Team:Paris/Modeling/f0

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(Difference between revisions)
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|GFP concentration at steady-state
|GFP concentration at steady-state
|nM
|nM
 +
|
|
|
|-
|-
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|value of the observed fluorescence
|value of the observed fluorescence
|au
|au
 +
|
|mean value of ~20 measures
|mean value of ~20 measures
|-
|-
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|nM.au<sup>-1</sup>
|nM.au<sup>-1</sup>
|(1/79.429)
|(1/79.429)
 +
|
|}
|}
</center>
</center>
That will directly give us &#131;0, which corresponds to &beta;<sub>72</sub> in the [[Team:Paris/Modeling/Synchronisation|equations]], if the ''pConstitutive'' is J23101.
That will directly give us &#131;0, which corresponds to &beta;<sub>72</sub> in the [[Team:Paris/Modeling/Synchronisation|equations]], if the ''pConstitutive'' is J23101.

Revision as of 08:39, 29 October 2008

Method & Algorithm : ƒ0


Specific Plasmid Characterisation for ƒ0
In the exponential phase of growth, at the steady state, we have

EqF0.jpg

param signification unit value comments
[exprJ23101] expression rate of
J23101 with RBS E0032 		nM.min-1
γGFP dilution-degradation rate
of GFP(mut3b) 		min-1
[GFP] GFP concentration at steady-state nM
(fluorescence) value of the observed fluorescence au mean value of ~20 measures
conversion conversion ratio between
fluorescence and concentration 		nM.au-1 (1/79.429)

That will directly give us ƒ0, which corresponds to β72 in the equations, if the pConstitutive is J23101.

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