Team:UNIPV-Pavia/Notebook/Week15

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'''08/26/08'''
'''08/26/08'''
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*We transformed/plated ligations.
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'''08/27/08'''
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*Plates grew correctly. We checked colony fluorescence under UV rays:
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**BBa_R0051-BBa_E0240 glowed
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**BBa_R0040-BBa_E0240 glowed
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**12-b (BBa_R0051-BBa_B0030-BBa_C0062-BBa_B0030-BBa_C0061-BBa_B1006-BBa_R0062-BBa_E0240) glowed

Revision as of 20:36, 22 September 2008


Home.jpg Home Unipv logo.jpg The Team And.jpg The Project Safety.jpg Biological Safety Dna.png Parts Submitted to the Registry
Laptop.jpg Dry Lab Pipette.jpg Wet Lab Math.gif Modeling Note.jpg Protocols Notebook.gif Activity Notebook



Notebook



Week 1 Week 2 Week 3 Week 4 Week 5 Week 6 Week 7
Week 8 Week 9 Week 10 Week 11 Week 12 Week 13 Week 14
Week 15 Week 16 Week 17 Week 18 Week 19 Week 20 Week 21



Week 15: 08/25/08 - 08/29/08

08/25/08

  • Pladmid digestion for:
    • BBa_R0051 (S-P)
    • BBa_R0040 (S-P)
    • b (E-X)
    • BBa_B1006 (E-X)
    • 12 (E-S)
  • Run/gel extraction.
  • Ligations:
    • BBa_R0051 (S-P) - BBa_E0240 (X-P) (for promoter test)
    • BBa_R0040 (S-P) - BBa_E0240 (X-P) (for promoter test)
    • 12 (E-S) - b (E-X) (for AND logic gate test)
    • 12 (E-S) - BBa_B1006 (E-X) (to re-perform mutated assemblies)
  • We incubated ligations at 16°C overnight.

08/26/08

  • We transformed/plated ligations.

08/27/08

  • Plates grew correctly. We checked colony fluorescence under UV rays:
    • BBa_R0051-BBa_E0240 glowed
    • BBa_R0040-BBa_E0240 glowed
    • 12-b (BBa_R0051-BBa_B0030-BBa_C0062-BBa_B0030-BBa_C0061-BBa_B1006-BBa_R0062-BBa_E0240) glowed
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