Team:KULeuven/4 August 2008

From 2008.igem.org

• Home
• Reports
• Calendar

• Project abstract
• Meet the team
• Leave a message
• Mail us

• The Team
  Research Labs and Groups Students Instructors Advisors Pictures
• The Project
  Summary Components

  Input Output Filter InverTimer Reset Cell Death Memory

  Safety End Evaluation Literature Brainstorm
• Ethics
  Introduction Three A's

  Defining the three A's Developments in modern physics Developments in biology

  Concerns & Issues

  Defining synthetic biology A framework Concerns Issues The need for answers

  Biological Robotics

  KULeuven iGEM 2008 project Biological robotics

  Afterword References
• Submitted Parts
  Sandbox
• Modeling
  Overview Kinetic Constants Components

  Output Filter InverTimer Reset Cell Death Memory

  Full Model Sensitivity Analysis Multi-cell Model Diffusion
• Data Analysis
  Overview New Parts

  GFP (LVA-tag) Other

  Components

  Input Output Other
• Software
  Multi-cell Toolbox Simbiology2LaTeX Toolbox
• Notebook
  Protocols Reports

  Daily Weekly

  Lab Data

  Freezer Primers Ligation

  Tools Press Acknowledgments

Lab Work

Wet Lab

• Competent cells were inoculated and prepared for electroporation with the folowing ligation products: C0040+B0015, J23109+J23032, I712074+J23032, E0022+B0015, P1010+B0015, C0060+B0015, C0062+B0015, R0084+B0032, J23100+B0032. The electroporation was performed and hopefully we will have transformed cells tomorrow.

• We continued to make the GFP-LVA Biobrick. The PCR product was put on gel and purified. It gave nice results,so the PCR worked well. Tomorrow we will cut it with EcoRI and SpeI.

• We made electrocompetent cells and tested them with pUC. Tomorrow we'll see if they're any good. A glycerolstock of the cells has been stored in the -80° freezer.

Dry Lab

Dries and Jonas started work on the Dr. Coli logo.

Modeling

We've made a phase plot of the memory to prove the existence of two distinct basins of attraction. Nick has looked into multi-cell interactions (multi-agent systems) a bit. Conclusion is that it might be a bit out of our scope.

Also decided to ignore cell division for now. The memory appears sufficiently stable to be passed on to the cells offspring. We suspect that the quantitites of our proteins and mRNA's should remain relatively stable.

Wiki

Remarks

• Recent changes
• What links here
• Related changes
• Special pages
• My preferences

• Printable version
• Permanent link
• Privacy policy
• Disclaimers