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Team:Hawaii/Notebook/2008-09-30
From 2008.igem.org
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Plasmid Prep
- Grace
- Plasmid prep of BBpRL1383a #1, 3, 13, 18
Construction of secretion device (cont.)
- Grace
- PCR:
- nir+rbs
- slr1+GFPf
- plac+rbs
- rbs+GFP
- nir
- nir+rbs+pilA #17 (yet to be confirmed via sequencing)
- rbs+GFPf+tt #1, 2 (yet to be confirmed via sequencing)
- GFPf+tt #21
- RE digest:
- EcoRI/PstI:
- pSB1A3
- EcoRI/SpeI:
- nir
- rbs+GFP
- nir+rbs
- plac+rbs
- nir+rbs+pilA #17
- XbaI/PstI:
- slr1+GFPf
- rbs+GFPf+tt #1, 2
- GFPf+tt #21
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
