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Team:Hawaii/Notebook/2008-07-18
From 2008.igem.org
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
PCR of pRL1383a Parts
- Margaret
- for details please look here.
Extraction of Biobricks and Transformation in DB3.1
- Margaret
- for details please look here.
Sequencing
- Grace
- Redid PCR reactions for GFP fusion, BB-pRL1383a, B0015, J04430, R0010
- Gel OK this time (95V for 50 min; ladder resolved, loading dye didn't run funny)
- Incorrect bands still for B0015, J04430, R0010; Krystle will redo the plasmid preps for these
- Correct bands for GFP fusion and BB-pRL1383a. BB-pRL1383a band faint -- why?
- Treated PCR products w/ ExoSAP
- Determined DNA concentrations using nanodrop spectrometer
- Sent 11 samples to CORE Hawaii for sequencing
Drylab Work
Project Description (Abstract)
- Grace, Krystle, Margaret
- Wrote description of project (abstract)
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
