Team:Chiba/Calendar-Home/7 September 2008

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(Difference between revisions)

Revision as of 02:04, 25 October 2008

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6 September 2008 <|> 8 September 2008

Laboratory work

Team:Input

no work

Team:Communication

-->(6/9) Digestion Test

Sample DNA 1 : [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](unknown)

Sample DNA 2 : [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](100ng/μL)

Sample No	1,2
Sample DNA(μL)	12
PstⅠ(μL)	0.5
SpeⅠ(μL)	0.5
Buffer 2(μL)	2
(×10)BSA(μL)	2
dH₂O(μL)	3
TOTAL(μL)	20

--->Gel Check

Sample	left	right
Sample DNA(μL)	5	5
Loading Dye(μL)	1	1
TOTAL(μL)	6	6

Left side:BBa_R0010 Sample No.1

Right side:BBa_R0010 Sample No.2

-->(8/9)

Team:Output

Cloning Digestion

vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]① 75ng/μl
insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158]② 33.6ng/μl
insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156]③ 33.6ng/μl
insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160]④ 38.4ng/μl
vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]⑤ 100ng/μl
insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]⑥ 33.6ng/μl

Sample No.	①	②	③	④	⑤	⑥
DNA(digested sample)(μL)	50	54	54	45	14	54
SpeⅠ(μL)	1	0	0	0	1	0
PstⅠ(μL)	0.5	0.5	0.5	0.5	0.5	0.5
XbaⅠ(μL)	0	0.5	0.5	0.5	0	0.5
BSA(x10)(μL)	0.6	7	7	6	2	7
Buffer2(μL)	6	-	-	-	2	-
Buffer3(μL)	-	7	7	6	-	7
dH₂O(μL)	1.9	1	1	2	0.5	1
TOTAL(μL)	60μl	70μl	70μl	60μl	20μl	70μl

-->37℃ 3hour

-->Gel Extraction

Sample No.	①	②	③	④	⑤	⑥
DNA(digested sample)(μL)	6	10	10	10	4	10
Loading Dye(μL)	2	2	2	2	1	2
dH₂O(μL)	4	-	-	-	1	2
TOTAL(μL)	12	12	12	12	6	12
well	10	7	7	6	5	7

-->DNA purification (①-④,⑥:7μl ⑤:6μl)

@@ Line 9: / Line 9: @@
 -->(6/9)
 [[Team:Chiba/protocol/digestion|Digestion Test]]
-::Sample DNA 1 : BBa_R0010(unknown)[http://partsregistry.org/Part:BBa_R0010]
+::Sample DNA 1 : [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](unknown)
-::Sample DNA 2 : BBa_R0010(100ng/μL)[http://partsregistry.org/Part:BBa_R0010]
+::Sample DNA 2 : [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](100ng/μL)
 <table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
@@ Line 18: / Line 18: @@
 </tr>
 <tr>
-<td>Sample DNA</td>
+<td>Sample DNA(μL)</td>
 <td>12</td>
 </tr>
 <tr>
-<td>PstⅠ</td>
+<td>PstⅠ(μL)</td>
 <td>0.5</td>
 </tr>
 <tr>
-<td>SpeⅠ</td>
+<td>SpeⅠ(μL)</td>
 <td>0.5</td>
 </tr>
 <tr>
-<td>Buffer 2</td>
+<td>Buffer 2(μL)</td>
 <td>2</td>
 </tr>
 <tr>
-<td>(×10)BSA</td>
+<td>(×10)BSA(μL)</td>
 <td>2</td>
 </tr>
 <tr>
-<td>dH<sub>2</sub>O</td>
+<td>dH<sub>2</sub>O(μL)</td>
 <td>3</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(μL)</td>
 <td>20</td>
 </tr>
@@ Line 56: / Line 56: @@
 </tr>
 <tr>
-<td>Sample DNA</td>
+<td>Sample DNA(μL)</td>
 <td>5</td><td>5</td>
 </tr>
 <tr>
-<td>Loading Dye</td>
+<td>Loading Dye(μL)</td>
 <td>1</td><td>1</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(μL)</td>
 <td>6</td><td>6</td>
 </tr>
@@ Line 91: / Line 91: @@
 </tr>
 <tr>
-<td>DNA(digested sample)</td>
+<td>DNA(digested sample)(μL)</td>
 <td>50</td><td>54</td><td>54</td><td>45</td><td>14</td><td>54</td>
 </tr>
 <tr>
-<td>SpeⅠ</td>
+<td>SpeⅠ(μL)</td>
 <td>1</td><td>0</td><td>0</td><td>0</td><td>1</td><td>0</td>
 </tr>
 <tr>
-<td>PstⅠ</td>
+<td>PstⅠ(μL)</td>
 <td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td>
 </tr>
 <tr>
-<td>XbaⅠ</td>
+<td>XbaⅠ(μL)</td>
 <td>0</td><td>0.5</td><td>0.5</td><td>0.5</td><td>0</td><td>0.5</td>
 </tr>
 <tr>
-<td>BSA(x10)</td>
+<td>BSA(x10)(μL)</td>
 <td>0.6</td><td>7</td><td>7</td><td>6</td><td>2</td><td>7</td>
 </tr>
 <tr>
-<td>Buffer2</td>
+<td>Buffer2(μL)</td>
 <td>6</td><td>-</td><td>-</td><td>-</td><td>2</td><td>-</td>
 </tr>
 <tr>
-<td>Buffer3</td>
+<td>Buffer3(μL)</td>
 <td>-</td><td>7</td><td>7</td><td>6</td><td>-</td><td>7<td>
 </tr>
 <tr>
-<td>dH<sub>2</sub>O</td>
+<td>dH<sub>2</sub>O(μL)</td>
 <td>1.9</td><td>1</td><td>1</td><td>2</td><td>0.5</td><td>1</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(μL)</td>
 <td>60μl</td><td>70μl</td><td>70μl</td><td>60μl</td><td>20μl</td><td>70μl</td>
 </tr>
@@ Line 138: / Line 138: @@
 </tr>
 <tr>
-<td>DNA(digested sample)</td>
+<td>DNA(digested sample)(μL)</td>
 <td>6</td><td>10</td><td>10</td><td>10</td><td>4</td><td>10</td>
 </tr>
 <tr>
-<td>Loading Dye</td>
+<td>Loading Dye(μL)</td>
 <td>2</td><td>2</td><td>2</td><td>2</td><td>1</td><td>2</td>
 </tr>
 <tr>
-<td>dH<sub>2</sub>O</td>
+<td>dH<sub>2</sub>O(μL)</td>
 <td>4</td><td>-</td><td>-</td><td>-</td><td>1</td><td>2</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(μL)</td>
-<td>12μl</td><td>12μl</td><td>12μl</td><td>12μl</td><td>6μl</td><td>12μl</td>
+<td>12</td><td>12</td><td>12</td><td>12</td><td>6</td><td>12</td>
 </tr>
 <tr>