"
Team:Chiba/Calendar-Home/7 September 2008
From 2008.igem.org
(Difference between revisions)
(→Team:Output) |
|||
| Line 76: | Line 76: | ||
===Team:Output=== | ===Team:Output=== | ||
| - | + | <big>Cloning<big> | |
[[Team:Chiba/protocol/digestion|Digestion]] | [[Team:Chiba/protocol/digestion|Digestion]] | ||
| - | *vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040] | + | *vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]① 75ng/μl |
| - | *insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158] | + | *insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158]② 33.6ng/μl |
| - | *insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156] | + | *insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156]③ 33.6ng/μl |
| - | *insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160] | + | *insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160]④ 38.4ng/μl |
| - | *vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] | + | *vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]⑤ 100ng/μl |
*insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]⑥ 33.6ng/μl | *insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]⑥ 33.6ng/μl | ||
| Line 124: | Line 124: | ||
<tr> | <tr> | ||
<td>TOTAL</td> | <td>TOTAL</td> | ||
| - | <td> | + | <td>60μl</td><td>70μl</td><td>70μl</td><td>60μl</td><td>20μl</td><td>70μl</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| Line 151: | Line 151: | ||
<tr> | <tr> | ||
<td>TOTAL</td> | <td>TOTAL</td> | ||
| - | <td> | + | <td>12μl</td><td>12μl</td><td>12μl</td><td>12μl</td><td>6μl</td><td>12μl</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
Revision as of 15:45, 24 October 2008
6 September 2008 <|> 8 September 2008
Contents |
Laboratory work
Team:Input
no work
Team:Communication
-->(6/9) Digestion Test
- Sample DNA 1 : BBa_R0010(unknown)[http://partsregistry.org/Part:BBa_R0010]
- Sample DNA 2 : BBa_R0010(100ng/μL)[http://partsregistry.org/Part:BBa_R0010]
| Sample No | 1,2 |
| Sample DNA | 12 |
| PstⅠ | 0.5 |
| SpeⅠ | 0.5 |
| Buffer 2 | 2 |
| (×10)BSA | 2 |
| dH2O | 3 |
| TOTAL | 20 |
- --->Gel Check
Sample left right Sample DNA 5 5 Loading Dye 1 1 TOTAL 6 6
- Left side:BBa_R0010 Sample No.1
- Right side:BBa_R0010 Sample No.2
-->(8/9)
Team:Output
Cloning Digestion
- vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]① 75ng/μl
- insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158]② 33.6ng/μl
- insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156]③ 33.6ng/μl
- insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160]④ 38.4ng/μl
- vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]⑤ 100ng/μl
- insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]⑥ 33.6ng/μl
Sample No. ① ② ③ ④ ⑤ ⑥ DNA(digested sample) 50 54 54 45 14 54 SpeⅠ 1 0 0 0 1 0 PstⅠ 0.5 0.5 0.5 0.5 0.5 0.5 XbaⅠ 0 0.5 0.5 0.5 0 0.5 BSA(x10) 0.6 7 7 6 2 7 Buffer2 6 - - - 2 - Buffer3 - 7 7 6 - 7 dH2O 1.9 1 1 2 0.5 1 TOTAL 60μl 70μl 70μl 60μl 20μl 70μl -->37℃ 3hour
Sample No. ① ② ③ ④ ⑤ ⑥ DNA(digested sample) 6 10 10 10 4 10 Loading Dye 2 2 2 2 1 2 dH2O 4 - - - 1 2 TOTAL 12μl 12μl 12μl 12μl 6μl 12μl well 10 7 7 6 5 7 -->DNA purification (①-④,⑥:7μl ⑤:6μl)
