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Team:Chiba/jk/β/week 4
From 2008.igem.org
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===7 September, 2008=== | ===7 September, 2008=== | ||
-->(6/9) | -->(6/9) | ||
| + | [[Team:Chiba/protocol/digestion|Digestion Test]] | ||
| + | ::Sample DNA 1 : BBa_R0010(濃度未知)[http://partsregistry.org/Part:BBa_R0010] | ||
| + | ::Sample DNA 2 : BBa_R0010(100ng/μL)[http://partsregistry.org/Part:BBa_R0010] | ||
| + | |||
| + | <table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
| + | <tr> | ||
| + | <td width="257">Sample No</td> | ||
| + | <td>1,2</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Sample DNA</td> | ||
| + | <td>12</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>PstⅠ</td> | ||
| + | <td>0.5</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>SpeⅠ</td> | ||
| + | <td>0.5</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Buffer 2</td> | ||
| + | <td>2</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>(×10)BSA</td> | ||
| + | <td>2</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>dH<sub>2</sub>O</td> | ||
| + | <td>3</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>TOTAL</td> | ||
| + | <td>20</td> | ||
| + | </tr> | ||
| + | </table> | ||
===8 September, 2008=== | ===8 September, 2008=== | ||
Revision as of 08:32, 30 September 2008
>送受信班
Contents |
Week 4
>last week
7 September, 2008
-->(6/9) Digestion Test
- Sample DNA 1 : BBa_R0010(濃度未知)[http://partsregistry.org/Part:BBa_R0010]
- Sample DNA 2 : BBa_R0010(100ng/μL)[http://partsregistry.org/Part:BBa_R0010]
| Sample No | 1,2 |
| Sample DNA | 12 |
| PstⅠ | 0.5 |
| SpeⅠ | 0.5 |
| Buffer 2 | 2 |
| (×10)BSA | 2 |
| dH2O | 3 |
| TOTAL | 20 |
8 September, 2008
9 September, 2008
10 September, 2008
11 September, 2008
12 September, 2008
13 September, 2008
- Gel Check
- BBa_C0161[http://partsregistry.org/Part:BBa_Co161](2007)
- BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)
- BBa_R0010[http://partsregistry.org/Part:BBa_R0010](2007)
|
|
-->Digestion
- BBa_C0161[http://partsregistry.org/Part:BBa_Co161](2007)
- BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)
- BBa_R0010[http://partsregistry.org/Part:BBa_R0010](2007)
| Sample No | ① | ② | ③ | ④ |
| Sample DNA | 5 | 5 | 1 | 1 |
| EcoRⅠ | 0.5 | o.5 | - | - |
| SpeⅠ | 0.5 | 0.5 | - | - |
| Buffer 1 | 1 | 1 | - | - |
| Buffer 3 | - | - | 1 | - |
| BSA | 1 | 1 | - | - |
| dH2O | 2 | 2 | 7.5 | - |
| TOTAL | 10 | 10 | 10 | 1 |
>next week
>next week
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