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Team:Waterloo/Notebook/Protocols/Inoculation
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<big><b><u>Inoculation Protocol </u></b></big><br> | <big><b><u>Inoculation Protocol </u></b></big><br> | ||
Latest revision as of 22:54, 28 October 2008
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Inoculation Protocol
Materials
DNA Samples
Ampicillin (2µL/sample)
Kanamycin (2.5µL/sample)
Liquid LB Media (1 tube/sample)
Instructions
1. Label all tubes.
2. Do the following in the flow hood:
- Pipette the correct amount of antibiotic into the corresponding DNA tubes. 2 µL for ApR, 2.5 µL KmR.
- Dip the sterile stick into the DNA and into the corresponding media tube.
3. Incubate at 37 degrees celcius overnight on the agitator.
