Team:KULeuven/30 August 2008

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• The Team
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  Summary Components

  Input Output Filter InverTimer Reset Cell Death Memory

  Safety End Evaluation Literature Brainstorm
• Ethics
  Introduction Three A's

  Defining the three A's Developments in modern physics Developments in biology

  Concerns & Issues

  Defining synthetic biology A framework Concerns Issues The need for answers

  Biological Robotics

  KULeuven iGEM 2008 project Biological robotics

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• Modeling
  Overview Kinetic Constants Components

  Output Filter InverTimer Reset Cell Death Memory

  Full Model Sensitivity Analysis Multi-cell Model Diffusion
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  GFP (LVA-tag) Other

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Lab Work

Wet Lab

• We MiniPrepped the new parts and the two ligations that succeeded (C0040+B0015 and C0060+B0015).
• The digestions with XbaI were started.
• The ligations we made yesterday were electroporated today (still in electrocompetent Top10 cells).
• A liquid culture was made of part R0053.

Dry Lab

Created the oligos for the B1002 and B1006 terminators [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=terminator link]

Modeling

Final updated model uploaded to the wiki (only MatLab)

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