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User contributions
From 2008.igem.org
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- 19:10, 11 August 2008 (diff | hist) N Team:Mississippi State/29 July 2008 (New page: # Prepare low salt LB plates w/ 50ug/ml BLAST # Prepare blast stock solution, 50mg + 1ml # make new LB plates # make YPD plates # prepare ligation mix using T4 Dna Ligase and LiP and pPIC6...) (top)
- 19:07, 11 August 2008 (diff | hist) N Team:Mississippi State/28 July 2008 (New page: # prepare 500uM stock solution of primers # prepare working stock of primers # prepare PCR mix: 40ul ddH2O, 5ul PfxBuffer, 1ul dNTP, 1ul Up2, 1ul Down2, 1ul RP#1, 1ul Pfx50 # Make low salt...) (top)
- 19:02, 11 August 2008 (diff | hist) N Team:Mississippi State/23 July 2008 (New page: # sequence was satisfactory # designed new primers, ordered: Upstream2, Downstream2, Sequence # Prepare and run 1% agarose gel to test conc. of pPIC6alpha vector stock. # grow more cells f...) (top)
- 19:00, 11 August 2008 (diff | hist) N Team:Mississippi State/22 July 2008 (New page: # prepare sequencer mix. DON'T USE SP6 AND T7 FOR PCR PRODUCTS!! # perform previous work for sequencing) (top)
- 18:59, 11 August 2008 (diff | hist) N Team:Mississippi State/21 July 2008 (New page: # Sequencing results negative for LiP # Check O.D. of RP and BKM PCR products # prepare PCR mix(RP then BKM): TRR(4,4), Template(1,1.5), primer(1,1), 5xBuffer(2,2), ddH2O(12,11.5) # after ...) (top)
- 18:56, 11 August 2008 (diff | hist) N Team:Mississippi State/18 July 2008 (New page: # Transfer PCR product to 1.5ml microtube w/ 2ul 3M NaAc and 50ul 95%etOH; mix and ice 15min; centri 10min, discard super; add 250ul 70%etOH, centri 5min, dicard super; vacuum dry, resuspe...) (top)
- 18:53, 11 August 2008 (diff | hist) N Team:Mississippi State/17 July 2008 (New page: # Extract plasmid using P1,P2,P3 buffers # Prepare and run 1% agarose gel # Check O.D. for samples: BKM#1 and BKM#6 # Make sequencing mix(x2): 4ul TRR, 1ul Template, 1ul Sp6(#1) 1ul T7(#2)...) (top)
- 18:50, 11 August 2008 (diff | hist) N Team:Mississippi State/16 July 2008 (New page: # Pick colonies(5 white, 1 blue), grow in 2ml LB + Amp ~10 hrs @37C) (top)
- 18:48, 11 August 2008 (diff | hist) N Team:Mississippi State/15 July 2008 (New page: # Inoculate cells # Prepare amp plates # Make competent cells # perform transformation w/ pGEM ligation mix) (top)
- 18:46, 11 August 2008 (diff | hist) N Team:Mississippi State/14 July 2008 (New page: # Pick colony from plates and prepare 2ml LB culture + Amp # grow @37C ~10hrs # transfer 1.5ml to microtube, centrifuge 5 min. # extract plasmid using QIAgen kit # prepare and run 1% agaro...) (top)
- 18:43, 11 August 2008 (diff | hist) N Team:Mississippi State/11 July 2008 (New page: # Inoculate cells: 25ml LB + 100ul Tet + 250ul XLI-Blue overnight culture in 250ml flask in 37C 1hr. # Prepare competent cells # Perform transformation of pGEM ligation mix. # incubate 37C...) (top)
- 18:40, 11 August 2008 (diff | hist) N Team:Mississippi State/10 July 2008 (New page: # Run 1% agarose gel w/ 3x10ul of each sample from PCR # cut bands, right sample= #1(RP), left sample = #2(BKM) # Purify gel using QIAgen Kit # Prepare and run 1% agarose gel w/ 2ul each s...) (top)
- 18:38, 11 August 2008 (diff | hist) N Team:Mississippi State/9 July 2008 (New page: # Make 500uM stock solution of Upstream and Downstream primers. # Make working solution of primers: 1ul stock + 49ul ddH2O # Prepare PCR mix (for each sample, BKM and RP): 39ul ddH2O + 5ul...) (top)
- 18:33, 11 August 2008 (diff | hist) Team:Mississippi State/Papers (→Notebook)
- 18:33, 11 August 2008 (diff | hist) Team:Mississippi State/Papers (→Notebook)
- 18:32, 11 August 2008 (diff | hist) Team:Mississippi State/Papers (→Notebook)
- 18:31, 11 August 2008 (diff | hist) Team:Mississippi State/Papers (→Notebook)
- 14:00, 31 July 2008 (diff | hist) Team:Mississippi State
- 13:59, 31 July 2008 (diff | hist) Team:Mississippi State/So Far (→Mississippi State University Synthetic Biology) (top)
- 13:58, 31 July 2008 (diff | hist) Team:Mississippi State
- 13:57, 31 July 2008 (diff | hist) Team:Mississippi State
- 13:57, 31 July 2008 (diff | hist) Team:Mississippi State
- 13:57, 31 July 2008 (diff | hist) Team:Mississippi State
- 13:56, 31 July 2008 (diff | hist) Team:Mississippi State
- 13:55, 31 July 2008 (diff | hist) Team:Mississippi State/Papers
- 13:54, 31 July 2008 (diff | hist) Team:Mississippi State/Project
- 13:53, 31 July 2008 (diff | hist) Team:Mississippi State/Project
- 13:50, 31 July 2008 (diff | hist) Team:Mississippi State/Project
- 13:50, 31 July 2008 (diff | hist) Team:Mississippi State/Project
- 13:49, 31 July 2008 (diff | hist) Team:Mississippi State/Project
- 13:49, 31 July 2008 (diff | hist) Team:Mississippi State/Project
- 13:48, 31 July 2008 (diff | hist) Team:Mississippi State/Project
- 13:46, 31 July 2008 (diff | hist) Team:Mississippi State/Project
- 13:46, 31 July 2008 (diff | hist) Team:Mississippi State/Team
- 13:45, 31 July 2008 (diff | hist) Team:Mississippi State/caleb (top)
- 13:44, 31 July 2008 (diff | hist) Team:Mississippi State/caleb
- 13:43, 31 July 2008 (diff | hist) Team:Mississippi State
- 13:42, 31 July 2008 (diff | hist) Team:Mississippi State
- 16:31, 16 July 2008 (diff | hist) File:TheRealProblem.JPG (uploaded a new version of "Image:TheRealProblem.JPG") (top)
- 16:26, 16 July 2008 (diff | hist) Team:Mississippi State/caleb
- 16:26, 16 July 2008 (diff | hist) Team:Mississippi State/caleb
- 16:25, 16 July 2008 (diff | hist) N File:TheRealProblem.JPG
- 15:45, 16 July 2008 (diff | hist) Team:Mississippi State/Project (→Overall project)
- 15:38, 16 July 2008 (diff | hist) Team:Mississippi State/Project (→Overall project)
- 15:36, 16 July 2008 (diff | hist) Team:Mississippi State/Project (→Overall project)
- 13:55, 15 July 2008 (diff | hist) Team:Mississippi State/madison (top)
- 13:54, 15 July 2008 (diff | hist) N Team:Mississippi State/madison (New page: <SCRIPT LANGUAGE="JavaScript"> <!-- floatX=0; floatY=110; layerwidth=155; layerheight=130; halign="left"; valign="top"; delayspeed=1; // This script is copyright (c) Henrik Petersen, NetK...)
- 13:29, 11 July 2008 (diff | hist) Team:Mississippi State/Project
- 13:28, 11 July 2008 (diff | hist) N File:Bandit2.JPG (top)
- 13:19, 11 July 2008 (diff | hist) File:Bandit.JPG (uploaded a new version of "Image:Bandit.JPG") (top)
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