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Team:KULeuven/22 August 2008
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=== Wet Lab === | === Wet Lab === | ||
* We made digests (with ''Eco''RI and ''Spe''I) of the following ligations: R0084+J23022+J23109+J23032, R0084+B0032, R0040+J23022 and K145001. After that, these digests were put on gel. They seemed to have been cut properly, but when we purified these parts from the gel the concentrations were very low. | * We made digests (with ''Eco''RI and ''Spe''I) of the following ligations: R0084+J23022+J23109+J23032, R0084+B0032, R0040+J23022 and K145001. After that, these digests were put on gel. They seemed to have been cut properly, but when we purified these parts from the gel the concentrations were very low. | ||
| - | * We also did some PCRs to construct BioBrick K145014 (T7 polymerase with UmuD tag) and to test the transduction. | + | * We also did some PCRs to construct BioBrick K145014 (T7 polymerase with UmuD tag) and to test the transduction, both PCRs failed. The PCR to test the transduction was set up again, now with less template. |
* We started a few ligations (the ones that failed before): C0040+B0015, K145015+B0015, C0012+B0015, C0060+B0015, K145001+B0015 and R0040+J23022. | * We started a few ligations (the ones that failed before): C0040+B0015, K145015+B0015, C0012+B0015, C0060+B0015, K145001+B0015 and R0040+J23022. | ||
* We MiniPrepped K145151 + B0015. | * We MiniPrepped K145151 + B0015. | ||
Revision as of 17:24, 24 August 2008
dock/undock dropdown
Contents |
Lab Work
Wet Lab
- We made digests (with EcoRI and SpeI) of the following ligations: R0084+J23022+J23109+J23032, R0084+B0032, R0040+J23022 and K145001. After that, these digests were put on gel. They seemed to have been cut properly, but when we purified these parts from the gel the concentrations were very low.
- We also did some PCRs to construct BioBrick K145014 (T7 polymerase with UmuD tag) and to test the transduction, both PCRs failed. The PCR to test the transduction was set up again, now with less template.
- We started a few ligations (the ones that failed before): C0040+B0015, K145015+B0015, C0012+B0015, C0060+B0015, K145001+B0015 and R0040+J23022.
- We MiniPrepped K145151 + B0015.
Dry Lab
Modeling
Wiki
Official super duper release of wiki homepage and global style, but needs fixes to match with annoying IE browser. Picture gallery fixes will have to wait.
Remarks
Tippenvulwedstrijd: Hanne: 1 min 29! Getting better!
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