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Team:Chiba/Calendar-Home/6 September 2008
From 2008.igem.org
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===Team:Communication=== | ===Team:Communication=== | ||
'''[[Team:Chiba/protocol/PCR|Colony-PCR]]''' | '''[[Team:Chiba/protocol/PCR|Colony-PCR]]''' | ||
| - | ::PCR of BBa_I9030 and two control, | + | ::PCR of [http://partsregistry.org/Part:BBa_I9030 BBa_I9030] and two control,[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)). |
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"> | :<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
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</table> | </table> | ||
:From left;<br> | :From left;<br> | ||
| - | :1 | + | :1 [http://partsregistry.org/Part:BBa_I9030 I9030] without LVA -> OK |
| - | :2 | + | :2 [http://partsregistry.org/Part:BBa_I9030 I9030] Positive Control -> OK |
| - | :3 | + | :3 [http://partsregistry.org/Part:BBa_F2620 F2620] Positive control -> OK |
'''[[Team:Chiba/protocol/gelcheck|Gel Check]]''' | '''[[Team:Chiba/protocol/gelcheck|Gel Check]]''' | ||
| - | Sample DNA : R0010 | + | Sample DNA : [http://partsregistry.org/Part:BBa_R0010 R0010] |
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"> | :<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
<tr> | <tr> | ||
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'''[[Team:Chiba/protocol/digestion|Digestion Test]]''' | '''[[Team:Chiba/protocol/digestion|Digestion Test]]''' | ||
:Sample DNA: | :Sample DNA: | ||
| - | 1:R0010,2:S03154,3:J04500,4:C0161,5:C0261 | + | 1:[http://partsregistry.org/Part:BBa_R0010 R0010],2:[http://partsregistry.org/Part:BBa_S03154 S03154],3:[http://partsregistry.org/Part:BBa_J04500 J04500],4:[http://partsregistry.org/Part:BBa_C0161 C0161],5:[http://partsregistry.org/Part:BBa_C0261 C0261] |
<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"> | <table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
Revision as of 19:19, 29 October 2008
5 September 2008 <|> 7 September 2008
Contents |
Meeting with UmeG
the ppt (in Japanese) for our weekly meeting
Laboratory work
Team:Input
no work
Team:Communication
- PCR of [http://partsregistry.org/Part:BBa_I9030 BBa_I9030] and two control,[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)).
DNA Template 1 dNTP mix 5 Foward Primer 5 Reverse Primer 5 DNA polymerase 1 Thermopol Buffer 5 dH2O 33 TOTAL 50μL
- 95°C,5min -> ( 95°C,1min -> 54°C,1min -> 72°C,1min )･･･25cycles -> 72°C,10min -> 6°C store
- --->Gel Check
Sample DNA 1 Loading Dye 1 dH2O 4 TOTAL 6 - From left;
- 1 [http://partsregistry.org/Part:BBa_I9030 I9030] without LVA -> OK
- 2 [http://partsregistry.org/Part:BBa_I9030 I9030] Positive Control -> OK
- 3 [http://partsregistry.org/Part:BBa_F2620 F2620] Positive control -> OK
Gel Check Sample DNA : [http://partsregistry.org/Part:BBa_R0010 R0010]
Sample DNA 1 Loading Dye 1 dH2O 4 TOTAL 6
- Sample DNA:
1:[http://partsregistry.org/Part:BBa_R0010 R0010],2:[http://partsregistry.org/Part:BBa_S03154 S03154],3:[http://partsregistry.org/Part:BBa_J04500 J04500],4:[http://partsregistry.org/Part:BBa_C0161 C0161],5:[http://partsregistry.org/Part:BBa_C0261 C0261]
Sample No. 1 2 3 4 5 Sample DNA 1 1 1 1 1 PstⅠ 0.5 0.5 0.5 0.5 0.5 SpeⅠ 0.5 - 1 - - XbaⅠ - 0.5 - 0.5 0.5 Buffer 2 2 - 3 - - Buffer 3 - 6 - 6 6 (10×)BSA 2 0.6 3 - - (100×)BSA - - - 0.6 0.6 dH2O 3 6.4 7 1.4 1.4 TOTAL 20 60 30 60 60 Team:Output
no work
- From left;
