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Brown: Team Resistance/26 October 2008
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| - | + | ==26 October 2008== | |
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| + | ====Biobricking work==== | ||
*Made new culture of the PVJ4 transformation so that a miniprep can be done. | *Made new culture of the PVJ4 transformation so that a miniprep can be done. | ||
*New culture of gene S105 part made. | *New culture of gene S105 part made. | ||
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| + | Began transforming promoters from the registry in order to ligate them to our cassette and create composite parts: | ||
| + | *pBAD prmoter | ||
| + | *IPTG inducible promoter | ||
| + | *Mercury inducible promoter | ||
| + | *Pu inducible promoter (xylene induced) | ||
| + | *T6 promoter, with activity modified by change in temperature | ||
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| + | Also re-transformed ligations of the pVJ4 cassette and the S105 cassette (both transformations unsuccessful in the past) | ||
*Will run a gel of the S105 gene restriction digest with EcoRI and SpeI for verification of part. The gel showed a faint band at the length we hoped for but there was no other band verifying the vector. We should have seen two bands but we only saw one. | *Will run a gel of the S105 gene restriction digest with EcoRI and SpeI for verification of part. The gel showed a faint band at the length we hoped for but there was no other band verifying the vector. We should have seen two bands but we only saw one. | ||
Revision as of 21:51, 27 October 2008
26 October 2008Biobricking work
Began transforming promoters from the registry in order to ligate them to our cassette and create composite parts:
Also re-transformed ligations of the pVJ4 cassette and the S105 cassette (both transformations unsuccessful in the past)
Conductivity Tests
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