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From 2008.igem.org

Contents 1 Last ligation number 2 Last digestion number 3 PCR products 4 Plasmids: MiniPrep Products 5 Digestion Products 6 Strains (glycerol stock) Last ligation number Last Ligation number used 185 Last digestion number Last digestion number used PCR products PCR products Number Name Oligo F Oligo R Template Length Comments Product Left ? [DNA]µg/mL Date PCR124 pFlgA O100 O101 - 261pb yes 0.5 August 7 PCR125 pFlgB O102 O103 - 261pb yes 0.5 August 7 PCR126 pFlhB O108 O109 - 260pb yes 0.5 August 7 PCR127 flhDC O132 O131 MG1655 no August 8 PCR128 OmpR* O138 O139 Strain OmpR* 700pb yes 150 August 8 PCR129 EnvZ* O126 O127 Strain EnvZ* 1400pb yes 100 August 8 PCR130 E0240 rbs+ O141 O140 MP143 900pb no August 11 PCR131 flhD rbs- O132 O133 MG1655 350pb no August 11 PCR132 pFlhD rbs- O130 O131 MG1655 600pb no August 11 PCR133 flhDC + prom O110 O131 MG1655 1300pb no August 11 PCR134 flhDC + prom O111 O131 MG1655 no August 11 PCR135 pfliL O124 O125 MG1655 yes August 11 PCR136 pFlhDC O110 O113 MG1655 yes August 11 PCR137 pFlhDC O 111 O 113 MG1655 yes August 11 PCR138 FlhDC (RBS -) O 132 O 131 MG 1655 yes August 15 PCR139 pflhDC (URI) O 110 O 113 PCR 130 yes August 15 PCR140 flhDC (RBS -) O 132 O 131 PCR 130 yes August 15 PCR141 Gene FlhDC + rbs nat O 134 O 131 MG1655 1000pb yes August 26 PCR142 pFliA + rbs nat O 145 O 144 MG1655 325pb yes August 26 PCR143 pFliA sans rbs O 145 O 146 MG1655 310pb yes August 26 PCR144 pFliA + Gene FliA O 145 O 146 MG1655 1100pb yes August 26 PCR145 PCRFliA1 O 148 O 152 MG1655 150pb yes August 26 PCR146 PCRFliA2 O 153 O 150 MG1655 670pb yes August 26 PCR 147 EnvZ* O 126 O 127 S 120 1412 bp yes 150 August 26 PCR 148 OmpR* O 138 O 139 S 119 789 bp yes 101 August 26 PCR 151 pFLiA without nat. rbs O 145 O 146 MG1655 310 bp yes 34 August 26 PCR 152 pFliA rbs nat. O 145 O 144 MG1655 325 bp yes 19 August 26 PCR 153 pFliA+Gene FliA non-mutated O 145 O 151 MG1655 1100 bp yes 39 August 26 PCR 154 Gene FliA mutated (EcoRI/PstI) O 148 O 150 MG1655 800 bp 154.1, 154.2, 154.3 yes 50 August 26 Plasmids: MiniPrep Products Plasmids: MiniPrep Products 2008 Number Name Biobricks Description Products in use/comments Conc. ug/uL Date MP100 [http://partsregistry.org/Part:BBa_B0034 B0034 ] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strongest RBS (Efficiency = 1) July 26 MP101.1 [http://partsregistry.org/Part:BBa_J23101 J23101] in [http://partsregistry.org/Part:BBa_J61002 pJ61002 ] Strongest Constitutive promoter July 26 333 July 22, July 23, July 26 MP101.2 finished MP101.4 200 MP102 [http://partsregistry.org/Part:BBa_J23109 J23109] in [http://partsregistry.org/Part:BBa_J61002 pJ61002 ] Weak Constitutive promoter July 26 July 22, July 26 MP103 [http://partsregistry.org/Part:BBa_R0079 R0079] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] promoter pLas (lasr & PAI regulated) July 26 July 23, July 26 MP104.1 [http://partsregistry.org/Part:BBa_R0040 R0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] tetR repressible promoter July 26 145 July 23, July 26 MP104.3 147 MP104.4 51 MP105.1 [http://partsregistry.org/Part:BBa_S03154 S03154] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] B0034(rbs) ->LasI 34 July 26 MP105.2 31 MP106.1 [http://partsregistry.org/Part:BBa_S03879 S03879] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] B0034(rbs) -> TetR 80 August 24 MP106.3 40 MP107 [http://partsregistry.org/Part:BBa_C0079 C0079] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] lasR activator with LVA Tag July 23 MP108 [http://partsregistry.org/Part:BBa_C0179 C0179] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] lasR activator July 26 July 22, July 23, July 26 MP109 [http://partsregistry.org/Part:BBa_E0030 E0030] in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] YFP July 26 MP110 [http://partsregistry.org/Part:BBa_E0040 E0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] GFP July 26 July 23, July 26 MP111 [http://partsregistry.org/Part:BBa_E1010 E1010 ] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] mRFP July 26 July 23, July 26 MP112 OmpR M57I July 23 MP113 EnvZ I281S July 23 MP114.1 [http://partsregistry.org/Part:BBa_C0040 C0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] tetR repressor 173 July 23 MP114.2 263 MP115 [http://partsregistry.org/Part:pSB1A3 pSB1A3] July 23 MP116 [http://partsregistry.org/Part:BBa_J23100 pJ23100] in [http://partsregistry.org/Part:BBa_J61002 J61002] Strong constitutive promoter in J61002 July 26 MP117 [http://partsregistry.org/Part:BBa_J23107 pJ23107] in [http://partsregistry.org/Part:BBa_J61002 J61002] Medium constitutive promoter in J61002 July 26 July 24, July 26 MP118.1 [http://partsregistry.org/Part:BBa_B0015 B0015] in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Double terminator July 26 220 July 24, July 26 MP118.3 187 MP119.1 [http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] AraC pBAD July 26 42 July 24, July 26 MP119.2 26 MP119.3 39 MP120 [http://partsregistry.org/Part:BBa_B0030 B0030] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strong RBS (Efficiency = 0,6) July 26 MP121 [http://partsregistry.org/Part:BBa_E0422 E0422] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] ECFP (RBS+LVA+Double Term) July 26 July 24, July 26 MP122 [http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] gfp tri-part; strong rbs July 26 MP123 [http://partsregistry.org/Part:J61002 J61002] August 5 MP124 L102 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strong rbs - YFP D129 (BV) - D118 (BI) August 1 MP125 L103 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strong rbs - mRFP D129 (BV) - D122 (BI) August 1 MP126 L104 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strong rbs - lasR activator D129 (BV) - D114 (BI) August 1 MP127 L105 in [http://partsregistry.org/Part:J61002 J61002] Strong promoter - ECFP D123 (BV) - D130 (BI) August 1 MP128 L106 in [http://partsregistry.org/Part:J61002 J61002] Strong promoter - gfp Tripart D123 (BV) - D131 (BI) August 1 MP129 L107 in [http://partsregistry.org/Part:J61002 J61002] Strong promoter - ECFP D103 (BV) - D130 (BI) August 1 MP130 L108 in [http://partsregistry.org/Part:J61002 J61002] Strong promoter - gfp Tripart D103 (BV) - D131 (BI) August 1 MP131 L110 in [http://partsregistry.org/Part:J61002 J61002] Medium promoter - gfp Tripart D124 (BV) - D131 (BI) August 1 MP132 L111 in [http://partsregistry.org/Part:J61002 J61002] Weak promoter - ECFP D104 (BV) - D130 (BI) August 1 MP133 L112 in [http://partsregistry.org/Part:J61002 J61002] Weak promoter - GFP tripart D104 (BV) - D131 (BI) August 1 MP134 L115 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] pLas - ECFP D105 (BV) - D130 (BI) August 1 MP135 L116 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] pLas - gfp Tripart D105 (BV) - D131 (BI) August 1 MP136 L117 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] yfp - Double Terminator D125 (FV) - D117 (FI) August 1 MP137 L118 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] rfp - Double Terminator D125 (FV) - D121 (FI) August 1 MP138 L119 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] lasR activator + LVA - Double Terminator D125 (FV) - D113 (FI) August 1 MP139 L121 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] pTet - gfp tripart D106 (BV) - D131 (BI) August 1 MP140 L124 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strongest RBS - rfp D102 (BV) - D122 (BI) August 1 MP141 L125 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strongest RBS - yfp D102 (BV) - D118 (BI) August 1 MP142 [http://partsregistry.org/Part:pSB3K3 pSB3K3] August 8 MP143.1 [http://partsregistry.org/Part:BBa_E0240 E0240] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] gfp generator 138 August 8 MP143.2 150 MP144.1 L128 in [http://partsregistry.org/Part:J61002 J61002] pFlgA-RFP D132 (FI) - D136 (FV) MP144.3 ; MP144.4 127 August 12 MP144.2 166 MP144.3 163 MP144.4 154 MP145.1 L129 in [http://partsregistry.org/Part:J61002 J61002] pFlgB-RFP D133 (FI) - D136 (FV) MP145.6 ; MP145.7 136 August 12 MP145.2 151 MP145.6 163 MP145.7 188 MP146.1 L130 in [http://partsregistry.org/Part:J61002 J61002] pFlhB-RFP D134 (FI) - D136 (FV) MP146.1 ; MP146.2 298 August 12 MP146.2 142 MP146.7 170 MP146.8 143 MP147 L100 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] rbs TetR - ECFP D110 (BV) - D130 (BI) August 13 MP148.1 L101 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] rbs TetR - GFP tripart D110 (BV) - D131 (BI) DO NOT WORK !!!! MP148.2 ; MP148.3 109 August 13 MP148.2 123 MP148.3 123 MP149 L114 in [http://partsregistry.org/Part:pSB2K3 pSB2K3] AracpBAD - gfp tripart D126 (BV) - D131 (BI) August 13 MP150 L120 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] tetR repressible promoter - ECFP D106 (BV) - D130 (BI) August 13 MP151 L122 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] RBS-lasI - ECFP D107 (BV) - D130 (BI) August 13 MP152 L123 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] RBS lasI - gfp tripart D107 (BV) - D131 (BI) August 13 MP153.1 L126 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strongest RBS (1)- LasR activator (+LVA) D102 (BV) - D114 (BI) MP153.1 ; MP153.3 100 August 13 MP153.2 131 MP153.3 113 MP154 L132 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] flhDC D142(FV) - D139(FI) August 13 MP155 L133 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] OmpR* D142(FV) - D140(FI) WT sequence (August 19) August 13 ; August 19 MP156 L134 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] EnvZ* D142(FV) - D141(FI) lacks about 300 bp in EnvZ* (August 19) August 13 August 19 MP157 L138 in [http://partsregistry.org/Part:pSB3K3 pSB3K3] gfp generator (E0240) D137(FV) - D138(FI) August 13 MP158 L139 in [http://partsregistry.org/Part:pSB3K3 pSB3K3] E0240 RBS+ D140 (PstI) - D144 (ES) seems to have the plasmid without the insert and one with the insert August 15 MP159 L141 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] flhD D142 (ES) - D145 (ES) has not been sequenced August 15 MP160 L142 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] flhDC+promotor D143 (ES) - D145 (ES) does not have the insert August 15 MP161 L150 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] pLas + rbs-tetR-gfp tripart D105 (SP) - D146 (XP) incorrect clone -> to do again August 19 MP162.1 L151.1 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] rbs-lasR + double terminator D147 (ES) - D125 (EX) 151 August 19 MP162.2 172 MP162.3 149 MP163.1 [http://partsregistry.org/Part:BBa_B0032 B0032] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] RBS 80 August 20 MP163.2 79 MP164 [http://partsregistry.org/Part:BBa_E0422 E0422] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] RBS+ ECFP+ LVA+ term August 20 MP165 [http://partsregistry.org/Part:BBa_E0430 E0430] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] RBS+ YFP+ LVA- term August 20 MP166 [http://partsregistry.org/Part:BBa_E0432 E0432] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] RBS+ YFP LVA+ term BUT be careful , it doesn't work. See registry August 20 MP167 [http://partsregistry.org/Part:BBa_E0420 E0420] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] RBS+ ECFP LVA- term ceci ne contient pas de LVA apparemment. ça correspondrait au ECFP normal de longue durée de vie August 20 MP168 [http://partsregistry.org/Part:BBa_I732078 I732078] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] RBS+ mRFP LVA+ term August 20 MP171 L167 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] gfp generator(E0240) - pTet gfp generator(E0240) - pTet August 25 MP172 L166 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] RBS(B0032) - tetR RBS(B0032) - tetR August 25 Digestion Products (Legend: Vector, Insert) Ø Digestion Products Number Product Name Matrix Name Enzyme 1 Enzyme 2 Obs Size Matrix Size BB Description Product in use []ng/µL Date D100.1 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 34 10 July 26 D100.2 D101 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2076 15 10 July 29 D102.1 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2077 14 10 July 26 August 15 D102.2 D103.1 [http://partsregistry.org/Part:BBa_J23101 J23101] [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV 2100 883 27 July 26 D103.2 35 D104.1 [http://partsregistry.org/Part:BBa_J23109 J23109] [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV 2100 883 20 July 26 D104.2 D105.1 [http://partsregistry.org/Part:BBa_R0079 R0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2222 14 5 July 26 D105.2 D106.1 [http://partsregistry.org/Part:BBa_R0040 R0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2119 14 8 July 26 D106.2 D107 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2750 14 August 23 5 12 July 26 July 23 D108.1 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 707 5 July 29 D108.2 D109 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2056 708 July 26 10 July 26 D110 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2768 14 August 23 20 8 19 July 26 August 23 August 28 D111.1 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 725 20 July 26 D111.2 D112 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2079 703 8 September 5 D113 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] EcoRI SpeI FI 4402 779 20 July 26 D114 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] XbaI PstI BI 4003 778 20 July 26 D115.1 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 4402 746 - July 29 D115.2 D116.1 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 745 20 July 29 D116.1 D117 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI SpeI FI 3166 746 5 July 29 D118 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB2K3 pSB2K3] XbaI PstI BI 3167 745 20 July 26 D119 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2056 743 10 July 26 D120 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 742 10 July 26 D121 [http://partsregistry.org/Part:BBa_E1010 E1010] [http://partsregistry.org/Part:pSB2K3 pSB2K3] EcoRI SpeI FI 4402 704 10 July 26 D122 [http://partsregistry.org/Part:BBa_E1010 E1010] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 703 10 July 26 D123.1 [http://partsregistry.org/Part:BBa_J23100 J23100] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 26 July 26 D123.2 23 D124.1 [http://partsregistry.org/Part:BBa_J23107 J23107] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 15 July 26 D124.2 D125.1 [http://partsregistry.org/Part:BBa_B0015 B0015] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI XbaI FV 3303 15 26 10 July 26 August 23 August 28 D125.2 24 3687 D126.1 [http://partsregistry.org/Part:BBa_I0500 I0500] [http://partsregistry.org/Part:pSB2K3 pSB2K3] SpeI PstI BV 5621 14 10 July 26 D126.2 D127.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 37 10 July 26 D127.2 D128.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2079 15 10 July 29 D128.1 D129.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2080 14 10 July 29 D129.2 D130.1 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 939 10 July 26 D130.2 D130.3 D131.1 [http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 900 8 July 26 D131.2 D131.3 D132 pFlgA - EcoRI SpeI FI - 250 5 August 6 D133 pFlgB - EcoRI SpeI FI - 250 5 August 6 D134 pFlhB - EcoRI SpeI FI - 249 5 August 6 D135 pFlhDC - EcoRI SpeI FI - problem August 6 D136 - [http://partsregistry.org/Part:BBa_J61002 J61002] EcoRI SpeI FV 2925 - August 6 August 16 D137 pSB3K3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] EcoRI SpeI FV 2727 - August 6 D138 E0240 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 899 - August 6 D139 flhDC - EcoRI PstI FI - 972 August 6 D140 E040 rbs+ - PstI PstI I - August 12 D141 flhD RBS - - EcoRI SpeI FI - August 12 D142 flhC RBS - - EcoRI SpeI FI - August 12 D143 flhDC + promoter - EcoRI SpeI FI - August 12 D144 pSB3K3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] EcoRI SpeI FI - August 12 D145 pSB1A2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI - August 12 August 15 D146 MP148 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI 2057 1609 rbs TetR - GFP tripart D110 (BV) - D131 (BI) August 15 D147 MP153 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 2056 799 Strongest RBS (1)- LasR activator (+LVA) D102 (BV) - D114 (BI) August 15 D149 pfliL - EcoRI SpeI FI - August 14 August 16 D150 pfliL - XbaI PstI BI - August 14 August 16 D152 pSB3K3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] XbaI PstI BI - August 14 D153 PCR 138 - gène flhDC - EcoRI SpeI FI - August 15 D154 PCR 140 - gène flhDC - EcoRI SpeI FI - August 15 D155 PCR 139 - pflhDC (URI) - EcoRI SpeI FI - August 15 D158 MP 155.1 - OmpR* [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI - August 15 D159 MP 156.1 - EnvZ* [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI - August 15 D161 MP 104 - pTet [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV - 39 August 16 D162 MP 114 - TetR [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI - 11 August 16 D163 MP 143 - gfp generator [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI - August 23 14 11 August 16 August 23 D164 MP 101 - pJ23101 [http://partsregistry.org/Part:BBa_pJ61002 pJ61002] SpeI PstI BV - 3 August 16 D165 MP162.2 [http://partsregistry.org/Part:BBa_pSB1AK3 pSB1AK3] XbaI PstI BI 2057 798 rbs-lasR - double terminator D147 (FI) - D125 (FV) 27 August 18 D166 MP 165.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV - 2942 bp RBS (B0034)+ YFP+ LVA- term B0015 59 August 26 September 3 D179 MP3.4 [http://partsregistry.org/Part:BBa_pSB1AK3 pSB1AK3] SpeI PstI BV B0015 (double terminator B0010-B0012) August 20 D180 MP101.1 [http://partsregistry.org/Part:BBa_pJ61002 pJ61002] SpeI PstI BV promoter J23101 August 20 D181 MP104.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV pTet (TetR repressible promoter) August 20 D182 MP114.1 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI TetR August 20 D183 MP119.3 - XbaI PstI BI pBad promoter August 20 D184 MP143.1 [http://partsregistry.org/Part:BBa_pSB2K3 pSB2K3] EcoRI SpeI FI gfp generator August 18 D185 MP163.1 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV B0032 RBS August 18 D187 MP168.1 [http://partsregistry.org/Part:BBa_pSB1A3 pSB1A3] EcoRI XbaI FV RBS (B0034)+ mRFP LVA+ term B0015 43 September 3 D188 PCR 138 - XbaI PstI Insert - 767 bp OmpR* August 26 D189 MP 101.2 [http://partsregistry.org/Part:BBa_pJ61002 pJ61002] XbaI PstI Vector - 2073 bp pSB1A2 August 26 D191 MP114.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV 2724 tetR 22 August 29 D192 MP144.2 [http://partsregistry.org/Part:BBa_pJ61002 pJ61002] EcoRI XbaI FV 2267 FlgA 26 August 29 D193 MP171 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 961 gfp generator(E0240) - pTet 9,5 August 29 D194 MP172.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 704 RBS(B0032) - tetR 13 August 29 D195 MP172.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI 703 RBS(B0032) - tetR 11 August 29 D196 MP104.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV 2119 pTet 45 September 3 D197 MP157.2 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] XbaI PstI BI 898 gfp generator(E0240) 12 September 3 D198 MP164.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV 2981 RBS (B0034)+ ECFP+ LVA+ term B0015 36 September 3 D199 MP167.1 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV 2942 RBS (B0034)+ ECFP LVA- term (B0015) 61 September 3 D200 MP171.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI 960 gfp generator(E0240) - pTet 43 September 3 D201 MP114.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV 2724 tetR 13 August 29 Strains (glycerol stock) Strains glycerol stock Number Name Biobricks Description Verified clone Date S103 [http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3] AraC pBAD July 23 S106 [http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] ECFP (RBS+LVA+ Double Term) July 23 S107 [http://partsregistry.org/Part:BBa_B0034 B0034] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strongest RBS (Efficiency = 1) July 23 S108 [http://partsregistry.org/Part:BBa_R0079 R0079] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] promoter pLas (lasr & PAI regulated) July 23 S109 [http://partsregistry.org/Part:BBa_J23101 J23101] in [http://partsregistry.org/Part:BBa_J61002 pJ61002 ] Strongest Constitutive promoter July 23 S110 [http://partsregistry.org/Part:BBa_J23109 J23109] in [http://partsregistry.org/Part:BBa_J61002 pJ61002 ] Weak Constitutive promoter July 23 S111 [http://partsregistry.org/Part:BBa_R0040 R0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] tetR repressible promoter July 23 S112 [http://partsregistry.org/Part:BBa_S03154 S03154] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] B0034(rbs) ->LasI July 23 S113 [http://partsregistry.org/Part:BBa_S03879 S03879] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] B0034(rbs) -> TetR July 23 S114 [http://partsregistry.org/Part:BBa_C0079 C0079] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] lasR activator with LVA Tag July 23 S115 [http://partsregistry.org/Part:BBa_C0179 C0179] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] lasR activator July 23 S116 [http://partsregistry.org/Part:BBa_E0030 E0030] in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] YFP July 23 S117 [http://partsregistry.org/Part:BBa_E0040 E0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] GFP July 23 S118 [http://partsregistry.org/Part:BBa_E1010 E1010] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] mRFP July 23 S119 OmpR M57I July 23 S120 EnvZ I281S July 23 S121 [http://partsregistry.org/Part:BBa_C0040 C0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] tetR repressor July 23 S122 [http://partsregistry.org/Part:pSB1A3 pSB1A3] July 23 S123 L102 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strong rbs - YFP D129 (BV) - D118 (BI) Aug 1 S124 L103 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strong rbs - mRFP D129 (BV) - D122 (BI) Aug 1 S125 L104 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strong rbs - lasR activator D129 (BV) - D114 (BI) Aug 1 S126 L105 in [http://partsregistry.org/Part:J61002 J61002] Strong promoter - ECFP D123 (BV) - D130 (BI) Aug 1 S127 L106 in [http://partsregistry.org/Part:J61002 J61002] Strong promoter - gfp Tripart D123 (BV) - D131 (BI) Aug 1 S128 L107 in [http://partsregistry.org/Part:J61002 J61002] Strongest promoter - ECFP D103 (BV) - D130 (BI) Aug 1 S129 L108 in [http://partsregistry.org/Part:J61002 J61002] Strong promoter - GFP tripart D103 (BV) - D131 (BI) Aug 1 S130 L110 in [http://partsregistry.org/Part:J61002 J61002] Medium promoter - gfp tripart D124 (BV) - D131 (BI) Aug 1 S131 L111 in [http://partsregistry.org/Part:J61002 J61002] Weak promoter - ECFP D104 (BV) - D130 (BI) Aug 1 S132 L112 in [http://partsregistry.org/Part:J61002 J61002] Weak promoter - gfp Tripart D104 (BV) - D131 (BI) Aug 1 S133 L115 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] pLas - ECFP D105 (BV) - D130 (BI) Aug 1 S134 L116 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] pLas - gfp Tripart D105 (BV) - D131 (BI) Aug 1 S135 L117 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] yfp - Double Terminator D125 (FV) - D117 (FI) Aug 1 S136 L118 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] rfp - Double Terminator D125 (FV) - D121 (FI) Aug 1 S137 L119 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] LasR activator + LVA - Double terminator D125 (FV) - D113 (FI) Aug 1 S138 L121 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] pTet - gfp Tripart D106 (BV) - D131 (BI) Aug 1 S139 L124 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strongest rbs - mRFP D102 (BV) - D122 (BI) Aug 1 S140 L125 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strongest rbs - yfp D102 (BV) - D118 (BI) Aug 1 S141 MG1655 Aug 7 S142 [http://partsregistry.org/Part:J61002 J61002] Aug 7 S143 L128 in [http://partsregistry.org/Part:J61002 J61002] pFlgA-RFP D132 (FI) - D136 (FV) MP144.3 ; MP144.4 August 12 S144 L129 in [http://partsregistry.org/Part:J61002 J61002] pFlgB-RFP D133 (FI) - D136 (FV) MP145.6 ; MP145.7 August 12 S145 L130 in [http://partsregistry.org/Part:J61002 J61002] pFlhB-RFP D134 (FI) - D136 (FV) MP146.1 ; MP146.2 August 12 S146 L100 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] rbs TetR - ECFP D110 (BV) - D130 (BI) August 13 S147 L101 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] rbs TetR - GFP tripart D110 (BV) - D131 (BI) August 13 S148 L114 in [http://partsregistry.org/Part:pSB2K3 pSB2K3] AracpBAD - gfp tripart D126 (BV) - D131 (BI) August 13 S149 L120 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] tetR repressible promoter - ECFP D106 (BV) - D130 (BI) August 13 S150 L122 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] RBS-lasI - ECFP D107 (BV) - D130 (BI) August 13 S151 L123 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] RBS lasI - gfp tripart D107 (BV) - D131 (BI) August 13 S152 L126 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strongest RBS (1)- LasR activator (+LVA) D102 (BV) - D114 (BI) August 13 S153 L132 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] flhDC D142(FV) - D139(FI) August 13 S154 L133 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] OmpR* D142(FV) - D140(FI) August 13 S154 L133.1 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] OmpR* D142(FV) - D140(FI) WT sequence August 19 S155 L134 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] EnvZ* D142(FV) - D141(FI) August 13 S155 L134.2 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] EnvZ* D142(FV) - D141(FI) lacks about 300 bp in EnvZ* August 19 S156 L138 in [http://partsregistry.org/Part:pSB3K3 pSB3K3] gfp generator (E0240) D137(FV) - D138(FI) August 13 S157 E0240 GFP generator August 15 S158 pSB3K3 plasmid August 15 S159.1 L139.1 in in [http://partsregistry.org/Part:pSB3K3 pSB3K3] E0240 RBS+ D140 (PstI) - D144 (ES) seems to have the plasmid without the insert and one with the insert August 15 S159.2 incorrect clone S160.1 L141.1 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] flhD D142 (ES) - D145 (ES) has not been sequenced August 15 S160.2 has not been sequenced S160.3 has not been sequenced S161.1 L142.7 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] flhDC+promotor D143 (ES) - D145 (ES) does not seem to have the insert August 15 S161.2 incorrect clone S162 MP162.1 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] rbs-lasR + double terminator D147 (ES) - D125 (EX) August 18 S163 B0032 in pSB1A2 medium rbs (efficiency = 0,3) August 20 S164 E0422 strongest rbs ECFP LVA+ double terminator August 20 S165 E0430 strongest rbs YFP LVA- double terminator August 20 S166 E0432 strongest rbs YFP LVA+ double terminator August 20 S167 E0420 strongest rbs ECFP LVA- double terminator August 20 S168 I732078 strongest rbs mRFP LVA+ double terminator August 20

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