Team:Chiba/Calendar-Home/7 September 2008

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6 September 2008 <|> 7 September 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

-->(6/9) Digestion Test

Sample DNA 1 : BBa_R0010(unknown)[http://partsregistry.org/Part:BBa_R0010]
Sample DNA 2 : BBa_R0010(100ng/μL)[http://partsregistry.org/Part:BBa_R0010]
Sample No 1,2
Sample DNA 12
PstⅠ 0.5
SpeⅠ 0.5
Buffer 2 2
(×10)BSA 2
dH2O 3
TOTAL 20


--->Gel Check
Sample leftright
Sample DNA 55
Loading Dye 11
TOTAL 66

Left side:BBa_R0010 Sample No.1
Right side:BBa_R0010 Sample No.2

-->(8/9)


Team:Output

  • Cloning

Digestion

  • vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)① 75ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)② 33.6ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)③ 33.6ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](RBS+cinR)④ 38.4ng/μl
  • vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](pLac)⑤ 100ng/μl
  • insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]⑥ 33.6ng/μl
Sample No. ①②③④⑤⑥
DNA(digested sample) 505454451454
SpeⅠ 100010
PstⅠ 0.50.50.50.50.50.5
XbaⅠ 00.50.50.500.5
BSA(x10) 0.677627
Buffer2 6---2-
Buffer3 -776-7
dH2O 1.91120.51
TOTAL 607070602070

-->37℃ 3hour

-->Gel Extraction

Sample No. ①②③④⑤⑥
DNA(digested sample) 6101010410
Loading Dye 222212
dH2O 4---12
TOTAL 12121212612
well 1077657

-->DNA purification (①-④,⑥:7μl ⑤:6μl)

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