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User contributions
From 2008.igem.org
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- 20:35, 30 July 2008 (diff | hist) N Virginia/30 July 2008 (New page: ==Overnight Results== *No colonies ==Accomplished== *A42-A45 **Three RBS+BP assemblies have terminator attached **A45 is E0040 + B0015)
- 14:17, 30 July 2008 (diff | hist) Virginia/29 July 2008 (top)
- 18:32, 29 July 2008 (diff | hist) m Team:Virginia/Sandbox
- 18:17, 29 July 2008 (diff | hist) m Team:Virginia/Sandbox
- 18:06, 29 July 2008 (diff | hist) m Team:Virginia/Sandbox
- 18:05, 29 July 2008 (diff | hist) m Team:Virginia/Sandbox
- 18:03, 29 July 2008 (diff | hist) N Team:Virginia/Sandbox (New page: 500px <br> *Home *Team *Project *Modeling *Parts *Notebook)
- 14:57, 29 July 2008 (diff | hist) N Virginia/29 July 2008 (New page: ==Overnight Results== *Although plates have only grown ~13h most have colonies **A37 and A38 don't have colonies. They both used 1AK3)
- 20:17, 28 July 2008 (diff | hist) N Virginia/28 July 2008 (New page: ==Accomplished== *Assemblies: 32-41 **Putting constitutive promoters on BP assemblies (A32-A34) **I715039 + E0840 (A36) **RBS + A29 (A37) **A1 + E0040 (A38) **RBS + BP1-3 (A39-A41) *Transf...) (top)
- 19:14, 25 July 2008 (diff | hist) N Virginia/25 July 2008 (New page: ==Overnight Results== *EVERYTHING WORKED! all ligations were successful thanks to the new T4 ligase *Also the E0840 plate from the day before now had colonies ==Accomplished== *Made smal...) (top)
- 19:35, 24 July 2008 (diff | hist) N Virginia/23 July 2008 (New page: ==Accomplished== *Eluted parts **E0840 **I732097 **E1010 *Digestion - only 3h instead of 4h. Don't want non-specific restriction. **A1 **A25-A30 *Gelled all restriction digests **Cut some ...) (top)
- 19:23, 24 July 2008 (diff | hist) N Virginia/22 July 2008 (New page: ==Accomplished== *SDS-PAGE gel of induced BP1 expression **No apparent new protein expression *Determined new course for assemblies *Sent construction site sequences to be ordered [[Team...) (top)
- 15:48, 24 July 2008 (diff | hist) m Virginia/24 July 2008 (top)
- 15:48, 24 July 2008 (diff | hist) m Virginia/24 July 2008
- 15:47, 24 July 2008 (diff | hist) N Virginia/24 July 2008 (New page: ==Overnight Results== *Parts from distribution did not grow - no colonies ==Goals== *Do assemblies with new T4 DNA ligase **20 ul mix *Transform and plate assemblies *Order Placeholder si...)
- 18:33, 23 July 2008 (diff | hist) Team:Virginia/Protocols
- 13:19, 21 July 2008 (diff | hist) N Virginia/19 July 2008 (New page: *Spun down pellets of overnight broths *Plates with A14 - A17 didn't have results)
- 16:04, 16 July 2008 (diff | hist) m Virginia/16 July 2008 (→Goals) (top)
- 15:12, 16 July 2008 (diff | hist) N Virginia/16 July 2008 (New page: ==Overnight Results== *Plates didn't have anything on them. We plated them late (8pm) so we'll let them keep growing <br> ==Goals== *Run gel of pSB1A10, A3, A$, BP2, BP3 *Redo assembly of:...)
- 20:26, 10 July 2008 (diff | hist) m Virginia/10 July 2008 (top)
- 20:25, 10 July 2008 (diff | hist) N Virginia/10 July 2008 (New page: ==Overnight Results== *Assembly 4 (RBS + BP1) had colonies *Assembly 3 (I715039 + E0240) didn't have colonies ==Goals== *Make small (10ml) broth of colonies in Assembly 4 plate *Redo dige...)
- 17:07, 9 July 2008 (diff | hist) Virginia/9 July 2008 (top)
- 13:59, 9 July 2008 (diff | hist) N Virginia/9 July 2008 (New page: ==Overnight Results== *pSB1A10 screening plasmid grew just fine *Assembly 1 (BP1 + Term) grew more colonies *pSB1AC3 and pSB1AK3 both grew a small number of colonies <br>)
- 22:19, 8 July 2008 (diff | hist) Virginia/8 July 2008 (top)
- 02:00, 2 July 2008 (diff | hist) m Team:Virginia (Undo revision 11161 by DRTarjan (Talk))
- 01:53, 2 July 2008 (diff | hist) Team:Virginia
- 00:55, 2 July 2008 (diff | hist) m Team:Virginia/Notebook
- 00:46, 2 July 2008 (diff | hist) m Team:Virginia/Project
- 00:44, 2 July 2008 (diff | hist) Team:Virginia/Project (added draft project descriptions)
- 01:57, 1 July 2008 (diff | hist) Virginia/30 June 2008 (top)
- 01:54, 1 July 2008 (diff | hist) Virginia/6 June 2008 (Removing all content from page) (top)
- 20:50, 30 June 2008 (diff | hist) Team:Virginia/Notebook (changed style and such)
- 03:36, 30 June 2008 (diff | hist) Team:Virginia/Team (added nicknames) (top)
- 03:18, 30 June 2008 (diff | hist) m Team:Virginia/Team (added <p>'s)
- 03:15, 30 June 2008 (diff | hist) Team:Virginia/Team (changed to use a div for each person)
- 15:45, 25 June 2008 (diff | hist) Team:Virginia/Team (added pictures)
- 15:44, 25 June 2008 (diff | hist) N File:Dan.JPG (top)
- 15:40, 25 June 2008 (diff | hist) N File:Georgew.jpg (top)
- 15:40, 25 June 2008 (diff | hist) N File:Patrik.jpg (top)
- 15:40, 25 June 2008 (diff | hist) N File:Brandon.JPG (top)
- 15:40, 25 June 2008 (diff | hist) N File:Eyad.JPG (top)
- 15:39, 25 June 2008 (diff | hist) m Team:Virginia
- 15:39, 25 June 2008 (diff | hist) Team:Virginia (Undo revision 8648 by DRTarjan (Talk))
- 15:38, 25 June 2008 (diff | hist) m Team:Virginia
- 15:37, 25 June 2008 (diff | hist) Team:Virginia/Project (added picture)
- 15:36, 25 June 2008 (diff | hist) N File:Board.JPG (VGEM's whiteboard) (top)
- 15:35, 25 June 2008 (diff | hist) Team:Virginia (added team picture)
- 15:33, 25 June 2008 (diff | hist) N File:Team.jpg (VGEM Team picture) (top)
- 15:15, 25 June 2008 (diff | hist) Team:Virginia/Modeling (top)
- 15:14, 25 June 2008 (diff | hist) Team:Virginia/Parts (changed to html based design)
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