Team:KULeuven/Project/Output

From 2008.igem.org

• Home
• Reports
• Calendar

• Project abstract
• Meet the team
• Leave a message
• Mail us

• The Team
  Research Labs and Groups Students Instructors Advisors Pictures
• The Project
  Summary Components

  Input Output Filter InverTimer Reset Cell Death Memory

  Safety End Evaluation Literature Brainstorm
• Ethics
  Introduction Three A's

  Defining the three A's Developments in modern physics Developments in biology

  Concerns & Issues

  Defining synthetic biology A framework Concerns Issues The need for answers

  Biological Robotics

  KULeuven iGEM 2008 project Biological robotics

  Afterword References
• Submitted Parts
  Sandbox
• Modeling
  Overview Kinetic Constants Components

  Output Filter InverTimer Reset Cell Death Memory

  Full Model Sensitivity Analysis Multi-cell Model Diffusion
• Data Analysis
  Overview New Parts

  GFP (LVA-tag) Other

  Components

  Input Output Other
• Software
  Multi-cell Toolbox Simbiology2LaTeX Toolbox
• Notebook
  Protocols Reports

  Daily Weekly

  Lab Data

  Freezer Primers Ligation

  Tools Press Acknowledgments

Output

We have been searching for an easy accesible output system, that could us provide in a quick and easy way qualitative and quantitative information. The choice for GFP was quickly made.

BioBricks

Components

We added C-terminal a LVA tag for rapid degradation of the protein, so that we can follow the output signal quickly in time. The OmpF promoter ([http://partsregistry.org/Part:BBa_R0084 BBa_R0084]) behaves as described in the input section. For fine-tuning reasons, the RBS was chosen [http://partsregistry.org/Part:BBa_B0032 BBa_B0032] with a relative efficiency of 0,3.

Action

This is a very easy system. As the input results in a low concentration of phosphorylated OmpR, there is GFP production, proportional to the input signal.

• Recent changes
• What links here
• Related changes
• Special pages
• My preferences

• Printable version
• Permanent link
• Privacy policy
• Disclaimers