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Team:Hawaii/Experiment
From 2008.igem.org
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* [[Team:Hawaii/Construction of Broad-Host-Range Expression Vector| Construction of Broad-Host-Range Expression Vector]] | * [[Team:Hawaii/Construction of Broad-Host-Range Expression Vector| Construction of Broad-Host-Range Expression Vector]] | ||
* [[Team:Hawaii/Construction of Omega Interposon BioBrick | Construction of Omega Interposon BioBrick]] | * [[Team:Hawaii/Construction of Omega Interposon BioBrick | Construction of Omega Interposon BioBrick]] | ||
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* [[Team:Hawaii/Antibiotic_test_for_aadA construct|Antibiotic test for ''aadA'' construct]] | * [[Team:Hawaii/Antibiotic_test_for_aadA construct|Antibiotic test for ''aadA'' construct]] | ||
* [[Team:Hawaii/Conjugation Test: Comparing oriT with oriTr |Conjugation Test: Comparing oriT with oriTr ]] | * [[Team:Hawaii/Conjugation Test: Comparing oriT with oriTr |Conjugation Test: Comparing oriT with oriTr ]] | ||
Revision as of 22:18, 29 October 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
In Progress
- Ligation of pRL1383a Parts
- Construction of BioBrick intermediates
- Construction of Broad-Host-Range Expression Vector
- Construction of Omega Interposon BioBrick
- Antibiotic test for aadA construct
- Conjugation Test: Comparing oriT with oriTr
Not Started
Completed
- PCR Amplification of pRL1383a
- PCC6803 cell density test
- PCC6803 Cryostock Thaw Test
- Effect of CO2 on Growth Experiment
- Effect of Water Motion on Clumping of Liquid Cultures
- Make Competent E. Coli
- Test Competent E. Coli
- Initial E. Coli Transformation
- Initial BioBrick Plasmid Extraction From Filter Paper
- Biobricks extraction/transformation competency test
- Initial PCC6803 Transformation
- Tri-parental conjugation between E. coli and PCC6803
- Mini Plasmid Prep
- Large-Scale Preparation of Plasmid
- Initial Synthetic Oligonucleotide Assembly
- Conversion of GFP into a fusion brick and pRL1383a into a Biobrick vector
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
