"
Edinburgh/10 August 2008
From 2008.igem.org
(Difference between revisions)
(New page: <html> <head> <title>Edinburgh iGEM 2008</title> <script type="text/javascript"> //Drop Down Tabs Menu- Author: Dynamic Drive (http://www.dynamicdrive.com) //Created: May 16th, 07' var ta...) |
|||
| Line 271: | Line 271: | ||
<li><a href="https://2008.igem.org/Team:Edinburgh" title="Home"><span>Home</span></a></li> | <li><a href="https://2008.igem.org/Team:Edinburgh" title="Home"><span>Home</span></a></li> | ||
<li><a href="https://2008.igem.org/Team:Edinburgh/Project" title="Project" rel="dropmenu1_a"><span>The Project</span></a></li> | <li><a href="https://2008.igem.org/Team:Edinburgh/Project" title="Project" rel="dropmenu1_a"><span>The Project</span></a></li> | ||
| - | |||
| - | |||
<li><a href="https://2008.igem.org/Team:Edinburgh/Modeling" title="Modelling" rel="dropmenu2_a"><span>Modelling</span></a></li> | <li><a href="https://2008.igem.org/Team:Edinburgh/Modeling" title="Modelling" rel="dropmenu2_a"><span>Modelling</span></a></li> | ||
| - | <li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li> | + | <li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li> |
| + | <li><a href="https://2008.igem.org/Team:Edinburgh/Results" title="Results" rel="dropmenu1_a"><span>Results</span></a></li> | ||
| + | <li><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Edinburgh" title="Parts" rel="dropmenu1_a"><span>BioBrick Parts</span></a></li> | ||
| + | <li><a href="https://2008.igem.org/Team:Edinburgh/Team" title="Team" ><span>The Team</span></a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
| Line 289: | Line 290: | ||
</html> | </html> | ||
| - | === | + | <table> |
| - | ==== Sunday 10 August 08 | + | <html> |
| + | <style type="text/css"> | ||
| + | table.calendar { margin: 0; padding: 2px; } | ||
| + | table.calendar td { margin: 0; padding: 1px; vertical-align: top; } | ||
| + | table.month .heading td { padding:1px; background-color: black; color: white; text-align:center; font-size:140%; font-weight:bold; } | ||
| + | table.month .dow td { color:#000000; text-align:center; font-size:100%; } | ||
| + | table.month td.today { background-color:#cd0000; } | ||
| + | table.month td { | ||
| + | border: none; | ||
| + | margin: 0; | ||
| + | padding: 0pt 0.5pt; | ||
| + | font-weight: bold; | ||
| + | font-size: 9pt; | ||
| + | text-align: right; | ||
| + | background-color: white; | ||
| + | } | ||
| + | #bodyContent table.month a { background:none; padding:0 } | ||
| + | .day-active { color:#cd0000 } | ||
| + | .day-empty { color:#000000 } | ||
| + | |||
| + | </style> | ||
| + | </html> | ||
| + | |||
| + | |||
| + | {|style="font color="#ffffff"; "background-color:"#cd0000"; cellpadding="0" cellspacing="4" border="4" bordercolor="#000"; border-spacing:0px; text-align:"center" width="250px" | ||
| + | </table> | ||
| + | |||
| + | {| align="left" | ||
| + | |- | ||
| + | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=06}} | ||
| + | |- | ||
| + | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=07}} | ||
| + | |- | ||
| + | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=08}} | ||
| + | |} | ||
| + | |||
| + | :::: '''[[Edinburgh/8_August_2008|< Previous Entry]]''' | ||
| + | |||
| + | == Week 8 == | ||
| + | === Sunday 10 August 08 === | ||
* Extracted ''C. fimi'' genomic DNA, followed by PCR of ''rrnB'' ('''P61'''), ''cenA'' ('''P62''') and ''cex'' ('''P63''') from genomic DNA. (CF) | * Extracted ''C. fimi'' genomic DNA, followed by PCR of ''rrnB'' ('''P61'''), ''cenA'' ('''P62''') and ''cex'' ('''P63''') from genomic DNA. (CF) | ||
| Line 297: | Line 337: | ||
** Patches from Plate 101 (L31, glgC double mutant+pSB1A2) to '''Plate 103'''. | ** Patches from Plate 101 (L31, glgC double mutant+pSB1A2) to '''Plate 103'''. | ||
** Patches from Plates 105 (pSB1A2+''crtY'') and 106 (pSB1A2+rbs+''crtY'') to '''Plate 108'''. (CF) | ** Patches from Plates 105 (pSB1A2+''crtY'') and 106 (pSB1A2+rbs+''crtY'') to '''Plate 108'''. (CF) | ||
| - | * Transformed competent cells with '''L36''' pSB1A2+''crtY'' ('''Plates 104/105''') and '''L37''' pSB1A2+rbs+''crtY'' ('''Plates 106/107'''). (CF) | + | * Transformed competent cells with '''L36''' pSB1A2+''crtY'' ('''Plates 104/105''') and '''L37''' pSB1A2+rbs+''crtY'' ('''Plates 106/107'''). (CF)<br /> |
| + | <br /> | ||
| + | |||
| + | :::: '''[[Edinburgh/11_August_2008|Next Entry >]]''' | ||
Revision as of 10:20, 28 August 2008
| ||||||||||||||||||||||||||||||||||||||||||||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||
Week 8
Sunday 10 August 08
- Extracted C. fimi genomic DNA, followed by PCR of rrnB (P61), cenA (P62) and cex (P63) from genomic DNA. (CF)
- Made subcultures...
- Patches from Plate 99 (L32, crtB+crtI) to Plate 102.
- Patches from Plate 101 (L31, glgC double mutant+pSB1A2) to Plate 103.
- Patches from Plates 105 (pSB1A2+crtY) and 106 (pSB1A2+rbs+crtY) to Plate 108. (CF)
- Transformed competent cells with L36 pSB1A2+crtY (Plates 104/105) and L37 pSB1A2+rbs+crtY (Plates 106/107). (CF)
