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User contributions
From 2008.igem.org
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- 18:39, 29 October 2008 (diff | hist) Team:The University of Alberta (→Gold sponsors)
- 18:22, 29 October 2008 (diff | hist) Team:The University of Alberta (→Silver Sponsors)
- 19:37, 28 August 2008 (diff | hist) N Team:The University of Alberta/28 August 2008 (New page: ==Today== *The Site Directed Mutagenesis appears to have worked. We will digest with NotI to find out for sure *ER01 + ER02 ligation transformants grew. Digesting and/or sequencing to make...) (top)
- 20:00, 27 August 2008 (diff | hist) Team:The University of Alberta/27 August 2008 (→Today) (top)
- 16:27, 27 August 2008 (diff | hist) N Team:The University of Alberta/27 August 2008 (New page: ==Today== *Finishing off the site-directed mutagenesis: digesting and transforming *The ER01 and ER02 digestions didnt work: got lots of bands. Will have to repeat. *TetR+RBS ligations did...)
- 17:47, 26 August 2008 (diff | hist) N Team:The University of Alberta/26 August 2008 (New page: ==Today== *The P0380+MCS transformants grew! Making overnights today. *The mutated PR grew as well. Overnights were set up. *Redoing the Site Directed Mutagenesis as follows: **5 reactions...) (top)
- 20:18, 25 August 2008 (diff | hist) Team:The University of Alberta/25 August 2008 (→Today) (top)
- 17:50, 25 August 2008 (diff | hist) N Team:The University of Alberta/25 August 2008 (New page: ==Today== *Transforming the P0380+MCS ligation (#7, #2 and #4 were done using bad P0380 samples) *Transforming mutated Purple Russian into "Blue" cells.)
- 20:14, 22 August 2008 (diff | hist) Team:The University of Alberta/22 August 2008 (top)
- 16:25, 22 August 2008 (diff | hist) N Team:The University of Alberta/22 August 2008 (New page: ==Today== *ER01+ER02 didnt grow again. Dunno why not *Began to insert MCS into binary vector)
- 16:28, 21 August 2008 (diff | hist) N Team:The University of Alberta/21 August 2008 (New page: ==Today== *TetR+RBS transformations didnt work. We did digests of them yesterday just in case so we can transform + ligate them today *Doing transformation of ER01+ER02 *Purple Russian in ...) (top)
- 20:25, 20 August 2008 (diff | hist) m Team:The University of Alberta/20 August 2008 (→Today) (top)
- 16:47, 20 August 2008 (diff | hist) N Team:The University of Alberta/20 August 2008 (New page: ==Today== *The retransformed Site Directed Mutagenesis: Lots o' growth on the transformation control = good! Growth on the Mutagenesis control = good! Growth on the binary vector = very go...)
- 20:28, 19 August 2008 (diff | hist) N Team:The University of Alberta/19 August 2008 (New page: ==Today== *Regarding the Site Directed Mutagenesis: The transformed non-mutated binary vector did grow so we know that it is not too large to transform. *We do think we know the problem: t...) (top)
- 21:54, 18 August 2008 (diff | hist) Team:The University of Alberta/18 August 2008 (→Today) (top)
- 16:14, 18 August 2008 (diff | hist) N Team:The University of Alberta/18 August 2008 (New page: ==Today== *Site Directed Mutagenesis: Got LOTS of growth on the transformation control, a fair amount of growth on the mutagenesis control that was positive so we know that it works! HOWEV...)
- 17:17, 15 August 2008 (diff | hist) N Team:The University of Alberta/15 August 2008 (New page: ==Today== '''Chris''' *The transformations of the mutated binary vector did not grow: neither did the transformation control. Its likely that the transformation simply didnt work. *I am re...)
- 17:12, 15 August 2008 (diff | hist) N Team:The University of Alberta/14 August 2008 (New page: ==Today== '''Chris''' *Finshed off the Site Directed Mutagenesis of our binary vector - David and Kelly transformed the cells in the evening *Made fresh NZY+ broth) (top)
- 21:11, 13 August 2008 (diff | hist) Team:The University of Alberta/13 August 2008 (top)
- 16:51, 12 August 2008 (diff | hist) N File:Bioalberta.jpeg (top)
- 16:51, 12 August 2008 (diff | hist) Team:The University of Alberta
- 16:41, 12 August 2008 (diff | hist) N Team:The University of Alberta/12 August 2008 (New page: ==Today== '''Chris''' *Ligated Tryp into J6. Since the result of the ligase test from yesterday was inconclusive (because we didnt run any controls!) this may not work.)
- 16:39, 12 August 2008 (diff | hist) Team:The University of Alberta/11 August 2008 (→Today) (top)
- 16:21, 11 August 2008 (diff | hist) N Team:The University of Alberta/11 August 2008 (New page: ==Today== '''Chris''' *Got the sequence results back from Friday: Looks like the suspicious band was Tryp after all. Will go ahead with the ligation into Tryp. Ive decided that if it doesn...)
- 16:45, 8 August 2008 (diff | hist) N Team:The University of Alberta/8 August 2008 (New page: '''Chris''' *Ran the sequencing from yesterday (both mine and Jason's, plus one mystery sample that was in the box). Will have the results back tomorrow.)
- 16:43, 8 August 2008 (diff | hist) Team:The University of Alberta/7 August 2008
- 16:43, 8 August 2008 (diff | hist) Team:The University of Alberta/6 August 2008
- 16:42, 8 August 2008 (diff | hist) Team:The University of Alberta/5 August 2008 (→Back to the Lab after the Long Weekend!)
- 16:35, 8 August 2008 (diff | hist) Team:The University of Alberta/6 August 2008
- 16:28, 8 August 2008 (diff | hist) Team:The University of Alberta/5 August 2008 (→Back to the Lab after the Long Weekend!)
- 16:42, 5 August 2008 (diff | hist) N Team:The University of Alberta/5 August 2008 (New page: ==Back to the Lab after the Long Weekend!== '''Chris''' *Winnie redid the ligation of J6 and Tryp. I put transforming this on hold though because... **She also did colony PCR on some more ...)
- 15:19, 1 August 2008 (diff | hist) Team:The University of Alberta
- 16:46, 30 July 2008 (diff | hist) N Team:The University of Alberta/30 July 2008 (New page: ===Today=== '''Chris''' *Running the PCR from the lab water on a gel. Results pending. *Religated J6 and Tryp) (top)
- 16:44, 30 July 2008 (diff | hist) Team:The University of Alberta/29 July 2008 (→Today) (top)
- 21:49, 29 July 2008 (diff | hist) File:Jason.jpg (uploaded a new version of "Image:Jason.jpg") (top)
- 21:43, 29 July 2008 (diff | hist) Team:The University of Alberta/Team (→Students)
- 21:34, 29 July 2008 (diff | hist) Team:The University of Alberta/Team (→Students)
- 17:08, 29 July 2008 (diff | hist) N Team:The University of Alberta/29 July 2008 (New page: ==The Big Whoops== We found the source of the problems we have been having with using NgoMIV and AgeI: the ER0x and BisDx parts dont have the NgoMIV and AgeI sites in them! Not good! We ma...)
- 23:54, 28 July 2008 (diff | hist) Team:The University of Alberta/28 July 2008 (→Today) (top)
- 17:06, 28 July 2008 (diff | hist) Team:The University of Alberta/28 July 2008
- 20:14, 24 July 2008 (diff | hist) Team:The University of Alberta/24 July 2008 (→System 2)
- 20:11, 24 July 2008 (diff | hist) Team:The University of Alberta/24 July 2008 (→System 2)
- 20:17, 21 July 2008 (diff | hist) Team:The University of Alberta/21 July 2008 (→Today)
- 20:09, 21 July 2008 (diff | hist) Team:The University of Alberta/21 July 2008 (→Today)
- 17:02, 21 July 2008 (diff | hist) Team:The University of Alberta/21 July 2008 (→Today)
- 17:01, 21 July 2008 (diff | hist) N Team:The University of Alberta/21 July 2008 (New page: ===Today=== *Transformed the ligation of Tryp into J6 *Finished Westerns of the Butanols suff in J6...RESULTS PENDING)
- 18:09, 18 July 2008 (diff | hist) Team:The University of Alberta/18 July 2008 (→Today)
- 18:09, 18 July 2008 (diff | hist) N Team:The University of Alberta/18 July 2008 (New page: ===Today=== *Got the sequencing back that Winnie did on the BisDA gene: it was LLC because she used the primers; no big deal though because the size was correct and we're pretty sure that ...)
- 18:06, 18 July 2008 (diff | hist) Team:The University of Alberta/17 July 2008 (→Today) (top)
- 17:05, 18 July 2008 (diff | hist) Team:The University of Alberta/17 July 2008 (→Today)
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