Team:KULeuven/3 September 2008

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Lab Work

Wet Lab

Once again, we tried to construct some of our new parts using PCR. It concerns the BioBricks K145014 (T7 polymerase with UmuD tag), K145150 (hybrid promoter) and K145013 (antisense LuxI).


We continued yesterday's digests. K145151+B0015, C0040+B0015, C0060+B0015, C0012+B0015 and K145015+B0015 were cut with EcoRI. They seemed alright.


We started a few ligations. This is step 2 of the ligation process, so they are a bit longer: (J23116+B0032)+(C0040+B0015), (R0040+B0032)+(K145015+B0015), (I712074+J23032)+(C0012+B0015), (I712074+J23032)+(C0060+B0015) and (R0040+J23022)+(J23109+J23032).


And finally, we also electroporated the ligations we made yesterday. Some of these ligations were electroporated into JM109 electrocompetent cells:

into TOP10 -> R0040+B0033, R0053+P0152, R0040+J23066, B0014+B0033, R0040+E0240, C0056+B0015, C0061+B0015 and
C0061+B0015 (PstI) - and of course pUC.
into JM109 -> C0056+B0015, C0061+B0015 and C0061+B0015 (PstI) - and pUC.

Dry Lab

Ethics... (re)-writing sections on the wiki... LaTeX output tool for MATLAB...

Modeling

Working out how we can make MATLAB remember diffusion of HSL in the medium during the simulation. Also investigating the scale at which this diffusion happens in the simulation

Wiki

Remarks

Jonathan came to visit us. He's very interested in the iGEM competition and it was his first visit to a team. We had a very interesting conversation and it was a really inspiring visit.

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