D142(FV) - D139(FI)		August 13
MP155	L133 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		OmpR* D142(FV) - D140(FI)		August 13
MP156	L134 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		EnvZ* D142(FV) - D141(FI)		August 13
MP157	L138 in [http://partsregistry.org/Part:pSB3K3 pSB3K3]		gfp generator (E0240) D137(FV) - D138(FI)		August 13
MP158	L139				August 15
MP159	L141				August 15
MP160	L142				August 15

Digestion Products

(Legend: Vector, Insert) Ø

Digestion Products
Number	Product Name	Matrix Name	Enzyme 1	Enzyme 2	Obs	Size Matrix	Size BB	Description	Product Left	[]ng/µL	Date
D100.1	[http://partsregistry.org/Part:BBa_B0034 B0034]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	34			10	July 26
D100.2	[http://partsregistry.org/Part:BBa_B0034 B0034]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	34			10	July 26
D101	[http://partsregistry.org/Part:BBa_B0034 B0034]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	XbaI	FV	2076	15			10	July 29
D102.1	[http://partsregistry.org/Part:BBa_B0034 B0034]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2077	14			10	July 26
D102.2	[http://partsregistry.org/Part:BBa_B0034 B0034]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2077	14			10	July 29
D103.1	[http://partsregistry.org/Part:BBa_J23101 J23101]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2100	883			10	July 26
D103.2	[http://partsregistry.org/Part:BBa_J23101 J23101]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2100	883			10	July 26
D104.1	[http://partsregistry.org/Part:BBa_J23109 J23109]	[http://partsregistry.org/Part:BBa_J61002 J61002]	SpeI	PstI	BV	2100	883			20	July 26
D104.2	[http://partsregistry.org/Part:BBa_J23109 J23109]	[http://partsregistry.org/Part:BBa_J61002 J61002]	SpeI	PstI	BV	2100	883			20	July 26
D105.1	[http://partsregistry.org/Part:BBa_R0079 R0079]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2222	14			5	July 26
D105.2	[http://partsregistry.org/Part:BBa_R0079 R0079]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2222	14			5	July 29
D106.1	[http://partsregistry.org/Part:BBa_R0040 R0040]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2119	14			8	July 26
D106.2	[http://partsregistry.org/Part:BBa_R0040 R0040]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2119	14			5	July 29
D107	[http://partsregistry.org/Part:BBa_S03154 S03154]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2750	14			5	July 26
D108.1	[http://partsregistry.org/Part:BBa_S03154 S03154]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	707			5	July 26
D108.2	[http://partsregistry.org/Part:BBa_S03154 S03154]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	707			5	July 29
D109	[http://partsregistry.org/Part:BBa_S03154 S03154]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	SpeI	FI	2056	708			5	July 26
D110	[http://partsregistry.org/Part:BBa_S03879 S03879]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2768	14			20	July 26
D111.1	[http://partsregistry.org/Part:BBa_S03879 S03879]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	725			20	July 26
D111.2	[http://partsregistry.org/Part:BBa_S03879 S03879]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	725			20	July 29
D112	[http://partsregistry.org/Part:BBa_S03879 S03879]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	SpeI	FI	2756	726			20	July 26
D113	[http://partsregistry.org/Part:BBa_C0079 C0079]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	EcoRI	SpeI	FI	4402	779			20	July 26
D114	[http://partsregistry.org/Part:BBa_C0079 C0079]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	XbaI	PstI	BI	4003	778			20	July 26
D115.1	[http://partsregistry.org/Part:BBa_C0179 C0179]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	SpeI	FI	4402	746			-	July 29
D115.2	[http://partsregistry.org/Part:BBa_C0179 C0179]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	SpeI	FI	4402	746			-	July 29
D116.1	[http://partsregistry.org/Part:BBa_C0179 C0179]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	4403	745			20	July 29
D116.1	[http://partsregistry.org/Part:BBa_C0179 C0179]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	4403	745			20	July 29
D117	[http://partsregistry.org/Part:BBa_E0030 E0030]	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]	EcoRI	SpeI	FI	3166	746			5	July 29
D118	[http://partsregistry.org/Part:BBa_E0030 E0030]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	XbaI	PstI	BI	3167	745			20	July 26
D119	[http://partsregistry.org/Part:BBa_E0040 E0040]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	SpeI	FI	2056	743			10	July 26
D120	[http://partsregistry.org/Part:BBa_E0040 E0040]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	742			10	July 26
D121	[http://partsregistry.org/Part:BBa_E1010 E1010]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	EcoRI	SpeI	FI	4402	704			10	July 26
D122	[http://partsregistry.org/Part:BBa_E1010 E1010]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	4403	703			10	July 26
D123.1	[http://partsregistry.org/Part:BBa_J23100 J23100]	[http://partsregistry.org/Part:J61002 J61002]	SpeI	PstI	BV	2100	883			10	July 26
D123.2	[http://partsregistry.org/Part:BBa_J23100 J23100]	[http://partsregistry.org/Part:J61002 J61002]	SpeI	PstI	BV	2100	883			10	July 26
D124.1	[http://partsregistry.org/Part:BBa_J23107 J23107]	[http://partsregistry.org/Part:J61002 J61002]	SpeI	PstI	BV	2100	883			15	July 26
D124.2	[http://partsregistry.org/Part:BBa_J23107 J23107]	[http://partsregistry.org/Part:J61002 J61002]	SpeI	PstI	BV	2100	883			15	July 26
D125.1	[http://partsregistry.org/Part:BBa_B0015 B0015]	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]	EcoRI	XbaI	FV	3303	15			15	July 26
D125.2	[http://partsregistry.org/Part:BBa_B0015 B0015]	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]	EcoRI	XbaI	FV	3303	15			15	July 26
D126.1	[http://partsregistry.org/Part:BBa_I0500 I0500]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	SpeI	PstI	BV	5621	14			10	July 26
D126.2	[http://partsregistry.org/Part:BBa_I0500 I0500]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	SpeI	PstI	BV	5621	14			10	July 26
D127.1	[http://partsregistry.org/Part:BBa_B0030 B0030]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	37			10	July 26
D127.2	[http://partsregistry.org/Part:BBa_B0030 B0030]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	37			10	July 26
D128.1	[http://partsregistry.org/Part:BBa_B0030 B0030]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	XbaI	FV	2079	15			10	July 29
D128.1	[http://partsregistry.org/Part:BBa_B0030 B0030]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	XbaI	FV	2079	15			10	July 29
D129.1	[http://partsregistry.org/Part:BBa_B0030 B0030]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2080	14			10	July 29
D129.2	[http://partsregistry.org/Part:BBa_B0030 B0030]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2080	14			10	July 29
D130.1	[http://partsregistry.org/Part:BBa_E0422 E0422]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	939			10	July 26
D130.2	[http://partsregistry.org/Part:BBa_E0422 E0422]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	939			10	July 26
D130.3	[http://partsregistry.org/Part:BBa_E0422 E0422]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	939			10	July 26
D131.1	[http://partsregistry.org/Part:BBa_E0840 E0840]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	900			10	July 26
D131.2	[http://partsregistry.org/Part:BBa_E0840 E0840]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	900			10	July 26
D131.3	[http://partsregistry.org/Part:BBa_E0840 E0840]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	900			10	July 26
D132	pFlgA	-	EcoRI	SpeI	FI	-	250			5	August 6
D133	pFlgB	-	EcoRI	SpeI	FI	-	250			5	August 6
D134	pFlhB	-	EcoRI	SpeI	FI	-	249			5	August 6
D135	pFlhDC	-	EcoRI	SpeI	FI	-		problem			August 6
D136	-	[http://partsregistry.org/Part:BBa_J61002 J61002]	EcoRI	SpeI	FV	2925	-				August 6
D137	pSB3K3	[http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3]	EcoRI	SpeI	FV	2727	-				August 6
D138	E0240	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	SpeI	FI	899	-				August 6
D139	flhDC	-	EcoRI	PstI	FI	-	972				August 6
D140	E040 rbs+	-	PstI	PstI	I	-					August 12
D141	flhD RBS -	-	EcoRI	SpeI	FI	-					August 12
D142	flhC RBS -	-	EcoRI	SpeI	FI	-					August 12
D143	flhDC + promoter	-	EcoRI	SpeI	FI	-					August 12
D144	pSB3K3	[http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3]	EcoRI	SpeI	FI	-					August 12
D145	pSB1A2	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	SpeI	FI	-					August 12
D146	MP148	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	1609	rbs TetR - GFP tripart D110 (BV) - D131 (BI)			August 15
D147	MP153	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	SpeI	FI	2056	799	Strongest RBS (1)- LasR activator (+LVA) D102 (BV) - D114 (BI)			August 15
D149	pfliL	-	EcoRI	SpeI	FI	-					August 14
D150	pfliL	-	XbaI	PstI	BI	-					August 14
D152	pSB3K3	[http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3]	XbaI	PstI	BI	-					August 14

Strains (glycerol stock)

Strains glycerol stock
Number	Name	Biobricks	Description	Verified clone	Date
S103	[http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3]		AraC pBAD		July 23
S106	[http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2]		ECFP (RBS+LVA+Term		July 23
S107	[http://partsregistry.org/Part:BBa_B0034 B0034] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		Strongest RBS (Efficiency = 1)		July 23
S108	[http://partsregistry.org/Part:BBa_R0079 R0079] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		promoter pLas (lasr & PAI regulated)		July 23
S109	[http://partsregistry.org/Part:BBa_J23101 J23101] in [http://partsregistry.org/Part:BBa_J61002 pJ61002 ]		Strongest Constitutive promoter		July 23
S110	[http://partsregistry.org/Part:BBa_J23109 J23109] in [http://partsregistry.org/Part:BBa_J61002 pJ61002 ]		Weak Constitutive promoter		July 23
S111	[http://partsregistry.org/Part:BBa_R0040 R0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		tetR repressible promoter		July 23
S112	[http://partsregistry.org/Part:BBa_S03154 S03154] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		B0034(rbs) ->LasI		July 23
S113	[http://partsregistry.org/Part:BBa_S03879 S03879] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		B0034(rbs) -> TetR		July 23
S114	[http://partsregistry.org/Part:BBa_C0079 C0079] in [http://partsregistry.org/Part:pSB2K3 pSB2K3]		lasR activator with LVA Tag		July 23
S115	[http://partsregistry.org/Part:BBa_C0179 C0179] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		lasR activator		July 23
S116	[http://partsregistry.org/Part:BBa_E0030 E0030] in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]		YFP		July 23
S117	[http://partsregistry.org/Part:BBa_E0040 E0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		GFP		July 23
S118	[http://partsregistry.org/Part:BBa_E1010 E1010] in [http://partsregistry.org/Part:pSB2K3 pSB2K3]		mRFP		July 23
S119	OmpR M571				July 23
S120	EnvZ I2815				July 23
S121	[http://partsregistry.org/Part:BBa_C0040 C0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		tetR repressor		July 23
S122	[http://partsregistry.org/Part:pSB1A3 pSB1A3]				July 23
S123	L102 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		Strong rbs - YFP D129 (BV) - D118 (BI)		Aug 1
S124	L103 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		Strong rbs - mRFP D129 (BV) - D122 (BI)		Aug 1
S125	L104 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		Strong rbs - lasR activator D129 (BV) - D114 (BI)		Aug 1
S126	L105 in [http://partsregistry.org/Part:J61002 J61002]		Strong promoter - ECFP D123 (BV) - D130 (BI)		Aug 1
S127	L106 in [http://partsregistry.org/Part:J61002 J61002]		Strong promoter - gfp Tripart D123 (BV) - D131 (BI)		Aug 1
S128	L107 in [http://partsregistry.org/Part:J61002 J61002]		Strongest promoter - ECFP D103 (BV) - D130 (BI)		Aug 1
S129	L108 in [http://partsregistry.org/Part:J61002 J61002]		Strong promoter - GFP tripart D103 (BV) - D131 (BI)		Aug 1
S130	L110 in [http://partsregistry.org/Part:J61002 J61002]		Medium promoter - gfp tripart D124 (BV) - D131 (BI)		Aug 1
S131	L111 in [http://partsregistry.org/Part:J61002 J61002]		Weak promoter - ECFP D104 (BV) - D130 (BI)		Aug 1
S132	L112 in [http://partsregistry.org/Part:J61002 J61002]		Weak promoter - gfp Tripart D104 (BV) - D131 (BI)		Aug 1
S133	L115 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		pLas - ECFP D105 (BV) - D130 (BI)		Aug 1
S134	L116 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		pLas - gfp Tripart D105 (BV) - D131 (BI)		Aug 1
S135	L117 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]		yfp - Double Terminator D125 (FV) - D117 (FI)		Aug 1
S136	L118 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]		rfp - Double Terminator D125 (FV) - D121 (FI)		Aug 1
S137	L119 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]		LasR activator + LVA - Double terminator D125 (FV) - D113 (FI)		Aug 1
S138	L121 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		pTet - gfp Tripart D106 (BV) - D131 (BI)		Aug 1
S139	L124 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		Strongest rbs - mRFP D102 (BV) - D122 (BI)		Aug 1
S140	L125 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		Strongest rbs - yfp D102 (BV) - D118 (BI)		Aug 1
S141	MG1655				Aug 7
S142	[http://partsregistry.org/Part:J61002 J61002]				Aug 7
S143	L128 in [http://partsregistry.org/Part:J61002 J61002]		pFlgA-RFP D132 (FI) - D136 (FV)	MP144.3 ; MP144.4	August 12
S144	L129 in [http://partsregistry.org/Part:J61002 J61002]		pFlgB-RFP D133 (FI) - D136 (FV)	MP145.6 ; MP145.7	August 12
S145	L130 in [http://partsregistry.org/Part:J61002 J61002]		pFlhB-RFP D134 (FI) - D136 (FV)	MP146.1 ; MP146.2	August 12
S146	L100 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		rbs TetR - ECFP D110 (BV) - D130 (BI)		August 13
S147	L101 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		rbs TetR - GFP tripart D110 (BV) - D131 (BI)		August 13
S148	L114 in [http://partsregistry.org/Part:pSB2K3 pSB2K3]		AracpBAD - gfp tripart D126 (BV) - D131 (BI)		August 13
S149	L120 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		tetR repressible promoter - ECFP D106 (BV) - D130 (BI)		August 13
S150	L122 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		RBS-lasI - ECFP D107 (BV) - D130 (BI)		August 13
S151	L123 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		RBS lasI - gfp tripart D107 (BV) - D131 (BI)		August 13
S152	L126 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		Strongest RBS (1)- LasR activator (+LVA) D102 (BV) - D114 (BI)		August 13
S153	L132 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		flhDC D142(FV) - D139(FI)		August 13
S154	L133 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		OmpR* D142(FV) - D140(FI)		August 13
S155	L134 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		EnvZ* D142(FV) - D141(FI)		August 13
S156	L138 in [http://partsregistry.org/Part:pSB3K3 pSB3K3]		gfp generator (E0240) D137(FV) - D138(FI)		August 13
S157					August 15
S158					August 15
S159	L139				August 15
S160	L141				August 15
S161	L142				August 15

@@ Line 1,714: / Line 1,714: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S123
-|align="center"|L102
+|align="center"|L102 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
 |align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]
 |align="center"|Strong rbs - YFP<br>D129 (BV) -  D118 (BI)
@@ Line 1,721: / Line 1,721: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S124
-|align="center"|L103
+|align="center"|L103 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
 |align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]
 |align="center"|Strong rbs - mRFP<br>D129 (BV) - D122 (BI)
@@ Line 1,728: / Line 1,728: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S125
-|align="center"|L104
+|align="center"|L104 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
 |align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]
 |align="center"|Strong rbs - lasR activator<br>D129 (BV) - D114 (BI)
@@ Line 1,735: / Line 1,735: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S126
-|align="center"|L105
+|align="center"|L105 in [http://partsregistry.org/Part:J61002 J61002]
 |align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
 |align="center"|Strong promoter - ECFP<br>D123 (BV) - D130 (BI)
@@ Line 1,742: / Line 1,742: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S127
-|align="center"|L106
+|align="center"|L106 in [http://partsregistry.org/Part:J61002 J61002]
 |align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
 |align="center"|Strong promoter -  gfp Tripart<br>D123 (BV) - D131 (BI)
@@ Line 1,749: / Line 1,749: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S128
-|align="center"|L107
+|align="center"|L107 in [http://partsregistry.org/Part:J61002 J61002]
 |align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
 |align="center"|Strongest promoter - ECFP<br>D103 (BV) - D130 (BI)
@@ Line 1,756: / Line 1,756: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S129
-|align="center"|L108
+|align="center"|L108 in [http://partsregistry.org/Part:J61002 J61002]
 |align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
 |align="center"|Strong promoter - GFP tripart<br>D103 (BV) - D131 (BI)
@@ Line 1,763: / Line 1,763: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S130
-|align="center"|L110
+|align="center"|L110 in [http://partsregistry.org/Part:J61002 J61002]
 |align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
 |align="center"|Medium promoter - gfp tripart<br>D124 (BV) - D131 (BI)
@@ Line 1,770: / Line 1,770: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S131
-|align="center"|L111
+|align="center"|L111 in [http://partsregistry.org/Part:J61002 J61002]
 |align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
 |align="center"|Weak promoter - ECFP<br>D104 (BV) - D130 (BI)
@@ Line 1,777: / Line 1,777: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S132
-|align="center"|L112
+|align="center"|L112 in [http://partsregistry.org/Part:J61002 J61002]
 |align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
 |align="center"|Weak promoter - gfp Tripart<br>D104 (BV) - D131 (BI)
@@ Line 1,784: / Line 1,784: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S133
-|align="center"|L115
+|align="center"|L115 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
 |align="center"|[[Image:Part_icon_reporter.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
 |align="center"|pLas - ECFP <br>D105 (BV) - D130 (BI)
@@ Line 1,791: / Line 1,791: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S134
-|align="center"|L116
+|align="center"|L116 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
 |align="center"|[[Image:Part_icon_reporter.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
 |align="center"|pLas - gfp Tripart<br>D105 (BV) - D131 (BI)
@@ Line 1,798: / Line 1,798: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S135
-|align="center"|L117
+|align="center"|L117 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
 |align="center"|[[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
 |align="center"|yfp - Double Terminator<br>D125 (FV) - D117 (FI)
@@ Line 1,805: / Line 1,805: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S136
-|align="center"|L118
+|align="center"|L118 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
 |align="center"|[[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
 |align="center"|rfp - Double Terminator<br>D125 (FV) - D121 (FI)
@@ Line 1,812: / Line 1,812: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S137
-|align="center"|L119
+|align="center"|L119 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
 |align="center"|[[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
 |align="center"|LasR activator + LVA - Double terminator<br>D125 (FV) - D113 (FI)
@@ Line 1,819: / Line 1,819: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S138
-|align="center"|L121
+|align="center"|L121 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
 |align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
 |align="center"|pTet - gfp Tripart<br>D106 (BV) - D131 (BI)
@@ Line 1,826: / Line 1,826: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S139
-|align="center"|L124
+|align="center"|L124 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
 |align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]
 |align="center"|Strongest rbs - mRFP<br>D102 (BV) - D122 (BI)
@@ Line 1,833: / Line 1,833: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S140
-|align="center"|L125
+|align="center"|L125 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
 |align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]
 |align="center"|Strongest rbs - yfp<br>D102 (BV) - D118 (BI)
@@ Line 1,854: / Line 1,854: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S143
-|align="center"|L128
+|align="center"|L128 in [http://partsregistry.org/Part:J61002 J61002]
 |align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
 |align="center"|pFlgA-RFP<br>D132 (FI) -  D136 (FV)
@@ Line 1,861: / Line 1,861: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S144
-|align="center"|L129
+|align="center"|L129 in [http://partsregistry.org/Part:J61002 J61002]
 |align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
 |align="center"|pFlgB-RFP<br>D133 (FI) -  D136 (FV)
@@ Line 1,868: / Line 1,868: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S145
-|align="center"|L130
+|align="center"|L130 in [http://partsregistry.org/Part:J61002 J61002]
 |align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
 |align="center"|pFlhB-RFP<br>D134 (FI) -  D136 (FV)
@@ Line 1,875: / Line 1,875: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S146
-|align="center"|L100
+|align="center"|L100 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
 |align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
 |align="center"|rbs TetR - ECFP<br>D110 (BV) -  D130 (BI)
@@ Line 1,882: / Line 1,882: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S147
-|align="center"|L101
+|align="center"|L101 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
 |align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
 |align="center"|rbs TetR - GFP tripart<br>D110 (BV) -  D131 (BI)
@@ Line 1,889: / Line 1,889: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S148
-|align="center"|L114
+|align="center"|L114 in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
 |align="center"| [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
 |align="center"|AracpBAD - gfp tripart<br>D126 (BV) - D131 (BI)
@@ Line 1,896: / Line 1,896: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S149
-|align="center"|L120
+|align="center"|L120 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
 |align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
 |align="center"|tetR repressible promoter - ECFP<br>D106 (BV) - D130 (BI)
@@ Line 1,903: / Line 1,903: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S150
-|align="center"|L122
+|align="center"|L122 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
 |align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
 |align="center"|RBS-lasI - ECFP <br>D107 (BV) - D130 (BI)
@@ Line 1,910: / Line 1,910: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S151
-|align="center"|L123
+|align="center"|L123 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
 |align="center"| [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
 |align="center"|RBS lasI - gfp tripart<br>D107 (BV) - D131 (BI)
@@ Line 1,917: / Line 1,917: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S152
-|align="center"|L126
+|align="center"|L126 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
 |align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]
 |align="center"|Strongest RBS (1)- LasR activator (+LVA)<br>D102 (BV) - D114 (BI)
@@ Line 1,924: / Line 1,924: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S153
-|align="center"|L132
+|align="center"|L132 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
 |align="center"|[[Image:Icon_coding.png]]
 |align="center"|flhDC <br> D142(FV) - D139(FI)
@@ Line 1,931: / Line 1,931: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S154
-|align="center"|L133
+|align="center"|L133 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
 |align="center"|[[Image:Icon_coding.png]]
 |align="center"|OmpR*<br> D142(FV) - D140(FI)
@@ Line 1,938: / Line 1,938: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S155
-|align="center"|L134
+|align="center"|L134 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
 |align="center"|[[Image:Icon_coding.png]]
 |align="center"| EnvZ*<br> D142(FV) - D141(FI)
@@ Line 1,945: / Line 1,945: @@
 |- style="background: #dddddd;"
 | style="background: #D4E2EF;" |S156
-|align="center"|L138
+|align="center"|L138 in [http://partsregistry.org/Part:pSB3K3 pSB3K3]
 |align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]]
 |align="center"|gfp generator (E0240)<br> D137(FV) - D138(FI)

Team:Paris/Notebook/Freezer

From 2008.igem.org

Revision as of 15:21, 15 August 2008

Contents

PCR products

Plasmids: MiniPrep Products

Digestion Products

Strains (glycerol stock)