"
User contributions
From 2008.igem.org
(Latest | Earliest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)
- 02:33, 24 September 2008 (diff | hist) m Team:Virginia/Sandbox
- 00:30, 24 September 2008 (diff | hist) Team:Virginia/Sandbox
- 00:22, 24 September 2008 (diff | hist) N File:Rsbp.gif (top)
- 00:22, 24 September 2008 (diff | hist) N File:Team.gif (top)
- 00:21, 24 September 2008 (diff | hist) N File:Notebook2.gif (top)
- 00:21, 24 September 2008 (diff | hist) N File:Ga.gif (top)
- 00:21, 24 September 2008 (diff | hist) N File:Home.gif (top)
- 00:20, 24 September 2008 (diff | hist) N File:Bp.gif (top)
- 00:20, 24 September 2008 (diff | hist) N File:Rotunda.JPG
- 00:19, 24 September 2008 (diff | hist) N File:Pixel-logo.jpg
- 21:23, 23 September 2008 (diff | hist) N Virginia/23 September 2008 (New page: ==Overnight Results== *A few small colonies were ==Accomplished== *Overnight broth of A73-75 **10ml LB with amp Back to Notebook) (top)
- 02:19, 23 September 2008 (diff | hist) Virginia/22 September 2008 (top)
- 20:38, 22 September 2008 (diff | hist) Virginia/22 September 2008
- 20:37, 22 September 2008 (diff | hist) Virginia/21 September 2008 (top)
- 15:48, 22 September 2008 (diff | hist) N Virginia/22 September 2008 (New page: *Melted cut gel parts - ~69C for 10min *Ligations for A73-75 **30min at room temp **6:1 volume ratio of melted gel of insert:vector)
- 15:45, 22 September 2008 (diff | hist) N Virginia/21 September 2008 (New page: *Ran R0040(s,p) through low melt gel *cut out R0040)
- 03:39, 19 September 2008 (diff | hist) N Virginia/18 September 2008 (New page: *Ran low-melt gel of A70-72(X,P) **100V, 1h45 *Cut out and put into fridge Back to Notebook) (top)
- 23:51, 18 September 2008 (diff | hist) N Virginia/17 September 2008 (New page: *Ran gel of A70-72 cut with X,P *Gel looks right **Means we should have RET versions of the 3 bioplastic genes Back to Notebook) (top)
- 14:53, 12 September 2008 (diff | hist) N Virginia/12 September 2008 (New page: *Miniprepped A70-71 Back to Notebook) (top)
- 04:22, 8 September 2008 (diff | hist) N Virginia/7 September 2008 (New page: *Cut parts for A70-A72 **(RBS+BP) assemblies were cut E,S **B0015 was cut E,X **>Standard assembly *Also, used old cuts of A58(E,S) and A59(E,X) to again create an (RBS+BP1) + (RBS+BP2) a...) (top)
- 04:21, 8 September 2008 (diff | hist) Virginia/6 September 2008 (Removing all content from page) (top)
- 04:21, 8 September 2008 (diff | hist) N Virginia/6 September 2008 (New page: *Cut parts for A70-A72 **(RBS+BP) assemblies were cut E,S **B0015 was cut E,X **>Standard assembly *Also, used old cuts of A58(E,S) and A59(E,X) to again create an (RBS+BP1) + (RBS+BP2) a...)
- 17:38, 4 September 2008 (diff | hist) N Virginia/3 September 2008 (New page: *Saw a mistake in the notebook, means last set of assemblies were mis-cut *Cut assemblies for (RBS+BP) + Term again correctly **Both 3A and standard *Made low-melt gel to purify parts [[...) (top)
- 04:51, 1 September 2008 (diff | hist) N Virginia/31 August 2008 (New page: *Miniprepped A67-A69 **Concentrations very good: ~200 or more ng/ul (almost suspiciously good) *Cut A67-A69 with S,P for assumed next stage of 3A *Made gel to look at cut fragments tomorro...) (top)
- 04:50, 1 September 2008 (diff | hist) N Virginia/30 August 2008 (New page: *Spun down A67-69 *Froze pellets Back to Notebook) (top)
- 19:23, 31 August 2008 (diff | hist) Team:Virginia/Notebook
- 18:47, 29 August 2008 (diff | hist) N Virginia/29 August 2008 (New page: ==Overnight Results== *All plates (A67-69) had several dozen colonies ==Accomplished== *Made fresh chloramphenicol antibiotic mix *Made small overnight broths of all 3 assemblies [[Team...) (top)
- 18:46, 29 August 2008 (diff | hist) N Virginia/28 August 2008 (New page: *Ligated (3A) parts for A67-69 **Heat inactivated @65C for 10min *Transformed 30ul of cells with 5ul of ligated DNA Back to Notebook) (top)
- 23:32, 27 August 2008 (diff | hist) N Virginia/27 August 2008 (New page: *Doing restrictions for assemblies of (RBS+BP) with a terminator **Using updated protocol RE: heat-inactivation Back to Notebook) (top)
- 06:00, 20 August 2008 (diff | hist) m Virginia/18 August 2008 (top)
- 06:00, 20 August 2008 (diff | hist) N Virginia/19 August 2008 (New page: ==Overnight Results== *Only plates for B1002 and A64 had some (very small) growth ==Accomplished== *Re-plated A61-63 *Made small overnight broth of B1002 and A64 [[Team:Virginia/Notebo...) (top)
- 22:59, 18 August 2008 (diff | hist) N Virginia/18 August 2008 (New page: *Gelled b0015 e0040 i715039 A59 A60 **Look good *Cut out the above and A58-60 *Doing ligations for A61-66)
- 17:13, 15 August 2008 (diff | hist) N Virginia/15 August 2008 (New page: *Gelled A58-A60 and B0034 Back to Notebook) (top)
- 21:22, 14 August 2008 (diff | hist) Virginia/14 August 2008 (top)
- 18:20, 14 August 2008 (diff | hist) N Virginia/14 August 2008 (New page: ==Overnight Results== *A58 re-plate had a few colonies *A59 re-plate had no colonies ==Accomplished== *Made small broth of A58 (2) *Miniprepped A59-A60, B0034 [[Team:Virginia/Notebook|B...)
- 19:45, 13 August 2008 (diff | hist) Virginia/13 August 2008 (top)
- 19:24, 13 August 2008 (diff | hist) m Virginia/13 August 2008
- 19:24, 13 August 2008 (diff | hist) Virginia/13 August 2008
- 18:08, 13 August 2008 (diff | hist) N Virginia/13 August 2008 (New page: *Made small broth of A58-A60 **Not sure about A58 and A59 - few colonies, satellites *Made small broth of re-streaked B0034 **Still not confident in this *Digested I715039 with E,S and B1...)
- 20:13, 12 August 2008 (diff | hist) N Virginia/12 August 2008 (New page: *Started butanonlysis **Leaving it overnight to make sure reaction goes to completion *Induced A51 in broth, hoping to see red *A58-A60 plates **Some have small colonies, we're letting the...) (top)
- 21:27, 11 August 2008 (diff | hist) N Virginia/11 August 2008 (New page: ==Accomplished== *Re-plate B0034 for the 3rd time (from leftover cells from 2nd transformation) *Small broth of A51 (RFP + GFP) so that we can then take the broth to a spectromoter *Digest...) (top)
- 14:50, 9 August 2008 (diff | hist) N Virginia/9 August 2008 (New page: ==Overnight Results== *No growth on plates except for positive control **Weren't really expecting any **B0034 didn't grow either though unfortunately ==Goals== *Re-transform B0034 and pla...) (top)
- 20:29, 8 August 2008 (diff | hist) Virginia/8 August 2008 (→Accomplished) (top)
- 16:42, 8 August 2008 (diff | hist) Virginia/8 August 2008
- 13:53, 8 August 2008 (diff | hist) N Virginia/8 August 2008 (New page: ==Overnight Results== *No colonies on plates. Back to Notebook)
- 13:52, 8 August 2008 (diff | hist) N Virginia/7 August 2008 (New page: ==Accomplished== *Looked at stained gel of 1st order assemblies (47-50). No inserts were visible. This suggests the assemblies are not correct. *Bought XbaI *Cut out B0034 (RBS) out of gel...) (top)
- 22:01, 30 July 2008 (diff | hist) m Virginia/31 July 2008 (top)
- 21:56, 30 July 2008 (diff | hist) m Virginia/31 July 2008
- 21:56, 30 July 2008 (diff | hist) N Virginia/31 July 2008 (New page: ==TODO== *Redo A1 (R0040 + B0034) *Redo A39 (B0034 + BP1) **Redo NanoDrop of A39 **Redo (if NanoDrop is bad) miniprep of A39 *Overnight broth of more E0840 colonies *NanoDrop 3 miniprepped...)
- 21:48, 30 July 2008 (diff | hist) m Virginia/30 July 2008 (top)
(Latest | Earliest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)
